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Microfluidics-based point-of-care test for serodiagnosis of Lyme Disease.

Abstract：

:Currently, diagnostic testing for Lyme disease is done by determination of the serologic responses to Borrelia burgdorferi antigens, with the exception of the early localized phase of disease where diagnosis must be done clinically. Here, we describe the use of microfluidics technology to develop a multiplexed rapid lab-on-a-chip point of care (POC) assay for the serologic diagnosis of human Lyme disease. Following ELISA screening of 12 candidate antigens, we tested 8 on a microfluidic diagnostic system, called mChip-Ld, using a set of 60 serological samples. The mChip-Ld test, which can be performed in 15 minutes at the point of care, showed promising performance for detection of antibodies to B. burgdorferi using the PPO triplex test (rP100 + PepVF + rOspC-K, AUC of 0.844) compared to a gold-standard reference of culture confirmed clinical samples. The performance is comparable to the commonly used C6 peptide by lab-based ELISA. In addition, the mChip-Ld test showed promising performance for early-stage diagnosis of the disease using the antigen OspC-K (sensitivity and specificity of 84% and 92%, respectively; AUC of 0.877). Overall, this study underscores the potential of using microfluidics to aid the diagnosis of Lyme disease at the point of care.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Nayak S,Sridhara A,Melo R,Richer L,Chee NH,Kim J,Linder V,Steinmiller D,Sia SK,Gomes-Solecki M

doi

10.1038/srep35069

subject

Has Abstract

pub_date

2016-10-11 00:00:00

pages

35069

issn

2045-2322

pii

srep35069

journal_volume

6

pub_type

杂志文章

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