Abstract:
:Using in vivo second harmonic generation (SHG) and third harmonic generation (THG) microscopies, we tracked the course of collagen remodeling over time in the same melanoma microenvironment within an individual mouse. The corresponding structural and morphological changes were quantitatively analyzed without labeling using an orientation index (OI), the gray level co-occurrence matrix (GLCM) method, and the intensity ratio of THG to SHG (RTHG/SHG). In the early stage of melanoma development, we found that collagen fibers adjacent to a melanoma have increased OI values and SHG intensities. In the late stages, these collagen networks have more directionality and less homogeneity. The corresponding GLCM traces showed oscillation features and the sum of squared fluctuation VarGLCM increased with the tumor sizes. In addition, the THG intensities of the extracellular matrices increased, indicating an enhanced optical inhomogeneity. Multiplying OI, VarGLCM, and RTHG/SHG together, the combinational collagen remodeling (CR) index at 4 weeks post melanoma implantation showed a 400-times higher value than normal ones. These results validate that our quantitative indices of SHG and THG microscopies are sensitive enough to diagnose the collagen remodeling in vivo. We believe these indices have the potential to help the diagnosis of skin cancers in clinical practice.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Wu PC,Hsieh TY,Tsai ZU,Liu TMdoi
10.1038/srep08879subject
Has Abstractpub_date
2015-03-09 00:00:00pages
8879issn
2045-2322pii
srep08879journal_volume
5pub_type
杂志文章abstract::Duchenne muscular dystrophy, one of the most common lethal genetic disorders, is caused by mutations in the DMD gene and a lack of dystrophin protein. In most DMD patients and animal models, sporadic dystrophin-positive muscle fibres, called revertant fibres (RFs), are observed in otherwise dystrophin-negative backgro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38371
更新日期:2016-12-07 00:00:00
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journal_title:Scientific reports
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2013-11-14 00:00:00
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更新日期:2016-08-19 00:00:00
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pub_type: 杂志文章
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更新日期:2016-02-25 00:00:00
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pub_type: 杂志文章
doi:10.1038/srep08338
更新日期:2015-02-09 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Scientific reports
pub_type: 杂志文章
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更新日期:2019-01-09 00:00:00
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journal_title:Scientific reports
pub_type: 杂志文章
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更新日期:2020-02-06 00:00:00
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journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27937
更新日期:2016-06-13 00:00:00
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journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-11985-5
更新日期:2017-09-27 00:00:00