Abstract：
:Post-transcriptional control of gene expression by microRNAs provides an important regulatory system within neurons, allowing co-ordinate and fine-tuned expression of plasticity-related proteins. Indeed, specific microRNAs have been shown to be regulated by synaptic activity in the dentate gyrus, and contribute to the regulated gene expression that underlies the persistence of long-term potentiation (LTP), a model of memory. To fully explore the contribution of microRNAs in synaptic plasticity, it is important to characterize the complete microRNA transcriptome in regions such as the dentate gyrus. Accordingly we used deep sequencing and miRDeep* analysis to search for novel microRNAs expressed in the dentate gyrus granule cell layer. Drawing on combined sequencing and bioinformatics analyses, including hairpin stability and patterns of precursor microRNA processing, we identified nine putative novel microRNAs. We did not find evidence of differential expression of any of these putative microRNAs following LTP at perforant path-granule cell synapses in awake rats (5 h post-tetanus; p > 0.05). Focusing on novel_miR-1, the most abundant novel miRNA, we showed that this sequence could be amplified from RNA extracted from dentate gyrus granule cells by reverse transcription-quantitative polymerase chain reaction. Further, by computationally predicting mRNA targets of this microRNA, we found that this novel microRNA likely contributes to the regulation of proteins that function at synapses.
journal_name
Neurosci Lettjournal_title
Neuroscience lettersauthors
Ryan B,Williams JMdoi
10.1016/j.neulet.2016.09.003subject
Has Abstractpub_date
2019-08-10 00:00:00pages
132280eissn
0304-3940issn
1872-7972pii
S0304-3940(16)30664-4journal_volume
707pub_type
杂志文章abstract：:The origin of sympathetic and sensory innervation of the elbow and knee joint was studied by the intra-axonal transport method. After injection of WGA-HRP into the elbow and knee joint unilaterally in adult monkeys (macaca fascicularis), labelled cells were observed ipsilaterally in both the sympathetic and dorsal roo...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90790-z
更新日期：1991-06-24 00:00:00
abstract：:The aim of the present study was to examine the patterns of activation of the P600 waveform of the event-related potentials (ERP), applying principal component analysis (PCA) and repeated measures ANOVA, and whether these patterns are RF and gender dependent. The ERPs of thirty-nine healthy subjects (20 male and 19 fe...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2010.04.058
更新日期：2010-06-30 00:00:00
abstract：:Early craniectomy has shown to reduce infarction size in experimental large MCA infarction probably due to improved leptomeningeal perfusion. Based on the hypothesis that craniectomy may also be beneficial in smaller MCA infarction we evaluated the effects of craniectomy on infarction size in small thromboembolic cere...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.07.092
更新日期：2004-11-11 00:00:00
abstract：:We investigated the effects of a weak cutaneous stimulus applied to the digital nerves (prepulse) on the masseter inhibitory reflex (MIR) to electrical stimulation of the mental nerve in nine healthy volunteers. The prepulse stimulus, which had no direct effect on the masseter muscle EMG activity, induced a significan...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12574-x
更新日期：1996-04-26 00:00:00
abstract：:gamma-Interferon (IFN-gamma) is known to induce expression of major histocompatibility complex (MHC) class II antigens on murine astrocytes and MHC class I antigens on murine oligodendrocytes. We studied whether the human IFN-gamma could induce the expression of Human Leukocyte Antigen (HLA)-A, B, C and -DR antigens o...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(86)90543-4
更新日期：1986-12-23 00:00:00
abstract：:Recent studies suggested that statins have anti-inflammatory effects beyond their lipid-lowering properties. In the present study, we sought to investigate whether rosuvastatin could alleviate morphine tolerance by attenuating the glia mediated neuroinflammation in the spinal cord. Using a rat model of L5 spinal nerve...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.11.003
更新日期：2015-01-01 00:00:00
abstract：:A comparison of the effect of fatigue caused by electrical induction or voluntary contraction on Ia inhibition in human soleus muscle was investigated in fourteen healthy male subjects. The Hoffmann reflex (H reflex) was employed to elucidate the contribution of fatigue induced contraction to the Ia inhibition. A stat...
journal_title：Neuroscience letters
pub_type： 临床试验,杂志文章
doi：10.1016/0304-3940(95)11883-x
更新日期：1995-09-01 00:00:00
abstract：:Near-infrared spectroscopy (NIRS) is a non-invasive optical technique which has been successfully used to register substantial cerebral ischemia. There are indications that NIRS is sensible enough to detect also small metabolic changes during the performance of cognitive tasks. In the present study, two-channel NIRS o...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(97)13416-4
更新日期：1997-02-21 00:00:00
abstract：:Contradictory results have been reported regarding the participation of bulbospinal inspiratory (BSI) neurons in vomiting contractions of the diaphragm in dogs and cats maintained at low and high end-tidal CO2 levels, respectively. To resolve this discrepancy, effects of CO2 levels on vomiting activities were observed...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(94)90533-9
更新日期：1994-10-24 00:00:00
abstract：:The contribution of retinal and extraretinal signals to the coding of eye position in the head was studied in human subjects (Ss). Horizontal saccades were produced in darkness. For some trials, before returning gaze direction to the starting position, a visual signal briefly stimulated the foveal retina. Results show...
journal_title：Neuroscience letters
pub_type： 临床试验,杂志文章
doi：10.1016/0304-3940(94)11147-b
更新日期：1995-01-09 00:00:00
abstract：:The administration of DN-1417, a synthetic derivative of TRH with more potent central action, significantly reduced specific [3H]GABA binding in the cerebellum, whereas [3H]GABA binding in the cerebral cortex was not changed. Scatchard analysis showed that the decreased [3H]GABA binding in the cerebellum was due to de...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(83)90398-1
更新日期：1983-12-02 00:00:00
abstract：:There is controversy as to what extent the processing of spectrally rich sounds in the human auditory cortex is related to the processing of singular frequencies. An informative index of the function of the auditory cortex, particularly important in neurological patients, is the mismatch negativity (MMN), a component ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2003.08.045
更新日期：2003-12-04 00:00:00
abstract：:Early intervention following combat deployment has the potential to prevent posttraumatic stress disorder (PTSD), but there is a need for greater understanding of the factors that contribute to PTSD symptom progression. This study investigated: (1) fear-potentiated startle during a fear extinction, (2) white matter mi...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2016.02.041
更新日期：2016-04-08 00:00:00
abstract：:Neurofibromatosis 1 (NF1) is a common autosomal dominant disorder in which affected individuals develop benign and malignant tumors as well as non-tumor-related abnormalities, such as seizures and learning disabilities. Here, we report an NF1 isoform arising from the alternative splicing of exon 9a with predominant ce...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(96)12730-0
更新日期：1996-06-21 00:00:00
abstract：:The effects of severe hypoglycemia on perforant path-evoked field potentials were examined in the rat hippocampus. Although a complete loss of this response was noted when blood glucose concentration fell below 1 mM, this occurred before cessation of electroencephalogram (EEG) activity. Both spontaneous and evoked res...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(87)90418-6
更新日期：1987-05-19 00:00:00
abstract：:Our previous studies showed that corticospinal excitability during imagery of squeezing a foam ball was enhanced by somatosensory input generated by passively holding the ball. In the present study, using the same experimental model, we investigated whether corticospinal excitability was influenced by holding the obje...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.02.073
更新日期：2012-04-11 00:00:00
abstract：:Dystrobrevin is a newly discovered dystrophin-associated protein that is classified as alpha- and beta-dystrobrevin. Previous studies reported that dystrophin, utrophin, syntrophin and beta-dystroglycan were expressed in the cerebellum. In the present study, we examined cellular and subcellular localization of dystrob...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(00)00925-3
更新日期：2000-04-07 00:00:00
abstract：:To examine the effect of apolipoprotein E (ApoE) 4 on the progression of Alzheimer's disease (AD), the clinical course of 33 AD patients (17 cases with ApoE epsilon4 and 16 cases without ApoE epsilon4) was evaluated with the mini-mental state examination (MMSE) and cerebrospinal fluid (CSF) biological markers. The dec...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00323-7
更新日期：1999-05-21 00:00:00
abstract：:1,3-di(2-[5-3H]tolyl)Guanidine ([3H]DTG) was found to bind to a single saturable population of binding sites in human cerebral cortex and NCB20 cells, a second low-affinity site was apparent in guinea pig brain. Displacement studies were performed to determine the pharmacology of the [3H]DTG binding site in these 3 me...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(91)90621-y
更新日期：1991-10-14 00:00:00
abstract：:Alzheimer's disease (AD) is still difficult to be precisely diagnosed in its early stage to date. Establishing of reliable and manageable disease-specific biological markers is required to improve diagnostic accuracy. Based on the hypothesis of cell cycle regulatory failure at the early stage of AD, we tested whether ...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.03.093
更新日期：2012-05-16 00:00:00
abstract：:Neurones immunoreactive to an antibody against a pigment-dispersing hormone (PDH-ir) are considered to be pacemaker cells in the neuronal circadian system of flies. We investigated the ultrastructure of PDH-ir varicosities, possible releasing sites of the PDH-like peptide, in the distal medulla of the optic lobe and i...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2004.03.051
更新日期：2004-06-03 00:00:00
abstract：:The pineal product melatonin that acts on specific melatonin receptors has been implicated in pathobiological mechanisms of neuropsychiatric disorders including Alzheimer's disease. We used mice lacking melatonin MT(2) receptors (MT(2) knockouts) to investigate the role of these receptors in synaptic plasticity and le...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2005.09.040
更新日期：2006-01-23 00:00:00
abstract：:In a random number generation (RNG) task subjects are instructed to generate the numbers 1-10 in a random fashion. RNG performance is assumed to involve executive functions, as it requires controlled response generation and suppression of habitual responses. To investigate cerebral structures involved in RNG associate...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(03)00496-8
更新日期：2003-07-10 00:00:00
abstract：:Astrocytic glial cells from neonatal rat brains were grown in primary culture. Dipeptidyl peptidase II (DPP-II) enzyme activity was measured in cells disrupted by nonionic detergent. The rate of enzyme activity was measured in the presence of various ions, under isosmotic conditions adjusted using mannitol and NaCl. D...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90546-0
更新日期：1988-07-08 00:00:00
abstract：:Mounting evidence suggests a chronic pro-inflammatory state in individuals with bipolar disorder (BD). Stress exposure is known to exacerbate several inflammatory conditions as well as psychiatric disorders. Here, we analyzed plasma levels of pro-inflammatory cytokines and their soluble receptors to realistic acute ps...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2014.07.040
更新日期：2014-09-19 00:00:00
abstract：:The effects of Sr2+ on the transmitter release at the frog neuromuscular junction were examined electrophysiologically. The nerve trunk was stimulated by paired pulses at various time intervals after replacing extracellular Ca2+ by Sr2+, the paired-pulse facilitation at the 10 ms interval was smaller than that at 20-3...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/s0304-3940(99)00767-3
更新日期：1999-11-19 00:00:00
abstract：:Evidence indicates that sympathetic nerves and substance P (SP) are involved in some physiological and pathophysiological changes and activities in retina. The aim of this study was to investigate whether SP participates in the stress reaction and possible involvement of adrenergic mechanisms in modulation of the chan...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2008.07.073
更新日期：2008-10-10 00:00:00
abstract：:The cyclic AMP/protein kinase A signaling pathway is thought to be involved in neural differentiation of mesenchymal stem cells. In the present study, we examined the involvement of β-adrenoceptor signaling on the differentiation of mouse induced pluripotent stem (iPS) cells into neural progenitor cells. Mouse iPS cel...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/j.neulet.2012.07.028
更新日期：2012-09-06 00:00:00
abstract：:We have previously shown that treatment of cultured dorsal root ganglion neurons (DRGN) with a highly specific, reversible acetylcholinesterase (AChE) inhibitor, BW284c51, retards neuritic outgrowth in a dose dependent manner and is accompanied by the presence of abnormal, perikaryal neurofilament (NF) inclusions in a...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(95)11895-4
更新日期：1995-09-01 00:00:00
abstract：:Recent studies have described the role of various regions of the neural cell adhesion molecule (NCAM) in cell-cell interactions. Monoclonal antibodies (L2/HNK-1) directed against a sulfated, glucuronic acid-containing, N-linked carbohydrate epitope have also been shown to inhibit NCAM-mediated neural cell adhesion. In...
journal_title：Neuroscience letters
pub_type： 杂志文章
doi：10.1016/0304-3940(88)90076-6
更新日期：1988-11-11 00:00:00
