Abstract:
:Myeloid-derived suppressor cells (MDSCs) are highly prevalent inflammatory cells that play a key role in tumor development and are considered therapeutic targets. MDSCs promote tumor growth by blocking T-cell-mediated anti-tumoral immune response through depletion of arginine that is essential for T-cell proliferation. To deplete arginine, MDSCs express high levels of arginase, which catalyzes the breakdown of arginine into urea and ornithine. Here, we developed a new hyperpolarized (13)C probe, [6-(13)C]-arginine, to image arginase activity. We show that [6-(13)C]-arginine can be hyperpolarized, and hyperpolarized [(13)C]-urea production from [6-(13)C]-arginine is linearly correlated with arginase concentration in vitro. Furthermore we show that we can detect a statistically significant increase in hyperpolarized [(13)C]-urea production in MDSCs when compared to control bone marrow cells. This increase was associated with an increase in intracellular arginase concentration detected using a spectrophotometric assay. Hyperpolarized [6-(13)C]-arginine could therefore serve to image tumoral MDSC function and more broadly M2-like macrophages.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Najac C,Chaumeil MM,Kohanbash G,Guglielmetti C,Gordon JW,Okada H,Ronen SMdoi
10.1038/srep31397subject
Has Abstractpub_date
2016-08-10 00:00:00pages
31397issn
2045-2322pii
srep31397journal_volume
6pub_type
杂志文章abstract::Little is known on the causes and pathogenesis of the adipose tissue disorder (familial) Multiple Symmetric Lipomatosis (MSL). In a four-generation MSL-family, we performed whole exome sequencing (WES) in 3 affected individuals and 1 obligate carrier and identified Calcyphosine-like (CAPSL) as the most promising candi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-44382-1
更新日期:2019-06-11 00:00:00
abstract::High-entropy alloys (HEAs) have inspired considerable interest due to their attractive physical and mechanical properties. In this work, the microstructural evolution induced by different heat treatments on rapidly solidified hypoeutectic precursors of a Fe26.7Co26.7Ni26.7Si8.9B11 HEA is investigated and correlated wi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-36464-3
更新日期:2019-01-23 00:00:00
abstract::Circular Dichroism data are often decomposed into their constituent spectra to quantify the secondary structure of peptides or proteins but the estimation of the secondary structure content fails when light scattering leads to spectral distortion. If peptide-induced liposome self-association occurs, subtracting contro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39450
更新日期:2016-12-22 00:00:00
abstract::Chronic sleep fragmentation (SF) commonly occurs in human populations, and although it does not involve circadian shifts or sleep deprivation, it markedly alters feeding behaviors ultimately promoting obesity and insulin resistance. These symptoms are known to be related to the host gut microbiota. Mice were exposed t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep35405
更新日期:2016-10-14 00:00:00
abstract::Black phosphorus (BP) has recently been rediscovered as a new and interesting two-dimensional material due to its unique electronic and optical properties. Here, we study the linear and nonlinear optical properties of BP flakes. We observe that both the linear and nonlinear optical properties are anisotropic and can b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15899
更新日期:2015-10-30 00:00:00
abstract::The mammalian Major Histocompatibility Complex (MHC) region contains several gene families characterized by highly polymorphic loci with extensive nucleotide diversity, copy number variation of paralogous genes, and long repetitive sequences. This structural complexity has made it difficult to construct a reliable ref...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep45518
更新日期:2017-03-31 00:00:00
abstract::To describe the rationale and surgical technique and compare the clinical effect of posterior percutaneous endoscopic cervical discectomy (PPECD) using the Delta system versus that of conventional PPECD (key-hole) surgery for the treatment of symptomatic cervical spondylotic radiculopathy (CSR). A retrospective analys...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67381-z
更新日期:2020-06-24 00:00:00
abstract::Measurements of skeletal muscle cross-sectional area, index, and radiation attenuation utilizing clinical computed tomography (CT) scans are used in assessments of sarcopenia, the loss of skeletal muscle mass and function associated with aging. To classify individuals as sarcopenic, sex-specific cutoffs for 'low' valu...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-29825-5
更新日期:2018-07-27 00:00:00
abstract::The propagation of Toxoplasma gondii is accomplished by repeated lytic cycles of parasite attachment to a host cell, invasion, replication within a parasitophorous vacuole, and egress from the cell. This lytic cycle is delicately regulated by calcium-dependent reversible phosphorylation of the molecular machinery that...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-42441-1
更新日期:2019-04-11 00:00:00
abstract::Epidemiological studies using saliva have revealed relationships between the oral microbiome and many oral and systemic diseases. However, when collecting from a large number of participants such as a large-scale cohort study, the time it takes to collect saliva can be a problem. Mouth-rinsed water, which is water tha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52445-6
更新日期:2019-11-06 00:00:00
abstract::Tsetse flies are the transmitting vector of trypanosomes causing human sleeping sickness and animal trypanosomiasis in sub-saharan Africa. 3-alkylphenols are used as attractants in tsetse fly traps to reduce the spread of the disease. Here we present an inexpensive production method for 3-ethylphenol (3-EP) and 3-prop...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66997-5
更新日期:2020-06-19 00:00:00
abstract::Mechanotransduction is crucial for touch sensation, hearing, proprioception, and pain sensing. In C. elegans, male ray neurons have been implicated to be involved in the mechanosensation required for mating behavior. However, whether ray neurons directly sense mechanical stimulation is not yet known, and the underlyin...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-25542-1
更新日期:2018-05-08 00:00:00
abstract::For a broad class of input-output maps, arguments based on the coding theorem from algorithmic information theory (AIT) predict that simple (low Kolmogorov complexity) outputs are exponentially more likely to occur upon uniform random sampling of inputs than complex outputs are. Here, we derive probability bounds that...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61135-7
更新日期:2020-03-10 00:00:00
abstract::Outcomes following admission to intensive care units (ICU) may vary with time and day. This study investigated associations between time of day and risk of ICU mortality and chance of ICU discharge in acute ICU admissions. Adult patients (age ≥ 18 years) who were admitted to ICUs participating in the Austrian intensiv...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-48947-y
更新日期:2019-08-29 00:00:00
abstract::Fermentation is the key process to produce the special color of congou black tea. The machine vision technology is applied to detect the color space changes of black tea's color in RGB, Lab and HSV, and to find out its relevance to black tea's fermentation quality. And then the color feature parameter is used as input...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28767-2
更新日期:2018-07-12 00:00:00
abstract::Predicting the hosts of newly discovered viruses is important for pandemic surveillance of infectious diseases. We investigated the use of alignment-based and alignment-free methods and support vector machine using mononucleotide frequency and dinucleotide bias to predict the hosts of viruses, and applied these approa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28308-x
更新日期:2018-07-03 00:00:00
abstract::The starch granule is Nature's way to store energy in green plants over long periods. Irrespective of their origins, starches display distinct structural features that are the fingerprints of levels of organization over six orders of magnitude. We hypothesized that Nature retains hierarchical material structures at al...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-72218-w
更新日期:2020-11-18 00:00:00
abstract::Male specific DNA sequences were selected from a Diversity Arrays Technology (DArT) mapping study to evaluate their suitability for determination of the sex phenotype among young seedlings in a hop (Humulus lupulus L.) breeding program. Ten male specific DArT markers showed complete linkage with male sex phenotype in ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50400-z
更新日期:2019-10-02 00:00:00
abstract::The Batrachosauroididae are an enigmatic group of salamanders known from the Cretaceous and Tertiary of North America and Europe. In Europe, the family is known only by two species of the genus Palaeoproteus. The genus has limited distribution in Western and Central Europe. In the present paper, we describe a new spec...
journal_title:Scientific reports
pub_type: 历史文章,杂志文章
doi:10.1038/s41598-020-59255-1
更新日期:2020-02-17 00:00:00
abstract::Direct visualization and assessment of the iridocorneal angle (ICA) region with high resolution is important for the clinical evaluation of glaucoma. However, the current clinical imaging systems for ICA do not provide sufficient structural details due to their poor resolution. The key challenges in achieving high qua...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30844
更新日期:2016-07-29 00:00:00
abstract::Wheat (Triticum aestivum), one of the world's most important crops, is facing unprecedented challenges due to global warming. To evaluate the gene resources for heat adaptation in hexaploid wheat, small heat shock proteins (sHSPs), the key plant heat protection genes, were comprehensively analysed in wheat and related...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-01857-3
更新日期:2017-05-31 00:00:00
abstract::It has been demonstrated that development of three-dimensional printing technology has supported the researchers and surgeons to apply the bone tissue engineering to the oromandibular reconstruction. In this study, poly caprolactone/beta tricalcium phosphate (PCL/β-TCP) scaffolds were fabricated by multi-head depositi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-61944-w
更新日期:2020-03-18 00:00:00
abstract::Maintaining vigilance over long periods of time is especially critical in performing fundamental everyday activities and highly responsible professional tasks (e.g., driving, performing surgery or piloting). Here, we investigated the role of aerobic fitness as a crucial factor related to the vigilance capacity. To thi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep27556
更新日期:2016-06-08 00:00:00
abstract::More than a hundred different Transthyretin (TTR) mutations are associated with fatal systemic amyloidoses. They destabilize the protein tetrameric structure and promote the extracellular deposition of TTR as pathological amyloid fibrils. So far, only mutations R104H and T119M have been shown to stabilize significantl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep44709
更新日期:2017-03-24 00:00:00
abstract::Organisms possess multiple DNA polymerases (Pols) and use each for a different purpose. One of the five Pols in Escherichia coli, DNA polymerase IV (Pol IV), encoded by the dinB gene, is known to participate in lesion bypass at certain DNA adducts. To understand how cells choose Pols when the replication fork encounte...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13080-1
更新日期:2017-10-16 00:00:00
abstract::High-throughput single-stranded DNA sequencing (ssDNA-seq) of cell-free DNA from plasma and other bodily fluids is a powerful method for non-invasive prenatal testing, and diagnosis of cancers and other diseases. Here, we developed a facile ssDNA-seq method, which exploits a novel template-switching activity of thermo...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09064-w
更新日期:2017-08-21 00:00:00
abstract::Quantitative reverse transcription PCR (RT-qPCR) is one of the most efficient, reliable and widely used techniques to quantify gene expression. In this study, we evaluated the performance of six southern corn rootworm, Diabrotica undecimpunctata howardi (Barber), housekeeping genes (HKG), β-actin (Actin), β-tubulin (T...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47020-y
更新日期:2019-07-24 00:00:00
abstract::In this study, membrane filtration tests showed that the membrane rejection degree of difloxacin hydrochloride (DFHC) increased significantly in the presence of Suwannee River DOM or Aldrich humic acid (2-10 mg-C/L). Titration experiments showed that the excitation and emission of Peak R belonging to DFHC exhibited bl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-05635-z
更新日期:2017-07-14 00:00:00
abstract::Perinatal depression involves interplay between individual chronic and acute disease burdens, biological and psychosocial environmental and behavioural factors. Here we explored the predictive potential of specific psycho-socio-demographic characteristics for antenatal and postpartum depression symptoms and contributi...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-30874-z
更新日期:2018-08-24 00:00:00
abstract::With aging, less blue light reaches the retina due to gradual yellowing of the lens. This could result in reduced activation of blue light-sensitive melanopsin-containing retinal ganglion cells, which mediate non-visual light responses (e.g., the pupillary light reflex, melatonin suppression, and circadian resetting)....
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep43832
更新日期:2017-03-07 00:00:00