Abstract：
:More than a hundred different Transthyretin (TTR) mutations are associated with fatal systemic amyloidoses. They destabilize the protein tetrameric structure and promote the extracellular deposition of TTR as pathological amyloid fibrils. So far, only mutations R104H and T119M have been shown to stabilize significantly TTR, acting as disease suppressors. We describe a novel A108V non-pathogenic mutation found in a Portuguese subject. This variant is more stable than wild type TTR both in vitro and in human plasma, a feature that prevents its aggregation. The crystal structure of A108V reveals that this stabilization comes from novel intra and inter subunit contacts involving the thyroxine (T4) binding site. Exploiting this observation, we engineered a A108I mutation that fills the T4 binding cavity, as evidenced in the crystal structure. This synthetic protein becomes one of the most stable TTR variants described so far, with potential application in gene and protein replacement therapies.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Sant'Anna R,Almeida MR,Varejāo N,Gallego P,Esperante S,Ferreira P,Pereira-Henriques A,Palhano FL,de Carvalho M,Foguel D,Reverter D,Saraiva MJ,Ventura Sdoi
10.1038/srep44709subject
Has Abstractpub_date
2017-03-24 00:00:00pages
44709issn
2045-2322pii
srep44709journal_volume
7pub_type
杂志文章abstract：:There is a significant need to develop antibacterial materials that could be applied locally and directly to the places surrounded by large amount of bacteria, in order to address the problems of bacterial antibiotic-resistance or irreversible biofilm formation. Hydrogels are thought to be suitable candidates due to t...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep07753
更新日期：2015-01-13 00:00:00
abstract：:Meromictic lakes are useful biogeochemical models because of their stratified chemical gradients and separation of redox reactions down the water column. Perennially ice-covered meromictic lakes are particularly stable, with long term constancy in their density profiles. Here we sampled Lake A, a deep meromictic lake ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00604
更新日期：2012-01-01 00:00:00
abstract：:In this study, we prepared, characterized, and performed toxicity analyses of poly(ε-caprolactone) nanocapsules loaded with neem oil. Three formulations were prepared by the emulsion/solvent evaporation method. The nanocapsules showed a mean size distribution around 400 nm, with polydispersity below 0.2 and were stabl...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-06092-4
更新日期：2017-07-19 00:00:00
abstract：:Glycoprotein nonmetastatic melanoma protein B (GPNMB) plays important roles in various types of cancer and amyotrophic lateral sclerosis (ALS). The details of GPNMB function and its interacting protein have not been clarified. Therefore, to identify GPNMB binding partners on the cell membrane, we used membrane protein...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep23241
更新日期：2016-03-18 00:00:00
abstract：:Emotional expressions of others are salient biological stimuli that automatically capture attention and prepare us for action. We investigated the early cortical dynamics of automatic visual discrimination of fearful body expressions by monitoring cortical activity using magnetoencephalography. We show that right pari...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep24831
更新日期：2016-04-20 00:00:00
abstract：:Lonicera japonica Thunb, rich in chlorogenic acid (CHA), is used for viral upper respiratory tract infection treatment caused by influenza virus, parainfluenza virus, and respiratory syncytial virus, ect in China. It was reported that CHA reduced serum hepatitis B virus level and death rate of influenza virus-infected...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep45723
更新日期：2017-04-10 00:00:00
abstract：:Clinical studies have suggested association of some hepatitis C virus (HCV) subtypes or isolates with progression toward hepatocellular carcinoma (HCC). HCV core protein has been reported to interfere with host Wnt/β-catenin pathway, a cell fate-determining pathway, which plays a major role in HCC. Here, we investigat...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-29078-2
更新日期：2018-07-25 00:00:00
abstract：:Adult zebrafish have the ability to regenerate cells of the central nervous system. However, few neuronal regeneration studies in adult zebrafish addressed their ability to regenerate specific types of neurons following cell specific ablation. We show here that treatment of transgenic Tg(dat:CFP-NTR) adult zebrafish w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-69734-0
更新日期：2020-07-30 00:00:00
abstract：:Deforestation and forest degradation around the world endanger the functioning of ecosystems, climate stability, and conservation of biodiversity. We assessed the spatial and temporal dynamics of forest cover in Myanmar's Hkakabo Razi Landscape (HRL) to determine its integrity based on forest change and fragmentation ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-70917-y
更新日期：2020-08-19 00:00:00
abstract：:This study sought to compare the image quality and radiation dose of coronary computed tomography angiography (CCTA) from prospectively triggered 128-slice CT (128-MSCT) versus dual-source 64-slice CT (DSCT). The study was approved by the Medical Ethics Committee at Tongji Medical College of Huazhong University of Sci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep34795
更新日期：2016-10-18 00:00:00
abstract：:In the last 40 years only one new antitubercular drug has been approved, whilst resistance to current drugs, including rifampicin, is spreading. Here, we used the model organism Mycobacterium smegmatis to study mechanisms of phenotypic mycobacterial resistance, employing quantitative mass spectrometry-based proteomics...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep43858
更新日期：2017-03-06 00:00:00
abstract：:Human umbilical cord mesenchymal stem cells (huMSCs) can treat primary ovarian insufficiency (POI) related to ovarian granulosa cell (OGC) apoptosis caused by cisplatin chemotherapy. Exosomes are a class of membranous vesicles with diameters of 30-200 nm that are constitutively released by eukaryotic cells. Exosomes m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-02786-x
更新日期：2017-05-31 00:00:00
abstract：:Performing everyday actions requires fine postural control, which is a major focus of functional rehabilitation programs. Among the various range of training methods likely to improve balance and postural stability, motor imagery practice (MIP) yielded promising results. Transcranial direct current stimulation (tDCS) ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00509-w
更新日期：2017-03-28 00:00:00
abstract：:Magnetic refrigeration is of great interest due to its high energy efficiency, environmental friendliness and low cost. However, undesired hysteresis losses, concentrated working temperature interval (WTI) and poor mechanical stability are vital drawbacks that hinder its practical application. Off-stoichiometric Ni-Mn...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-35028-9
更新日期：2018-11-08 00:00:00
abstract：:During locomotion, the human ankle-foot system dynamically alters its gearing, or leverage of the ankle joint on the ground. Shifting ankle-foot gearing regulates speed of plantarflexor (i.e., calf muscle) contraction, which influences economy of force production. Here, we tested the hypothesis that manipulating ankle...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-65626-5
更新日期：2020-05-29 00:00:00
abstract：:The near infrared (NIR) absorption and average particle size of gold nanostars (GNSs) can be precisely controlled by varying the molar ratios of cucurbit[7]urils (CB[7]) and GNSs in aqueous solution. GNSs modified with CB[7] achieved high cargo loading with thermally activated release upon the NIR laser irradiation. ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22239
更新日期：2016-02-26 00:00:00
abstract：:Changes in climate and atmospheric CO2 and nitrogen (N) over the last several decades have induced significant effects on forest carbon (C) cycling. However, contributions of individual factors are largely unknown because of the lack of long observational data and the undifferentiating between intrinsic factors and ex...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep04461
更新日期：2014-03-25 00:00:00
abstract：:CHF5633 is a novel synthetic clinical pulmonary surfactant preparation composed by two phospholipid species, dipalmitoyl phosphatidylcholine (DPPC) and palmitoyloleoyl phosphatidylglycerol (POPG), and synthetic analogues of the hydrophobic surfactant proteins SP-B and SP-C. In this study, the interfacial properties of...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-58248-4
更新日期：2020-01-28 00:00:00
abstract：:In this study, a rational combination of 200 pre-selected Carbohydrate-Active enzymes (CAZymes) and sulfatases were tested, individually or combined, according to their ability to degrade Chlorella vulgaris cell wall to access its valuable nutritional compounds. The disruption of microalgae cell walls by a four-enzyme...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-41775-0
更新日期：2019-03-29 00:00:00
abstract：:There are no clinical guidelines for the timing of cholecystectomy (CCY) after performing therapeutic endoscopic retrograde cholangiopancreatography (ERCP) for choledocholithiasis. We tried to analyze the clinical practice patterns, medical expenses, and subsequent outcomes between the early CCY, delayed CCY, and no C...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-38428-z
更新日期：2019-02-18 00:00:00
abstract：:Dichapetalum cymosum produces the toxic fluorinated metabolite, fluoroacetate, presumably as a defence mechanism. Given the rarity of fluorinated metabolites in nature, the biosynthetic origin and function of fluoroacetate have been of particular interest. However, the mechanism for fluorination in D. cymosum was neve...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-77598-7
更新日期：2020-11-25 00:00:00
abstract：:Genetic characterization of local breeds is essential to preserve their genomic variability, to advance conservation policies and to contribute to their promotion and sustainability. Genomic diversity of twenty European local pig breeds and a small sample of Spanish wild pigs was assessed using high density SNP chips....
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-49830-6
更新日期：2019-09-19 00:00:00
abstract：:Increasing evidence indicates that static magnetic fields (SMF) reduce cortical activity in both human and animal models. The aim of this work was to investigate the effect of SMF on epileptiform cortical activity, a condition related to an abnormal increase in neuronal excitability. The first experimental block inclu...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-33808-x
更新日期：2018-10-30 00:00:00
abstract：:Staphylococcus aureus can cause different types of diseases from mild skin infections to life-threatening sepsis worldwide. Owing to the emergence and transmission of multidrug-resistant strains, developing an impactful immunotherapy especially vaccine control approach against S. aureus infections is increasingly enco...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-22044-y
更新日期：2018-02-26 00:00:00
abstract：:There is relatively little literature on adjuvant radiotherapy after radical nephroureterectomy with bladder cuff excision (RNU) for patients with upper tract urothelial carcinoma (UTUC). This study was designed to determine the efficacy of adjuvant radiotherapy for patients with pT3N0M0 UTUC. We retrospectively revie...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep38175
更新日期：2016-12-02 00:00:00
abstract：:Rheumatoid Arthritis (RA) causes chronic inflammation of joints. The cytokines TNFα and IFNγ are central players in RA, however their source has not been fully elucidated. Natural Killer (NK) cells are best known for their role in elimination of viral-infected and transformed cells, and they secrete pro-inflammatory c...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-37448-z
更新日期：2019-02-04 00:00:00
abstract：:Mesoporous silica is an emerging technology to solve problems of existing and to support projected revolutionary applications ranging from targeted drug delivery to artificial kidney. However, one of the major driving mechanisms, electric charging of internal mesoporous surfaces, has not been characterized yet. In the...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36487-w
更新日期：2019-01-15 00:00:00
abstract：:There is currently a lack of effective drugs to cure recurrent aphthous stomatitis. This study aimed to evaluate the efficacy of probiotics alone or as an adjunct in recurrent aphthous stomatitis (RAS) patients. Seven randomized controlled trials (RCTs) were included, of which three were included in quantitative analy...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-78281-7
更新日期：2020-12-03 00:00:00
abstract：:Allicin (diallylthiosulfinate) is a defence molecule from garlic (Allium sativum L.) with broad antimicrobial activities in the low µM range against Gram-positive and -negative bacteria, including antibiotic resistant strains, and fungi. Allicin reacts with thiol groups and can inactivate essential enzymes. However, a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-25154-9
更新日期：2018-04-30 00:00:00
abstract：:Routers, switches, and repeaters are essential components of modern information-processing systems. Similar devices will be needed in future superconducting quantum computers. In this work we investigate experimentally the time evolution of Autler-Townes splitting in a superconducting phase qubit under the application...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep00645
更新日期：2012-01-01 00:00:00
