Abstract:
:In this study, a rational combination of 200 pre-selected Carbohydrate-Active enzymes (CAZymes) and sulfatases were tested, individually or combined, according to their ability to degrade Chlorella vulgaris cell wall to access its valuable nutritional compounds. The disruption of microalgae cell walls by a four-enzyme mixture (Mix) in comparison with the control, enabled to release up to 1.21 g/L of reducing sugars (p < 0.001), led to an eight-fold increase in oligosaccharides release (p < 0.001), and reduced the fluorescence intensity by 47% after staining with Calcofluor White (p < 0.001). The Mix treatment was successful in releasing proteins (p < 0.001), some MUFA (p < 0.05), and the beneficial 18:3n-3 fatty acid (p < 0.05). Even if no variation was detected for chlorophylls (p > 0.05), total carotenoids were increased in the supernatant (p < 0.05) from the Mix treatment, relative to the control. Taken together, these results indicate that this four-enzyme Mix displays an effective capacity to degrade C. vulgaris cell wall. Thus, these enzymes may constitute a good approach to improve the bioavailability of C. vulgaris nutrients for monogastric diets, in particular, and to facilitate the cost-effective use of microalgae by the feed industry, in general.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Coelho D,Lopes PA,Cardoso V,Ponte P,Brás J,Madeira MS,Alfaia CM,Bandarra NM,Gerken HG,Fontes CMGA,Prates JAMdoi
10.1038/s41598-019-41775-0subject
Has Abstractpub_date
2019-03-29 00:00:00pages
5382issue
1issn
2045-2322pii
10.1038/s41598-019-41775-0journal_volume
9pub_type
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