Abstract:
:Light chain (AL) amyloidosis, caused by deposition of amyloidogenic immunoglobulin light chains (LCs), is the most common systemic form in industrialized countries. Still open questions, and premises for developing targeted therapies, concern the mechanisms of amyloid formation in vivo and the bases of organ targeting and dysfunction. Investigating amyloid material in its natural environment is crucial to obtain new insights on the molecular features of fibrillar deposits at individual level. To this aim, we used Fourier transform infrared (FTIR) microspectroscopy for studying in situ unfixed tissues (heart and subcutaneous abdominal fat) from patients affected by AL amyloidosis. We compared the infrared response of affected tissues with that of ex vivo and in vitro fibrils obtained from the pathogenic LC derived from one patient, as well as with that of non amyloid-affected tissues. We demonstrated that the IR marker band of intermolecular β-sheets, typical of protein aggregates, can be detected in situ in LC amyloid-affected tissues, and that FTIR microspectroscopy allows exploring the inter- and intra-sample heterogeneity. We extended the infrared analysis to the characterization of other biomolecules embedded within the amyloid deposits, finding an IR pattern that discloses a possible role of lipids, collagen and glycosaminoglycans in amyloid deposition in vivo.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ami D,Lavatelli F,Rognoni P,Palladini G,Raimondi S,Giorgetti S,Monti L,Doglia SM,Natalello A,Merlini Gdoi
10.1038/srep29096subject
Has Abstractpub_date
2016-07-04 00:00:00pages
29096issn
2045-2322pii
srep29096journal_volume
6pub_type
杂志文章abstract::Magnesium-based thermoelectric materials (Mg2X, X = Si, Ge, Sn) have received considerable attention due to their availability, low toxicity, and reasonably good thermoelectric performance. The synthesis of these materials with high purity is challenging, however, due to the reactive nature and high vapour pressure of...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-04348-7
更新日期:2017-06-21 00:00:00
abstract::Adipocyte differentiation and function relies on a network of transcription factors, which is disrupted in obesity-associated low grade, chronic inflammation leading to adipose tissue dysfunction. In this context, there is a need for a thorough understanding of the transcriptional regulatory network involved in adipos...
journal_title:Scientific reports
pub_type: 临床试验,杂志文章
doi:10.1038/s41598-017-14570-y
更新日期:2017-10-26 00:00:00
abstract::Accurately segmenting foods from optical images is a challenging task, yet becoming possible with the help of recent advances in Deep Learning based solutions. Automated identification of food items opens up possibilities of useful applications like nutrition intake monitoring. Given large variations in food choices, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-79677-1
更新日期:2021-01-12 00:00:00
abstract::The global incidence of obesity has led to an increasing need for understanding the molecular mechanisms that drive this epidemic and its comorbidities. Quantitative real-time RT-PCR (RT-qPCR) is the most reliable and widely used method for gene expression analysis. The selection of suitable reference genes (RGs) is c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-03730-9
更新日期:2017-06-15 00:00:00
abstract::Exosomes contain cell-specific collections of bioactive materials including proteins, lipids, and RNAs that are transported to recipient cells to exert their impacts. MicroRNAs (miRNAs) can function as tumor suppressor or oncogenic genes and miR-21 is one of the most frequently up-regulated miRNAs in solid tumors incl...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-65207-6
更新日期:2020-05-19 00:00:00
abstract::While the worldwide standardization of agricultural production has been advocated and recommended, relatively little research has focused on the ecological significance of such a shift. The ecological concerns stemming from the standardization of agricultural production may require new methodology. In this study, we c...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21675
更新日期:2016-02-22 00:00:00
abstract::Spectroscopic techniques combined with chemometrics methods have proven to be effective tools for the discrimination of objects with similar properties. In this work, terahertz time-domain spectroscopy (THz-TDS) combined with discriminate analysis (DA) and principal component analysis (PCA) with derivative pretreatmen...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep11115
更新日期:2015-07-08 00:00:00
abstract::The association of seasonal trivalent influenza vaccine (TIV) with increased infection by 2009 pandemic H1N1 (A(H1N1)pdm09) virus, initially observed in Canada, has elicited numerous investigations on the possibility of vaccine-associated enhanced disease, but the potential mechanisms remain largely unresolved. Here, ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep37341
更新日期:2016-11-16 00:00:00
abstract::Complex systems displaying recurrent spike patterns are ubiquitous in nature. Understanding the organization of these patterns is a challenging task. Here we study experimentally the spiking output of a semiconductor laser with feedback. By using symbolic analysis we unveil a nontrivial organization of patterns, revea...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep04696
更新日期:2014-04-15 00:00:00
abstract::Functional DNA sub-sequences and genome elements are spatially clustered through the genome just as keywords in literary texts. Therefore, some of the methods for ranking words in texts can also be used to compare different DNA sub-sequences. In analogy with the literary texts, here we claim that the distribution of d...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41543
更新日期:2017-01-27 00:00:00
abstract::Neurotransmitter release from the presynaptic terminal is under very precise spatial and temporal control. Following neurotransmitter release, synaptic vesicles are recycled by endocytosis and refilled with neurotransmitter. During the exocytosis event leading to release, SNARE proteins provide most of the mechanical ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep17669
更新日期:2015-12-04 00:00:00
abstract::The increasing incidence of obesity accentuates the importance of identifying mechanisms and optimal therapeutic strategies for patients with heart failure (HF) in relation to obesity status. Here, we investigated the association between plasma level of apelin, an adipocyte-derived factor, and clinicopathological feat...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16104
更新日期:2015-11-06 00:00:00
abstract::A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has not been fixed in the paper. ...
journal_title:Scientific reports
pub_type: 已发布勘误
doi:10.1038/s41598-018-24113-8
更新日期:2018-04-20 00:00:00
abstract::As a potential alternative to antibiotics, phages can be used to treat multi-drug resistant bacteria. As such, the biological characteristics of phages should be investigated to utilize them as effective antimicrobial agents. In this study, phage PaoP5, a lytic virus that infects Pseudomonas aeruginosa PAO1, was isola...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34067
更新日期:2016-09-23 00:00:00
abstract::Amplicon based metabarcoding promises rapid and cost-efficient analyses of species composition. However, it is disputed whether abundance estimates can be derived from metabarcoding due to taxon specific PCR amplification biases. PCR-free approaches have been suggested to mitigate this problem, but come with considera...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17333-x
更新日期:2017-12-15 00:00:00
abstract::Cortical spreading depolarization (SD) involves activation of NMDA receptors and elicit neurovascular unit dysfunction. NMDA cannot trigger SD in newborns, thus its effect on neurovascular function is not confounded by other aspects of SD. The present study investigated if NMDA affected hypercapnia-induced microvascul...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-55468-1
更新日期:2019-12-11 00:00:00
abstract::The identification of enhancer-target gene (ETG) pairs is vital for the understanding of gene transcriptional regulation. Experimental approaches such as Hi-C have generated valuable resources of ETG pairs. Several computational methods have also been developed to successfully predict ETG interactions. Despite these p...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30043
更新日期:2016-07-20 00:00:00
abstract::Nonalcoholic fibrosing steatohepatitis is a uniform process throughout nonalcoholic fatty liver disease (NAFLD). MicroRNAs (miRNAs) have been suggested to modulate cellular processes in liver diseases. However, the functional role of miRNAs in nonalcoholic fibrosing steatohepatitis is largely unclear. In this study, w...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16163
更新日期:2015-11-05 00:00:00
abstract::Advanced age is associated with a decline in cognitive function, likely caused by a combination of modifiable and non-modifiable factors such as genetics and lifestyle choices. Mounting evidence suggests that humanin and other mitochondrial derived peptides play a role in several age-related conditions including neuro...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32616-7
更新日期:2018-09-21 00:00:00
abstract::It is highly possible that copy number variations (CNVs) in susceptible regions have effects on chronic obstructive pulmonary disease (COPD) development, while long noncoding RNA (lncRNAs) have been shown to cause COPD. We hypothesized that the common CNV, named nsv823469 located on 6p22.1, and covering lncRNAs (major...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep40060
更新日期:2017-01-12 00:00:00
abstract::The Monte Carlo (MC) method is widely used to solve various problems in radiotherapy. There has been an impetus to accelerate MC simulation on GPUs whereas thread divergence remains a major issue for MC codes based on acceptance-rejection sampling. Inverse transform sampling has the potential to eliminate thread diver...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-66844-7
更新日期:2020-06-17 00:00:00
abstract::It is a common belief that reptile eggs should not be turned after oviposition once the embryo has attached itself to the inner membrane of the shell as it might kill developing embryos. Here, we used 338 eggs from 32 clutches of the water snake Natrix maura to (1) thoroughly describe natural clutch arrangement, (2) e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep13385
更新日期:2015-08-21 00:00:00
abstract::WD40 proteins belong to a big protein family with members identified in every eukaryotic proteome. However, WD40 proteins were only reported in a few prokaryotic proteomes. Using WDSP ( http://wu.scbb.pkusz.edu.cn/wdsp/ ), a prediction tool, we identified thousands of prokaryotic WD40 proteins, among which few protein...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-31140-y
更新日期:2018-08-28 00:00:00
abstract::We previously found that Homeobox containing 1 (HMBOX1) was required for bone mesenchymal stem cell (BMSC) and mouse embryonic stem cell (ESC) differentiation into vascular endothelial cells (VECs). However, the function of HMBOX1 in VECs is still unknown. In this study, we found that HMBOX1 was abundantly expressed i...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep15121
更新日期:2015-10-12 00:00:00
abstract::A number of studies have suggested that the irradiation behavior and damage processes occurring during sequential and simultaneous particle irradiations can significantly differ. Currently, there is no definite answer as to why and when such differences are seen. Additionally, the conventional multi-particle irradiati...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep30191
更新日期:2016-07-28 00:00:00
abstract::Exposure to silica can cause lung fibrosis and cancer. Identification of molecular targets is important for the intervention and/or prevention of silica-induced lung diseases. Telomeres consist of tandem repeats of DNA sequences at the end of chromosomes, preventing chromosomal fusion and degradation. Regulator of tel...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-17645-y
更新日期:2017-12-11 00:00:00
abstract::The treatment of testicular cancer includes unilateral orchiectomy and chemotherapy and is curative for most patients. However, observational studies revealed an association with depression, anxiety and cognitive impairment. It is unclear whether these side effects are caused by chemotherapy, hemicastration or the dis...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-67081-8
更新日期:2020-06-23 00:00:00
abstract::Since the advent of graphene, a variety of studies have been performed to elucidate its fundamental physics, or to explore its practical applications. Gate-tunable resistance is one of the most important properties of graphene and has been studied in 1-3 layer graphene in a number of efforts to control the band gap to...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-32214-7
更新日期:2018-09-18 00:00:00
abstract::mRNAs containing premature termination codons (PTCs) are rapidly degraded through nonsense-mediated mRNA decay (NMD). However, some PTC-containing mRNAs evade NMD, and might generate mutant proteins responsible for various diseases, including cancers. Using PTC-containing human genomic β-globin constructs, we show tha...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-16177-9
更新日期:2017-11-20 00:00:00
abstract::Sediment accretion (burial) and nutrient enrichment occur concurrently in lacustrine wetlands, but the role of these two aspects of sedimentation on macrophyte performance has rarely been examined. Here, we investigated the concurrent effects of sediment accretion and nutrient enrichment on the growth and propagation ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep39963
更新日期:2017-01-05 00:00:00