Abstract:
:Enhanced and immediate expression of cyclooxygenase-2 (COX-2) mRNA is observed in IL-1β-stimulated OA chondrocytes but the synthesis of protein found significantly delayed. Here we investigated the role of stress granules (SGs), ribonucleoprotein complexes that regulate mRNA translation, in the delayed translation of COX-2 mRNAs in IL-1β-stimulated OA chondrocytes. Stimulation of human chondrocytes with IL-1β activated the stress response genes and the phosphorylation of eIF2α that triggered the assembly of SGs. Using combined immunofluorescence staining of SGs markers and COX-2 protein, RNA fluorescence in situ hybridization and RNA immunoprecipitation, the COX-2 mRNAs were found sequestered in SGs in IL-1β-stimulated OA chondrocytes. No increase in COX-2 protein expression was observed during the persistence of SGs but enhanced expression of COX-2 protein was noted upon clearance of the SGs. Inhibition of SGs clearance blocked COX-2 mRNA translation whereas blocking the assembly of SGs by TIA-1 depletion resulted in rapid and increased production of COX-2 and PGE2. Our findings show for the first time assembly of SGs and sequestration of COX-2 mRNAs in human OA chondrocytes under pathological conditions. Post-transcriptional regulation of COX-2 mRNAs translation by SGs indicates a role in IL-1β-mediated catabolic response that could be therapeutically targeted in OA.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ansari MY,Haqqi TMdoi
10.1038/srep27611subject
Has Abstractpub_date
2016-06-08 00:00:00pages
27611issn
2045-2322pii
srep27611journal_volume
6pub_type
杂志文章abstract::Using 137Cs and 210Pb dating and multi-proxy evidence from a 41-cm sediment core from Bosten Lake in China, the responses of sediment grain size to environmental changes were reconstructed over the past 150 years. After the end of the Little Ice Age, the climate of the Bosten Lake region became warmer and drier, and t...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-57921-y
更新日期:2020-01-22 00:00:00
abstract::PROspective MOtion correction (PROMO) can prevent motion artefacts. The aim of this study was to determine whether brain structure measurements of motion-corrected images with PROMO were reliable and equivalent to conventional images without motion artefacts. The following T1-weighted images were obtained in healthy s...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep38366
更新日期:2016-12-05 00:00:00
abstract::Plant genomes encode several classes of small regulatory RNAs (sRNAs) that play critical roles in both development and stress responses. Panax notoginseng (Burk.) F.H. Chen (P. notoginseng) is an important traditional Chinese herbal medicinal plant species for its haemostatic effects. Therefore, the root yield of P. n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-09670-8
更新日期:2017-08-25 00:00:00
abstract::Thermoelectric effects in magnetic tunnel junctions are promising to serve as the basis for logic devices or memories in a "green" information technology. However, up to now the readout contrast achieved with Seebeck effects was magnitudes smaller compared to the well-established tunnel magnetoresistance effect. Here,...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08945
更新日期:2015-03-10 00:00:00
abstract::In bacteria, selective promoter recognition by RNA polymerase is achieved by its association with σ factors, accessory subunits able to direct RNA polymerase "core enzyme" (E) to different promoter sequences. Using Chromatin Immunoprecipitation-sequencing (ChIP-seq), we searched for promoters bound by the σ(S)-associa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep10469
更新日期:2015-05-28 00:00:00
abstract::This study was carried out to use hyperspectral imaging technique for determining color (L*, a* and b*) and eggshell strength and identifying cracked chicken eggs. Partial least squares (PLS) models based on full and selected wavelengths suggested by regression coefficient (RC) method were established to predict the f...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep21130
更新日期:2016-02-17 00:00:00
abstract::Coenzyme engineering that changes NAD(P) selectivity of redox enzymes is an important tool in metabolic engineering, synthetic biology, and biocatalysis. Here we developed a high throughput screening method to identify mutants of 6-phosphogluconate dehydrogenase (6PGDH) from a thermophilic bacterium Moorella thermoace...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32644
更新日期:2016-09-02 00:00:00
abstract::The study of cancer immunology has provided diagnostic and therapeutic instruments through serum autoantibody biomarkers and exogenous monoclonal antibodies. While some endogenous antibodies are found within or surrounding transformed tissue, the extent to which this exists has not been entirely characterized. We find...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep05088
更新日期:2014-05-30 00:00:00
abstract::Marine bacterial strains are of great interest for their ability to produce secondary metabolites with anticancer potentials. Isolation, identification, characterization and anticancer activities of isolated bacteria from El-Hamra Lake, Wadi El-Natrun (Egypt) were the objectives of this study. The isolated bacteria we...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-70945-8
更新日期:2020-08-21 00:00:00
abstract::The role of miRNAs in mediating insecticide resistance remains largely unknown, even for the model species Drosophila melanogaster. Building on prior research, this study used microinjection of synthetic miR-310s mimics into DDT-resistant 91-R flies and observed both a significant transcriptional repression of computa...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-71250-0
更新日期:2020-09-01 00:00:00
abstract::Hedgehog interacting protein (Hhip) is essential for islet formation and beta-cell proliferation during pancreatic development; abnormally elevated Hhip expression has been linked to human pancreatitis. Here, we investigate the role of Hhip in modulating insulin secretion in adult Hhip mice (Hhip +/- vs. Hhip+/+) fed ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-47633-3
更新日期:2019-08-01 00:00:00
abstract::Joubert syndrome (JS) is an inherited ciliopathy characterized by a distinctive cerebellar and brain stem malformation which is known as the "molar tooth sign" on axial brain images, hypotonia, and developmental delay. Approximately 25-30% of patients with JS have kidney disease and many of them progress to end-stage ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-020-80712-4
更新日期:2021-01-11 00:00:00
abstract::The irritant receptor TRPA1 was suggested to mediate analgesic, antipyretic but also pro-inflammatory effects of the non-opioid analgesic acetaminophen, presumably due to channel activation by the reactive metabolites parabenzoquinone (pBQ) and N-acetyl-parabenzoquinonimine (NAPQI). Here we explored the effects of the...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-017-13054-3
更新日期:2017-10-06 00:00:00
abstract::Salvia miltiorrhiza Bunge, which contains tanshinones and phenolic acids as major classes of bioactive components, is one of the most widely used herbs in traditional Chinese medicine. Production of tanshinones and phenolic acids is enhanced by methyl jasmonate (MeJA). Transcription factor MYC2 is the switch of jasmon...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep22852
更新日期:2016-03-07 00:00:00
abstract::Discrimination of species and geographical origins of traditional Chinese medicine (TCM) is essential to prevent adulteration and inferior problems. We studied Ephedra sinica Stapf, Ephedra intermedia Schrenk et C.A.Mey. and Ephedra przewalskii Bge. to investigate the relationship between inorganic element content and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-28558-9
更新日期:2018-07-06 00:00:00
abstract::NOTCH plays a pivotal role during normal development and in congenital disorders and cancer. γ-secretase inhibitors are commonly used to probe NOTCH function, but also block processing of numerous other proteins. We discovered a new class of small molecule inhibitor that disrupts the interaction between NOTCH and RBPJ...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-46948-5
更新日期:2019-07-25 00:00:00
abstract::In this paper we report a low complexity compression method that is suitable for compact optical scanning holography (OSH) systems with different optical settings. Our proposed method can be divided into 2 major parts. First, an automatic decision maker is applied to select the rows of holographic pixels to be scanned...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep34724
更新日期:2016-10-06 00:00:00
abstract::Interfacial Dzyaloshinskii-Moriya interaction (IDMI) is important for its roles in stabilizing the skyrmionic lattice as well as soliton-like domain wall motion leading towards new generation spintronic devices. However, achievement and detection of IDMI is often hindered by various spurious effects. Here, we demonstr...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep32592
更新日期:2016-09-02 00:00:00
abstract::Myopia is the commonest eye disorder in the world. High myopes are predisposed to ocular pathologies. The vasoactive intestinal peptide receptor 2 (VIPR2) gene was identified as a myopia susceptibility locus by our group and another group. We continued to fine-map this locus. A case-control study was performed in 4 se...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-54619-8
更新日期:2019-12-03 00:00:00
abstract::A novel polyphenolic natural product, galewone, with undescribed carbon skeleton, was isolated as a racemate from the culture of Daldinia eschscholzii IFB-TL01, a fungus obtained from the mantis (Tenodera aridifolia) gut. The galewone structure was elucidated by a combination of MS and NMR spectra, and substantiated b...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-50868-9
更新日期:2019-10-04 00:00:00
abstract::Carbonic anhydrase plays a key role in CO2 transport, acid-base and ion regulation and metabolic processes in vertebrates. While several carbonic anhydrase isoforms have been identified in numerous vertebrate species, basal lineages such as the cyclostomes have remained largely unexamined. Here we investigate the repe...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep33954
更新日期:2016-10-05 00:00:00
abstract::Soils - constituting the largest terrestrial carbon pool - are vulnerable to climatic warming. Currently existing uncertainties regarding carbon fluxes within terrestrial systems can be addressed by studies of past carbon cycle dynamics and related climate change recorded in sedimentary successions. Here we show an ex...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep31930
更新日期:2016-08-24 00:00:00
abstract::Atomic hydrogen exists ubiquitously in graphene materials made by chemical methods. Yet determining the effect of hydrogen on the electrochemical performance of graphene remains a significant challenge. Here we report the experimental observations of high rate capacity in hydrogen-treated 3-dimensional (3D) graphene n...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep16190
更新日期:2015-11-05 00:00:00
abstract::Glutamate is an indispensable neurotransmitter, triggering postsynaptic signals upon recognition by postsynaptic receptors. We questioned the phylogenetic position and the molecular details of when and where glutamate recognition arose in the glutamate-gated chloride channels. Experiments revealed that glutamate recog...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep08558
更新日期:2015-02-24 00:00:00
abstract::Macular edema due to neovascular age-related macular degeneration, diabetes or retinal vein occlusion can cause central vision loss. Intravitreal treatment with antibody-based biopharmaceutical compounds designed to neutralize vascular endothelial growth factor (VEGF) has proven to be an efficient strategy to ameliora...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-20525-8
更新日期:2018-02-01 00:00:00
abstract::To tailor the properties of colloidal materials, precise control over the self-assembly of their constituents is a prerequisite. Here, we govern the assembly of silica particles by functionalization with supramolecular moieties which interact with each other via directional and reversible hydrogen bonding. Through a g...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-19282-5
更新日期:2018-01-19 00:00:00
abstract::We examined whether it is possible to identify individual subjects on the basis of brain anatomical features. For this, we analyzed a dataset comprising 191 subjects who were scanned three times over a period of two years. Based on FreeSurfer routines, we generated three datasets covering 148 anatomical regions (corti...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-018-23696-6
更新日期:2018-04-04 00:00:00
abstract::Turner syndrome (TS) is characterized by the partial or complete loss of one sex chromosome and results in growth failure, gonadal insufficiency and cardiac anomalies. Treatment with growth hormone (GH) during childhood has indisputable benefits when taking into account the low stature of TS women. Medical records and...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/s41598-019-52332-0
更新日期:2019-11-04 00:00:00
abstract::Exploiting pattern formation - such as that observed in nature - in the context of micro/nanotechnology could have great benefits if coupled with the traditional top-down lithographic approach. Here, we demonstrate an original and simple method to produce unique, localized and controllable self-organised patterns on e...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep14787
更新日期:2015-10-06 00:00:00
abstract::Aminoacyl-tRNA synthetases are the key enzymes for protein synthesis. Glycine, alanine, serine and tyrosine are the major amino acids composing fibroin of silkworm. Among them, the genes of alanyl-tRNA synthetase (AlaRS) and glycyl-tRNA synthetase (GlyRS) have been cloned. In this study, the seryl-tRNA synthetase (Ser...
journal_title:Scientific reports
pub_type: 杂志文章
doi:10.1038/srep41563
更新日期:2017-01-30 00:00:00