Abstract：
:The development of topical microbicides is a valid approach to protect the genital mucosa from sexually transmitted infections that cannot be contained with effective vaccination, like HSV and HIV infections. A suitable target of microbicides is the interaction between viral proteins and cell surface heparan sulfate proteoglycans (HSPGs). AGMA1 is a prevailingly cationic agmatine-containing polyamidoamine polymer previously shown to inhibit HSPGs dependent viruses, including HSV-1, HSV-2, and HPV-16. The aim of this study was to elucidate the mechanism of action of AGMA1 against HSV infection and assess its antiviral efficacy and biocompatibility in preclinical models. The results show AGMA1 to be a non-toxic inhibitor of HSV infectivity in cell cultures and human cervicovaginal histocultures. Moreover, it significantly reduced the burden of infection of HSV-2 genital infection in mice. The investigation of the mechanism of action revealed that AGMA1 reduces cells susceptibility to virus infection by binding to cell surface HSPGs thereby preventing HSV attachment. This study indicates that AGMA1 is a promising candidate for the development of a topical microbicide to prevent sexually transmitted HSV infections.
journal_name
Biomaterialsjournal_title
Biomaterialsauthors
Donalisio M,Quaranta P,Chiuppesi F,Pistello M,Cagno V,Cavalli R,Volante M,Bugatti A,Rusnati M,Ranucci E,Ferruti P,Lembo Ddoi
10.1016/j.biomaterials.2016.01.055subject
Has Abstractpub_date
2016-04-01 00:00:00pages
40-53eissn
0142-9612issn
1878-5905pii
S0142-9612(16)00075-2journal_volume
85pub_type
杂志文章相关文献
BIOMATERIALS文献大全abstract：:The histology and cytology of the tissue response to intra-articular insertion of particles of acrylic bone cement containing barium sulphate has been studied in the guinea pig knee joint. Following insertion of the acrylic, fibrinous debris admixed with particles became adherent to the synovial membrane. Cell prolife...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(84)90003-6
更新日期：1984-03-01 00:00:00
abstract：:We demonstrate the ability of immobilized vascular endothelial growth factor (VEGF) to capture endothelial cells (EC) with high specificity under fluid flow. To this end, we engineered a surface consisting of heparin bound to poly-l-lysine to permit immobilization of VEGF through the C-terminal heparin-binding domain....
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.02.025
更新日期：2015-05-01 00:00:00
abstract：:Photodynamic therapy (PDT) induced hypoxia can significantly upregulate the expression of vascular endothelial growth factor (VEGF) at the tumor-stromal interface, resulting in a promoted angiogenesis. Thus, an angiogenesis vessel-targeting nanoparticle (AVT-NP) consisting of photosensitizer, angiogenic vessel-targeti...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2017.08.032
更新日期：2017-11-01 00:00:00
abstract：:Translation of micelles from the laboratory to the clinic is limited by a poor understanding of their in vivo fate following administration. In this paper, we establish a robust approach to real-time monitoring of the in vivo stability of micelles using Förster Resonance Energy Transfer (FRET). This characterization m...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2014.01.027
更新日期：2014-04-01 00:00:00
abstract：:The aim of this study, in rabbit tibia, was an evaluation of the early reactions of the tissues to the insertion of polylactic membranes, used in connection with titanium implants. The specimens were retrieved after 1-4 weeks, and a histological analysis was performed. It was possible to see that, in the early implant...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(97)00173-7
更新日期：1998-05-01 00:00:00
abstract：:The elasticity, mechanical strength and permeability of a new biomaterial made of elastin and fibrin were investigated. It was shown that gamma-irradiation to sterilize the product, and sulphur derivatives (Merceptyl, thiourea, cystein) working as reticulating agents modified these properties. Depending on whether or ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(89)90084-7
更新日期：1989-09-01 00:00:00
abstract：:Platelet-derived growth factor BB (PDGF-BB) was an important inductive factor during wound healing, but the lack of efficient delivery system limited its clinical application. Here, a peptide of seven amino acids was firstly utilized to engineer PDGF-BB to construct a collagen-targeting system. This peptide functioned...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.07.023
更新日期：2006-11-01 00:00:00
abstract：:Colloidal nanoparticle drug delivery systems have attracted much interest for their ability to enable effective formulation and delivery of therapeutic agents. The selective delivery of these nanoparticles to the disease site can be enhanced by coating the surface of the nanoparticles with targeting moieties, such as ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.07.065
更新日期：2011-11-01 00:00:00
abstract：:Combined treatment using nonviral agent-mediated enzyme/prodrug therapy and immunotherapy had been proposed as a powerful alternative method of cancer therapy. The present study was aimed to evaluate the cytotoxicity in vitro and the therapeutic efficacy in vivo when the cytosine deaminase/5-fluorocytosine (CD/5-FC) a...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2009.04.011
更新日期：2009-08-01 00:00:00
abstract：:The phosphoinositide 3-kinase/Akt1 signaling pathway has emerged as a target for cancer therapy. In this study, we aimed to develop a strategy to enhance Akt-targeted cancer therapy. We hypothesized that combination of Akt1-targeted therapy with conventional chemotherapy using paclitaxel-incorporated conjugated linole...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.12.011
更新日期：2012-03-01 00:00:00
abstract：:Complement activation by polymeric gene and drug delivery systems has been overlooked in the past. As more reports appear in the literature concerning immunogenicity of polymers and their impact on gene expression patterns, it is important to address possible immune side effects of polymers, namely complement activati...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.03.035
更新日期：2011-07-01 00:00:00
abstract：:Bone tissue engineering strategies are fundamentally based upon porous scaffold materials that serve as a support for ingrowth of host cells and/or provide a substrate for exogenously delivered cells. Here we report the application of a surface calcium phosphate (CaP) mineral layer to a macroporous polymeric/CaP compo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2006.11.025
更新日期：2007-03-01 00:00:00
abstract：:Percutaneous implants are commonly associated with several problems, and different failure modes have been described. Infections constitute one serious complication which may lead to the removal of the implant. In contrast to infections around polymer implants, infections around skin-penetrating titanium implants anch...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(99)00033-2
更新日期：1999-07-01 00:00:00
abstract：:Bioactive composites composed of hydroxyapatite containing glass (HA-G) as a coating and titanium (Ti) or titanium alloy implants as a substrate were prepared by the Cullet method. This method results in the HA-G coating layer on the substrate with a compositional gradient in HA concentration. The results of in vitro ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(00)00402-6
更新日期：2001-08-01 00:00:00
abstract：:Cytoxicity is an essential concern for polyethyleneimine 25 kDa (PEI 25 kDa), a widely reported, highly effective transfection agent used in gene delivery. In our recent experiments, Small molecular weight cross-linked poly(ethylene imine) by biscarbamate linkage (PEI-Bu) (Mn: 3278, Mw: 4289) can reduce target cell ap...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.05.044
更新日期：2012-09-01 00:00:00
abstract：:Therapeutic options to treat primary glioblastoma (GBM) tumors are scarce. GBM tumors with epidermal growth factor receptor (EGFR) mutations, in particular a constitutively active EGFRvIII mutant, have extremely poor clinical outcomes. GBM tumors with concurrent EGFR amplification and active phosphatase and tensin hom...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.119371
更新日期：2019-10-01 00:00:00
abstract：:Hierarchical structured nanomaterials with diverse functionality, such as magnetic susceptibility, stimuli-responsiveness, environmental sensing and biocompatibility, are highly sought after for biomedicine and biodetection alike. In this study, we designed and fabricated a new kind of multifunctional core/shell nanos...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.06.046
更新日期：2013-10-01 00:00:00
abstract：:Glioblastoma multiforme (GBM) is among the most aggressive cancers associated with massive infiltration of peritumoral parenchyma by migrating tumor cells. The infiltrative nature of GBM cells, the intratumoral heterogeneity concomitant with redundant signaling pathways likely underlie the inability of conventional an...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2016.08.009
更新日期：2016-11-01 00:00:00
abstract：:Current prosthetic small diameter vascular grafts show poor long-term patency rates, leading to the pursuit of a biological alternative. Hyaff-11 is a hyaluronan-based biodegradable polymer developed for tissue-engineering applications. This study aimed to determine whether human vascular endothelial cells attach to H...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2004.02.002
更新日期：2004-12-01 00:00:00
abstract：:Although photothermal therapy (PTT) and photodynamic therapy (PDT) are widely commended for tumor treatment recently, they still suffer severe challenges due to the non-specificity of photothermal agents (PTAs)/photosensitizers (PSs) and hypoxic tumor microenvironment. Here, an oxygen independent biomimetic nanoplatfo...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2019.01.041
更新日期：2019-04-01 00:00:00
abstract：:This study was undertaken to evaluate the influence of storage conditions on the shelf-life of porcine bioprosthetic valves. Fifty-five unimplanted porcine bioprostheses have been evaluated. The valves were stored in 0.5% buffered glutaraldehyde solution for different periods of time (7, 23 and 32 months). Twenty-eigh...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/s0142-9612(96)00155-x
更新日期：1997-04-01 00:00:00
abstract：:Xenogeneic extracellular matrix (ECM) can be harvested and configured to function as a bioscaffold for tissue and organ reconstruction. The mechanical properties of the ECM vary depending upon the tissue from which it is harvested. Likewise, the manufacturing steps required to develop ECMs into medical grade devices w...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2003.09.015
更新日期：2004-05-01 00:00:00
abstract：:A homogeneous glycidyl acrylate polymer (GAP) has been grafted on to polytetrafluoroethylene (PTFE) and polyethylene (PE) using a modified plasma glow discharge technique with glycidyl acrylate. The polymeric layer appears to be extremely stable to acidic media and to common organic solvents. The modified surface can ...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(90)90083-3
更新日期：1990-10-01 00:00:00
abstract：:There is a broad interest in the development of nanoparticles (NPs) carrying on their surface carbohydrates such as sialic acids. It is known that these carbohydrates influence the biological and physical properties of biopharmaceutical proteins and living cells. Macromolecular compounds containing these carbohydrates...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2010.01.049
更新日期：2010-04-01 00:00:00
abstract：:Hematopoietic stem cells (HSCs) are the vital, life-long source of all blood cell types. They are found in stem cell niches, specific anatomic locations that offer all the factors and signals necessary for the maintenance of the stem cell potential of HSCs. Much attention has been paid to the biochemical composition o...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2012.01.002
更新日期：2012-04-01 00:00:00
abstract：:High throughput cellular studies require small sample volume to reduce costs and enhance sensitivity. Microfluidics-generated water-in-oil (W/O) single emulsion droplet systems, in particular, provide uniform, well defined and discrete microenvironment for cell culture, screening, and sorting. However, these single em...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2013.03.002
更新日期：2013-06-01 00:00:00
abstract：:The bulk, surface and blood-contacting properties of a series of polyether polyurethanes, modified with three different polydimethylsiloxane (PDMS) macroglycol segments, were evaluated. The PDMS oligomers were terminated with hydroxy-tipped end groups of varying polarity. The effect of substituting the polytetramethyl...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/0142-9612(89)90005-7
更新日期：1989-01-01 00:00:00
abstract：:To investigate the role that the micro/nano-environment plays on the differentiation pathway of bone marrow stromal cells (BMSCs) into osteoblasts, we employed a 2D substrate coated with turnip yellow mosaic virus (TYMV) particles. TYMV is a non-enveloped icosahedral plant virus which has an average diameter 28 nm and...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2008.06.029
更新日期：2008-10-01 00:00:00
abstract：:Micro/nanotopographical modification of biomaterials constitutes a promising approach to direct stem cell osteogenic differentiation to promote osseointegration. In this work, titania nanotubes (NTs) 25 and 80 nm in size with the acid-etched Ti topography (AcidTi) and hierarchical hybrid micropitted/nanotubular topogr...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2011.12.024
更新日期：2012-03-01 00:00:00
abstract：:Magnesium (Mg), a potential biodegradable material, has recently received increasing attention due to its unique antibacterial property. However, rapid corrosion in the physiological environment and potential toxicity limit clinical applications. In order to improve the corrosion resistance meanwhile not compromise th...
journal_title：Biomaterials
pub_type： 杂志文章
doi：10.1016/j.biomaterials.2015.02.096
更新日期：2015-06-01 00:00:00
