The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus.

Abstract:

:To investigate the role that the micro/nano-environment plays on the differentiation pathway of bone marrow stromal cells (BMSCs) into osteoblasts, we employed a 2D substrate coated with turnip yellow mosaic virus (TYMV) particles. TYMV is a non-enveloped icosahedral plant virus which has an average diameter 28 nm and the protein cage structure consists of 180 identical subunits. The temporal effect of TYMV coated substrate on the adhesion and differentiation capacity of the BMSCs was monitored for selected time periods of 7, 14 and 21 days. We examined the gene expression profile of BMSCs cultured in primary media (undifferentiated cells) and cells induced to osteoblast lineage by real time PCR analysis. To further corroborate our findings, we investigated the expression of osteogenic markers using immunohistochemistry and cytochemical staining. As expected, the genes involved in the process of osteogenic differentiation were activated more during the growth of cells under osteogenic media. In addition, we found that the BMSCs induced to undergo osteogenic differentiation on TYMV coated substrates formed fully mineralized nodules comprising of osteoblast-like cells around day 14. Comparing the gene expression pattern of BMSCs induced to osteogenic differentiation under standard culture conditions with the cells induced on TYMV substrates, we found significant differences in the temporal expression and level of expression of several key genes. Our findings indicate that TYMV, as a biogenic nanoparticle, can be employed as a model to modulate the nano-environment of the substrates in order to gain an insight into the role that the micro/nano-environment has in regulating adhesion, growth and differentiation of BMSCs towards osteogenic lineage, which will be vital for designing compatible biomaterials for tissue engineering purposes.

journal_name

Biomaterials

journal_title

Biomaterials

authors

Kaur G,Valarmathi MT,Potts JD,Wang Q

doi

10.1016/j.biomaterials.2008.06.029

subject

Has Abstract

pub_date

2008-10-01 00:00:00

pages

4074-81

issue

30

eissn

0142-9612

issn

1878-5905

pii

S0142-9612(08)00448-1

journal_volume

29

pub_type

杂志文章
  • The cell penetrating ability of the proapoptotic peptide, KLAKLAKKLAKLAK fused to the N-terminal protein transduction domain of translationally controlled tumor protein, MIIYRDLISH.

    abstract::We show here, that the proapoptotic peptide, KLAKLAKKLAKLAK (KLA), which by itself does not penetrate cell membranes, can do so when fused to a protein transduction domain derived from NH(2)-terminus of translationally controlled tumor protein (TCTP-PTD, MIIYRDLISH). Once inside the cell, the conjugated KLA exerts its...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.03.074

    authors: Kim HY,Kim S,Youn H,Chung JK,Shin DH,Lee K

    更新日期:2011-08-01 00:00:00

  • Selective local delivery of RANK siRNA to bone phagocytes using bone augmentation biomaterials.

    abstract::Fracture healing and fracture fixation in the context of osteoporosis is extremely difficult. To inhibit osteoclast-induced bone resorption and associated implant loosening in this pathology, we describe a local delivery strategy to delivery RNA interfering technology to bone sites to target and down-regulate osteocla...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2012.07.039

    authors: Wang Y,Tran KK,Shen H,Grainger DW

    更新日期:2012-11-01 00:00:00

  • When stem cells meet graphene: Opportunities and challenges in regenerative medicine.

    abstract::Recent advances in stem cell research and nanotechnology have significantly influenced the landscape of tissue engineering and regenerative medicine. Precise and reproducible control of the fate of stem cells and their lineage specification have, therefore, become more crucial than ever for the success of stem cell-ba...

    journal_title:Biomaterials

    pub_type: 杂志文章,评审

    doi:10.1016/j.biomaterials.2017.10.004

    authors: Kenry,Lee WC,Loh KP,Lim CT

    更新日期:2018-02-01 00:00:00

  • Migration of lymphocytes on fibronectin-coated surfaces: temporal evolution of migratory parameters.

    abstract::Lymphocytes typically interact with implanted biomaterials through adsorbed exogenous proteins. To provide a more complete characterization of these interactions, analysis of lymphocyte migration on adsorbed extracellular matrix proteins must accompany the commonly performed adhesion studies. We report here a comparis...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(99)00154-4

    authors: Bergman AJ,Zygourakis K

    更新日期:1999-12-01 00:00:00

  • Engineering retinal progenitor cell and scrollable poly(glycerol-sebacate) composites for expansion and subretinal transplantation.

    abstract::Retinal degenerations cause permanent visual loss and affect millions world-wide. Presently, a novel treatment highlights the potential of using biodegradable polymer scaffolds to induce differentiation and deliver retinal progenitor cells for cell replacement therapy. In this study, we engineered and analyzed a micro...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.02.046

    authors: Redenti S,Neeley WL,Rompani S,Saigal S,Yang J,Klassen H,Langer R,Young MJ

    更新日期:2009-07-01 00:00:00

  • Genetically encoded FRET-based biosensor for imaging MMP-9 activity.

    abstract::A genetically encoded Förster Resonance Energy Transfer (FRET)-based biosensor that continuously monitors matrix metalloproteinase 9 (MMP-9) activity was developed. MMP-9 is an extracellularly acting endopeptidase with a prominent role in development, learning and memory, cancer metastasis, and stroke. To assess the b...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2013.11.033

    authors: Stawarski M,Rutkowska-Wlodarczyk I,Zeug A,Bijata M,Madej H,Kaczmarek L,Wlodarczyk J

    更新日期:2014-02-01 00:00:00

  • A mixture model for water uptake, degradation, erosion and drug release from polydisperse polymeric networks.

    abstract::We introduce a general class of mixture models suitable to describe water-dependent degradation and erosion of biodegradable polymers in conjunction with drug release. The ability to predict and quantify degradation and erosion has direct impact in a variety of biomedical applications and is a useful design tool for b...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.01.008

    authors: Soares JS,Zunino P

    更新日期:2010-04-01 00:00:00

  • Dragon fruit-like biocage as an iron trapping nanoplatform for high efficiency targeted cancer multimodality imaging.

    abstract::Natural biopolymer based multifunctional nanomaterials are perfect candidates for multimodality imaging and therapeutic applications. Conventional methods of building multimodal imaging probe require either cross-linking manners to increase its in vivo stability or attach a target module to realize targeted imaging. I...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2015.08.001

    authors: Yang M,Fan Q,Zhang R,Cheng K,Yan J,Pan D,Ma X,Lu A,Cheng Z

    更新日期:2015-11-01 00:00:00

  • The potential of chitosan-based gels containing intervertebral disc cells for nucleus pulposus supplementation.

    abstract::The suitability of chitosan-based hydrogels as scaffolds for the encapsulation of intervertebral disc (IVD) cells and the accumulation of a functional extracellular matrix mimicking that of the nucleus pulposus (NP) was investigated. The specific hypothesis under study was that the cationic chitosan would form an idea...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2005.06.037

    authors: Roughley P,Hoemann C,DesRosiers E,Mwale F,Antoniou J,Alini M

    更新日期:2006-01-01 00:00:00

  • Autonomous assembly of ordered metastable DNA nanoarchitecture and in situ visualizing of intracellular microRNAs.

    abstract::Facile assembly of intelligent DNA nanoobjects with the ability to exert in situ visualization of intracellular microRNAs (miRNAs) has long been concerned in the fields of DNA nanotechnology and basic medical study. Here, we present a driving primer (DP)-triggered polymerization-mediated metastable assembly (PMA) stra...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2016.12.018

    authors: Xu J,Wu ZS,Wang Z,Le J,Zheng T,Jia L

    更新日期:2017-03-01 00:00:00

  • The role of cell-released microvesicles in the intercellular transfer of magnetic nanoparticles in the monocyte/macrophage system.

    abstract::Mononuclear phagocyte system is the first target for injected nanomaterials. Monocytes/macrophages are the central actors for trafficking and clearance of magnetic nanoparticles in vivo. However the fate of nanosized magnetic label is an ongoing issue. Here we demonstrate that the monocyte/macrophage system shows a co...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.05.062

    authors: Luciani N,Wilhelm C,Gazeau F

    更新日期:2010-09-01 00:00:00

  • cRGD-functionalized, DOX-conjugated, and ⁶⁴Cu-labeled superparamagnetic iron oxide nanoparticles for targeted anticancer drug delivery and PET/MR imaging.

    abstract::Multifunctional and water-soluble superparamagnetic iron oxide (SPIO) nanocarriers were developed for targeted drug delivery and positron emission tomography/magnetic resonance imaging (PET/MRI) dual-modality imaging of tumors with integrin α(v)β₃ expression. An anticancer drug was conjugated onto the PEGylated SPIO n...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.02.006

    authors: Yang X,Hong H,Grailer JJ,Rowland IJ,Javadi A,Hurley SA,Xiao Y,Yang Y,Zhang Y,Nickles RJ,Cai W,Steeber DA,Gong S

    更新日期:2011-06-01 00:00:00

  • Enhancement of ectopic osteoid formation following the dual release of bone morphogenetic protein 2 and Wnt1 inducible signaling pathway protein 1 from gelatin sponges.

    abstract::Bone morphogenetic protein (BMP) 2-incorporated gelatin sponge is effective for in vivo osteoinduction. However, the modeling capacity of bone decreases with age. As atrial to stimulate effective bone formation for animals with decreased osteogenic potential, Wnt1 inducible signaling pathway protein (WISP) 1, an osteo...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2011.04.035

    authors: Kohara H,Tabata Y

    更新日期:2011-08-01 00:00:00

  • Osteoblast response to PLGA tissue engineering scaffolds with PEO modified surface chemistries and demonstration of patterned cell response.

    abstract::Because tissues are characterized by a well-defined three-dimensional arrangement of cells, tissue engineering scaffolds that facilitate the organization and differentiation of new tissue will have improved performance in comparison to scaffolds that only provide surfaces for cell attachment and growth. We hypothesize...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2003.09.064

    authors: Koegler WS,Griffith LG

    更新日期:2004-06-01 00:00:00

  • Silicon substitution in the calcium phosphate bioceramics.

    abstract::Silicon (Si) substitution in the crystal structures of calcium phosphate (CaP) ceramics such as hydroxyapatite (HA) and tricalcium phosphate (TCP) generates materials with superior biological performance to stoichiometric counterparts. Si, an essential trace element required for healthy bone and connective tissues, in...

    journal_title:Biomaterials

    pub_type: 杂志文章,评审

    doi:10.1016/j.biomaterials.2007.05.003

    authors: Pietak AM,Reid JW,Stott MJ,Sayer M

    更新日期:2007-10-01 00:00:00

  • The effect of micronscale anisotropic cross patterns on fibroblast migration.

    abstract::Cell movement on adhesive surfaces is a complicated process based on myriad cell-surface interactions. Although both micron and nanoscale surface topography have been known to be important in understanding cell-materials interactions, typically only simple patterns (e.g., parallel lines or aligned posts) have been use...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.01.103

    authors: Jeon H,Hidai H,Hwang DJ,Healy KE,Grigoropoulos CP

    更新日期:2010-05-01 00:00:00

  • Microspheres of hydroxyapatite/reconstituted collagen as supports for osteoblast cell growth.

    abstract::Microspheres comprised of particulate hydroxyapatites dispersed in fibrous collagen matrices were prepared. The procedure involved the droplet formation of hydroxyapatite/collagen mixture emulsified in olive oil, followed by the reconstitution of collagen in the presence of hydroxyapatite particles at 37 degrees C. Va...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(99)00095-2

    authors: Hsu FY,Chueh SC,Wang YJ

    更新日期:1999-10-01 00:00:00

  • Resorbable materials of poly(L-lactide). VI. Plates and screws for internal fracture fixation.

    abstract::Poly(L-lactide) (PLLA) with an extremely high molecular weight (Mv up to 1 X 10(6)) was synthesized at a low catalyst concentration (0.015 wt%) and temperatures between 100-110 degrees C. Besides good mechanical properties the as-polymerized PLLA exhibited a microporous structure. Plates and screws of this material we...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(87)90034-2

    authors: Leenslag JW,Pennings AJ,Bos RR,Rozema FR,Boering G

    更新日期:1987-01-01 00:00:00

  • Thermally responsive core-shell nanoparticles self-assembled from cholesteryl end-capped and grafted polyacrylamides:; drug incorporation and in vitro release.

    abstract::The thermally responsive cholesteryl end-capped poly(N-isopropylacrylamide-co-N,N-dimethylacrylamide) and cholesteryl grafted poly[N-isopropylacrylamide-co-N-(hydroxymethyl) acrylamide] amphiphilic polymers were synthesized and utilized to encapsulate cyclosporin A (CyA) and indomethacin (IND) within core-shell nanopa...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2003.10.095

    authors: Chaw CS,Chooi KW,Liu XM,Tan CW,Wang L,Yang YY

    更新日期:2004-08-01 00:00:00

  • Mechanical properties, drug eluting characteristics and in vivo performance of a genipin-crosslinked chitosan polymeric stent.

    abstract::A limitation with the use of polymers as stent matrices is their inherent mechanical weakness. In this study, a polymeric stent, made from chitosan-based films fixed by genipin which has a cyclic molecular structure, was developed (the genipin stent). The mechanical properties of the genipin stent were investigated; i...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2009.06.039

    authors: Chen MC,Liu CT,Tsai HW,Lai WY,Chang Y,Sung HW

    更新日期:2009-10-01 00:00:00

  • Carbon nanotube-based substrates for modulation of human pluripotent stem cell fate.

    abstract::We investigated the biological response of human pluripotent stem cells (hPSCs) cultured on a carbon nanotube (CNT) array-based substrate with the long term goal to direct hPSC germ layer specification for a wide variety of tissue engineering applications. CNT arrays were fabricated using a chemical vapor deposition s...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2014.03.011

    authors: Pryzhkova MV,Aria I,Cheng Q,Harris GM,Zan X,Gharib M,Jabbarzadeh E

    更新日期:2014-06-01 00:00:00

  • An airway smooth muscle cell niche under physiological pulsatile flow culture using a tubular dense collagen construct.

    abstract::Bioengineered tissue equivalents should provide physiologically relevant biochemical and mechanical cues to support the growth and differentiation of seeded cells. Herein, tubular dense collagen constructs (TDCCs) with collagen content comparable to native extracellular matrix were used to investigate the effect of sh...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2012.11.025

    authors: Ghezzi CE,Risse PA,Marelli B,Muja N,Barralet JE,Martin JG,Nazhat SN

    更新日期:2013-03-01 00:00:00

  • Investigation of the torque-tension characteristics of a flanged external fixator pin.

    abstract::The torque-tension characteristics of standard, conical and flanged external fixator pins and a cortical screw were compared using a commercially available testing apparatus. The flanged pin and the screw were similar in behaviour, and converted torque into tension more efficiently than conical and standard external f...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/0142-9612(95)98137-4

    authors: Oni OO,Batchelor JS

    更新日期:1995-11-01 00:00:00

  • Magnetic targeting of cardiosphere-derived stem cells with ferumoxytol nanoparticles for treating rats with myocardial infarction.

    abstract::Stem cell transplantation is a promising therapeutic strategy for acute or chronic ischemic cardiomyopathy. A major limitation to efficacy in cell transplantation is the low efficiency of retention and engraftment, due at least in part to significant early "wash-out" of cells from coronary blood flow and heart contrac...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2014.06.031

    authors: Vandergriff AC,Hensley TM,Henry ET,Shen D,Anthony S,Zhang J,Cheng K

    更新日期:2014-10-01 00:00:00

  • The glass ionomer cement: the sources of soluble fluoride.

    abstract::This study aimed to investigate certain processes of fluoride production which enable glass ionomer cements to leach fluoride. Two fluoroaluminosilicate glasses, G338 and LG26 were used. The free and total fluoride which could be dissolved from the glasses was measured, before and after acetic acid washing. Both glass...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(01)00352-0

    authors: Williams JA,Billington RW,Pearson GJ

    更新日期:2002-05-01 00:00:00

  • The significance of pore microarchitecture in a multi-layered elastomeric scaffold for contractile cardiac muscle constructs.

    abstract::Multi-layered poly(glycerol-sebacate) (PGS) scaffolds with controlled pore microarchitectures were fabricated, combined with heart cells, and cultured with perfusion to engineer contractile cardiac muscle constructs. First, one-layered (1L) scaffolds with accordion-like honeycomb shaped pores and elastomeric mechanica...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2010.11.032

    authors: Park H,Larson BL,Guillemette MD,Jain SR,Hua C,Engelmayr GC Jr,Freed LE

    更新日期:2011-03-01 00:00:00

  • The effect of hydroxyapatite coating on bony ingrowth into grooved titanium implants.

    abstract::This study was undertaken to investigate the relative importance of a hydroxyapatite (HA) coating and the macrotexture of titanium implants to the quality of bony ingrowth and fixation. Various types of titanium cylinders were implanted into the cancellous bone of the intercondylar region of the distal femur of the do...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(98)00011-8

    authors: Hayashi K,Mashima T,Uenoyama K

    更新日期:1999-01-01 00:00:00

  • Induction of protein oxidation by cobalt and chromium ions in human U937 macrophages.

    abstract::Metal particles and ions from hip prostheses have the potential to induce the production of reactive oxygen species (ROS), making them prime suspects for disturbing the cellular balance of oxidants/antioxidants (redox state of the cell). To better understand the cellular effect of metal ions from metal-on-metal prosth...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2004.11.019

    authors: Petit A,Mwale F,Tkaczyk C,Antoniou J,Zukor DJ,Huk OL

    更新日期:2005-07-01 00:00:00

  • Hydrogels based on poly(ethylene oxide) and poly(tetramethylene oxide) or poly(dimethyl siloxane): synthesis, characterization, in vitro protein adsorption and platelet adhesion.

    abstract::In vitro protein adsorption, platelet adhesion and activation on new hydrogel surfaces, composed of poly(ethylene oxide) (PEO) and poly(tetramethylene oxide) (PTMO) or poly(dimethyl siloxane) (PDMS), were investigated. By varying PEO length (MW = 2000 or 3400), hydrophobic components (PTMO or PDMS) or polymer topology...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/s0142-9612(01)00306-4

    authors: Park JH,Bae YH

    更新日期:2002-04-01 00:00:00

  • The transdermal inhibition of melanogenesis by a cell-membrane-permeable peptide delivery system based on poly-arginine.

    abstract::Topical therapy is the most favored form of treatment for whitening against hyper-pigmentation and sunburn because it lends itself to self-administration, patient compliance and an absence of systemic adverse effects. However, high-molecular-weight, hydrophilic chemicals are difficult to use as transdermal delivery dr...

    journal_title:Biomaterials

    pub_type: 杂志文章

    doi:10.1016/j.biomaterials.2014.01.052

    authors: Ookubo N,Michiue H,Kitamatsu M,Kamamura M,Nishiki T,Ohmori I,Matsui H

    更新日期:2014-05-01 00:00:00