当前位置： SCI文献检索 > Scientific Reports期刊下所有文献 > Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.

Scalable topographies to support proliferation and Oct4 expression by human induced pluripotent stem cells.

Abstract：

:It is well established that topographical features modulate cell behaviour, including cell morphology, proliferation and differentiation. To define the effects of topography on human induced pluripotent stem cells (iPSC), we plated cells on a topographical library containing over 1000 different features in medium lacking animal products (xeno-free). Using high content imaging, we determined the effect of each topography on cell proliferation and expression of the pluripotency marker Oct4 24 h after seeding. Features that maintained Oct4 expression also supported proliferation and cell-cell adhesion at 24 h, and by 4 days colonies of Oct4-positive, Sox2-positive cells had formed. Computational analysis revealed that small feature size was the most important determinant of pluripotency, followed by high wave number and high feature density. Using this information we correctly predicted whether any given topography within our library would support the pluripotent state at 24 h. This approach not only facilitates the design of substrates for optimal human iPSC expansion, but also, potentially, identification of topographies with other desirable characteristics, such as promoting differentiation.

journal_name

Sci Rep

journal_title

Scientific reports

authors

Reimer A,Vasilevich A,Hulshof F,Viswanathan P,van Blitterswijk CA,de Boer J,Watt FM

doi

10.1038/srep18948

subject

Has Abstract

pub_date

2016-01-13 00:00:00

pages

18948

issn

2045-2322

pii

srep18948

journal_volume

6

pub_type

杂志文章

相关文献

Scientific Reports文献大全
  • Impact of the severity of restrictive spirometric pattern on nutrition, physical activity, and quality of life: results from a nationally representative database.

    abstract：:The general disease burden associated with the restrictive spirometric pattern (RSP) is substantial. However, the impact of RSP by its severity on general health problems and quality of life has not been well elucidated. This study aimed to analyse nutrition, physical activity, and quality of life in subjects who part...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-76777-w

    authors： Chung SJ,Kim HI,Yang B,Kim T,Sim YS,Kang HK,Kim SH,Yoon HJ,Choi H,Lee H

    更新日期：2020-11-12 00:00:00

  • Wave manipulation with magnetically tunable metasurfaces.

    abstract：:Tunable metasurfaces have emerged as an efficient approach to manipulate the wave propagation. Different from previous work concentrating on electrically tunable mechanisms, here we demonstrate a magnetically tunable metasurface composed of ferrite rods and metallic foils. By tuning the thickness of ferrite rods, meta...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-05625-1

    authors： Yang H,Yu T,Wang Q,Lei M

    更新日期：2017-07-14 00:00:00

  • Novel HLA class I associations with HIV-1 control in a unique genetically admixed population.

    abstract：:Associations between HLA class I alleles and HIV progression in populations exhibiting Amerindian and Caucasian genetic admixture remain understudied. Using univariable and multivariable analyses we evaluated HLA associations with five HIV clinical parameters in 3,213 HIV clade B-infected, ART-naïve individuals from M...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-23849-7

    authors： Valenzuela-Ponce H,Alva-Hernández S,Garrido-Rodríguez D,Soto-Nava M,García-Téllez T,Escamilla-Gómez T,García-Morales C,Quiroz-Morales VS,Tapia-Trejo D,Del Arenal-Sánchez S,Prado-Galbarro FJ,Hernández-Juan R,Rodríguez-Aguirre E,

    更新日期：2018-04-17 00:00:00

  • Controlled mud-crack patterning and self-organized cracking of polydimethylsiloxane elastomer surfaces.

    abstract：:Exploiting pattern formation - such as that observed in nature - in the context of micro/nanotechnology could have great benefits if coupled with the traditional top-down lithographic approach. Here, we demonstrate an original and simple method to produce unique, localized and controllable self-organised patterns on e...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep14787

    authors： Seghir R,Arscott S

    更新日期：2015-10-06 00:00:00

  • Method for high frequency tracking and sub-nm sample stabilization in single molecule fluorescence microscopy.

    abstract：:While fluorescence microscopes and atomic force microscopes are widely used to visualize, track, and manipulate single biomolecules, the resolution of these methods is limited by sample drift. To minimize drift, active feedback methods have recently been used to stabilize single molecule microscopes on the sub-nanomet...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-32012-1

    authors： Schmidt PD,Reichert BH,Lajoie JG,Sivasankar S

    更新日期：2018-09-17 00:00:00

  • A 2-Cys peroxiredoxin in response to oxidative stress in the pine wood nematode, Bursaphelenchus xylophilus.

    abstract：:The pine wood nematode, Bursaphelenchus xylophilus, is the causal agent of pine wilt disease that has devastated pine forests in Asia. Parasitic nematodes are known to have evolved antioxidant stress responses that defend against host plant defenses. In this study, the infestation of whitebark pine, Pinus bungean, wit...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep27438

    authors： Li Z,Zhang Q,Zhou X

    更新日期：2016-06-07 00:00:00

  • Ti3+ in corundum traces crystal growth in a highly reduced magma.

    abstract：:Aggregates of corundum crystals with skeletal to hopper morphology occur in pyroclastic rocks erupted from Cretaceous basaltic volcanoes on Mt Carmel, N. Israel. The rapid growth of the crystals trapped volumes of the parental Al2O3-supersaturated melt; phenocrysts of tistarite (Ti2O3) in the trapped melts indicate cr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-79739-4

    authors： Oliveira B,Griffin WL,Gain SEM,Saunders M,Shaw J,Toledo V,Afonso JC,O'Reilly SY

    更新日期：2021-01-28 00:00:00

  • Concurrent targeting of BMI1 and CDK4/6 abrogates tumor growth in vitro and in vivo.

    abstract：:Despite recent advances in cancer management and therapy, resistance to cytotoxic medications remains a major clinical challenge; hence, combination-based anti-cancer treatment regimens are currently gaining momentum. PTC-209 reduced BMI1 protein expression, while palbociclib inhibited CDK4, Rb, and pRbSer795 protein ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-50140-0

    authors： Elango R,Vishnubalaji R,Manikandan M,Binhamdan SI,Siyal AA,Alshawakir YA,Alfayez M,Aldahmash A,Alajez NM

    更新日期：2019-09-23 00:00:00

  • Impact of student-induced disturbance on stream macroinvertebrates differs among habitat types.

    abstract：:Environmental impacts from ecotourism and outdoor recreation activities on terrestrial and aquatic ecosystems are well-reported in the literature, but less is known regarding the impacts of outdoor environmental education activities. Student activity during stream classes may cause substrate disruption and localized i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-38210-1

    authors： Bossley JP,Smiley PC Jr

    更新日期：2019-02-05 00:00:00

  • Impact of different cell penetrating peptides on the efficacy of antisense therapeutics for targeting intracellular pathogens.

    abstract：:There is a pressing need for novel and innovative therapeutic strategies to address infections caused by intracellular pathogens. Peptide nucleic acids (PNAs) present a novel method to target intracellular pathogens due to their unique mechanism of action and their ability to be conjugated to cell penetrating peptides...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep20832

    authors： Abushahba MF,Mohammad H,Thangamani S,Hussein AA,Seleem MN

    更新日期：2016-02-10 00:00:00

  • Whole-transcriptome analysis of UUO mouse model of renal fibrosis reveals new molecular players in kidney diseases.

    abstract：:Transcriptome analysis by RNA-seq technology allows novel insights into gene expression and regulatory networks in health and disease. To better understand the molecular basis of renal fibrosis, we performed RNA-seq analysis in the Unilateral Ureteric Obstruction (UUO) mouse model. We analysed sham operated, 2- and 8-...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep26235

    authors： Arvaniti E,Moulos P,Vakrakou A,Chatziantoniou C,Chadjichristos C,Kavvadas P,Charonis A,Politis PK

    更新日期：2016-05-18 00:00:00

  • Structural characterization of recombinant IAV polymerase reveals a stable complex between viral PA-PB1 heterodimer and host RanBP5.

    abstract：:The genome of influenza A virus (IAV) comprises eight RNA segments (vRNA) which are transcribed and replicated by the heterotrimeric IAV RNA-dependent RNA-polymerase (RdRp). RdRp consists of three subunits (PA, PB1 and PB2) and binds both the highly conserved 3'- and 5'-ends of the vRNA segment. The IAV RdRp is an imp...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep24727

    authors： Swale C,Monod A,Tengo L,Labaronne A,Garzoni F,Bourhis JM,Cusack S,Schoehn G,Berger I,Ruigrok RW,Crépin T

    更新日期：2016-04-20 00:00:00

  • Variance-corrected Michaelis-Menten equation predicts transient rates of single-enzyme reactions and response times in bacterial gene-regulation.

    abstract：:Many chemical reactions in biological cells occur at very low concentrations of constituent molecules. Thus, transcriptional gene-regulation is often controlled by poorly expressed transcription-factors, such as E.coli lac repressor with few tens of copies. Here we study the effects of inherent concentration fluctuati...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep17820

    authors： Pulkkinen O,Metzler R

    更新日期：2015-12-04 00:00:00

  • A soluble starch synthase I gene, IbSSI, alters the content, composition, granule size and structure of starch in transgenic sweet potato.

    abstract：:Soluble starch synthase I (SSI) is a key enzyme in the biosynthesis of plant amylopectin. In this study, the gene named IbSSI, was cloned from sweet potato, an important starch crop. A high expression level of IbSSI was detected in the leaves and storage roots of the sweet potato. Its overexpression significantly incr...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-02481-x

    authors： Wang Y,Li Y,Zhang H,Zhai H,Liu Q,He S

    更新日期：2017-05-24 00:00:00

  • 3D Printed Auxetic Mechanical Metamaterial with Chiral Cells and Re-entrant Cores.

    abstract：:By combining the two basic deformation mechanisms for auxetic open-cell metamaterials, re-entrant angle and chirality, new hybrid chiral mechanical metamaterials are designed and fabricated via a multi-material 3D printer. Results from mechanical experiments on the 3D printed prototypes and systematic Finite Element (...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-20795-2

    authors： Jiang Y,Li Y

    更新日期：2018-02-05 00:00:00

  • Doxorubicin-induced cardiomyopathy associated with inhibition of autophagic degradation process and defects in mitochondrial respiration.

    abstract：:Doxorubicin (Dox) is a highly effective anticancer drug but cause acute ventricular dysfunction, and also induce late-onset cardiomyopathy and heart failure. Despite extensive studies, the pathogenic sequelae leading to the progression of Dox-associated cardiomyopathy remains unknown. We assessed temporal changes in a...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-37862-3

    authors： Abdullah CS,Alam S,Aishwarya R,Miriyala S,Bhuiyan MAN,Panchatcharam M,Pattillo CB,Orr AW,Sadoshima J,Hill JA,Bhuiyan MS

    更新日期：2019-02-14 00:00:00

  • A humanized mouse model identifies key amino acids for low immunogenicity of H7N9 vaccines.

    abstract：:Influenza vaccines of H7N9 subtype are consistently less immunogenic in humans than vaccines developed for other subtypes. Although prior immunoinformatic analysis identified T-cell epitopes in H7 hemagglutinin (HA) which potentially enhance regulatory T cell response due to conservation with the human genome, the lin...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-01372-5

    authors： Wada Y,Nithichanon A,Nobusawa E,Moise L,Martin WD,Yamamoto N,Terahara K,Hagiwara H,Odagiri T,Tashiro M,Lertmemongkolchai G,Takeyama H,De Groot AS,Ato M,Takahashi Y

    更新日期：2017-04-28 00:00:00

  • Canine morbillivirus (canine distemper virus) with concomitant canine adenovirus, canine parvovirus-2, and Neospora caninum in puppies: a retrospective immunohistochemical study.

    abstract：:A retrospective immunohistochemical study was designed to investigate the frequency of concomitant traditional infectious disease pathogens in puppies that died suddenly and review the aspects of associated pathogenesis. Fifteen puppies were evaluated; the pathology reports and histopathologic slides of these animals ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-31540-0

    authors： Headley SA,Oliveira TES,Pereira AHT,Moreira JR,Michelazzo MMZ,Pires BG,Marutani VHB,Xavier AAC,Di Santis GW,Garcia JL,Alfieri AA

    更新日期：2018-09-07 00:00:00

  • Global-scale river flood vulnerability in the last 50 years.

    abstract：:The impacts of flooding are expected to rise due to population increases, economic growth and climate change. Hence, understanding the physical and spatiotemporal characteristics of risk drivers (hazard, exposure and vulnerability) is required to develop effective flood mitigation measures. Here, the long-term trend i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep36021

    authors： Tanoue M,Hirabayashi Y,Ikeuchi H

    更新日期：2016-10-26 00:00:00

  • Two distinct knockout approaches highlight a critical role for p53 in rat development.

    abstract：:Gene targeting in embryonic stem cells (ESCs) has become the principal technology for generating knockout models. Although numerous studies have predicted that the disruption of p53 leads to increased developmental anomalies and malignancies, most p53 knockout mice develop normally. Therefore, the role of p53 in anima...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep00945

    authors： Kawamata M,Ochiya T

    更新日期：2012-01-01 00:00:00

  • Unusual ferromagnetism enhancement in ferromagnetically optimal manganite La0.7-yCa0.3+yMn1-yRuyO3 (0≤y

    abstract：:The eg-orbital double-exchange mechanism as the core of physics of colossal magnetoresistance (CMR) manganites is well known, which usually covers up the role of super-exchange at the t2g-orbitals. The role of the double-exchange mechanism is maximized in La0.7Ca0.3MnO3, leading to the concurrent metal-insulator trans...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep09922

    authors： Liu MF,Du ZZ,Xie YL,Li X,Yan ZB,Liu JM

    更新日期：2015-04-24 00:00:00

  • Impaired Systolic and Diastolic Left Ventricular Function in Children with Chronic Kidney Disease - Results from the 4C Study.

    abstract：:Children with chronic kidney disease suffer from excessive cardiovascular mortality and early alterations of the cardiovascular system. Tissue doppler imaging is a validated echocardiographic tool to assess early systolic and diastolic cardiac dysfunction. We hypothesized that tissue Doppler velocities would reveal re...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-46653-3

    authors： Doyon A,Haas P,Erdem S,Ranchin B,Kassai B,Mencarelli F,Lugani F,Harambat J,Matteucci MC,Chinali M,Habbig S,Zaloszyc A,Testa S,Vidal E,Gimpel C,Azukaitis K,Kovacevic A,Querfeld U,Schaefer F

    更新日期：2019-08-07 00:00:00

  • Hypothermic Oxygenated Machine Perfusion of Liver Grafts from Brain-Dead Donors.

    abstract：:Hypothermic oxygenated machine perfusion (HOPE) was introduced in liver transplantation (LT) to mitigate ischemia-reperfusion injury. Available clinical data mainly concern LT with donors after circulatory-determined death, whereas data on brain-dead donors (DBD) are scarce. To assess the impact of end-ischemic HOPE i...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-45843-3

    authors： Patrono D,Surra A,Catalano G,Rizza G,Berchialla P,Martini S,Tandoi F,Lupo F,Mirabella S,Stratta C,Salizzoni M,Romagnoli R

    更新日期：2019-06-27 00:00:00

  • Immune complexes in chronic Chagas disease patients are formed by exovesicles from Trypanosoma cruzi carrying the conserved MASP N-terminal region.

    abstract：:The exovesicles (EVs) are involved in pathologic host-parasite immune associations and have been recently used as biomarkers for diagnosis of infectious diseases. The release of EVs by Trypanosoma cruzi, the causative agent of Chagas disease, has recently been described, with different protein cargoes including the MA...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep44451

    authors： Díaz Lozano IM,De Pablos LM,Longhi SA,Zago MP,Schijman AG,Osuna A

    更新日期：2017-03-15 00:00:00

  • Evaluation of Immunodiagnostic Tests for Leprosy in Brazil, China and Ethiopia.

    abstract：:Leprosy remains persistently endemic in several low- or middle income countries. Transmission is still ongoing as indicated by the unabated rate of leprosy new case detection, illustrating the insufficiency of current prevention methods. Therefore, low-complexity tools suitable for large scale screening efforts to spe...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-018-36323-1

    authors： van Hooij A,Tjon Kon Fat EM,Batista da Silva M,Carvalho Bouth R,Cunha Messias AC,Gobbo AR,Lema T,Bobosha K,Li J,Weng X,Salgado CG,Spencer JS,Corstjens PLAM,Geluk A

    更新日期：2018-12-18 00:00:00

  • Flavonol-induced changes in PIN2 polarity and auxin transport in the Arabidopsis thaliana rol1-2 mutant require phosphatase activity.

    abstract：:The phytohormone auxin is a major determinant and regulatory component important for plant development. Auxin transport between cells is mediated by a complex system of transporters such as AUX1/LAX, PIN, and ABCB proteins, and their localization and activity is thought to be influenced by phosphatases and kinases. Fl...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep41906

    authors： Kuhn BM,Nodzyński T,Errafi S,Bucher R,Gupta S,Aryal B,Dobrev P,Bigler L,Geisler M,Zažímalová E,Friml J,Ringli C

    更新日期：2017-02-06 00:00:00

  • Genome-wide identification and expression analysis of TCP transcription factors in Gossypium raimondii.

    abstract：:Plant-specific TEOSINTE-BRANCHED1/CYCLOIDEA/PCF (TCP) transcription factors play versatile functions in multiple aspects of plant growth and development. However, no systematical study has been performed in cotton. In this study, we performed for the first time the genome-wide identification and expression analysis of...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/srep06645

    authors： Ma J,Wang Q,Sun R,Xie F,Jones DC,Zhang B

    更新日期：2014-10-16 00:00:00

  • Nanoemulsion-loaded hydrogel coatings for inhibition of bacterial virulence and biofilm formation on solid surfaces.

    abstract：:The indiscriminate use of antibiotics has led to the emergence of drug-resistant bacteria which has become one of the biggest challenges of the twenty-first century for the researchers to combat and in turn search for novel targets which could lead to the development of effective and sustainable therapies. Inhibition ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-019-43016-w

    authors： Prateeksha,Barik SK,Singh BN

    更新日期：2019-04-24 00:00:00

  • Monkeys share the neurophysiological basis for encoding sound periodicities captured by the frequency-following response with humans.

    abstract：:The extraction and encoding of acoustical temporal regularities are fundamental for human cognitive auditory abilities such as speech or beat entrainment. Because the comparison of the neural sensitivity to temporal regularities between human and animals is fundamental to relate non-invasive measures of auditory proce...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-017-16774-8

    authors： Ayala YA,Lehmann A,Merchant H

    更新日期：2017-11-30 00:00:00

  • Comparisons of conventional and novel anthropometric obesity indices to predict metabolic syndrome among vegetarians in Malaysia.

    abstract：:Our study aimed to compare the ability of anthropometric obesity indices to predict MetS and to determine the sex-specific optimal cut-off values for MetS among Malaysian vegetarians. Body weight, height, waist circumference (WC), blood pressure (BP), fasting venous blood sample were collected from 273 vegetarians in ...

    journal_title：Scientific reports

    pub_type： 杂志文章

    doi：10.1038/s41598-020-78035-5

    authors： Ching YK,Chin YS,Appukutty M,Gan WY,Chan YM

    更新日期：2020-11-30 00:00:00