Abstract:
:A novel pullulanase gene, PulSS4, was identified from the gut microflora of Hermetia illucens by a function-based metagenome screening. The PulSS4 gene had an open reading frame of 4455 base pairs, and encoded a mature protein of 1484 amino acids, with a signal peptide sequence of 44 amino acids. The deduced amino acid sequence of PulSS4 gene showed 51% identity with that of the amylopullulanase of Amphibacillus xylanus, exhibiting no significant sequence homology to already known pullulanases. A conserved domain analysis revealed it to be a pullulanase type II with respective active sites at the N-terminal pullulanase and C-terminal amylase domain. PulSS4 was active in the temperature range of 10-50°C, with an optimum activity at 40°C. It was active in the pH range of 6.5-10.5, with optimum pH at 9.0, and retained more than 80% of its original activity in a broad pH range of 5-11 for 24h at 30°C. Also, PulSS4 was highly stable against many different chemical reagents, including 10% polar organic solvents and 1% non-ionic detergents. Overall, PulSS4 is expected to have the strong potential for application in biotechnological industries that require high activity at moderate temperature and alkaline conditions.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Lee YS,Seo SH,Yoon SH,Kim SY,Hahn BS,Sim JS,Koo BS,Lee CMdoi
10.1016/j.ijbiomac.2015.10.067subject
Has Abstractpub_date
2016-01-01 00:00:00pages
514-21eissn
0141-8130issn
1879-0003pii
S0141-8130(15)30070-2journal_volume
82pub_type
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