Identification of a novel alkaline amylopullulanase from a gut metagenome of Hermetia illucens.

Abstract:

:A novel pullulanase gene, PulSS4, was identified from the gut microflora of Hermetia illucens by a function-based metagenome screening. The PulSS4 gene had an open reading frame of 4455 base pairs, and encoded a mature protein of 1484 amino acids, with a signal peptide sequence of 44 amino acids. The deduced amino acid sequence of PulSS4 gene showed 51% identity with that of the amylopullulanase of Amphibacillus xylanus, exhibiting no significant sequence homology to already known pullulanases. A conserved domain analysis revealed it to be a pullulanase type II with respective active sites at the N-terminal pullulanase and C-terminal amylase domain. PulSS4 was active in the temperature range of 10-50°C, with an optimum activity at 40°C. It was active in the pH range of 6.5-10.5, with optimum pH at 9.0, and retained more than 80% of its original activity in a broad pH range of 5-11 for 24h at 30°C. Also, PulSS4 was highly stable against many different chemical reagents, including 10% polar organic solvents and 1% non-ionic detergents. Overall, PulSS4 is expected to have the strong potential for application in biotechnological industries that require high activity at moderate temperature and alkaline conditions.

journal_name

Int J Biol Macromol

authors

Lee YS,Seo SH,Yoon SH,Kim SY,Hahn BS,Sim JS,Koo BS,Lee CM

doi

10.1016/j.ijbiomac.2015.10.067

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

514-21

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(15)30070-2

journal_volume

82

pub_type

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