Abstract:
:Myoglobin is known to show formation of intermediate states under various environmental conditions, in spite of that, this is the first evidence of formation pre-molten globule (PMG) in myoglobin. Polyethylene glycol (PEG) of various molecular sizes shows assorted effects on different proteins. Out of too short and too long PEGs, only PEGs of optimal size interact with proteins leading to change in protein structure that form intermediate state. We are the first one to report the formation of PMG in a protein in the presence of a crowding agent. The PEG-induced intermediate state was characterized by various techniques like absorption, fluorescence, near- and far-UV circular dichroism spectroscopy, ANS binding, and dynamic light scattering measurements to be PMG. Isothermal titration calorimetry and docking studies were further carried out to delineate the mechanism of formation of PMG in myoglobin in physiological conditions. The intermediate formed due to interaction of PEG with myoglobin has physiological implications which are essential to unravel the mystery to solve the massively complicated problems involved in the proper folding of proteins in vivo. Further, outcomes from this study are expected to gain mechanistic insights on the native structure and functions of proteins under in vivo conditions.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Parray ZA,Ahamad S,Ahmad F,Hassan MI,Islam Adoi
10.1016/j.ijbiomac.2018.12.170subject
Has Abstractpub_date
2019-04-01 00:00:00pages
1288-1294eissn
0141-8130issn
1879-0003pii
S0141-8130(18)36095-1journal_volume
126pub_type
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