当前位置： SCI文献检索 > INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES期刊下所有文献 > Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus.

Molecular cloning and analysis of the full-length aciniform spidroin gene from Araneus ventricosus.

Abstract：

:Orb-web spiders produce more than seven different protein-based silks/glues by specialized abdominal glands for different uses. Prey-wrapping silk is secreted by aciniform glands for wrapping prey and forming the inner layer of egg case, and is almost twice as tough as other silks because of high strength and extensibility. So far, only two complete gene sequences have been obtained for aciniform spidroins (AcSp1). Here we describe the AcSp1 full-length gene sequence from the spider species Araneus ventricosus, using a long-distance PCR (LD-PCR) approach. The full-length AcSp1 gene is a single enormous exon of 10,338 bp in size, and the predicted protein sequence is 3445 amino acids long and consists of a conserved nonrepetitive N-terminal domain, a central predominantly repetitive region composed of fifteen repeat units, and a conserved nonrepetitive C-terminal domain.

journal_name

Int J Biol Macromol

journal_title

International journal of biological macromolecules

authors

Wen R,Wang K,Liu X,Li X,Mi J,Meng Q

doi

10.1016/j.ijbiomac.2017.12.090

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

1352-1360

eissn

0141-8130

issn

1879-0003

pii

S0141-8130(17)34826-2

journal_volume

117

pub_type

杂志文章

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