Abstract:
:Orb-web spiders produce more than seven different protein-based silks/glues by specialized abdominal glands for different uses. Prey-wrapping silk is secreted by aciniform glands for wrapping prey and forming the inner layer of egg case, and is almost twice as tough as other silks because of high strength and extensibility. So far, only two complete gene sequences have been obtained for aciniform spidroins (AcSp1). Here we describe the AcSp1 full-length gene sequence from the spider species Araneus ventricosus, using a long-distance PCR (LD-PCR) approach. The full-length AcSp1 gene is a single enormous exon of 10,338 bp in size, and the predicted protein sequence is 3445 amino acids long and consists of a conserved nonrepetitive N-terminal domain, a central predominantly repetitive region composed of fifteen repeat units, and a conserved nonrepetitive C-terminal domain.
journal_name
Int J Biol Macromoljournal_title
International journal of biological macromoleculesauthors
Wen R,Wang K,Liu X,Li X,Mi J,Meng Qdoi
10.1016/j.ijbiomac.2017.12.090subject
Has Abstractpub_date
2018-10-01 00:00:00pages
1352-1360eissn
0141-8130issn
1879-0003pii
S0141-8130(17)34826-2journal_volume
117pub_type
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journal_title:International journal of biological macromolecules
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