NMR studies on the solution structure of a deletion mutant of the transcarboxylase biotin carrier subunit.

Abstract:

:A deletion mutant of the transcarboxylase biotin carrier protein was completely labeled with 13C and 15N. A multitude of 2D and 3D NMR spectra were recorded and assigned. An NMR solution structure was derived from the data and compared in detail with the recently published structure of the wild-type. It is shown that deletion of 30% of the amino acids does not alter the structure of the rigid protein core.

journal_name

Int J Biol Macromol

authors

Jank MM,Sadowsky JD,Peikert C,Berger S

doi

10.1016/s0141-8130(02)00033-8

keywords:

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

233-42

issue

5

eissn

0141-8130

issn

1879-0003

pii

S0141813002000338

journal_volume

30

pub_type

杂志文章
  • It takes two to tango - The case of thebaine 6-O-demethylase.

    abstract::Thebaine 6-O-demethylase (T6ODM) is an Fe(II)/2-oxoglutarate-dependent dioxygenase catalysing two oxidative O-demethylation reactions in morphine biosynthesis. Its crystal structure revealed a large active site pocket which is at least two times larger than necessary to accommodate a substrate (thebaine or oripavine) ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.07.030

    authors: Kachhap S,Wojdyla Z,Komorek P,Kluza A,Kurpiewska K,Jachimska B,Borowski T

    更新日期:2020-11-15 00:00:00

  • Enhanced antibacterial activity of PEO-chitosan nanofibers with potential application in burn infection management.

    abstract::Chitosan (CS) is a well-known biological macromolecule having numerous applications due to its exceptional properties especially in the form of nanofibers. The antibacterial activity is compromised when co-polymers are added to electrospun CS but, the reproducibility can be enhanced which is key to commercialization. ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.06.022

    authors: Abid S,Hussain T,Nazir A,Zahir A,Ramakrishna S,Hameed M,Khenoussi N

    更新日期:2019-08-15 00:00:00

  • Preparation and characterization of contact active antibacterial surface based on chemically modified nanofibrillated cellulose by phenanthridinium silane salt.

    abstract::The main object of this research is chemical modification of the nanofibrillated cellulose (NFC) surface with a phenanthridinium silane salt to develop durable non-leaching antibacterial surface. Initially, (3-trimethoxysilylpropyl) phenanthridinium iodide (TMSPhI) as an antibacterial agent was synthesized using (3-ch...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.03.141

    authors: Hassanpour A,Asghari S,Mansour Lakouraj M,Mohseni M

    更新日期:2018-08-01 00:00:00

  • Biochemical, immunological and kinetic characterization and partial sequence analysis of a thiol proteinase inhibitor from Bubalus bubalis kidney: An attempt targeting kidney disorders.

    abstract::In the present study a thiol proteinase inhibitor was isolated from buffalo kidney making use of ammonium sulphate precipitation and gel filtration chromatography on Sephacryl S-100HR column. Purified inhibitor is homogeneous as it displayed a single band in gel electrophoresis both under reducing and non-reducing env...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.12.084

    authors: Shamsi A,Ahmed A,Bano B

    更新日期:2017-01-01 00:00:00

  • Activation effect of Ganoderma lucidum polysaccharides liposomes on murine peritoneal macrophages.

    abstract::The activation of murine peritoneal macrophages by Ganoderma lucidum polysaccharides liposomes (GLPL) was investigated in vitro. After treatment with GLPL, the changes of the nitric oxide (NO) secretion and iNOS (inducible nitric oxide synthase) activity were evaluated. The results showed that NO production and iNOS a...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.10.088

    authors: Liu Z,Xing J,Huang Y,Bo R,Zheng S,Luo L,Niu Y,Zhang Y,Hu Y,Liu J,Wu Y,Wang D

    更新日期:2016-01-01 00:00:00

  • Biomaterials cross-linked graphene oxide composite aerogel with a macro-nanoporous network structure for efficient Cr (VI) removal.

    abstract::Chitosan (CS) is an attractive bio-adsorbent in pollutant removal due to its environment-friendly properties and abundant adsorption sites. However, the weak mechanical properties and strong dissolubility in acidic conditions of CS hinder its wide application. Herein, a facile method was proposed to fabricate polydopa...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.11.174

    authors: Li L,Wei Z,Liu X,Yang Y,Deng C,Yu Z,Guo Z,Shi J,Zhu C,Guo W,Sun Z

    更新日期:2020-08-01 00:00:00

  • Polyhydroxybutyrate by Streptomyces sp.: Production and characterization.

    abstract::A total number of 20 actinomycetes isolates were isolated from soil sediments obtained from Semmancheri coastal areas of Bay of Bengal, India and they were qualitatively screened for the production of polyhydroxybutyrate (PHB) on a medium containing Sudan black stain. Nine of the 20 isolates produced PHB and the quant...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.07.028

    authors: Krishnan S,Chinnadurai GS,Perumal P

    更新日期:2017-11-01 00:00:00

  • Novel insights into the treatment of SARS-CoV-2 infection: An overview of current clinical trials.

    abstract::The emergence of the global pandemic caused by the novel SARS-CoV-2 virus has motivated scientists to find a definitive treatment or a vaccine against it in the shortest possible time. Current efforts towards this goal remain fruitless without a full understanding of the behavior of the virus and its adaptor proteins....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2020.09.204

    authors: Oroojalian F,Haghbin A,Baradaran B,Hemmat N,Shahbazi MA,Baghi HB,Mokhtarzadeh A,Hamblin MR

    更新日期:2020-12-15 00:00:00

  • Down-regulated miR-187 promotes oxidative stress-induced retinal cell apoptosis through P2X7 receptor.

    abstract::Several microRNAs (miRNAs) expressed in the retina were confirmed to involve in retinal cell apoptosis, which was closely linked with the development of retinal diseases. Our previous studies have confirmed a vital role of miR-187 in retinal cells apoptosis. The aim of this study was to further elucidate the precise r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.08.166

    authors: Zhang QL,Wang W,Alatantuya,Dongmei,Lu ZJ,Li LL,Zhang TZ

    更新日期:2018-12-01 00:00:00

  • Recent advances on polysaccharides, lipids and protein based edible films and coatings: A review.

    abstract::Food is a vital product for the survival of human beings and with passage of time quality concerns of consumers are rising. Edible films and coatings are thin layers applied on food products to protect them and improve their quality. Films/coatings are prepared from naturally occurring renewable sources (polysaccharid...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章,评审

    doi:10.1016/j.ijbiomac.2017.11.097

    authors: Hassan B,Chatha SAS,Hussain AI,Zia KM,Akhtar N

    更新日期:2018-04-01 00:00:00

  • Effects of ionic polysaccharides and egg white protein complex formulations on dough rheological properties, structure formation and in vitro starch digestibility of wet sweet potato vermicelli.

    abstract::Effects of ionic polysaccharides [chitosan (C), sodium alginate (A) and xanthan (X)] and egg white protein (EP) complex formulations on dough rheology, structure formation and in vitro starch digestibility of wet sweet potato vermicelli (SPV) were investigated. Linear viscoelastic region (LVR) with low strain level of...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.02.020

    authors: Feng YY,Mu TH,Zhang M,Ma MM

    更新日期:2020-04-15 00:00:00

  • Identification, structure analysis, and transcript profiling of purple acid phosphatases under Pi deficiency in tomato (Solanum lycopersicum L.) and its wild relatives.

    abstract::Purple acid phosphatases (PAPs), a family of metallo-phosphoesterase enzymes, are involved in phosphorus nutrition in plants. In this study, we report that the tomato genome encodes 25 PAP members. Physio-biochemical analyses revealed relatively lower total root-associated acid phosphatase activity in the seedlings of...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.10.080

    authors: Srivastava R,Akash,Parida AP,Chauhan PK,Kumar R

    更新日期:2020-12-15 00:00:00

  • Vital insights into prokaryotic genome compaction by nucleoid-associated protein (NAP) and illustration of DNA flexure angles at single-molecule resolution.

    abstract::Integration Host Factor (IHF) is a heterodimeric site-specific nucleoid-associated protein (NAP), well known for its DNA bending ability. Although the IHF induced bending states of DNA have been captured by both X-ray Crystallography and Atomic Force Microscopy (AFM), the range of flexibility and degree of heterogenei...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.12.194

    authors: Purkait D,Bandyopadhyay D,Mishra PP

    更新日期:2021-01-05 00:00:00

  • The structure property and adsorption capacity of new enzyme-treated potato and sweet potato starches.

    abstract::To explore a valuable new enzyme method that effectively changes the molecular structure and absorption property of potato and sweet potato starch granules, the common combination of α-amylase (AA) and glucoamylase (GA), namely AA → GA (common method as contrast), the combination of glycosyltransferase (GT) and GA, na...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.09.164

    authors: Guo L,Li J,Li H,Zhu Y,Cui B

    更新日期:2020-02-01 00:00:00

  • Green synthesis of biopolymer-silver nanoparticle nanocomposite: an optical sensor for ammonia detection.

    abstract::Biopolymer used for the production of nanoparticles (NPs) has attracted increasing attention. In the presence article we use aqueous solution of polysaccharide Cyamopsis tetragonaloba commonly known as guar gum (GG), from plants. GG acts as reductive preparation of silver nanoparticles which are found to be <10 nm in ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2012.06.033

    authors: Pandey S,Goswami GK,Nanda KK

    更新日期:2012-11-01 00:00:00

  • Cellulosic fabric treated with hyperbranched polyethyleneimine derivatives for improving antibacterial, dyeing, pH and thermo-responsive performance.

    abstract::Having cotton fabrics with multifunctional properties is of the most research focused on using either different processes or new and different materials. Improving thermo - responsive and antibacterial properties of cotton fabrics decorated with silver nanoparticles and nanogel has been investigated. During this resea...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.12.198

    authors: Mohamed AL,Hassabo AG

    更新日期:2021-02-15 00:00:00

  • Revealing of sugar utilization systems in Halomonas sp. YLGW01 and application for poly(3-hydroxybutyrate) production with low-cost medium and easy recovery.

    abstract::Poly(3-hydroxybutyrate) (PHB) is a common polyhydroxyalkanoate (PHA) with potential as an alternative for petroleum-based plastics. Previously, we reported a new strain, Halomonas sp. YLGW01, which hyperproduces PHB with 94% yield using fructose. In this study, we examined the PHB production machinery of Halomonas sp....

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.11.163

    authors: Park YL,Song HS,Choi TR,Lee SM,Park SL,Lee HS,Kim HJ,Bhatia SK,Gurav R,Park K,Yang YH

    更新日期:2021-01-15 00:00:00

  • Biotic and abiotic stress induces the expression of Hsp70/90 organizing protein gene in silkworm, Bombyx mori.

    abstract::The heat shock 70/90 organizing protein is one of the stress-induced proteins and has extensively been studied in mammals. It mediates the interaction of Hsp70 and Hsp 90 as cochaperone and also implicated in infection-related processes. However, the biological role of heat shock 70/90 organizing protein in silkworm r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.12.067

    authors: Kausar S,Abbas MN,Yang L,Cui H

    更新日期:2020-01-15 00:00:00

  • A mechanistic investigation on methotrexate-loaded chitosan-based hydrogel nanoparticles intended for CNS drug delivery: Trojan horse effect or not?

    abstract::Chitosan-based hydrogel nanoparticles provide a higher brain concentration of methotrexate (MTX) following IV administration in comparison with the drug's simple solution. The present study investigates the mechanism of this phenomenon, focusing on the possible role of P-gp. A previously developed MTX containing chito...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.12.093

    authors: Pourtalebi Jahromi L,Moghaddam Panah F,Azadi A,Ashrafi H

    更新日期:2019-03-15 00:00:00

  • Influence of lignin level on release of hemicellulose-derived sugars in liquid hot water.

    abstract::Lignin layers surrounding hemicelluloses and cellulose in the plant cell walls protect them from deconstruction. This recalcitrance to sugar release is a major limitation for cost-effective industrial conversion of lignocellulosic biomass to biofuels. Many literatures had reported the contribution of lignin removal to...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.10.045

    authors: Yu Q,Zhuang X,Yuan Z,Kong X,Qi W,Wang W,Wang Q,Tan X

    更新日期:2016-01-01 00:00:00

  • Investigation of the nanofibrillar morphology in silk fibers by small angle X-ray scattering and atomic force microscopy.

    abstract::Small angle X-ray scattering (SAXS) and atomic force microscopy (AFM) measurements have been shown to be consistent with the presence of nanofibrils in the cocoon silk of Bombyx mori and the dragline silk of Nephila clavipes. The transverse dimensions and correlation lengths range from > 59 to 220 nm and in the axial ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/s0141-8130(99)00024-0

    authors: Miller LD,Putthanarat S,Eby RK,Adams WW

    更新日期:1999-03-01 00:00:00

  • The antitumor activity of a red alga polysaccharide complexes carrying 5-fluorouracil.

    abstract::Porphyran is a sulfated galactan isolated from red algae Porphyra haitanensis, and have been reported to have many kinds of biological activities such as antitumor activity. In order to provide a water-soluble macromolecule prodrug of 5-Fu showing slow release of 5-Fu and reducing side-effect, we carried out fixation ...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2014.06.017

    authors: Wang X,Zhang Z

    更新日期:2014-08-01 00:00:00

  • Deciphering the interactions of fish gelatine and hyaluronic acid in aqueous solutions.

    abstract::The interactions of fish gelatine (FG) with hyaluronic acid (HA) are studied in an aqueous environment at 25°C by turbidimetric titration, confocal scanning laser microscopy, dynamic light scattering, zeta potentiometry, spectrophotometry with methylene blue, and construction of state diagrams. FG forms soluble comple...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2017.04.083

    authors: Razzak MA,Kim M,Kim HJ,Park YC,Chung D

    更新日期:2017-09-01 00:00:00

  • Genomic characterization provides genetic evidence for bacterial cellulose synthesis by Acetobacter pasteurianus RSV-4 strain.

    abstract::There is ongoing quest to look for alternate sustainable and renewable biopolymers which can address the existing environmental issues. Bacterial cellulose could be one such option. Several organisms have been reported to produce bacterial cellulose. Among this, acetic acid bacteria (AAB) are reported to be one of the...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.04.078

    authors: Thakur K,Kumar V,Kumar V,Yadav SK

    更新日期:2020-08-01 00:00:00

  • Kinetics of irreversible inhibition of yeast alcohol dehydrogenase during modification by 4,4'-dithiodipyridine.

    abstract::The course of inactivation of yeast alcohol dehydrogenase (YADH) using 4,4'-dithiodipyridine (DSDP) has been studied in this paper. The results show that the reaction mechanism between DSDP and YADH is a competitive, complexing inhibition. The microscopic constants for the inactivation of the free enzyme and the enzym...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/s0141-8130(97)00030-5

    authors: Zheng SY,Xu D,Wang HR,Li J,Zhou HM

    更新日期:1997-07-01 00:00:00

  • Production and characterization of exopolysaccharides from Chlorella zofingiensis and Chlorella vulgaris with anti-colorectal cancer activity.

    abstract::The production, physicochemical characteristics, antioxidant and antitumor activities of partial purified exopolysaccharides from Chlorella zofingiensis, Chlorella vulgaris were investigated. The final exopolysaccharides productions were 208.4, 364.3 mg/L and the average molecular weights were 2.66×104, 1.88×104 Da, r...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2019.05.117

    authors: Zhang J,Liu L,Chen F

    更新日期:2019-08-01 00:00:00

  • Self-assembling crystals of an extract of Flos Sophorae Immaturus for improving the antioxidant, mechanical and barrier properties of a cassia gum film.

    abstract::A novel antioxidative film was prepared by drying a film-forming solution containing the Flos Sophorae Immaturus extract (FSIE) (0-3.5%) and cassia gum (CG). Scanning electron microscopy (SEM) showed that FSIE was successfully compounded with CG. Although the addition of FSIE slightly increased the water vapor permeab...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2020.11.082

    authors: Meng F,Zhang C,Li J,Wang L

    更新日期:2021-01-15 00:00:00

  • Neuroprotective effects of Coptis chinensis Franch polysaccharide on amyloid-beta (Aβ)-induced toxicity in a transgenic Caenorhabditis elegans model of Alzheimer's disease (AD).

    abstract::This study aims to investigate the neuroprotective effects of Coptis chinensis Franch polysaccharide (CCP) on Aβ1-42 transgenic CL4176 Caenorhabditis elegans, as well as its mechanism of action. The results in life span experiment showed that CCP could significantly increase the lifespan of C. elegans and the effect i...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2018.03.035

    authors: Li Y,Guan S,Liu C,Chen X,Zhu Y,Xie Y,Wang J,Ji X,Li L,Li Z,Zhang Y,Zeng X,Li M

    更新日期:2018-07-01 00:00:00

  • Synthesized negatively charged macromolecules (NCMs) for the surface modification of anticoagulant membrane biomaterials.

    abstract::A series of negatively charged macromolecules (NCMs) including poly (sulfonated styrene-co-methyl methacrylate) (P(SS-co-MMA)), poly (acrylic acid-co-methyl methacrylate) (P(AA-co-MMA)) and poly (sulfonated styrene-co-acrylic acid-co-methyl methacrylate) (P(SS-co-AA-co-MMA)) are synthesized by reversible addition frag...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2013.01.014

    authors: Ran F,Nie S,Yin Z,Li J,Su B,Sun S,Zhao C

    更新日期:2013-04-01 00:00:00

  • Durable antibacterial and UV protections of in situ synthesized zinc oxide nanoparticles onto cotton fabrics.

    abstract::Herein we represent a new discovery based on amine material called hexamethyltriethylene tetramine (HMTETA). We have observed that when an aqueous solution of Zn(NO3)·6H2O was added to aqueous solution of HMTETA followed by shaking for a time, the colorless solution was converted to milky color under the alkaline medi...

    journal_title:International journal of biological macromolecules

    pub_type: 杂志文章

    doi:10.1016/j.ijbiomac.2015.11.003

    authors: Shaheen TI,El-Naggar ME,Abdelgawad AM,Hebeish A

    更新日期:2016-02-01 00:00:00