Cetuximab-resistant oral squamous cell carcinoma cells become sensitive in anchorage-independent culture conditions through the activation of the EGFR/AKT pathway.


:We have previously shown that growth of the oral squamous cell carcinoma cell line SAS, is resistant to cetuximab in monolayer culture conditions, even though epidermal growth factor receptor (EGFR) was phosphorylated, but the growth of SAS aggregates was sensitive to cetuximab. In the present study, we demonstrate differences in the EGFR signaling pathways utilized by SAS cells in monolayer and suspension cultures at the molecular level. Cetuximab treatment of SAS cells in monolayer cultures inhibits the phosphorylation of EGFR and ERK, and reduces the cell migratory potency, but not cell proliferation. AG1478 treatment reduces the phosphorylation of EGFR, ERK and AKT, and affects cell growth in monolayer cultures. The phosphorylation levels of EGFR and AKT are significantly higher in SAS cell aggregates compared to monolayer cultures. Treatment with cetuximab and AG1478 reduces the growth of SAS aggregates and eliminates the phosphorylation of EGFR and AKT. Furthermore, proliferation of SAS aggregates is also inhibited by LY294002 and MK2206, which are inhibitors of PI3K and AKT, respectively. In addition, treatment with the lipid raft disruptor filipin III reduced the phosphorylation levels of EGFR and Akt in SAS aggregates, but not in SAS monolayer culture. These results suggest the possibility that ligands in the serum stimulate the phosphorylation of EGFR localized in lipid rafts leading to PI3K-AKT activation, which results in the growth of SAS aggregates, therefore resulting in the sensitivity of SAS aggregates to cetuximab.


Int J Oncol


Ohnishi Y,Yasui H,Kakudo K,Nozaki M




Has Abstract


2015-12-01 00:00:00












  • DNA damage detected with gammaH2AX in endometrioid adenocarcinoma cell lines.

    abstract::Phosphorylation of histone H2AX (gammaH2AX) is a sensitive marker of DNA damage, particularly induction of DNA double-strand breaks. Using multiparameter cytometry we explored the effects of doxorubicin (DOX), cisplatin (CDDP) and 5-fluorouracil (5-FU) on four types of endometrioid adenocarcinoma cell lines (HEC-1A, H...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ikeda M,Kurose A,Takatori E,Sugiyama T,Traganos F,Darzynkiewicz Z,Sawai T

    更新日期:2010-05-01 00:00:00

  • PRL-3 improves colorectal cancer cell proliferation and invasion through IL-8 mediated glycolysis metabolism.

    abstract::Phosphatase of regenerating liver-3 (PRL-3) has been found to be overexpressed in liver metastases of colorectal cancer and rarely expressed in primary tumors, which plays an important role in the metastasis of colorectal cancer cells. Metabolism reprogramming has been found to be a hallmark of cancer cells, and aerob...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Xu H,Zeng Y,Liu L,Gao Q,Jin S,Lan Q,Lai W,Luo X,Wu H,Huang Y,Chu Z

    更新日期:2017-10-01 00:00:00

  • Colon cancer stem cell markers CD44 and CD133 in patients with colorectal cancer and synchronous hepatic metastases.

    abstract::CD44 and CD133 mRNA expression as cancer stem cell markers in colorectal cancer were correlated with synchronous hepatic metastases and the clinicopathological factors, including patient survival. The CD44 and CD133 mRNA levels in 36 primary colorectal adenocarcinomas with synchronous hepatic metastasis were analyzed ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Jing F,Kim HJ,Kim CH,Kim YJ,Lee JH,Kim HR

    更新日期:2015-04-01 00:00:00

  • The mechanism of epithelial-mesenchymal transition induced by TGF-β1 in neuroblastoma cells.

    abstract::Neuroblastoma is the second most common extracranial malignant solid tumor that occurs in childhood, and metastasis is one of the major causes of death in neuroblastoma patients. The epithelial-mesenchymal transition (EMT) is an important mechanism for both the initiation of tumor invasion and subsequent metastasis. T...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Shao JB,Gao ZM,Huang WY,Lu ZB

    更新日期:2017-05-01 00:00:00

  • Enhanced eradication of local and distant tumors by genetically produced interleukin-12 and radiation.

    abstract::Ionizing radiation (IR) is frequently unsuccessful in the treatment of cancer because of local failure or distant metastases. The efficacy of systemically administered cytokines for cancer therapy is often limited by toxicity. We report that intratumoral injection of an adenoviral vector with interleukin-12 (IL-12) en...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Seetharam S,Staba MJ,Schumm LP,Schreiber K,Schreiber H,Kufe DW,Weichselbaum RR

    更新日期:1999-10-01 00:00:00

  • Salinomycin and its derivatives as potent RET transcriptional inhibitors for the treatment of medullary thyroid carcinoma.

    abstract::Rearranged during transfection kinase (RET) is a validated molecular target in medullary thyroid cancer (MTC), as activating mutations in RET are often associated with the development of MTC. The present study reports the first preclinical characterization of salinomycin and selected analogs as potent RET transcriptio...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Alqahtani T,Kumarasamy VM,Huczyński A,Sun D

    更新日期:2020-01-01 00:00:00

  • High COX-2 expression contributes to a poor prognosis through the inhibition of chemotherapy-induced senescence in nasopharyngeal carcinoma.

    abstract::Resistance to radiotherapy and chemotherapy currently represents one of the major reasons for therapeutic failure in nasopharyngeal carcinoma (NPC). However, the mechanisms underlying resistance to chemotherapy in NPC remain unclear. In this study, cell counting assay, cell cycle assay and senescence associated β-gala...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Shi C,Guan Y,Zeng L,Liu G,Zhu Y,Xu H,Lu Y,Liu J,Guo J,Feng X,Zhao X,Jiang W,Li G,Li G,Dai Y,Jin F,Li W,Zhou W

    更新日期:2018-09-01 00:00:00

  • The androgen receptor in hormone-refractory prostate cancer: relevance of different mechanisms of androgen receptor signaling (Review).

    abstract::The last decade has brought increased awareness to prostate cancer as a significant health problem. Prostate cancer is very heterogeneous in its etiology and progression, but androgen signaling appears to be a common key element in its development and progression. Blocking of androgen signaling results in a decrease i...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: Cronauer MV,Schulz WA,Burchardt T,Anastasiadis AG,de la Taille A,Ackermann R,Burchardt M

    更新日期:2003-10-01 00:00:00

  • Effects of estrogen and tamoxifen on the MAP kinase cascade in experimental rat breast cancer.

    abstract::The mitogen-activated protein kinase (MAPK) cascade, which includes MAPK, MAP kinase kinase (MAPKK) and Raf-l, is involved in the signal transduction of growth factor receptors. We found that the MAPK and Raf-l proteins are increased in human breast cancer. Activated MAPKK was also observed. We then investigated wheth...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Koibuchi Y,Iino Y,Uchida T,Nagasawa M,Morishita Y

    更新日期:1997-09-01 00:00:00

  • Cell-cycle regulator cyclin D1 mRNA and protein overexpression occurs in primary malignant gliomas.

    abstract::Gliomas are malignant brain tumors thought to arise through multi-step tumorigenesis, involving both the activation of oncogenes and the loss of tumor suppressor genes. The cyclin D1 gene encodes a proto-oncogenic cell cycle regulator implicated in the pathogenesis of several types of cancer, including gliomas. Northe...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Maxwell M,Galanopoulos T,Antoniades H

    更新日期:1996-09-01 00:00:00

  • Regulation of COX-2 expression and epithelial-to-mesenchymal transition by hypoxia-inducible factor-1α is associated with poor prognosis in hepatocellular carcinoma patients post TACE surgery.

    abstract::Currently, it is not entirely clear whether hypoxia-inducible factor-1α (HIF-1α) is involved in the regulation of COX-2 expression and epithelial-to-mesenchymal transition (EMT), and whether these events affect the prognosis of hepatocellular carcinoma (HCC) patients treated with transcatheter arterial chemoembolizati...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Huang M,Wang L,Chen J,Bai M,Zhou C,Liu S,Lin Q

    更新日期:2016-05-01 00:00:00

  • In vitro antitumour activity and cellular pharmacological properties of the platinum-iminoether complex trans-[PtCl2[E-HN=C(OMe)Me]2].

    abstract::The platinum complex trans-[PtCl2¿E-HN=C(OMe)Me¿2] was compared to cisplatin for cytotoxicity towards tumour cells, and for cellular pharmacological properties in A2780 and cisplatin-resistant A2780/Cp8 ovarian cancer cells. Trans-[PtCl2¿E-HN=C(OMe)Me¿2] was comparably cytotoxic to cisplatin (mean IC50 after 72 h expo...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Coluccia M,Nassi A,Boccarelli A,Giordano D,Cardellicchio N,Intini FP,Natile G,Barletta A,Paradiso A

    更新日期:1999-11-01 00:00:00

  • Functional impact of colorectal cancer-associated mutations in the transcription factor E2F4.

    abstract::The transcription factor E2F4 plays a critical role in cell cycle progression of normal and cancerous intestinal epithelial cells. Contrary to other E2Fs, the coding region of the E2F4 gene contains a longer spacer segment of a CAG trinucleotide repeat sequence encoding 13 consecutive serine residues, which is highly ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Paquin MC,Leblanc C,Lemieux E,Bian B,Rivard N

    更新日期:2013-12-01 00:00:00

  • [Corrigendum] USP22 promotes the G1/S phase transition by upregulating FoxM1 expression via β‑catenin nuclear localization and is associated with poor prognosis in stage II pancreatic ductal adenocarcinoma.

    abstract::Subsequently to the publication of the above paper, the authors have realized that Figs. 3 and 4 contained errors. Two sets of bands in Figs. 3D and 4B were erroneously placed during the process of figure preparation. In Fig. 3D, the β‑actin bands for the PANC‑1 and CFPAC‑1 cells were presented incorrectly, whereas in...

    journal_title:International journal of oncology

    pub_type: 杂志文章,已发布勘误


    authors: Ning Z,Wang A,Liang J,Xie Y,Liu J,Feng L,Yan Q,Wang Z

    更新日期:2019-11-01 00:00:00

  • A dynamic interplay between alternative polyadenylation and microRNA regulation: implications for cancer (Review).

    abstract::Alternative polyadenylation and microRNA regulation are both mechanisms of post-transcriptional regulation of gene expression. Alternative polyadenylation often results in mRNA isoforms with the same coding sequence but different lengths of 3' UTRs, while microRNAs regulate gene expression by binding to specific mRNA ...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: An J,Zhu X,Wang H,Jin X

    更新日期:2013-10-01 00:00:00

  • The p53-positive phenotype of breast cancers.

    abstract::One hundred and eighty-one breast cancer specimens were analyzed for nuclear p53 staining by immunochemical methods. There were 123 fine-needle cytological specimens and 58 frozen tissue sections of surgical biopsies. The microscopic evaluation of the staining fitted with a 4 group classification. Ninety-one samples (...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Lesec G,Richardcoulet E,Collet J,Demaublanc M,Merle S,Salmon R,Remvikos Y

    更新日期:1997-04-01 00:00:00

  • Effects of endostatin production on oncogenicity and metastatic activity of HPV16-transformed mouse cells: role of interleukin 1alpha.

    abstract::Two mouse HPV16-transformed cell lines, viz. MK16 cells, which induce metastasizing tumors, and TC-1 cells, which induce non-metastasizing tumors were transduced with the gene for mouse endostatin. Two clones constitutively expressing endostatin were isolated from each of them. They were denoted ME3 and ME9, and TE2 a...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Lakatosova-Andelova M,Duskova M,Lucansky V,Paral P,Vonka V

    更新日期:2009-07-01 00:00:00

  • New aromatase inhibitors in the treatment of advanced breast cancer.

    abstract::New aromatase inhibitors are an exciting treatment option for postmenopausal women with hormone sensitive breast cancer. They have been shown to reduce tumors in a significant number of patients, and exhibit definite antitumor activity at a relatively low daily dose, and are highly potent, highly selective, and well-t...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: Crucitta E,Lorusso V,Attolico M,Sambiasi D,Mazzei A,De Lena M

    更新日期:2000-11-01 00:00:00

  • Tumor oxygenation and radiosensitization by pentoxifylline and a perflubron emulsion carbogen breathing.

    abstract::Oxygen tension measurements were made in three tumors: (i) the murine FSaII fibrosarcoma, (ii) the rat 9L gliosarcoma and (iii) the rat 13672 mammary adenocarcinoma using a pO2 histograph. Tumor oxygenation measurements were made while the animals breathed air or breathed carbogen (95% oxygen/5% carbon dioxide). Pento...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Teicher B,Sotomayor E,Robinson M,Dupuis N,Schwartz G,Frei E

    更新日期:1993-01-01 00:00:00

  • Update on uveal melanoma: Translational research from biology to clinical practice (Review).

    abstract::Uveal melanoma is the most common type of intraocular cancer with a low mean annual incidence of 5‑10 cases per million. Tumours are located in the choroid (90%), ciliary body (6%) or iris (4%) and of 85% are primary tumours. As in cutaneous melanoma, tumours arise in melanocytes; however, the characteristics of uveal...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ortega MA,Fraile-Martínez O,García-Honduvilla N,Coca S,Álvarez-Mon M,Buján J,Teus MA

    更新日期:2020-12-01 00:00:00

  • Efficient induction of specific cytotoxic T lymphocytes to tumor rejection peptide using functional matured 2 day-cultured dendritic cells derived from human monocytes.

    abstract::Dendritic cells (DCs) are powerful antigen-presenting cells (APCs), that have so far been applied for cancer specific immunotherapy. Recent results suggest that matured DCs derived from human monocytes have a significant impact on the outcome of vaccination. The conventional generation of mature DCs from human monocyt...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Tanaka F,Yamaguchi H,Haraguchi N,Mashino K,Ohta M,Inoue H,Mori M

    更新日期:2006-11-01 00:00:00

  • Factors influencing the expression of endogenous reverse transcriptases and viral-like 30 elements in mouse NIH3T3 cells.

    abstract::Retroviral reverse transcriptase (RT) plays a definite role in retroviral life cycle and is essential for the process of retrotransposition. We investigated the RNA expression of endogenous reverse transcriptases (enRTs) in the NIH3T3 mouse genome using, as a probe, a mixture of RT-PCR generated reverse transcriptase ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Tzavaras T,Eftaxia S,Tavoulari S,Hatzi P,Angelidis C

    更新日期:2003-10-01 00:00:00

  • Further aspects on cellular and cordless telephones and brain tumours.

    abstract::We included in a case-control study on brain tumours and mobile and cordless telephones 1,617 patients aged 20-80 years of both sexes diagnosed during January 1, 1997 to June 30, 2000. They were alive at the study time and had histopathology verified brain tumour. One matched control to each case was selected from the...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Hardell L,Mild KH,Carlberg M

    更新日期:2003-02-01 00:00:00

  • Loss of heterozygosity on chromosome 17p13 (p53) and 13q14 (Rb1) in squamous cell carcinoma of head and neck.

    abstract::Loss of chromosome sequences at 13q14 (Rbl) and 17p13 (p53) associated with squamous cell carcinoma of head and neck (SCCHN) was evaluated in 12 recurrent tumors and 51 primary tumors from 63 patients. The incidence of LOH at 17p13 was 19 of 50 (38%) tumors, and at 13q14 was 21 of 57 (37%). LOH affecting Rbl and/or p5...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Li Y,Pavelic Z,Wang L,Wang X,Gleich L,Biddinger P,Gartside P,Wilson K,Gluckman J,Stambrook P

    更新日期:1997-06-01 00:00:00

  • Antitumor effect of YM155, a novel small-molecule survivin suppressant, via mitochondrial apoptosis in human MFH/UPS.

    abstract::Survivin is a member of the inhibitor of apoptosis family, which is known to inhibit mitochondrial apoptosis. Survivin is highly expressed in cancers and plays an important role in cancer cell survival, and increased survivin expression is an unfavorable prognostic marker in cancer patients. YM155, a novel small-molec...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Minoda M,Kawamoto T,Ueha T,Kamata E,Morishita M,Harada R,Toda M,Onishi Y,Hara H,Kurosaka M,Akisue T

    更新日期:2015-09-01 00:00:00

  • Deleterious MnSOD signals lead to abnormal breast cell proliferation by radiation and estrogen exposure.

    abstract::Manganese superoxide dismutase (MnSOD) seems to have a pivotal role in maintaining the normal phenotype by suppressing cell growth through blocking the entrance of quiescent cells into the cell cycle. MnSOD protein expression has been shown to be dysregulated in malignant cells. A well-established experimental breast ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Echiburú-Chau C,Roy D,Calaf GM

    更新日期:2011-06-01 00:00:00

  • Endosialin: a novel malignant cell therapeutic target for neuroblastoma.

    abstract::Endosialin emerged recently as a potential therapeutic target for sarcoma. Since some sarcoma subtypes, such as Ewing's sarcoma, show characteristics of neuroendocrine differentiation, we wondered whether cancers with neuro-endocrine properties and/or neuroectodermal origin, such as neuroblastoma, small cell lung canc...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Rouleau C,Smale R,Sancho J,Fu YS,Kurtzberg L,Weber W,Kruger A,Jones C,Roth S,Bormann C,Dunham S,Krumbholz R,Curiel M,Wallar G,Mascarello J,Campos-Rivera J,Horten B,Schmid S,Miller G,Teicher BA

    更新日期:2011-10-01 00:00:00

  • Overcoming the classical multidrug resistance phenotype by adenoviral delivery of anti-MDR1 short hairpin RNAs and ribozymes.

    abstract::Simultaneous resistance of cancer cells to multiple cytotoxic drugs, multidrug resistance (MDR), is the major limitation to the successful chemotherapeutic treatment of disseminated neoplasms. The 'classical' MDR phenotype is conferred by MDR1/P-glycoprotein (MDR1/P-gp) that is expressed in almost 50% of human cancers...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kaszubiak A,Holm PS,Lage H

    更新日期:2007-08-01 00:00:00

  • Peripheral blood tumor cell load reflects the clinical activity of the disease in patients with carcinoma of the breast.

    abstract::Peripheral blood was examined for the presence of tumor cells at multiple time points over a one-year period in 45 patients with a history of surgical resection of breast carcinoma. The number of circulating epithelial cells in 8 of 8 patients with clinically active disease concurred or preceded changes in the disease...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Terstappen LW,Rao C,Gross S,Weiss AJ

    更新日期:2000-09-01 00:00:00

  • Functional characteristics and gene expression profiles of primary acute myeloid leukaemia cells identify patient subgroups that differ in susceptibility to histone deacetylase inhibitors.

    abstract::Modulation of gene expression through histone deacetylase (HDAC) inhibition is considered a possible therapeutic strategy in acute myeloid leukaemia (AML). In vitro effects and basal gene expression of structurally different HDAC inhibitors were examined. Primary human AML cells were derived from 59 consecutive patien...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Stapnes C,Ryningen A,Hatfield K,Øyan AM,Eide GE,Corbascio M,Kalland KH,Gjertsen BT,Bruserud Ø

    更新日期:2007-12-01 00:00:00