Abstract:
:Aberrant glycosylation is a universal feature of cancer cells, and certain glycan structures are well-known markers for tumor progression. Availability and composition of sugars in the microenvironment may affect cell glycosylation. Recent studies of human breast tumor cell lines indicate their ability to take up and utilize fructose. Here we tested the hypothesis that adding fructose to culture as a carbon source induces phenotypic changes in cultured human breast tumor cells that are associated with metastatic disease. MDA-MB-468 cells were adapted to culture media in which fructose was substituted for glucose. Changes in cell surface glycan structures, expression of genes related to glycan assembly, cytoskeleton F-actin, migration, adhesion and invasion were determined. Cells cultured in fructose expressed distinct cell-surface glycans. The addition of fructose affected sialylation and fucosylation patterns. Fructose feeding also increased binding of leukoagglutinating Phaseolus vulgaris isolectin, suggesting a possible rise in expression of branching beta-1, 6 GlcNAc structures. Rhodamine-phalloidin staining revealed an altered F-actin cytoskeletal system. Fructose accelerated cellular migration and increased invasion. These data suggest that changing the carbon source of the less aggressive MDA-MB-468 cell line induced characteristics associated with more aggressive phenotypes. These data could be of fundamental importance due to the markedly increased consumption of sweeteners containing free fructose in recent years, as they suggest that the presence of fructose in nutritional microenvironment of tumor cells may negatively affect the outcome for some breast cancer patients.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Monzavi-Karbassi B,Hine RJ,Stanley JS,Ramani VP,Carcel-Trullols J,Whitehead TL,Kelly T,Siegel ER,Artaud C,Shaaf S,Saha R,Jousheghany F,Henry-Tillman R,Kieber-Emmons Tdoi
10.3892/ijo_00000710subject
Has Abstractpub_date
2010-09-01 00:00:00pages
615-22issue
3eissn
1019-6439issn
1791-2423journal_volume
37pub_type
杂志文章abstract::Low concentrations of monoclonal antibodies (mAb) specific for the CD3 component of the T cell receptor complex (TcR) may be both immunosuppressive or potent activators of T cell function in vitro depending upon the experimental situation. The anti-CD3 mAb OKT3 is imnmunosuppressive at high doses (5 mg/day for 10 to 1...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.3.675
更新日期:1994-09-01 00:00:00
abstract::Bcl-xL is an apoptosis inhibitor that is upregulated in bladder cancer (BCa) and provides an attractive target for molecular therapies. Treatment with specific antisense oligodeoxynucleotides (AS‑ODNs) or small interfering RNAs (siRNAs) were able to sensitize BCa cells to conventional chemotherapeutics. Ten new Bcl‑xL...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3096
更新日期:2015-09-01 00:00:00
abstract::NGFI-B, Nurr1 and NOR-1 have structural features of ligand-activated transcriptional regulators and constitute the NGFI-B subfamily within the nuclear receptor superfamily. Since specific ligands for these molecules have not yet been identified, they are often called orphan nuclear receptors. Genes of the NGFI-B subfa...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.12.6.1237
更新日期:1998-06-01 00:00:00
abstract::This study defines the role of WWOX in the regulation of epithelial to mesenchymal transition. A group of 164 endometrial adenocarcinoma patients was studied as well as an ECC1 well-differentiated steroid-responsive endometrial cell line, which was transducted with WWOX cDNA by a retroviral system. The relationship be...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2964
更新日期:2015-01-01 00:00:00
abstract::Accumulating evidence has demonstrated the crucial roles of long non‑coding RNAs (lncRNAs) in various human cancers, including non‑small cell lung cancer (NSCLC). However, to the best of our knowledge, the role of the lncRNA cancer susceptibility candidate 7 (CASC7) in NSCLC has not been clearly determined. The aim of...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5076
更新日期:2020-08-01 00:00:00
abstract::Spindle cell tumors are clinically heterogeneous but morphologically similar neoplasms that can occur anywhere, mostly in adult patients. They are treated primarily with surgery to which is often added adjuvant or neoadjuvant radiation. Sub-classification of spindle cell sarcomas requires integration of histology, cli...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2605
更新日期:2014-11-01 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is a major cause of cancer deaths. Concurrent administration of radiation and chemotherapy is the treatment of choice for advanced NPC. Previously, we showed that apogossypolone (ApoG2) induced apoptosis by blocking the binding of Bcl-2 to Bax, arresting the cell cycle in the S phase, in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2497
更新日期:2014-09-01 00:00:00
abstract::Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3643
更新日期:2016-10-01 00:00:00
abstract::The interactions between cells and the extracellular matrix (EM) have important effects on tumor invasion and metastasis. Osteosarcoma (OS) is a highly metastatic tumor, secondary lesions occurring early in the natural history of the disease. Despite the clinical relevance of disseminated disease in this neoplasia, th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.5.963
更新日期:1993-11-01 00:00:00
abstract::Neuroblastoma (NB) is the most common extra-cranial solid tumor in children and despite aggressive therapy survival rates remain low. One of the contributing factors for low survival rates is aggressive tumor angiogenesis, which is known to increase due to radiation, one of the standard therapies for neuroblastoma. Th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3646
更新日期:2016-10-01 00:00:00
abstract::In a recent study, we evaluated the efficacy and toxicity of 5-fluorouracil (5-FU), cisplatin (CDDP), and 5-FU plus CDDP (FP treatment) in gastric cancer cell lines and examined the relationship between the response to FP treatment and apoptosis. Our study indicated that there may be prognostic value in measuring p53 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::We have examined six patients with multiple low grade, low stage superficial multifocal bladder tumors with surrounding tissues for loss of heterozygosity (LOH) and microsatellite instability at chromosome 3, totaling 76 samples. The majority (4/5) of the patients had LOH at or close to the fragile histidine triad (FH...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.1.203
更新日期:2001-01-01 00:00:00
abstract::As epidemiological data suggest ultraviolet (UV) radiation to be the major environmental factor for the development of melanoma, we screened this malignancy for UV-specific C right curved arrow T and CC right curved arrow TT DNA mutations as described in squamous and basal cell carcinomas of the skin. Mitochondrial (m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Cancer represents a severe challenge to healthcare systems and individuals worldwide. The development of multiple drug resistance is a major issue regarding cancer therapy, which can result in the progression of disease. Cholesterol is a major constituent of cell membranes and participates in the regulation of several...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5124
更新日期:2020-09-22 00:00:00
abstract::Tumor cell invasion and metastasis requires precise coordination of adherence to the extracellular matrix (ECM) and controlled degradation of its components. lnvasive cells secrete proteolytic enzymes known as matrix metalloproteinases which degrade specific basement membrane molecules. Expression of these enzymes is ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.6.1127
更新日期:1998-12-01 00:00:00
abstract::Recently, a novel candidate tumor suppressor gene, DPC 4/SMAD 4, has been implicated in the development of pancreatic cancers. Its location at human chromosome 18q21 prompted us to investigate this gene in a large series of primary tumors located outside the gastrointestinal tract which have been associated with loss ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1997-02-01 00:00:00
abstract::Multidrug-resistance (MDR) is largely caused by the efflux of therapeutics from the tumor cell by means of P-glycoprotein (P-gp), resulting in reduced efficacy of the chemotherapy. In order to overcome MDR, substances, such as verapamil and cyclosporin A (CsA), were employed. As these P-gp modulating agents did not se...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.23.6.1505
更新日期:2003-12-01 00:00:00
abstract::We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.3.433
更新日期:2000-09-01 00:00:00
abstract::Increased expression levels of the long non-coding RNA metastasis-associated lung adenocarcinoma transcript 1 (Malat1) have been associated with enhanced proliferation and metastasis of several cancer cell types. Hypoxia, a hallmark characteristic of solid tumors, has been linked to an increase in the activity of the ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3630
更新日期:2016-10-01 00:00:00
abstract::Recently, ultrasound-guided percutaneous ethanol injection therapy (PEIT) has been widely performed in the treatment of hepatocellular carcinoma. Histopathologic examinations after the therapy have revealed that PEIT can destroy the tumor completely in most cases. Findings in imaging modalities and serum tumor marker ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.4.669
更新日期:1993-04-01 00:00:00
abstract::With a view to identifying markers reflecting not the evidence of, but the potential for, neoplastic progression of the breast we have compared the normal invasive mammary epithelial cell growth seen at puberty with invasive and non-invasive carcinogenesis using the mouse as a model. We have analyzed cell proliferatio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Carnosic acid is a phenolic diterpene with anti-inflammation, anticancer, anti-bacterial, anti-diabetic, as well as neuroprotective properties, which is generated by many species from Lamiaceae family. Fisetin (3,3',4',7-tetrahydroxyflavone), a naturally flavonoid is abundantly produced in different vegetables and fru...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3970
更新日期:2017-06-01 00:00:00
abstract::The effects of a thymic peptide preparation (TP) on the immunocytotoxicity and cytokine secretion of peripheral blood lymphocytes and monocytes from patients with colorectal tumors, breast tumors and melanoma were studied in vitro. On average, breast tumor and melanoma patients showed significantly lower natural kille...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.3.481
更新日期:1997-03-01 00:00:00
abstract::Angiogenic factors are major causes of tumor progression in hematological malignancies, particularly multiple myeloma, as well as solid tumors. The introduction of thalidomide as an anti-angiogenic agent in myeloma treatment has demonstrated the importance of angiogenic factors in the progression of myeloma. However, ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::Erucylphosphocholine (ErPC) is a promising antineoplastic drug for the treatment of malignant brain tumors. It exerts strong anticancer activity and induces apoptosis even in chemoresistant glioma cells. In the present study, A172 and U373MG glioma cells were treated with ErPC to explore the contribution of MAP kinase...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::In the multistep progressive pathogenesis of human breast cancer, comedo ductal carcinoma in situ (DCIS) represents a preinvasive precursor lesion for therapy resistant invasive cancer. Human tissue derived cell culture models exhibiting molecular similarities to clinical DCIS facilitate an important preclinical mecha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00
abstract::Cutaneous infantile hemangioma progresses through proliferation and involution phases. Since treatment with interferon, a negative regulator of angiogenesis, accelerates the involution phase, we hypothesized that cutaneous infantile hemangioma is associated with an imbalance between endogenous positive and negative re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.3.401
更新日期:1999-03-01 00:00:00
abstract::High lactate production in cells during growth under oxygen-rich conditions (aerobic glycolysis) is a hallmark of tumor cells, indicating the role of mitochondrial function in tumorigenesis. In fact, enhanced mitochondrial biogenesis and impaired quality control are frequently observed in cancer cells. Mitochondrial D...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2018.4468
更新日期:2018-09-01 00:00:00
abstract::Human pancreatic ductal adenocarcinomas overexpress the epidermal growth factor (EGF) receptor. Betacellulin is a mitogenic polypeptide that binds and activates this receptor. To determine whether betacellulin has a role in human pancreatic cancer, we studied its expression in cultured human pancreatic cancer cell lin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.4.825
更新日期:1995-10-01 00:00:00
abstract::The transcription factor twist family bHLH transcription factor 1 (TWIST), which is a member of the basic helix-loop-helix class of proteins, is known to induce epithelial-mesenchymal transition (EMT) and promote cancer metastasis. TWIST has previously been reported to be associated with multidrug resistance (MDR), si...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4495
更新日期:2018-10-01 00:00:00