Abstract:
:Spindle cell tumors are clinically heterogeneous but morphologically similar neoplasms that can occur anywhere, mostly in adult patients. They are treated primarily with surgery to which is often added adjuvant or neoadjuvant radiation. Sub-classification of spindle cell sarcomas requires integration of histology, clinicopathological parameters, immunohistochemistry, cytogenetics (including fluorescence in situ hybridization) and/or molecular genetics. Some of the tumor subtypes are characterized by the presence of distinct chromosomal translocations and fusion genes. When no signs of differentiation are seen, the diagnosis by exclusion becomes undifferentiated spindle cell sarcoma. Cytogenetic, RNA sequencing and RT-PCR analyses were performed on a case of spindle cell sarcoma. The karyotype of the primary tumor was 46,X,del(X)(p?11p?22), der(12)(12pter→12q?22::12q?15→q?22::16p11→16pter),-16,+r(12). MDM2 was found amplified in both the primary tumor and a meta-stasis. RNA-Seq of the primary tumor identified four fusion genes, PTGES3-PTPRB, HMGA2-DYRK2, TMBIM4-MSRB3 and USP15-CNTN1, in which all the partner genes map to the q arm of chromosome 12. In material from the metastasis, RT-PCR detected the PTGES3-PTPRB, HMGA2-DYRK2 and TMBIM4-MSRB3 whereas no USP15-CNTN1 fusion transcript was found. Because MDM2 amplification and the fusion transcripts PTGES3-PTPRB, HMGA2-DYRK2 and TMBIM4-MSRB3 were found both in the primary tumor and in the metastasis, they are components of the same clone and may be involved both in initiation and progression of the tumor. Which of them is pathogenetically primary remains unknown.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Panagopoulos I,Bjerkehagen B,Gorunova L,Berner JM,Boye K,Heim Sdoi
10.3892/ijo.2014.2605subject
Has Abstractpub_date
2014-11-01 00:00:00pages
1829-36issue
5eissn
1019-6439issn
1791-2423journal_volume
45pub_type
杂志文章abstract::Linear accelerator-based radiotherapy has little effect on tumors such as malignant melanoma, various types of sarcoma, and most locally-advanced neoplasms that have grown to several centimeters or more. These tumors contain many hypoxic cancer cells or large amounts of anti-oxidative enzymes, and are therefore resist...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:10.3892/ijo.2011.1069
更新日期:2011-09-01 00:00:00
abstract::It has been reported that MUC-1 molecules devoid of the tandem repeat region (MUC-1/Y) are detected preferentially in carcinoma cells and are associated with their progression. However, its clinical significance is still unknown. We constructed a mouse colon adenocarcinoma cell line (MC-38) transduced with either MUC-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.5.1085
更新日期:2001-05-01 00:00:00
abstract::Insulin-like growth factor-binding protein-2 (IGFBP-2) is considered to be a human tumor antigen, and the tumor-specific immunity of IGFBP-2 has been reported in several types of cancer. The purpose of this study was to evaluate whether autoantibodies to IGFBP-2 can be used as diagnostic markers in lung cancer. The re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1699
更新日期:2013-01-01 00:00:00
abstract::Whether elderly patients with metastatic esophageal, gastroesophageal, and gastric cancer do as well with chemotherapy as their younger counterparts was investigated in this pooled analysis. In total, 367 patients from 8 consecutive, first-line trials were included: i) etoposide + cisplatin; ii) 5-fluorourucil + leuco...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000535
更新日期:2010-03-01 00:00:00
abstract::We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.3.433
更新日期:2000-09-01 00:00:00
abstract::The active metabolite of vitamin D, 1alpha,25-dihydroxyvitamin D3, can induce differentiation in breast cancer cells; however, it is hypercalcemic in vivo. Therefore, development of non-calcemic analogs of vitamin D has received considerable attention. Recently, we synthesized an analog of vitamin D [1alpha(OH)D5] tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.1.65
更新日期:2000-01-01 00:00:00
abstract::We have analyzed 18 families with high incidence of breast cancer or breast and ovarian cancer for the presence of BRCA1 mutations. We identified 4 mutations in the BRCA1 gene in 4 unrelated probands who belong to families with at least 1 case of breast and 1 case of ovarian cancer. Two of the mutations reported in th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.20.5.963
更新日期:2002-05-01 00:00:00
abstract::Seventy-one patients with metastatic colorectal carcinoma(CRC) were treated with varying doses of the mouse monoclonal antibody (MAb) 17-1A. One patient achieved a partial remission (PR) (1%) with a survival duration of 114+ months. Further 10 patients showed a minor response (MR) or stable disease > 3 months (SD) (14...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.5.1049
更新日期:1995-11-01 00:00:00
abstract::Clinicopathological assessment of the functional relationship between the HER2 oncogene and tumor-associated fatty acid synthase (FASN) is largely precluded because immunohistochemical and/or mRNA studies should be performed in biopsies from breast cancer patients. We here sought to determine whether serum FASN (sFASN...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000455
更新日期:2009-12-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF), a secreted endothelial-specific growth factor, and microvessel density was examined in 204 primary non-small cell lung carcinomas. The expression of VEGF was evaluated by immunohistochemical staining using an anti-VEGF antibody. Positive staining was obtained in 125 out of 20...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.1059
更新日期:1995-05-01 00:00:00
abstract::Cholangiocarcinoma (CC) is an aggressive liver tumor with limited therapeutic options. Natrium‑iodide symporter (NIS) mediates the uptake of iodine by the thyroid, representing a key component in metabolic radiotherapy using iodine‑131 (131I) for the treatment of thyroid cancer. NIS expression is increased in CC, prov...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.4957
更新日期:2020-03-01 00:00:00
abstract::In this study we investigated the relationship between the development of either HSILs (high-grade squamous intraepithelial lesions) or invasive cancers of the uterine cervix and the p53 codon 72 polymorphisms consisting of arginine (Arg)- or proline (Pro)-encoded allele in Japanese populations. Furthermore, we determ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Newcastle disease virus (NDV) is an avian paramyxovirus with oncolytic properties which shows promising effects in the treatment of cancer. Anti-cancer effects are due to the virus ability: i) to replicate in and kill tumor cells, leading finally to their selective elimination; and ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1222
更新日期:2012-01-01 00:00:00
abstract::The CXC chemokine receptor 7 (CXCR7) has been reported to be involved in cell growth, metastasis and apoptosis in certain cancers. However, the function and molecular mechanisms of CXCR7 in human colorectal cancer (CRC) are still undefined. In the present study, sixty-eight cases of CRC tissues and corresponding adjac...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2547
更新日期:2014-10-01 00:00:00
abstract::Angiogenesis is increased in various human cancers, including head and neck squamous cell carcinoma (HNSCC), and correlates with tumor progression and metastasis. Vascular endothelial growth factor (VEGF) has been shown to be a key regulator of angiogenesis. We determined whether VEGF antisense oligonucleotide treatme...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::An endocrine disrupting chemical (EDC) is a global health concern. In this study, we examined the effects of genistein (GEN) on bisphenol A (BPA) or 17β-estradiol (E2)-induced cell growth and gene alterations of BG-1 ovarian cancer cells expressing estrogen receptors (ERs). In an in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1719
更新日期:2013-02-01 00:00:00
abstract::Malignant peripheral nerve sheath tumors (MPNSTs) are aggressive soft tissue tumors arising sporadically although more frequently in patients with Neurofibromatosis type 1. Prognosis remains dismal as chemo- and radiotherapy have not been shown to be successful. The heparin-binding growth factor, Midkine (MK), is impl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-11-01 00:00:00
abstract::A disintegrin and metalloproteinase-17 (ADAM17) can cut and release a wide variety of epidermal growth factor receptor (EGFR) ligands to promote survival, invasion and proliferation of cancer cell, and therefore, is considered to be a potential therapeutic target for cancer. The main goal of the present study was to o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3536
更新日期:2016-08-01 00:00:00
abstract::Glioma is the most prevalent and fatal primary tumor of the central nervous system in adults, while the development of effective therapeutic strategies in clinical practice remain a challenge. Nucleotide-binding domain leucine-rich family pyrin-containing 3 (NLRP3) has been reported to be associated with tumorigenesis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4480
更新日期:2018-09-01 00:00:00
abstract::Degradation of the extracellular matrix is a prerequisite for the invasive phenotype in glioma cells. Several proteases released by invading tumor cells seem to participate in the focal degradation of extracellular matrix proteins. Using enzymatic assays, Western blotting, and Northern blotting techniques, we investig...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.3.519
更新日期:2001-09-01 00:00:00
abstract::Tumor cell (TC) metastasis is initiated by selective adhesion of TCs to target structures such as basement membrane and endotheliaI cells (ECs), followed by transvascular migration of TCs. Variants of murine B16 melanoma having different metastatic potentials (in the order BL6 greater than or equal to F10>F1>WA4) have...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.2.319
更新日期:1995-02-01 00:00:00
abstract::Our study reviews the central role of androgens for the development of androgen-insensitive growth in prostatic carcinoma using an individual dynamic model which emulates aspects of proliferation control. Our aim is the identification of individually proliferating cells from prostate carcinoma. Short-term cultured cel...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.6.1339
更新日期:1995-12-01 00:00:00
abstract::Breast cancer is the leading cause of cancer death among women. Paclitaxel, a mitotic inhibitor, is highly effective in the treatment of breast cancer. However, development of resistance to paclitaxel limits its clinical use. Identifying new compounds and new strategies that are effective against breast cancer, in par...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3871
更新日期:2017-03-01 00:00:00
abstract::The p53 homologue p73 is overexpressed in many tumors, including lung cancer. We have evaluated the differential expression and subcellular localization of the functionally distinct apoptotic (TA) and anti-apoptotic (DeltaN) isoforms of p73 in non-small cell lung cancer (NSCLC), their possible association with p53 exp...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
abstract::IL-10 has been shown to play a crucial role in immunosuppression in cancer patients. We explored the regulation of IL-10 production by TNF-alpha, IL-1 beta, IL-6, IL-8, and IFN-gamma in human colon carcinoma COLO205 cells. Northern analysis revealed a marked expression of IL-10 mRNA after stimulation by IL-6, and a ma...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::In order to optimize the management of patients with renal cell carcinoma (RCC) it is important to define the genetic risk for metastatic disease. In this study we performed comparative genomic hybridization (CGH) on metastatic tumors aiming at the identification of genetic alterations associated with metastatic disea...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.5.903
更新日期:2000-11-01 00:00:00
abstract::To evaluate the significance of microsatellite instability (MI) and loss of heterozygosity (LOH) in the development of different histological subgroups of liposarcomas, we examined 28 tissue-samples from 21 patients and the corresponding non-neoplastic reference tissues. We investigated nine microsatellite loci and de...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.4.721
更新日期:1999-04-01 00:00:00
abstract::In the study of 69 matched pairs of lung tumors and normal lung parenchyma, the median activities of cathepsin B (CB) and cystein proteinase inhibitors (CPI's) in tumors were found to be increased 4.4-fold and twofold, respectively. The levels of CB and CPI activities did not correlate with TNM stages nor with cell di...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.1.77
更新日期:1994-07-01 00:00:00
abstract::Activation of the Wingless (Wnt)/ß-catenin signaling pathway contributes to prostate tumorigenesis and metastasis. Depending of the stage of prostate cancer development, current drug therapies are of limited efficiency, so that prevention with natural compounds appears as an attractive strategy especially due to the s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.905
更新日期:2011-03-01 00:00:00
abstract::An overview of the current mechanisms identified as associated with the expression of multidrug resistance is provided. The clinical relevance of multidrug resistance associated with the overexpression of the three major drug resistance-associated proteins namely, P-glycoprotein, MRP and/or LRP, is discussed. Alternat...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00