Abstract:
:The CXC chemokine receptor 7 (CXCR7) has been reported to be involved in cell growth, metastasis and apoptosis in certain cancers. However, the function and molecular mechanisms of CXCR7 in human colorectal cancer (CRC) are still undefined. In the present study, sixty-eight cases of CRC tissues and corresponding adjacent non-cancer tissues (ANCT) were collected, and the expression of CXCR7 was assessed using immunohistochemistry (IHC) in biopsy samples. Furthermore, CXCR7 gene was silenced by small hairpin RNA-mediated lentiviral vector (Lv-shCXCR7), by transfection into human CRC cells (SW480 and HT-29). The levels of p-ERK, β-arrestin, proliferating cell nuclear antigen (PCNA), matrix metallopeptidase-2 (MMP-2) and caspase-3 (CAS-3) were detected by western blotting. Cell proliferative activities and invasive capability were respectively measured by MTT and Transwell assays. Cell apoptosis was analyzed by flow cytometry. The results demonstrated that CXCR7 expression was significantly upregulated in CRC tissues compared with the ANCT (54.4 vs. 36.8%, P=0.041), and correlated with Dukes staging and depth of invasion (P=0.007; P=0.002). Silencing of CXCR7 gene suppressed cell proliferation and invasion, and induced cell apoptosis in CRC cells with decreased expression of p-ERK, β-arrestin, PCNA and MMP-2 but increased expression of CAS-3. The tumor volumes in the SW480 subcutaneous tumor models treated with Lv-shCXCR7 were significantly smaller than those of the negative control (NC) and PBS groups (P<0.01). In conclusion, our findings indicate that upregulation of CXCR7 expression is associated with tumor invasion, and silencing of the CXCR7 gene represses the development of CRC cells through ERK and β-arrestin pathways, suggesting that CXCR7 may serve as a potential therapeutic target for the treatment of CRC.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Li XX,Zheng HT,Huang LY,Shi DB,Peng JJ,Liang L,Cai SJdoi
10.3892/ijo.2014.2547subject
Has Abstractpub_date
2014-10-01 00:00:00pages
1649-57issue
4eissn
1019-6439issn
1791-2423journal_volume
45pub_type
杂志文章abstract::Medulloblastoma is the most common malignant brain tumor in childhood. The most prevalent chromosomal abnormalities are isochromosome 17q and loss of 17p, the location of the tumor suppressor gene p53. Mutations in the p53 gene in medulloblastoma are relatively infrequent but have recently been correlated to poor prog...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2010.884
更新日期:2011-03-01 00:00:00
abstract::Placenta-specific protein 1 (PLAC1) expression is co-opted in numerous human cancers. As a consequence of PLAC1 expression, tumor cells exhibit enhanced proliferation and invasiveness. This characteristic is associated with increased aggressiveness and worse patient outcomes. Recently, the presence of the tumor suppre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3931
更新日期:2017-05-01 00:00:00
abstract::Raf-1 protein kinase plays an important role in cell growth, proliferation and cell survival. We have previously described the use of liposome-entrapped antisense raf oligonucleotide (LErafAON) to inhibit Raf-1 expression resulting in tumor growth inhibition and radiosensitization. The present study was undertaken to ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-05-01 00:00:00
abstract::Estrogens act through interactions with estrogen receptors (ERs) to play diverse roles in various pathophysiological conditions. A number of synthetic selective estrogen receptor modulators (SERMs), such as tamoxifen and raloxifene, have been developed and used to treat ER-related diseases, including breast cancer and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3002
更新日期:2015-07-01 00:00:00
abstract::Multiple myeloma (MM) patients initially respond to conventional therapy, however, many develop resistance and have recurrences. We have reported in other tumors that the transcription factor Yin Yang 1 (YY1) is a resistant factor and, thus, we hypothesized that YY1 may be over-expressed in MM. Significantly, higher e...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2511
更新日期:2014-09-01 00:00:00
abstract::MicroRNAs can govern up to hundred different mRNAs and are important regulators of gene expression programs in development and disease. We analyzed the expression of microRNA-21, one of the most common oncomirs, in non-small cell lung cancer (NSCLC). Using northern blots the microRNA-21 expression levels of NSCLC-deri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2272
更新日期:2014-04-01 00:00:00
abstract::Epithelial-mesenchymal transition (EMT) is associated with tumor malignancy. The hedgehog-EMT pathway is preferentially activated in diffuse-type gastric cancer (GC) compared with intestinal-type GC; however, histological typing is currently the only method for distinguishing these two major types of GC. We compared t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2387
更新日期:2014-06-01 00:00:00
abstract::Phosphorylation of histone H2AX (gammaH2AX) is a sensitive marker of DNA damage, particularly induction of DNA double-strand breaks. Using multiparameter cytometry we explored the effects of doxorubicin (DOX), cisplatin (CDDP) and 5-fluorouracil (5-FU) on four types of endometrioid adenocarcinoma cell lines (HEC-1A, H...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000589
更新日期:2010-05-01 00:00:00
abstract::Patients who have inoperable lung cancer usually undergo chemotherapy and have problems such as a resistance against chemotherapeutic agents during the treatment. Glutathione S-transferase (GST) is one of the detoxication-related enzymes. We studied the relationship between immunohisto-chemical staining of GST-pi type...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.1.127
更新日期:1997-07-01 00:00:00
abstract::Currently, it is not entirely clear whether hypoxia-inducible factor-1α (HIF-1α) is involved in the regulation of COX-2 expression and epithelial-to-mesenchymal transition (EMT), and whether these events affect the prognosis of hepatocellular carcinoma (HCC) patients treated with transcatheter arterial chemoembolizati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3421
更新日期:2016-05-01 00:00:00
abstract::Whether elderly patients with metastatic esophageal, gastroesophageal, and gastric cancer do as well with chemotherapy as their younger counterparts was investigated in this pooled analysis. In total, 367 patients from 8 consecutive, first-line trials were included: i) etoposide + cisplatin; ii) 5-fluorourucil + leuco...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000535
更新日期:2010-03-01 00:00:00
abstract::Recombinant adenovirus-associated vector serotype 2 (rAAV2) is one of the most promising gene transfer vectors due to its advantage of causing non-pathogenic infection, low immunogenicity, and long-term gene expression in human clinical trials. Human interleukin 15 (hIL15) has been implicated in modulation of antitumo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-02-01 00:00:00
abstract::Human telomerase is a ribonucleoprotein DNA polymerase which maintains the telomeric region of human chromosomes and has been detected in all types of human cancer tested. We used the telomeric repeat amplification protocol (TRAP) assay to examine 71 non-small cell lung carcinomas (NSCLC) and their adjacent normal tis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.5.961
更新日期:1999-11-01 00:00:00
abstract::MicroRNAs (miRNAs) are a large family of small, non-coding RNAs that play a pivotal role in tumorigenesis. miR‑34a, which is a member of the miR-34 family, is a downstream target of p53. Increasing evidence shows that miR-34a dysregulation may contribute to tumor development and progression in numerous cancers, includ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4015
更新日期:2017-07-01 00:00:00
abstract::Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable mar...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4223
更新日期:2018-02-01 00:00:00
abstract::Pro‑inflammatory (M1) macrophages have key roles in atherogenesis. As β‑glucan has been demonstrated to exert pro‑inflammatory effects, the present study examined whether β‑glucan exerts atherogenic effects via converting macrophages into M1 phenotype. The results from reverse transcription‑quantitative polymerase cha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4630
更新日期:2019-01-01 00:00:00
abstract::Enhanced Notch signaling contributes to uncontrolled cell growth and cell death resistance in cancer. Here, we demonstrate that in colorectal carcinoma cells the Notch1-dependent activation of cell cycle and proliferation is mediated by repression of the cyclin-dependent kinase inhibitor (CDKI) p27. The half-life of p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2140
更新日期:2013-12-01 00:00:00
abstract::Seventy-two archival specimens of advanced gastric adenocarcinoma were analyzed by fluorescence in situ hybridization and immunohistochemistry for HER2 gene status. Gene amplification and concomitant protein overexpression were detected in 15.2% of cases. Intratumor topographical variability of HER2 amplification was ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Chronic administration of 17 beta-estradiol to male Syrian hamsters for at least six months induces estrogen-dependent kidney tumors, which express high levels of c-fos mRNA compared to surrounding renal or control tissues. We have investigated the cellular localization of c-Fos oncoprotein in these tissues and studie...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.3.527
更新日期:1995-09-01 00:00:00
abstract::Whilst the role of microRNA‑143 (miR‑143) in myelodysplastic syndrome (MDS) remains unclear, abnormally expressed microRNA‑143 has been detected in many types of cancer tissues. In this study, we describe a cohort study for the verification of miR‑143 expression, as well as the investigation of the molecular mechanism...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4534
更新日期:2018-11-01 00:00:00
abstract::In the present study, a series of seven synthetic croma-kalim analogues were prepared and evaluated for cytotoxic effect on human cervical carcinoma HeLa cells using WST-8 assay. A preliminary screening of these cromakalim analogues showed that 1-[(3S,4R)-4-(2-ethoxy-4-methyl-1H-pyrrol-1-yl)-3-hydroxy- 2,2-dimethylchr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1153
更新日期:2011-12-01 00:00:00
abstract::Neuroblastoma is a childhood malignancy originating from cells of the sympathetic nervous system, exhibiting a marked diversity in outcome, with spontaneous regression at one end of the spectrum and severe disease and death at the other end. Features associated with frequent recurrence, a poor prognosis, and high tumo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.5.943
更新日期:2000-05-01 00:00:00
abstract::We previously developed a new microscopic observation system that enables time-lapse quantitative analysis of apoptosis and necrosis. With this system we quantitatively analyzed adriamycin (ADR)-induced cell death using manganese superoxide dismutase (MnSOD)- and wild-type p53-gene transfectants on SaOS(2), a p53-defi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::On the basis of immunological results, it is not in doubt that the immune system is able to recognize and eliminate transformed cells. A plethora of studies have investigated the immune system of patients with cancer and how it is prone to immunosuppression, due in part to the decrease in lymphocyte proliferation and ...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2018.4283
更新日期:2018-04-01 00:00:00
abstract::Signal transducers and activators of transcription 3 (STAT3) signaling is persistently activated in many types of cancer cells, and represents a valid target for anticancer drug design. However, few reports have described the constitutive activation of STAT3 in human sarcoma cells. In this study, we demonstrate that t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Postoperative adjuvant chemotherapy reportedly improves advanced colorectal cancer patients' survival, however, it is necessary to assess what regimens are useful. Doxifluridine (5'-DFUR) is an intermediate of capecitabine approved in Europe and USA to treat metastatic colorectal cancer. 5'-DFUR is metabolized to 5-fl...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2003-07-01 00:00:00
abstract::The prognostic significance of Ki 67/MIB1 immunohistochemical assays (ICAs) was investigated in optimal technical conditions in 139 breast carcinomas. Automated ICAs (immunoperoxidase/Ventana device) was performed on frozen sections. Immunoprecipitates were quantified by computerized analysis (SAMBA) of digitized micr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.9.2.337
更新日期:1996-08-01 00:00:00
abstract::Using two malignant rat glioma cell lines, we tested to what extent peripheral immunization could affect tumor growth in the brain of syngeneic rats. Peripheral subcutaneous (s.c.) immunization was performed with autologous Newcastle-disease-virus (NDV)-infected or non-infected live tumor cells. Thus immunized rats or...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.4.759
更新日期:1995-04-01 00:00:00
abstract::Polo‑like kinase 4 (PLK4) has been identified as an oncogene, which is overexpressed in various types of human cancer; however, its role in colorectal cancer (CRC) development remains unknown. The present study demonstrated that PLK4 protein expression was upregulated in CRC tissues compared with in normal tissues thr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4659
更新日期:2019-02-01 00:00:00
abstract::The representative halophyte Calystegia soldanella (L) Roem. et Schult is a perennial vine herb that grows in coastal dunes throughout South Korea as well as in other regions around the world. This plant has long been used as an edible and medicinal herb to cure rheumatic arthritis, sore throat, dropsy, and scurvy. So...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3836
更新日期:2017-02-01 00:00:00