High PLK4 expression promotes tumor progression and induces epithelial‑mesenchymal transition by regulating the Wnt/β‑catenin signaling pathway in colorectal cancer.


:Polo‑like kinase 4 (PLK4) has been identified as an oncogene, which is overexpressed in various types of human cancer; however, its role in colorectal cancer (CRC) development remains unknown. The present study demonstrated that PLK4 protein expression was upregulated in CRC tissues compared with in normal tissues through western blotting. In addition, immunohistochemical analysis of 39 CRC specimens further demonstrated that PLK4 protein expression was upregulated in 64.1% (25/39) of samples. Increased PLK4 expression was closely associated with enhanced tumor size (P=0.031), lymph node metastasis (P=0.016) and TNM stage (P=0.001). Subsequently, cell viability, wound scratch, migration and invasion assays were conducted in vitro, and nude mice CRC xenograft models were generated. The results demonstrated that knockdown of PLK4 in CRC cells resulted in significant decreases in cell viability and proliferation, and decreased the protein expression levels of N‑cadherin and snail, which are biomarkers of epithelial‑mesenchymal transition. Furthermore, PLK4 knockdown inactivated the Wnt/β‑catenin pathway in CRC cells in vitro and in vivo, and suppressed the growth of xenograft tumors in nude mice. In conclusion, these results suggested that PLK4 may promote the carcinogenesis and metastasis of CRC, thus indicating that PLK4 may be considered a molecular target for CRC treatment.


Int J Oncol


Liao Z,Zhang H,Fan P,Huang Q,Dong K,Qi Y,Song J,Chen L,Liang H,Chen X,Zhang Z,Zhang B




Has Abstract


2019-02-01 00:00:00












  • gammaH2AX and cleaved PARP-1 as apoptotic markers in irradiated breast cancer BT474 cellular spheroids.

    abstract::Chemo- and radiotherapy induce apoptosis in tumours and surrounding tissues. In a search for robust and reliable apoptosis markers, we have evaluated immunostaining patterns of gammaH2AX and cleaved PARP-1 in paraffin-embedded cellular spheroids. Breast cancer BT474 cells were grown as cell spheroids to diameters of 7...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Qvarnström OF,Simonsson M,Eriksson V,Turesson I,Carlsson J

    更新日期:2009-07-01 00:00:00

  • CXCR4-transfected human umbilical cord blood-derived mesenchymal stem cells exhibit enhanced migratory capacity toward gliomas.

    abstract::Mesenchymal stem cells (MSCs) can be used as a delivery vehicle for gene therapy against brain tumors, because these cells have a migratory capacity toward glioma cells. Soluble factors including chemokines or growth factors expressed and released by glioma cells mediate the tropism of MSCs for gliomas. Among them, st...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Park SA,Ryu CH,Kim SM,Lim JY,Park SI,Jeong CH,Jun JA,Oh JH,Park SH,Oh W,Jeun SS

    更新日期:2011-01-01 00:00:00

  • Stem cells with fused gene expression of cytosine deaminase and interferon-β migrate to human gastric cancer cells and result in synergistic growth inhibition for potential therapeutic use.

    abstract::Genetically engineered stem cells (GESTECs) producing suicide enzymes and immunotherapeutic cytokines have therapeutic effects on tumors, and may possibly reduce the side effects of toxic drugs used for treatments. Suicide enzymes can convert non-toxic pro-drugs to toxic metabolites that can reduce tumor growth. Cytos...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kim KY,Yi BR,Lee HR,Kang NH,Jeung EB,Kim SU,Choi KC

    更新日期:2012-04-01 00:00:00

  • Intracellular calcium and breast-cancer cell-growth and differentiation.

    abstract::The growth of normal human breast epithelial cells in vitro, as well as those of other cell types is strongly influenced by the concentration of calcium in the culture medium [Ca++]e. The aim of this study was to ascertain if calcium also affects breast tumor cell growth in vitro. To address this question, the metasta...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Vandewalle B,Hornez L,Lassalle B,Revillion F,Bertout M,Lefebvre J

    更新日期:1993-04-01 00:00:00

  • Fibrous tumors in children - a morphologic and interphase cytogenetic analysis of problematic cases.

    abstract::We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromo...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Flores-Stadler EM,Chou PM,Barquin N,Thangavelu M,Gonzalez-Crussi F

    更新日期:2000-09-01 00:00:00

  • Tumor oxygenation and radiosensitization by pentoxifylline and a perflubron emulsion carbogen breathing.

    abstract::Oxygen tension measurements were made in three tumors: (i) the murine FSaII fibrosarcoma, (ii) the rat 9L gliosarcoma and (iii) the rat 13672 mammary adenocarcinoma using a pO2 histograph. Tumor oxygenation measurements were made while the animals breathed air or breathed carbogen (95% oxygen/5% carbon dioxide). Pento...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Teicher B,Sotomayor E,Robinson M,Dupuis N,Schwartz G,Frei E

    更新日期:1993-01-01 00:00:00

  • P-glycoprotein-associated resistance to taxol and taxotere and its reversal by dexniguldipine-HCl, dexverapamil-HCl, or cyclosporin A.

    abstract::A series of different human MDR (multidrug-resistant) cell lines including a HeLa-MDR1 transfectant which exhibit high overexpression of the MDR1/P-glycoprotein gene, but no enhanced expression of the MRP (multidrug resistance associated protein) gene, showed different ratios of relative resistances to the taxanes tax...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ise W,Heuser M,Sanders K,Beck J,Gekeler V

    更新日期:1996-05-01 00:00:00

  • Synthesis and degradation of basement membranes and extracellular matrix and their regulation by TGF-beta in invasive carcinomas (Review).

    abstract::The proper structure of the extracellular matrix, in particular of the basement membrane and the adjacent interstitial matrix, are essential prerequisites for a proper function of tissues. Invasive growth in malignant tumors is associated with a destruction of various matrix structures. Due to extensive recent analyse...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: Hagedorn HG,Bachmeier BE,Nerlich AG

    更新日期:2001-04-01 00:00:00

  • FcγR polymorphisms and clinical outcome in colorectal cancer patients receiving passive or active antibody treatment.

    abstract::Fcγ receptors (FcγRs) on effector cells are of importance for mediating antibody-dependent cellular cytotoxicity (ADCC). FcγRIIIa158valine (V)/phenylalanine (F) and FcγRIIa131histidine (H)/arginine(R) polymorphisms have been shown to relate to prognosis in antibody-treated patients. The aim of the present study was to...

    journal_title:International journal of oncology

    pub_type: 杂志文章,多中心研究


    authors: Wang B,Kokhaei P,Mellstedt H,Liljefors M

    更新日期:2010-12-01 00:00:00

  • Basal and luminal breast cancers: basic or luminous? (review).

    abstract::Three major, fast developing lines of research are elucidating the biology of the mammary gland and its malignant transformation: phenotypical analyses of epithelial cells and mammary proliferative lesions, the identification, purification and characterization of mammary stem cells and breast cancer-initiating cells, ...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: Birnbaum D,Bertucci F,Ginestier C,Tagett R,Jacquemier J,Charafe-Jauffret E

    更新日期:2004-08-01 00:00:00

  • Prognostic significance of cyclin expression in human osteosarcoma.

    abstract::To evaluate the distribution of cyclin protein expression, in relation to cell proliferation rate and clinical behavior, an immunohistochemical study was performed on 92 tumor samples of patients with high grade osteosarcoma (OS). A large cyclin A- and cyclin E-positive fraction was found respectively in 59% and 47% o...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Molendini L,Benassi MS,Magagnoli G,Merli M,Sollazzo MR,Ragazzini P,Gamberi G,Ferrari C,Balladelli A,Bacchini P,Picci P

    更新日期:1998-05-01 00:00:00

  • Chondroitin sulfate proteoglycan protein is stimulated by interleukin 11 and promotes endometrial epithelial cancer cell proliferation and migration.

    abstract::Endometrial cancer is the most common gynecological cancer. We identified interleukin 11 (IL11) as a critical mediator of endometrial tumourigenesis and demonstrated that IL11 regulates chondroitin sulfate proteoglycan (CSPG4) in human placental trophoblasts. CSPG4 is a cell membrane protein overexpressed in numerous ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Winship A,Van Sinderen M,Heffernan-Marks A,Dimitriadis E

    更新日期:2017-03-01 00:00:00

  • Radiofrequency radiation at Stockholm Central Railway Station in Sweden and some medical aspects on public exposure to RF fields.

    abstract::The Stockholm Central Railway Station in Sweden was investigated for public radiofrequency (RF) radiation exposure. The exposimeter EME Spy 200 was used to collect the RF exposure data across the railway station. The exposimeter covers 20 different radiofrequency bands from 88 to 5,850 MHz. In total 1,669 data points ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Hardell L,Koppel T,Carlberg M,Ahonen M,Hedendahl L

    更新日期:2016-10-01 00:00:00

  • Low level STK15 amplification in histologically benign urothelium of patients with bladder cancer adversely predicts patient outcome following cystectomy.

    abstract::The aim of this study was to investigate STK15 amplification in histologically benign urothelium and invasive tumor tissue of urothelial bladder cancer patients in relation to clinicopathologic and molecular characteristics, and to analyze a hypothesized association between the STK15 single nucleotide polymorphism at ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Denzinger S,Stoehr R,Schwarz S,Eichenseher N,Brockhoff G,Obermann EC,Knuechel R,Blaszyk H,Hartmann A,Wild PJ

    更新日期:2007-10-01 00:00:00

  • Icariin sensitizes human colon cancer cells to TRAIL‑induced apoptosis via ERK‑mediated upregulation of death receptors.

    abstract::Tumor necrosis factor‑associated apoptosis‑inducing ligand (TRAIL) is considered to be a potential therapeutic target for various types of cancer. However, colon cancer is difficult to treat due to its resistance to TRAIL. Therefore, various trials have been conducted to overcome TRAIL resistance in colon cancer. The ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kim B,Seo JH,Lee KY,Park B

    更新日期:2020-03-01 00:00:00

  • Oncogenic mutation of the p53 gene derived from head and neck cancer prevents cells from undergoing apoptosis after DNA damage.

    abstract::A p53 functional analysis system, which can identify the types of abnormality of p53, such as loss of function, dominant negative function, or gain of oncogenic function, is now required. In this study, we examined the functional diversity of several mutations of p53 derived from human head and neck cancer cells. The ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kawamata H,Omotehara F,Nakashiro K,Uchida D,Shinagawa Y,Tachibana M,Imai Y,Fujimori T

    更新日期:2007-05-01 00:00:00

  • Characterization of a h-3 GnRH method for the measurement of GnRH binding-sites in human breast-cancer and breast-cancer cell-lines.

    abstract::The peptide hormone, GnRH has been hypothesized to play a direct antitumoral role in certain kinds of breast cancer. This direct effect is considered to function via high affinity membrane receptors to GnRH that have been demonstrated in tumors. However, no routine assay for GnRH receptors exist limiting further resea...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Reshkin S,Pezzetta A,Albarani V,Schittulli F,Paradiso A

    更新日期:1995-08-01 00:00:00

  • DNA-hypomethylating agent, 5'-azacytidine, induces cyclooxygenase-2 expression via the PI3-kinase/Akt and extracellular signal-regulated kinase-1/2 pathways in human HT1080 fibrosarcoma cells.

    abstract::The cytosine analogue 5'-azacytidine (5'-aza) induces DNA hypomethylation by inhibiting DNA methyltransferase. In clinical trials, 5'-aza is widely used in epigenetic anticancer treatments. Accumulated evidence shows that cyclooxygenase-2 (COX-2) is overexpressed in various cancers, indicating that it may play a criti...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Yu SM,Kim SJ

    更新日期:2015-10-01 00:00:00

  • Inhibition of proliferation, activation and function of human natural-killer-cells in-vitro by secreted products of lovo, a colorectal-cancer cell-line.

    abstract::It has previously been suggested that some tumours may elaborate substances which can suppress the functional activity of lymphokine activated killer (LAK) cells. We have investigated the effects of secreted products from the colorectal cancer cell line LoVo on the pattern of expression of T lymphocyte subsets and nat...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Johnston D,Allen P,Newland A,Williams N

    更新日期:1993-10-01 00:00:00

  • Analysis of cell kinetics after gamma-ray irradiation using an anti-BrdU monoclonal antibody.

    abstract::The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrdU) monoclonal antibody and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Murata T,Akagi K,Nasu R,Kimura H,Tanaka Y

    更新日期:1998-02-01 00:00:00

  • Overexpression of multidrug resistance genes MDR1 and cMOAT in human hepatocellular carcinoma and hepatoblastoma cell lines.

    abstract::Overexpression of either of the multidrug resistance genes MDR1 and MRP is associated with resistance of tumors to multiple chemotherapeutic agents. Overexpression of MDR1 has been reported in some cell lines derived from human hepatocellular carcinomas (HCC) and hepatoblastomas (HB). The human gene for cMOAT (

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Minemura M,Tanimura H,Tabor E

    更新日期:1999-09-01 00:00:00

  • Microvessel density predicts the radiosensitivity of metastatic head and neck squamous cell carcinoma in cervical lymph nodes.

    abstract::Cervical lymph node metastasis is the most common recurrence pattern of head and neck squamous cell carcinoma (HNSCC), and it is usually treated with radiation therapy and/or neck dissection. There has long been a desire for markers useful in predicting radiosensitivity to enable assignment of patients with recurrent ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ito Y,Kamijo T,Yokose T,Kawashima M,Ogino T,Ikeda H,Hayashi R,Sasaki S,Ochiai A

    更新日期:2001-12-01 00:00:00

  • DCLK1 is correlated with MET and ERK5 expression, and associated with prognosis in malignant pleural mesothelioma.

    abstract::Malignant pleural mesothelioma (MPM) is an aggressive cancer for which more effective treatments are needed. In this study, strong to moderate staining of MET and ERK5 was detected in 67.1 and 48% of the analyzed 73 human mesothelioma tumors, and significant correlation of MET and ERK5 expression was identified (P<0.0...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Wang H,Dai YY,Zhang WQ,Hsu PC,Yang YL,Wang YC,Chan G,Au A,Xu ZD,Jiang SJ,Wang W,Jablons DM,You L

    更新日期:2017-07-01 00:00:00

  • In vitro transformation of MCF-10A cells by sera harvested from heifers two months post-Zeranol implantation.

    abstract::Among many risk factors of breast cancer, estrogens and non-estrogenic endocrine disruptors are considered to play critical roles in human breast carcinogenesis. Zeranol (Z) is a non-steroidal agent with potent estrogenic activity and has been widely used as an FDA approved beef growth promoter in the US. Recently, co...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ye W,Xu P,Zhong S,Jen R,Threlfall WR,Frasure CV,Feng E,Li H,Lin SH,Liu JY,Lin YC

    更新日期:2011-04-01 00:00:00

  • Cancer stem-like cells of glioblastoma characteristically express MMP-13 and display highly invasive activity.

    abstract::Glioblastoma is the most malignant type of primary brain tumor that has been shown to contain a small population of cancer stem cells. Recent studies have suggested that cancer stem cells cause tumor recurrence based on their resistance to radiotherapy and chemotherapy. Although the highly invasive nature of glioblast...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Inoue A,Takahashi H,Harada H,Kohno S,Ohue S,Kobayashi K,Yano H,Tanaka J,Ohnishi T

    更新日期:2010-11-01 00:00:00

  • Novel role of c-jun N-terminal kinase in regulating the initiation of cap-dependent translation.

    abstract::Initiation of protein translation by the 5' mRNA cap is a tightly regulated step in cell growth and proliferation. Aberrant activation of cap-dependent translation is a hallmark of many cancers including non-small cell lung cancer. The canonical signaling mechanisms leading to trans...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Patel MR,Sadiq AA,Jay-Dixon J,Jirakulaporn T,Jacobson BA,Farassati F,Bitterman PB,Kratzke RA

    更新日期:2012-02-01 00:00:00

  • Detection of a set of peptide vaccine candidates for use in HLA-A31+ epithelial cancer patients.

    abstract::The molecular basis of host-tumor interaction in HLA-A31+ cancer patients has not been well understood. This lack of clarification is hampering the development of specific immunotherapies for these patients. This study aimed to identify a set of CTL-epitope peptides applicable for the specific immunotherapy of cancer ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Takedatsu H,Shichijo S,Azuma K,Takedatsu H,Sata M,Itoh K

    更新日期:2004-02-01 00:00:00

  • Silencing LPAATβ inhibits tumor growth of cisplatin-resistant human osteosarcoma in vivo and in vitro.

    abstract::Cisplatin-resistance has become a major impediment in the medical treatment of cancers such as osteosarcoma, the most common primary malignancy of bone. Since lysophosphatidic acid acyltransferase β (LPAATβ) was reported to be critically involved in osteosarcoma, our study investigated the role of LPAATβ in human oste...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Song L,Duan P,Gan Y,Li P,Zhao C,Xu J,Zhang Z,Zhou Q

    更新日期:2017-02-01 00:00:00

  • Gene expression studies of hepatitis virus-induced woodchuck hepatocellular carcinoma in correlation with human results.

    abstract::The lack of good molecular markers for diagnosis as well as treatment assessment has rendered the hepatocellular carcinoma (HCC) a major challenge in health care. In this study, woodchucks were used as an animal model for hepatitis virus-induced HCC, and gene expression studies were performed using a human oligonucleo...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Wang F,Anderson PW,Salem N,Kuang Y,Tennant BC,Lee Z

    更新日期:2007-01-01 00:00:00

  • Involvement of aldehyde dehydrogenase 1A2 in the regulation of cancer stem cell properties in neuroblastoma.

    abstract::Despite the introduction of 13-cis-retinoic acid (13-cis-RA) into the current chemotherapy, more than half of high-risk neuroblastoma patients have experienced tumor relapses driven by chemoresistant cancer stem cells (CSCs) that can be isolated by their ability to grow as spheres. Although aldehyde dehydrogenase (ALD...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Hartomo TB,Van Huyen Pham T,Yamamoto N,Hirase S,Hasegawa D,Kosaka Y,Matsuo M,Hayakawa A,Takeshima Y,Iijima K,Nishio H,Nishimura N

    更新日期:2015-03-01 00:00:00