Different intracellular compartmentalization of TA and DeltaNp73 in non-small cell lung cancer.


:The p53 homologue p73 is overexpressed in many tumors, including lung cancer. We have evaluated the differential expression and subcellular localization of the functionally distinct apoptotic (TA) and anti-apoptotic (DeltaN) isoforms of p73 in non-small cell lung cancer (NSCLC), their possible association with p53 expression and determined the methylation status of the two p73 gene promoters (P1 and P2) in this tumor type. Immunohistochemical analysis showed that both isoforms are expressed in the majority of cases. However, the oncogenic DeltaN variant, derived from the transcripts DeltaN'p73 (from P1) and/or DeltaNp73 (from P2), is localized mainly in the nucleus, while the anti-oncogenic TAp73 isoform (derived from a P1 transcript) is sequestered in the cytoplasm in almost all cases analyzed. Significant correlation was found between p53 and DeltaNp73 expression (p=0.041). Methylation analysis conducted on 41 tumor samples showed that the P1 promoter is almost invariably unmethylated (39/41 cases) whereas P2 was found completely methylated in 17 cases and partially or totally unmethylated in 24 samples. No correlation was found between the methylation status of P1 and P2 and p73 expression. Our results demonstrate that both isoforms contribute to p73 overexpression in NSCLC and suggest that their different intracellular localization may reflect an alteration of the functional p53-p73 network that might contribute to lung cancer development.


Int J Oncol


Di Vinci A,Sessa F,Casciano I,Banelli B,Franzi F,Brigati C,Allemanni G,Russo P,Dominioni L,Romani M


Has Abstract


2009-02-01 00:00:00












  • Analysis of cell kinetics after gamma-ray irradiation using an anti-BrdU monoclonal antibody.

    abstract::The cell cycle was analyzed using double staining with an anti-bromodeoxyuridine (BrdU) monoclonal antibody and propidium iodide (PI). Changes in cell kinetics after irradiation were compared with those seen by the conventional PI-based DNA histogram method. The effect of irradiation on cell kinetics has been studied ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Murata T,Akagi K,Nasu R,Kimura H,Tanaka Y

    更新日期:1998-02-01 00:00:00

  • Down-regulation of FAK and IAPs by laminin during cisplatin-induced apoptosis in testicular germ cell tumors.

    abstract::The purpose of this present study was to investigate the role of inhibitor of apoptosis proteins (IAPs), their inhibitor, Smac, and the focal adhesion kinase (FAK) in the laminin enhancement of cisplatin-induced apoptosis in a testicular tumor germ cell line, NCCIT. We demonstrated that specifically in laminin-adheren...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Andjilani M,Droz JP,Benahmed M,Tabone E

    更新日期:2006-02-01 00:00:00

  • Expression of cyclin D1 proto-oncogene mRNA in primary meningiomas may contribute to tumorigenesis.

    abstract::Meningiomas are benign brain tumors thought to arise by multi-step tumorigenesis, involving both the activation of oncogenes and the loss of tumor suppressor genes. The cell cycle regulator proto-oncogene cyclin D1 has been implicated in the pathogenesis of several types of cancer. Northern blot analysis revealed expr...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Maxwell M,Galanopoulos T,Antoniades H

    更新日期:1996-12-01 00:00:00

  • Constitutive production of proinflammatory cytokines RANTES, MIP-1beta and IL-18 characterizes LGL leukemia.

    abstract::Large granular lymphocyte (LGL) leukemia is a lymphoproliferative disease often associated with autoimmune disorders such as rheumatoid arthritis. High levels of soluble Fas ligand have been implicated in development of chronic neutropenia. However, a comprehensive analysis of constitutive chemokine and lymphokine pro...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kothapalli R,Nyland SB,Kusmartseva I,Bailey RD,McKeown TM,Loughran TP Jr

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  • The role of a Drosophila replication related element in dras2 oncogene regulation.

    abstract::The Drosophila ras2 and rop gene pair are governed by a common promoter. Characterisation of the Drosophila ras2/rop bidirectional promoter has revealed that a single major protein complex (M), composed of two subunits (factors A and B) interacts with two central promoter domains (regions A and B). To define the trans...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Lightfoot K,Tai A,Segev O

    更新日期:1995-11-01 00:00:00

  • Fructose as a carbon source induces an aggressive phenotype in MDA-MB-468 breast tumor cells.

    abstract::Aberrant glycosylation is a universal feature of cancer cells, and certain glycan structures are well-known markers for tumor progression. Availability and composition of sugars in the microenvironment may affect cell glycosylation. Recent studies of human breast tumor cell lines indicate their ability to take up and ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Monzavi-Karbassi B,Hine RJ,Stanley JS,Ramani VP,Carcel-Trullols J,Whitehead TL,Kelly T,Siegel ER,Artaud C,Shaaf S,Saha R,Jousheghany F,Henry-Tillman R,Kieber-Emmons T

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  • Potentiation of cytotoxicity by combination of imatinib and chlorimipramine in glioma.

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    journal_title:International journal of oncology

    pub_type: 杂志文章


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  • Vascular endothelial growth-factor expression and angiogenesis in nonsmall cell lung carcinomas.

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    journal_title:International journal of oncology

    pub_type: 杂志文章


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    更新日期:1995-05-01 00:00:00

  • Effect of specific activity on organ uptake of iodine-123-meta-iodobenzylguanidine in humans.

    abstract::Radioiodinated meta-iodobenzylguanidine (MIBG), an analogue of norepinephrine, has been used in management of neuroendocrine tumors. Recent studies reveal that distribution of radioiodinated MIBG in animals depends on the specific activity of this radiopharmaceutical. In order to clarify the effect of specific activit...

    journal_title:International journal of oncology

    pub_type: 杂志文章


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  • Involvement of P-glycoprotein in an in vitro blood-brain barrier model.

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    journal_title:International journal of oncology

    pub_type: 杂志文章


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  • Differences in the tumor DNA analysis of a hepatocellular-carcinoma and a synchronous fibrolamellar variant.

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    journal_title:International journal of oncology

    pub_type: 杂志文章


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  • Combined effects of onconase and IFN-beta on proliferation, macromolecular syntheses and expression of STAT-1 in JCA-1 cancer cells.

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    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Tsai SY,Hsieh TC,Ardelt B,Darzynkiewicz Z,Wu JM

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  • [Corrigendum] miR-28-5p promotes the development and progression of ovarian cancer through inhibition of N4BP1.

    abstract::Following the publication of this article, the authors noted that there was an error in the affiliations. Specifically, the affiliation of the first author JUAN XU should be: Jinhua Municipal Central Hospital, Jinhua Hospital of Zhejiang University, Jinhua, Zhejiang 321000, P.R. China. [the original article was publis...

    journal_title:International journal of oncology

    pub_type: 杂志文章,已发布勘误


    authors: Xu J,Jiang N,Shi H,Zhao S,Yao S,Shen H

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  • Methylation of the CA9 promoter can modulate expression of the tumor-associated carbonic anhydrase IX in dense carcinoma cell lines.

    abstract::CA IX is a tumor-associated transmembrane isoform of the carbonic anhydrase with a high enzyme activity and a functional involvement in the pH regulation and cell adhesion. Expression of CA9 gene in tumor cells is principally regulated by the high cell density and the hypoxia-related VHL-HIF pathway. In renal cell car...

    journal_title:International journal of oncology

    pub_type: 杂志文章


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  • Two novel squamous cell carcinoma antigen-derived HLA-A*0201-binding peptides induce in vitro and in vivo CD8+ cytotoxic T lymphocyte responses.

    abstract::Squamous cell carcinoma antigen (SCCA) is overexpressed in many squamous cell cancers and SCCA‑derived peptide-specific CD8(+) cytotoxic T lymphocytes can display cytotoxicity against tumor cells. In the present study, we screened the SCCA amino acid sequence for potential HLA-A*0201-binding CD8(+) T‑cell epitopes usi...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Duan ZL,Wang ZB,Guo JL,Liu WQ,Hu J,Li J,Wang SN,Li Q,Wen JS

    更新日期:2013-04-01 00:00:00

  • Effects of herbal preparation Equiguard on hormone-responsive and hormone-refractory prostate carcinoma cells: mechanistic studies.

    abstract::The Equiguard is a dietary supplement comprised of standardized extracts from nine herbs, respectively, Herba epimedium brevicornum Maxim (stem and leaves), Radix morindae officinalis (root), Fructus rosa laevigatae michx (fruit), Rubus chingii Hu (fruit), Schisandra chinensis (Turz.) Baill (fruit), Ligustrum lucidum ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Hsieh TC,Lu X,Guo J,Xiong W,Kunicki J,Darzynkiewicz Z,Wu JM

    更新日期:2002-04-01 00:00:00

  • Therapeutic results of intra-arterial chemotherapy in patients with malignant glioma.

    abstract::Thirty-three adult patients with anaplastic astrocytoma or glioblastoma multiforme, verified between 1990 and 1997, received postoperative intra-arterial ACNU chemotherapy and irradiation. Since our previous strategies to malignant gliomas had included intra-venous ACNU, the purpose of the present study is to evaluate...

    journal_title:International journal of oncology

    pub_type: 临床试验,杂志文章


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    更新日期:1998-09-01 00:00:00

  • Novel role of c-jun N-terminal kinase in regulating the initiation of cap-dependent translation.

    abstract::Initiation of protein translation by the 5' mRNA cap is a tightly regulated step in cell growth and proliferation. Aberrant activation of cap-dependent translation is a hallmark of many cancers including non-small cell lung cancer. The canonical signaling mechanisms leading to trans...

    journal_title:International journal of oncology

    pub_type: 杂志文章


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    更新日期:2012-02-01 00:00:00

  • Nuclear morphometry for predicting cervical metastatic potential in laryngeal squamous-cell carcinoma.

    abstract::Quantitative nuclear morphometry (QNM) provides a reliable, reproducible method for measuring the degree of nuclear pleomorphism, a qualitative parameter widely incorporated into histopathological grading schemes. Nuclear shape has successfully been shown to predict primary prostate adenocarcinoma metastatic potential...

    journal_title:International journal of oncology

    pub_type: 杂志文章


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    更新日期:1995-09-01 00:00:00

  • ErbB3 expression predicts sensitivity to elisidepsin treatment: in vitro synergism with cisplatin, paclitaxel and gemcitabine in lung, breast and colon cancer cell lines.

    abstract::Irvalec® (elisidepsin trifluoroacetate, PM02734) is a novel marine-derived cyclic peptide belonging to the Kahaladide family of compounds, currently in clinical trials with preliminary evidence of antitumor activity. Previous studies have shown a correlation between elisidepsin sens...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Teixidó C,Arguelaguet E,Pons B,Aracil M,Jimeno J,Somoza R,Marés R,Ramón Y Cajal S,Hernández-Losa J

    更新日期:2012-07-01 00:00:00

  • Dexamethasone downregulates expression of carbonic anhydrase IX via HIF-1α and NF-κB-dependent mechanisms.

    abstract::Dexamethasone is a synthetic glucocorticoid frequently used to suppress side-effects of anticancer chemotherapy. In the present study, we showed that dexamethasone treatment leads to concentration-dependent downregulation of cancer-associated marker, carbonic anhydrase IX (CA IX), at the level of promoter activity, mR...

    journal_title:International journal of oncology

    pub_type: 杂志文章


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    更新日期:2016-10-01 00:00:00

  • A dynamic interplay between alternative polyadenylation and microRNA regulation: implications for cancer (Review).

    abstract::Alternative polyadenylation and microRNA regulation are both mechanisms of post-transcriptional regulation of gene expression. Alternative polyadenylation often results in mRNA isoforms with the same coding sequence but different lengths of 3' UTRs, while microRNAs regulate gene expression by binding to specific mRNA ...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: An J,Zhu X,Wang H,Jin X

    更新日期:2013-10-01 00:00:00

  • Regulation of cell-growth and apoptosis in synchronized agf cells - involvement of oncogenes and cell-cycle regulatory proteins.

    abstract::Cell synchrony was induced in AGF cells by blocking of the cell cycle at GI-S boundary with high concentrations (2 mM) of thymidine for 11 h. Prolonged arrest of cells in GI-S (15 h-20 h) induced progressive and time dependent apoptosis. Early morphological changes in cellular and nuclear morphology (blebbing) were mo...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Gazitt Y,Lavia M,Erdos G

    更新日期:1993-09-01 00:00:00

  • Multidrug resistance in cancer (review).

    abstract::The development of resistance to anticancer agents during treatment is a major obstacle in the chemotherapy of cancer. Cells expressing high levels of the P-glycoprotein exhibit a multidrug resistance phenotype. The P-glycoprotein is a membrane phosphoprotein which serves as a drug efflux pump to reduce intracellular ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Srivastava R,Srivastava A,Chochung Y

    更新日期:1996-11-01 00:00:00

  • Differentiation of human breast carcinoma cells by a novel vitamin D analog: 1alpha-hydroxyvitamin D5.

    abstract::The active metabolite of vitamin D, 1alpha,25-dihydroxyvitamin D3, can induce differentiation in breast cancer cells; however, it is hypercalcemic in vivo. Therefore, development of non-calcemic analogs of vitamin D has received considerable attention. Recently, we synthesized an analog of vitamin D [1alpha(OH)D5] tha...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Mehta RR,Bratescu L,Graves JM,Green A,Mehta RG

    更新日期:2000-01-01 00:00:00

  • Targeting the integrated networks of aggresome formation, proteasome, and autophagy potentiates ER stress‑mediated cell death in multiple myeloma cells.

    abstract::The inhibitory effects of macrolide antibiotics including clarithromycin (CAM) on autophagy flux have been reported. Although a macrolide antibiotic exhibits no cytotoxicity, its combination with bortezomib (BZ), a proteasome inhibitor, for the simultaneous blocking of the ubiquitin (Ub)‑proteasome and autophagy‑lysos...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Moriya S,Komatsu S,Yamasaki K,Kawai Y,Kokuba H,Hirota A,Che XF,Inazu M,Gotoh A,Hiramoto M,Miyazawa K

    更新日期:2015-02-01 00:00:00

  • Overexpression of lamin B1 induces mitotic catastrophe in colon cancer LoVo cells and is associated with worse clinical outcomes.

    abstract::Lamins are the major components of the nuclear lamina and play important roles in many cellular processes. The role of lamins in cancer development and progression is still unclear but it is known that reduced expression of lamin B1 has been observed in colon cancer. Thus, the aim of the present study was to elucidate...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Izdebska M,Gagat M,Grzanka A

    更新日期:2018-01-01 00:00:00

  • High incidence of high-risk HPV in benign and malignant lesions of the larynx.

    abstract::The aim of this study was to determine the prevalence of human papillomavirus (HPV) in patients with laryngeal benign lesions (LBLs) and laryngeal squamous cell carcinomas (LSCCs) using a sensitive E6/E7 type-specific PCR. Paraffin-embedded samples from LBL (n=39) and LSCC patients (n=67) were evaluated for the presen...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Duray A,Descamps G,Arafa M,Decaestecker C,Remmelink M,Sirtaine N,Ernoux-Neufcoeur P,Mutijima E,Somja J,Depuydt CE,Delvenne P,Saussez S

    更新日期:2011-07-01 00:00:00

  • EGCG inhibits recepteur d'origine nantais expression by suppressing Egr-1 in gastric cancer cells.

    abstract::Abnormal accumulation and activation of the recepteur d'origine nantais (RON) has been implicated in epithelial tumor carcinogenesis. In the present study, we examined the effect of epigallocatechin-3-gallate (EGCG), the major green tea catechin, on the induction of RON and tumor growth in human gastric cancer. EGCG i...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Park JS,Khoi PN,Joo YE,Lee YH,Lang SA,Stoeltzing O,Jung YD

    更新日期:2013-03-01 00:00:00

  • Detection of hepatitis-C virus by unbalanced polymerase-chain reaction, hybridization to synthetic oligonucleotides and capillary electrophoresis.

    abstract::Polymerase chain reaction (PCR) has been reported as one of the most efficient techniques to detect hepatitis C virus (HCV) RNA. The determination of the specificity of PCR products is usually based on 'nested' PCR, Southern blotting and hybridization of the amplified DNA to radioactive oligonucleotide probes recogniz...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Bianchi N,Mischiati C,Feriotto G,Gambari R

    更新日期:1994-04-01 00:00:00