Abstract:
:Matrix metalloproteinases (MMP) play an important role in tumor cell invasion and metastasis. We examined the expression of MMPs in hepatocellular carcinoma (HCC) to determine whether they may help indicate the progression of HCC and patient outcome. The expression of MMP-1, -2, -7, -9, and MT1-MMP were determined in 37 pairs of HCC and adjacent non-tumor tissue specimens by semiquantitative reverse transcription polymerase chain reaction (RT-PCR). The tumor to non-tumor (T/N) ratios for mRNA expression of each MMP were compared with the clinicopathological findings and two-year disease-free survival rates. Immunohistochemical analysis for MMP-1 and -9 was performed in 30 specimens. MMP-1 and -9 expression was significantly higher in tumor tissue than in non-tumor tissue (p=0.001 and 0.0078, respectively). By contrast, the expression of MMP-2 and -7 was significantly lower in tumor tissue than in non-tumor tissue (p=0. 003 and 0.03, respectively). A higher MMP-9 T/N ratio was significantly associated with small HCCs (=2 cm) and higher serum AFP values (>/=20 ng/ml) (p=0.013 and 0.028, respectively). Immunohistochemical analysis suggested that well differentiated HCC showed stronger immunoreactivity for MMP-9 than moderately differentiated HCC. MMP T/N ratio was not significantly associated with the disease-free survival rates. Overexpression of MMP-9 mRNA (T/N ratio >/=2) may be associated with the progression of small HCC (=2 cm).
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Sakamoto Y,Mafune K,Mori M,Shiraishi T,Imamura H,Mori M,Takayama T,Makuuchi Mdoi
10.3892/ijo.17.2.237keywords:
subject
Has Abstractpub_date
2000-08-01 00:00:00pages
237-43issue
2eissn
1019-6439issn
1791-2423journal_volume
17pub_type
杂志文章abstract::Cytokine expression in a tumor microenvironment can impact both host defense against the tumor and tumor cell survival. In this study, we sought to clarify whether the cytokine gene expression profile could have clinical associations with ovarian cancer. We analyzed the expression of 16 cytokine genes (IL-1α, IL-1β, I...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1533
更新日期:2012-09-01 00:00:00
abstract::Rhabdomyosarcoma (RMS) is a malignant tumour of skeletal muscle origin which includes two major histological subtypes: alveolar rhabdomyosarcoma (ARMS), the more aggressive, and embryonal rhabdomyosarcoma (ERMS). In order to establish whether the higher metastatic potential of ARMS cells may depend on differential exp...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-09-01 00:00:00
abstract::Whilst the role of microRNA‑143 (miR‑143) in myelodysplastic syndrome (MDS) remains unclear, abnormally expressed microRNA‑143 has been detected in many types of cancer tissues. In this study, we describe a cohort study for the verification of miR‑143 expression, as well as the investigation of the molecular mechanism...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4534
更新日期:2018-11-01 00:00:00
abstract::The Raf/MEK/ERK signaling cascade has been extensively studied for its roles in growth and differentiation of a variety of cell types. Confliciting evidence exists regarding the function of classical MAPK signaling with regards to the development of chemotherapeutic drug resistance; some reports describe an pro-surviv...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::We compared the responses of c-Jun/AP-1 transcriptional complexes with those of NF-kappa B, an established hypoxia-inducible transcriptional complex, in hypoxic SiHa human squamous carcinoma cells. We observed that NF-kappa B was activated rapidly, while AP-1 activation was detectable only after prolonged hypoxia. How...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.5.875
更新日期:1996-05-01 00:00:00
abstract::A431 resistant variants to epoxomicin (EXM) were established, showing 4.0-6.7 times more resistance to EXM than parental A431P. Both variants demonstrated increased expression of the beta-subunit molecules of 26S proteasome with approximately 2.5 times increased activity. In variant cells, cyclin B and P34cdc2 were ov...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::Polymerase chain reaction (PCR) has been reported as one of the most efficient techniques to detect hepatitis C virus (HCV) RNA. The determination of the specificity of PCR products is usually based on 'nested' PCR, Southern blotting and hybridization of the amplified DNA to radioactive oligonucleotide probes recogniz...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.4.903
更新日期:1994-04-01 00:00:00
abstract::The monocyte chemotactic protein 3 (MCP-3) belongs to the MCP subgroup of the CC chemokines and promotes chemotaxis of immune cells. MCP-1 is believed to play an important role in monocyte infiltration into tumor tissues; however, the relationship between tumor-infiltrating macrophage/microglia (TIM/M) and the express...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000292
更新日期:2009-06-01 00:00:00
abstract::The development of colorectal cancer (CRC) is strongly correlated with the aberrant activation of multiple intracellular signaling transduction cascades including STAT3, ERK, JNK and p38 pathways which usually function redundantly. In addition, crosstalk between these pathways forms a complicated signaling network tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2040
更新日期:2013-10-01 00:00:00
abstract::Protein kinase C (PKC) designates a family of kinases that regulate many essential functions including cell growth and differentiation. The tight regulation of PKC activity is crucial for maintaining normal cellular proliferation and excessive activity leads to abnormal or uncontrolled cell growth. Recent reports indi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.2.327
更新日期:1999-02-01 00:00:00
abstract::MicroRNAs (miRNAs) are small non-coding, endogenous, single-stranded RNAs. MiRNAs have been implicated in different areas such as the immune response, neural development, DNA repair, apoptosis, oxidative stress response and cancer. However, while the majority of miRNAs are found int...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2012.1647
更新日期:2012-12-01 00:00:00
abstract::Long non-coding RNAs (lncRNAs) are involved in cancer progression. In the present study, we analyzed the lncRNA profiles in adriamycin-resistant and -sensitive breast cancer cells and found a group of dysregulated lncRNAs in the adriamycin-resistant cells. Expression of the dysregulated lncRNAs was correlated with dys...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3665
更新日期:2016-10-01 00:00:00
abstract::alpha-tocopheryl phosphate (TocP) has been elucidated for diverse effectiveness through unequivocal mechanisms independent of its conversion to alpha-tocopherol (Toc). The present study showed that TocP, in a dose-dependent manner, repressed invasion of human fibrosarcoma HT-1080 cells through the basement membrane, m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000445
更新日期:2009-12-01 00:00:00
abstract::With a view to identifying markers reflecting not the evidence of, but the potential for, neoplastic progression of the breast we have compared the normal invasive mammary epithelial cell growth seen at puberty with invasive and non-invasive carcinogenesis using the mouse as a model. We have analyzed cell proliferatio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Metastatic disease commonly occurs in the spine and incidence is likely to increase secondary to improved survival rates in many cancer patients. Despite published research on instability in patients with metastatic disease of the thoracolumbar spine, controversy exists regarding risk factors for instability and indic...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2011-01-01 00:00:00
abstract::Overexpression of either of the multidrug resistance genes MDR1 and MRP is associated with resistance of tumors to multiple chemotherapeutic agents. Overexpression of MDR1 has been reported in some cell lines derived from human hepatocellular carcinomas (HCC) and hepatoblastomas (HB). The human gene for cMOAT ( journal_title:International journal of oncology pub_type: 杂志文章 doi:10.3892/ijo.15.3.559 更新日期:1999-09-01 00:00:00
abstract::Loss of chromosome sequences at 13q14 (Rbl) and 17p13 (p53) associated with squamous cell carcinoma of head and neck (SCCHN) was evaluated in 12 recurrent tumors and 51 primary tumors from 63 patients. The incidence of LOH at 17p13 was 19 of 50 (38%) tumors, and at 13q14 was 21 of 57 (37%). LOH affecting Rbl and/or p5...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.6.1235
更新日期:1997-06-01 00:00:00
abstract::Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many types of cell lines and tissues have demonstrated the presence of SP cells, including colon cancer cell lines. This study aimed to identify cancer stem cells (CSCs) in the SP of the colon cancer cell line SW480. SP c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2498
更新日期:2014-09-01 00:00:00
abstract::Cytogenetic analyses of peripheral blood cells of breast cancer patients unveiled in one case a grossly enlarged G-band in one copy of chromosome 16 (16qh+), which scored also strongly positive in C-banding. Fluorescence in situ hybridization with both a total chromosome 16 library and with probe pHuR195 detecting loc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.2.347
更新日期:1994-02-01 00:00:00
abstract::Aurora-A is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. Aurora-A is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. However, how these different binding signals work together to regulate Auro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000691
更新日期:2010-08-01 00:00:00
abstract::We performed this study in order to evaluate the impact of the chemokine CXCL12 and its single-nucleotide polymorphism (SNP) rs1801157 on clinicopathological parameters and survival in patients undergoing surgery for esophagogastric cancer. The expression pattern of CXCL12 and its polymorphisms were analyzed by RT-PCR...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1044
更新日期:2011-08-01 00:00:00
abstract::CD34 is commonly used as an endothelial cell marker of tumor vessels. However, this marker detects not only newly formed, but also pre-existing large blood vessels. Nestin, a class VI intermediate filament protein, has recently received attention as a marker for detecting newly formed endothelial cells. In this study,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-03-01 00:00:00
abstract::CDC28 protein kinase regulatory subunit 2 (CKS2) is a cyclin-dependent kinase subunit (CKS) family member that participates in cell cycle regulation. Few studies have investigated its involvement in gastric cancer. In this study, we focused on the clinical and biological significance of CKS2 over-expression in gastric...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1056
更新日期:2011-08-01 00:00:00
abstract::Most cancer cells trigger thrombin generation (TG) to various extent. In the present study, we dissected the mechanisms responsible for the procoagulant activity of pancreatic adenocarcinoma cells (BXPC3), a highly thrombogenic cancer type, and breast cancer cells (MCF7), a less thombogenic tumor type. TG of normal pl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4172
更新日期:2017-12-01 00:00:00
abstract::Currently, it is not entirely clear whether hypoxia-inducible factor-1α (HIF-1α) is involved in the regulation of COX-2 expression and epithelial-to-mesenchymal transition (EMT), and whether these events affect the prognosis of hepatocellular carcinoma (HCC) patients treated with transcatheter arterial chemoembolizati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3421
更新日期:2016-05-01 00:00:00
abstract::Large granular lymphocyte (LGL) leukemia is a lymphoproliferative disease often associated with autoimmune disorders such as rheumatoid arthritis. High levels of soluble Fas ligand have been implicated in development of chronic neutropenia. However, a comprehensive analysis of constitutive chemokine and lymphokine pro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
abstract::In this study tumor-specific CTL effector and memory responses were analyzed in normal mice, in tumor bearing mice and in animals treated by active specific immunotherapy (ASI) with an autologous virus modified tumor vaccine. In the well defined DBA/2 mouse lymphoma model ESb, the tumor specific CTL response requires ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.2.217
更新日期:1999-08-01 00:00:00
abstract::Clinical trials using antiangiogenic drugs revealed their potential against cancer. Unfortunately, a large percentage of patients does not yet benefit from this therapeutic approach highlighting the need of diagnostic tools to non-invasively evaluate and monitor response to therapy. It would also allow to predict whic...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2016.3553
更新日期:2016-08-01 00:00:00
abstract::Transforming growth factor-β receptor II (TGFBR2) is implicated in various types of cancer. Most molecules involved in the TGF-β pathway can be degraded by one or more microRNAs (miRNAs). In the present study, we show that miRNA plays an important role in downregulating TGFBR2 expression in Ewing's sarcoma (ES) cells....
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4155
更新日期:2017-12-01 00:00:00
abstract::Dose is a critical determinant of the efficacy of chemotherapy. The dose response curve for virtually all anticancer agents is steep in both in vitro and in vivo experimental studies. However the application of the steep dose-response curve in the clinic has been troublesome for many years because of dose limiting bon...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00