Abstract:
:Polymerase chain reaction (PCR) has been reported as one of the most efficient techniques to detect hepatitis C virus (HCV) RNA. The determination of the specificity of PCR products is usually based on 'nested' PCR, Southern blotting and hybridization of the amplified DNA to radioactive oligonucleotide probes recognizing sequences comprised between the PCR primers. The recent introduction of capillary electrophoresis (CE) to analyse DNA fragments and PCR products appears to be very interesting because this technology is rapid, reproducible, sensitive and could be suitable to detect DNA/DNA and DNA/RNA hybrids. We demonstrate that specific hybridization of an HCV oligonucleotide probe to single stranded HCV-DNA obtained by unbalanced PCR is detectable by capillary electrophoresis, therefore enabling a one-step, non-radioactive protocol to demonstrate the specificity of amplification of HCV sequences by PCR.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Bianchi N,Mischiati C,Feriotto G,Gambari Rdoi
10.3892/ijo.4.4.903subject
Has Abstractpub_date
1994-04-01 00:00:00pages
903-7issue
4eissn
1019-6439issn
1791-2423journal_volume
4pub_type
杂志文章abstract::Induction of differentiation represents a promising concept for chemotherapy of malignant gliomas, which are often refractory even to the combined treatment with surgery, irradiation and chemotherapy. Since anti-neoplastic alkylphosphocholines can induce differentiation of leukemic cell lines, the effects of the intra...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.4.673
更新日期:2001-10-01 00:00:00
abstract::Inositol hexaphosphate (IP6), also known as phytic acid, has been shown to exhibit anticancer effects in a number of preclinical tumor models. IP6 decreases proliferation by arresting cells in the G0/G1 phase, inhibits iron-mediated oxidative reactions, enhances differentiation and stimulates apoptosis. The present st...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4488
更新日期:2018-10-01 00:00:00
abstract::Increasing evidence has confirmed that dysregulation of microRNAs (miRNAs) can contribute to the progression and metastasis of human tumors. Previous studied have shown dysregulation of miR-24 in a variety of tumors. However, the roles of miR-24 in human bladder cancer have not been well clarified. Therefore, we inves...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3117
更新日期:2015-10-01 00:00:00
abstract::Peripheral blood mononulear cells (PBMC) derived from 10 asymptomatic human T lymphotropic virus type I (HTLV-I) carriers were cultured for a short term (10-14 days) in the absence of exogenous antigens. In 5 carriers, when compared with 5 HTLV-I non-carriers an apparent increase in the proportion of CD8+ DR+ cells wa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.2.159
更新日期:1994-08-01 00:00:00
abstract::p73 has been shown to transcriptionally activate genes positively responsive to wild-type p53. In order to undertake a comparative study of functions of p53 and p73 we have cloned the cDNA of p73 from MCF-7 cells. Adenovirus onco-protein E1A inhibits the transactivation by p73; a deletion mutant of E1A incapable of in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-02-01 00:00:00
abstract::Acquisition of P-gp-mediated multidrug-resistance does not always correlate with observed malignant behavior of NB. To characterize alterations accompanying development of multidrug-resistance in NB we established two neuroblastoma cell sublines resistant to vincristine (UKF-NB-3rVCR10) and doxorubicin (UKF-NB-3rDOX20...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-10-01 00:00:00
abstract::Retinoids, which include vitamin A (retinol) and its derivatives, have previously been investigated as potential chemopreventive and chemotherapeutic agents in bladder cancer. We examined mRNA expression of the retinoid receptors RARalpha, RARbeta2, RARgamma and RXRalpha, as well as two putative RARbeta2 target genes,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::MicroRNA (miRNA or miR) inhibition of oncogenic related pathways has been shown to be a promising therapeutic approach for cancer. SIRT1 might be a promoter factor on tumorigenesis of hepatocellular carcinoma (HCC). However, the mechanism is unknown. We investigated whether miRNAs regulate the SIRT1 and its downstream...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2596
更新日期:2014-11-01 00:00:00
abstract::CDH13 (H-cadherin) is a member of the cadherin superfamily, which plays an important role in cell recognition and adhesion. We examined the expression and methylation status of the CDH13 gene in diffuse large B cell lymphomas (B-DLCLs). We found decreased expression of the CDH13 gene in all of 6 hematopoietic cell lin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract::We investigated the clinical significance of the expression levels of matrix metalloproteinase 9 (MMP-9) in renal cell carcinoma (RCC). In addition, we validated the efficacy of pyrrole imidazole polyamide (PIP) targeting MMP-9 on inhibiting proliferation and invasion of RCC. We evaluated the expression levels of MMP-...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2073
更新日期:2013-11-01 00:00:00
abstract::Primary intracerebral lymphoma is an uncommon presenting site for non-Hodgkin's lymphoma. The authors review 28 histopathologically confirmed, consecutive cases, presenting over a 15-year period. The cohort included 20 males and 8 females with a mean age at diagnosis of 54 years (range 27-75 years). Subtotal resection...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.4.647
更新日期:1999-04-01 00:00:00
abstract::Prognosis of patients with undifferentiated gastric cancer is generally poor. The expression of various microRNAs (miRNAs) has not been comprehensively investigated in undifferentiated gastric cancer. Total RNA was extracted from the specimens of 42 undifferentiated gastric cancer tissues and paired normal gastric tis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2009-02-01 00:00:00
abstract::TGF-beta1 stimulates proliferation of WI38 human embryo fibroblasts but inhibits that of their SV40-transformed counterparts, VA13 cells. Protein expression levels of cyclins A, D1, E and that of cdk2 and cdk4 were not affected by TGF-beta1 in either of these cells. However, TGF-beta1-treatment increases cdk2 kinase a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.5.905
更新日期:1999-05-01 00:00:00
abstract::The aim of the present study was to evaluate the performance of whole-body diffusion-weighted imaging (WB-DWI), whole-body positron emission tomography with computed tomography (WB-PET/CT), and whole-body positron emission tomography with magnetic resonance imaging (WB-PET/MRI) in staging patients with untreated invas...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4012
更新日期:2017-07-01 00:00:00
abstract::Enhanced Notch signaling contributes to uncontrolled cell growth and cell death resistance in cancer. Here, we demonstrate that in colorectal carcinoma cells the Notch1-dependent activation of cell cycle and proliferation is mediated by repression of the cyclin-dependent kinase inhibitor (CDKI) p27. The half-life of p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.2140
更新日期:2013-12-01 00:00:00
abstract::The human prostate gland is a well known source of H1 and H2 relaxin but information is lacking on the expression and potential role of the INSL3 peptide hormone within the prostate gland. In the present study we have investigated the expression of human INSL3 in patients with benign prostate hyperplasia (BPH), prosta...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::No population-based study has been conducted on the relationship between chemotherapy and the risk of cognitive impairments in patients with colorectal cancer. This study aimed to determine this association in a large population-based cohort of patients. We studied 72,374 men and women who were diagnosed with stages I...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1882
更新日期:2013-06-01 00:00:00
abstract::One hundred and eighty-one breast cancer specimens were analyzed for nuclear p53 staining by immunochemical methods. There were 123 fine-needle cytological specimens and 58 frozen tissue sections of surgical biopsies. The microscopic evaluation of the staining fitted with a 4 group classification. Ninety-one samples (...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.4.747
更新日期:1997-04-01 00:00:00
abstract::A herbal preparation denoted PC SPES(R) is available in 'natural food' or 'health food' stores in the United States. This mixture (patent pending, US Serial number 08/697,920) consists of extracts from 8 different herbs, 7 originating from China and one from America, and is sold as a dietary supplement. Although sever...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.3.437
更新日期:1997-09-01 00:00:00
abstract::Cordycepin (3'-deoxyadenosine), a major bioactive compound of Cordyceps militaris, has many pharmacological actions, such as anti-inflammatory and anticancer activities. In this study, the relationship between inhibition of cell motility and anti-invasive activity by cordycepin in L...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1332
更新日期:2012-05-01 00:00:00
abstract::Neuroblastoma (NB) is the most common extra-cranial solid tumor in children and despite aggressive therapy survival rates remain low. One of the contributing factors for low survival rates is aggressive tumor angiogenesis, which is known to increase due to radiation, one of the standard therapies for neuroblastoma. Th...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3646
更新日期:2016-10-01 00:00:00
abstract::Among many risk factors of breast cancer, estrogens and non-estrogenic endocrine disruptors are considered to play critical roles in human breast carcinogenesis. Zeranol (Z) is a non-steroidal agent with potent estrogenic activity and has been widely used as an FDA approved beef growth promoter in the US. Recently, co...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.941
更新日期:2011-04-01 00:00:00
abstract::Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage of As2O3 in cervical cancer and its possible mechanisms, human cervical cancer cell line HeLa was employed. The cells underwent apopt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-07-01 00:00:00
abstract::Lisofylline, a dimethylxanthine derivative, has been shown to block the induction of hematopoietic growth inhibitors produced in response to cytotoxic anticancer therapy. In the current study, mice bearing established EMT-6 mammary carcinoma were treated with high dose cyclophosphamide, melphalan, BCNU, 5-fluorouracil...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.2.265
更新日期:1997-08-01 00:00:00
abstract::Aurora-A is involved in chromosome alignment, centrosome maturation, mitotic spindle assembly and regards to an oncogene. Aurora-A is also known to bind to several other proteins affecting its up-regulation or down-regulation and localization. However, how these different binding signals work together to regulate Auro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000691
更新日期:2010-08-01 00:00:00
abstract::Because K-Ras mutation and cyclooxygenase-2 (COX-2) overexpression are hallmarks of majority of pancreatic cancer patients, an approach to inhibit the progression and growth of pancreatic cancer using the simultaneous administration of agents that inhibit the function of both targets, should be considered. In the pres...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2350
更新日期:2014-06-01 00:00:00
abstract::Bile duct carcinoma patients generally have a poor prognosis. Understanding this cancer at the biological, genetic, molecular, and cellular level in ways relevant to clinical management is essential for developing effective preventive and therapeutic regimens. However, the currently established bile duct cancer cell l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-02-01 00:00:00
abstract::Thymidine kinase (TK) activity in rat hepatoma JB1 and AH136B was mainly due to cytosolic TK and was much higher than that in rat regenerating liver. Two forms of cytosolic TK, designated as TK-I and TK-II, were revealed in addition to mitochondrial TK in anion-exchange high performance liquid chromatography (HPLC) of...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1999-08-01 00:00:00
abstract::Sirtuin 6 (SIRT6) is a member of the sirtuin deacetylases (sirtuins), which are derivatives of the yeast Silent information regulator 2 (Sir2) protein. SIR2 and its mammalian derivatives play a central role in epigenetic gene silencing, recombination, metabolism, cell differentiation and in the regulation of aging. In...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-02-01 00:00:00
abstract::Hypoxia, a common phenomenon during the development of malignant solid tumors including breast cancer, serves to propagate a cascade of molecular pathways triggered by hypoxia-inducible factor-1α (HIF-1α). Carbonic anhydrase IX (CA IX), one of the target genes of HIF-1α, has been reported to be involved in progression...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3253
更新日期:2016-01-01 00:00:00