Abstract：
:Sirtuin 6 (SIRT6) is a member of the sirtuin deacetylases (sirtuins), which are derivatives of the yeast Silent information regulator 2 (Sir2) protein. SIR2 and its mammalian derivatives play a central role in epigenetic gene silencing, recombination, metabolism, cell differentiation and in the regulation of aging. In contrast to most sirtuins, SIRT6 lacks NAD+-dependent protein deacetylase activity. We have isolated and characterized the human Sirt6 genomic sequence, which spans a region of 8,427 bp and which has one single genomic locus. Determination of the exon-intron splice junctions found the full-length SIRT6 protein to consist of 8 exons ranging in size from 60 bp (exon 4) to 838 bp (exon 8). The human Sirt6 open reading frame encodes a 355-aa protein with a predictive molecular weight of 39.1 kDa and an isoelectric point of 9.12. Characterization of the 5' flanking genomic region, which precedes the Sirt6 open reading frame, revealed a TATA- and CCAAT-box less promoter with an approximately 300-bp long CpG island. A number of AML-1 and GATA-x transcription factor binding sites were found which remain to be further evaluated experimentally. Fluorescence in situ hybridization analysis localized the human Sirt6 gene to chromosome 19p13.3; a region which is frequently affected by chromosomal alterations in acute leukemia. Human SIRT6 appears to be most predominantly expressed in bone cells and in the ovaries while, in the bone marrow, it is practically absent. The functional characteristics of SIRT6 are essentially unknown at present and remain to be elucidated.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Mahlknecht U,Ho AD,Voelter-Mahlknecht Skeywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
447-56issue
2eissn
1019-6439issn
1791-2423journal_volume
28pub_type
杂志文章abstract：:Toxicity of polyphenols against rat 3Y1 fibroblasts and the cells transformed by human adenovirus (Ad12-3Y1), its EIA gene (EIA-3Y1), or simian virus 40 (SV-3Y1) was examined. Among the diphenol compounds examined, pyrocatechol (o-diphenol) and hydroquinone (p-diphenol) showed selective toxicity against Ad12-3Y1 and E...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2.1.89
更新日期：1993-01-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is one of the most malignant types of cancer, and is associated with high recurrence rates and a poor response to chemotherapy. Immune signatures in the microenvironment of HCC have not been well explored systematically. The aim of the present study was to identify prognostic immune sign...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2020.4998
更新日期：2020-05-01 00:00:00
abstract：:We investigated three clonally related human keratinocyte cell lines of different biological behaviour, HaCaT (non-tumorigenic), A5 (benign, tumorigenic) and II-4RT (malignant, tumorigenic), with regard to the cell-associated localization of the major MMPs -1, -2, -3, -9, -10 and -11. These observations were correlate...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2002-03-01 00:00:00
abstract：:Hepatocellular carcinoma (HCC) is known to be resistant to chemotherapy. Survivin, a member of the inhibitor of apoptosis proteins, is overexpressed in most cancers but is absent in most normal adult tissue. The aim of this study was to investigate whether expression of survivin contributes to resistance to cisplatin-...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2010-07-01 00:00:00
abstract：:Thymidylate synthase (TS) is an essential enzyme for DNA biosynthesis that is an important target of fluoropyrimidine anti-cancer drugs such as 5-fluorouracil (5-FU), which is frequently used against human colon adenocarcinomas. Because levels of TS in such tumors may be predictive of chemotherapeutic response to 5-FU...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：1993-02-01 00:00:00
abstract：:There is increasing evidence that different types of breast cancers are related to distinct risk factors. We analyzed the risk of breast cancer with respect to circulating insulin-like growth factor (IGF)-I, IGF-binding protein (IGFBP)-3, 17beta-estradiol, estrone, testosterone, androstenedione and sex hormone-binding...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2006-07-01 00:00:00
abstract：:Long noncoding RNA (lncRNA) MAF BZIP transcription factor G antisense RNA 1 (MAFG‑AS1) has been demonstrated to serve an important role in the progression of various types of cancer, whereas its role in breast cancer has not been fully elucidated. The present study aimed to explore the potential role and underlying me...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2020.5150
更新日期：2020-11-20 00:00:00
abstract：:Tamoxifen (Tam), a selective estrogen receptor modulator, is in wide clinical use for the treatment and prevention of breast cancer. However, extended TAM administration for breast cancer induces increased VEGF levels in patients, promoting new blood vessel formation and thereby limiting its efficacy and highlighting ...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2014.2299
更新日期：2014-04-01 00:00:00
abstract：:Gap junctional intercellular communication (GJIC) has been shown to be involved in the bystander effect through herpes simplex virus thymidine kinase/ganciclovir (HSV-tk/GCV) gene therapy. In this study, we examined the expression of connexins, the components of gap junction, and the degree of GJIC in esophageal cance...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:We have previously reported that Insulin-like growth factor (IGF-I) is a cellular growth factor of human transformed T-lymphocytes. To evaluate the role of IGF-I for nontransformed T-cells we investigated synthesis of IGF-I and the influence of an IGF-I antiserum, UBK487, during proliferation process. IGF-I is synthes...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2.6.1003
更新日期：1993-06-01 00:00:00
abstract：:We have examined six patients with multiple low grade, low stage superficial multifocal bladder tumors with surrounding tissues for loss of heterozygosity (LOH) and microsatellite instability at chromosome 3, totaling 76 samples. The majority (4/5) of the patients had LOH at or close to the fragile histidine triad (FH...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.18.1.203
更新日期：2001-01-01 00:00:00
abstract：:Peptide hormone relaxin produced in normal and cancer prostate cells can stimulate prostate cancer progression. Suppression of relaxin or relaxin receptor RXFP1 expression inhibited prostate cancer both in vitro and in vivo. RXFP1 is a G protein-coupled receptor with the extracellular low density lipoprotein A (LDL-A)...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2011.1159
更新日期：2011-12-01 00:00:00
abstract：:Cancer stem cells (CSCs), which have the potential for self-renewal, differentiation and de-differentiation, undergo epigenetic, epithelial-mesenchymal, immunological and metabolic reprogramming to adapt to the tumor microenvironment and survive host defense or therapeutic insults. Intra-tumor heterogeneity and cancer...
journal_title：International journal of oncology
pub_type： 杂志文章,评审
doi：10.3892/ijo.2017.4129
更新日期：2017-11-01 00:00:00
abstract：:Interleukin-2 (IL-2) which has no cross-resistance with chemotherapy, has given responses in tumors resistant to chemotherapy, and shown to be more effective when tumor burden is low. 13-cis retinoic acid (RA) has immunomodulatory properties, potentially synergistic with IL-2. The objective of this pilot phase II stud...
journal_title：International journal of oncology
pub_type： 临床试验,杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:With a view to identifying markers reflecting not the evidence of, but the potential for, neoplastic progression of the breast we have compared the normal invasive mammary epithelial cell growth seen at puberty with invasive and non-invasive carcinogenesis using the mouse as a model. We have analyzed cell proliferatio...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:The platinum complex trans-[PtCl2¿E-HN=C(OMe)Me¿2] was compared to cisplatin for cytotoxicity towards tumour cells, and for cellular pharmacological properties in A2780 and cisplatin-resistant A2780/Cp8 ovarian cancer cells. Trans-[PtCl2¿E-HN=C(OMe)Me¿2] was comparably cytotoxic to cisplatin (mean IC50 after 72 h expo...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.15.5.1039
更新日期：1999-11-01 00:00:00
abstract：:To evaluate the significance of microsatellite instability (MI) and loss of heterozygosity (LOH) in the development of different histological subgroups of liposarcomas, we examined 28 tissue-samples from 21 patients and the corresponding non-neoplastic reference tissues. We investigated nine microsatellite loci and de...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.14.4.721
更新日期：1999-04-01 00:00:00
abstract：:A total of forty-one tumor infiltrating T cell lines (TIL) were cloned, in the presence of interleukin-2, from nine breast tumor and five melanoma specimens with limiting dilution in a microculture system. Nineteen (46%) of the lines/clones reacted to autologous tumor targets. The T cell receptor (TcR) V beta gene usa...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.7.1.147
更新日期：1995-07-01 00:00:00
abstract：:BALB/c 3T3 cells transformed by 1,1,2,2-tetrachloroethane, the most toxic chloroethane, acquired a fully malignant phenotype. They were capable of growing in soft agar, were tumorigenic when injected in athymic mice and produced pulmonary nodules after i.v. injection. Untreated BALB/c 3T3 cells were weakly tumorigenic...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2.6.937
更新日期：1993-06-01 00:00:00
abstract：:Cell synchrony was induced in AGF cells by blocking of the cell cycle at GI-S boundary with high concentrations (2 mM) of thymidine for 11 h. Prolonged arrest of cells in GI-S (15 h-20 h) induced progressive and time dependent apoptosis. Early morphological changes in cellular and nuclear morphology (blebbing) were mo...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：1993-09-01 00:00:00
abstract：:Cordycepin is the main functional component of Cordyceps militaris, which has been widely used in oriental traditional medicine. This compound has been shown to possess many pharmacological properties, such as enhancing the body's immune function, and anti-inflammatory, anti-aging and anticancer effects. In the presen...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2013.1762
更新日期：2013-03-01 00:00:00
abstract：:Gene amplification is a frequent event in lung cancer, specifically in squamous cell lung carcinoma. Recently, we reported amplifications on chromosomal bands 3q26.1-q26.3 with the genes BCHE and SLC2A2 amplified in 40% of squamous cell lung carcinomas. Here, we identified an amplified domain within chromosomal bands ...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.17.1.67
更新日期：2000-07-01 00:00:00
abstract：:We have previously demonstrated the multipotent nature of human umbilical cord blood stem cells (hUCB). In this study, we have attempted to show the use of hUCB in glioma therapy. We used hUCB enriched in CD44 and CD133 cells for our studies and observed that glioma cells co-cultured with hUCB undergo apoptosis. To pr...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo_00000599
更新日期：2010-05-01 00:00:00
abstract：:The purpose of this study was to determine, using fluorescence in situ hybridization (FISH), whether treatment of cancer cells with 3'-azido-2'-3'-dideoxythymidine (AZT) has any effect on telomere length as determined by the telomeric signal intensity. To do so, we treated a metastatic murine melanoma cell line (K-173...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.13.5.923
更新日期：1998-11-01 00:00:00
abstract：:Serial analysis of gene expression (SAGE) is a sequence-based tool that enables quantitative study of comprehensive gene expression profiles. In this study, we generated a SAGE library of hepatitis B virus (HBV)-associated small hepatocellular carcinoma (HCC) and compared it with libraries of normal liver and other or...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo_00000321
更新日期：2009-07-01 00:00:00
abstract：:Human telomerase reverse transcriptase (hTERT), the catalytic subunit of telomerase, is very closely associated with telomerase activity. Telomerase has been implicated in cellular immortalization and carcinogenesis. In situ detection of hTERT will aid in determining the localization of telomerase-positive cells. The ...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:The aim of the present study was to investigate the effects of lysyl oxidase‑like 2 (LOXL2) on the invasion, migration and epithelial‑to‑mesenchymal transition (EMT) of renal cell carcinoma (RCC) cells through the steroid receptor coactivator (Src)/focal adhesion kinase (FAK) signaling pathway. RCC tissues and adjacen...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2019.4726
更新日期：2019-05-01 00:00:00
abstract：:Fanconi anemia complementation group D2 (FANCD2) is involved in the key steps of the Fanconi anemia (FA) pathway, which plays a role in the repair of DNA crosslink damage. However, the role of FANCD2 during radiotherapy for head and neck squamous cell carcinoma (HNSCC) is unclear. In this study, the HNSCC cell line HS...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2017.3902
更新日期：2017-03-07 00:00:00
abstract：:Human chorionic gonadotropin (hCG) inhibits the growth of human breast epithelial cells MCF-7. Differential display was utilized for analyzing RNA of hCG-treated MCF-7 cells in order to determine whether this effect was mediated by specific gene activation or repression. Three cDNA clones, 19, 29 and 44, were differen...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：1998-09-01 00:00:00
abstract：:The aim of this study was to investigate STK15 amplification in histologically benign urothelium and invasive tumor tissue of urothelial bladder cancer patients in relation to clinicopathologic and molecular characteristics, and to analyze a hypothesized association between the STK15 single nucleotide polymorphism at ...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2007-10-01 00:00:00
