Abstract:
:The P-glycoprotein (P-gp) multidrug transporter is present at the luminal face of the brain capillary endothelial cells that contribute to the blood-brain barrier. To study its role in transendothelial anticancer drug transport, we made use of a co-culture system formed of bovine brain capillary endothelial cells and astrocytes which allows the in vitro maintenance of specialized properties of the brain endothelial cells, including expression of P-gp as assessed by Northern and Western blot analyses. Vinblastine, an anticancer drug substrate for P-gp and known not to enter the brain, was found to be poorly transferred across the endothelial cell monolayer. This low vinblastine transport was however strongly increased in the presence of verapamil, a well known P-gp blocker. Moreover, verapamil was shown to increase the accumulation of the anticancer drug in the brain endothelial cells through inhibition of drug efflux. These results suggest that P-gp activity evidenced in the co-culture model is involved in the low transendothelial transport of vinblastine, thus supporting the conclusion that P-gp expressed at the blood-brain barrier level may prevent xenobiotics, including anticancer drugs, from entering the central nervous system.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Joly B,Lecureur V,Puozzo C,Guillouzo A,Fardel Osubject
Has Abstractpub_date
1996-11-01 00:00:00pages
1029-33issue
5eissn
1019-6439issn
1791-2423journal_volume
9pub_type
杂志文章abstract::The overall goal of the present study was to evaluate the chemotherapeutic and cancer‑protective properties of D‑erythro‑sphingosine (sphingosine) and C2‑ceramide using a human breast epithelial cell (HBEC) culture system, which represents multiple‑stages of breast carcinogenesis. The HBEC model includes Type I HBECs ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4641
更新日期:2019-02-01 00:00:00
abstract::To clarify the relationship between angiogenesis and the early development of colon cancer, expression of platelet-derived endothelial cell growth factor (PD-ECGF), a known angiogenic and endothelial cell chemotactic factor, was examined in 119 human colon premalignant adenomas and colon carcinomas. Localization of PD...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.6.1105
更新日期:1996-06-01 00:00:00
abstract::Employing the transgenic BALB-neuT mouse tumor model, we explored the in vivo biologic relevance of immunocompetent epitopes shared among the four ErbB receptors. The outcome of neu-mediated tumorigenesis was compared following vaccination with isogeneic normal rat ErbB2/Neu (LTR-Neu) or xenogeneic human ErbB receptor...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-02-01 00:00:00
abstract::The purpose of the study was to determine whether the organ environment can influence the response of colon cancer cells to chemotherapy. The highly metastatic human colon cancer cell line KM12L4, previously selected for production of liver metastases in nude mice, was injected into the cecal wall and into the spleen ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.3.413
更新日期:1993-09-01 00:00:00
abstract::Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3643
更新日期:2016-10-01 00:00:00
abstract::We have reported that ONO-4007, a novel synthetic lipid A derivative with low toxic activities, produced large amount of tumor necrosis factor (TNF)-alpha selectively in the tumor tissues and brought about complete cures in about 60% of rats bearing TNF-alpha sensitive KDH-8 cells, which also secreted a large amount o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::To compare the oncosuppressive activity of integrated and episomal adeno-associated virus (AAV), two Epstein-Barr virus (EBV) derived episomal form vectors, p205 and p220, were used to generate plasmids containing episomal AAV. HeLa cells transfected with p205, p220, and the plasmids with forward (pA205-1, pA220-1) an...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1999-07-01 00:00:00
abstract::An ETS family member, ETS Related Gene (ERG) is involved in the Ewing family of tumors as well as leukemias. Rearrangement of the ERG gene with the TMPRSS2 gene has been identified in the majority of prostate cancer patients. Additionally, overexpression of ERG is associated with unfavorable prognosis in prostate canc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1014
更新日期:2011-07-01 00:00:00
abstract::The naturally occurring photosensitizer, hypericin, with its high quantum yield of singlet oxygen photogeneration was studied for its ability to differentiate between glioblastoma cells and fetal rat neurons using fluorescence microscopy. Eight human glioma cell lines and twelve primary human glioma cell cultures were...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Paclitaxel (taxol) has been used for the treatment of various human tumors and is an exceedingly efficient chemotherapy agent against esophageal cancer. However, the precise molecular mechanisms of paclitaxel effects on human esophageal adenocarcinoma cells are not well understood. MTT assay and cell cycle analysis we...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1135
更新日期:2011-12-01 00:00:00
abstract::The objective of these studies was to examine the murine pharmacokinetics, pharmacodynamics and metabolism of (3-(1H-indol-2-yl)phenyl)(1H-indol-2-yl)methanone (Indole 15), a novel tubulin inhibitor for the treatment of cancer. We developed HPLC and LC/MS/MS assays to quantitate Indole 15 and characterize its metaboli...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1442
更新日期:2012-07-01 00:00:00
abstract::A series of different human MDR (multidrug-resistant) cell lines including a HeLa-MDR1 transfectant which exhibit high overexpression of the MDR1/P-glycoprotein gene, but no enhanced expression of the MRP (multidrug resistance associated protein) gene, showed different ratios of relative resistances to the taxanes tax...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.5.951
更新日期:1996-05-01 00:00:00
abstract::Side population (SP) cells may play a crucial role in tumorigenesis and the recurrence of cancer. Many types of cell lines and tissues have demonstrated the presence of SP cells, including colon cancer cell lines. This study aimed to identify cancer stem cells (CSCs) in the SP of the colon cancer cell line SW480. SP c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2498
更新日期:2014-09-01 00:00:00
abstract::Prostate cancer is initially sensitive to hormone therapy; however, over time the majority of patients progress to a hormone-insensitive form classified as castration-resistant prostate cancer (CRPC). CRPC is highly metastatic and patients have a poor prognosis. Thus, new drugs for the treatment of this disease are re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4207
更新日期:2018-02-01 00:00:00
abstract::Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to induce apoptosis in a variety of cancer cells, including colon, prostate, breast and leukemia. Among them, aspirin, a classical NSAID, shows promise in cancer therapy in certain types of cancers. We hypothesized that aspirin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1304
更新日期:2012-04-01 00:00:00
abstract::Irvalec® (elisidepsin trifluoroacetate, PM02734) is a novel marine-derived cyclic peptide belonging to the Kahaladide family of compounds, currently in clinical trials with preliminary evidence of antitumor activity. Previous studies have shown a correlation between elisidepsin sens...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1425
更新日期:2012-07-01 00:00:00
abstract::Dual oxidase 2 (Duox2), one of the seven members of the NADPH oxidase gene family, plays a critical role in generating H2O2 for thyroid hormone biosynthesis and as an integral part of the host defense system of the respiratory epithelium and the gastrointestinal tract. Recent evidence suggests that the regulation of D...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1821
更新日期:2013-04-01 00:00:00
abstract::Chronic myeloid leukemia (CML) is a malignant hematopoietic disorder distinguished by the presence of a BCR‑ABL1 fused oncogene with constitutive kinase activity. Targeted CML therapy by specific tyrosine kinase inhibitors (TKIs) leads to a marked improvement in the survival of the patients and their quality of life. ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5163
更新日期:2020-12-23 00:00:00
abstract::Sialyl Le(a) antigen (CA19-9), a member of a family of high molecular weight glycoproteins, was originally described as a gastrointestinal- and pancreatic-specific tumor marker. Recent studies have demonstrated that sialyl Le(a) is a ligand for E-selectin and may play an important role in tumor metastasis. However, ex...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::Surfactant proteins play important roles in lung surfactant function and innate immunity. The DNA methylation state of 11 CpG sites of surfactant protein (SP)-A1, -B, -C, and -D was determined using universal bead arrays. A total of 90 cancerous and non-cancerous tissues from 23 patients with adenocarcinoma and 22 wit...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::Osteosarcoma is the most common primary malignancy of the bone. There have been some advances in surgical and chemotherapeutic strategies, but it is still a tumor with a high mortality rate in children and young adults. Mitogen-activated protein kinase/extracellular signal regulated...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1303
更新日期:2012-04-01 00:00:00
abstract::Tissue samples from a malignant melanoma of the anal canal and its local metastasis were short-term cultured and analyzed cytogenetically. Complex chromosome aberrations were found in the primary tumor, yielding the karyotype 49-50,X,-Y,der(1)t(1;13)(q44;q12),+der(2)t(2;8) (q31;q12),der(6)t(5;6)(q13;q21),+del(6)(q12q2...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.3.555
更新日期:1995-03-01 00:00:00
abstract::Due to the central role in predicting response to herceptin and possibly also other anticancer drugs, accurate and reproducible detection of the HER2 status is important. Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are the most commonly used methods for HER2 analysis. It is a disadvantage ...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::In this study we investigated the relationship between the development of either HSILs (high-grade squamous intraepithelial lesions) or invasive cancers of the uterine cervix and the p53 codon 72 polymorphisms consisting of arginine (Arg)- or proline (Pro)-encoded allele in Japanese populations. Furthermore, we determ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Double-stranded RNA-dependent protein kinase (PKR) is one of the players in the cellular antiviral responses and is involved in transcriptional stimulation through activation of NF-κB. Treatment of the human osteosarcoma cell line MG63 with the protein phosphatase inhibitor okadaic acid stimulated the expression and p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1911
更新日期:2013-06-01 00:00:00
abstract::Breast cancer cells can switch from estrogen receptor α (ER)- to human epidermal growth factor receptor (HER)-driven cell growth upon acquiring antiestrogen resistance. HER ligands are cleaved by metalloproteinases leading to release of active HER ligands, activation of HER receptors and consequently increased cell gr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2434
更新日期:2014-07-01 00:00:00
abstract::Total body irradiation (TBI), given in low to moderate doses as a single modality, can enhance leukocyte populations and immune modifiers, resulting in slowed tumor progression. The aim of this study was to evaluate natural killer (NK) cell involvement in mediating the antitumor effect of TBI by depleting NK populatio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::Metformin is a commonly used oral anti-hyperglycemic agent of the biguanide family. Recent studies suggest that metformin may reduce cancer risk and improve prognosis. However, the antitumor mechanism of metformin in several types of cancers, including hepatocellular carcinoma (HCC), has not been elucidated. The goal ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2419
更新日期:2014-07-01 00:00:00
abstract::Chemokine-mediated activation of G protein-coupled receptors CXCR1/2 promotes tumor growth, invasion, inflammation and metastasis. Repertaxin, a CXCR1/2 small-molecule inhibitor, has been shown to attenuate many of these tumor-associated processes. The present study aimed to investigate the effects of repertaxin alone...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3371
更新日期:2016-04-01 00:00:00
abstract::We have demonstrated anti-proliferation and anti-metastasis effects of both interferon-alpha and a histone deacetylase inhibitor, sodium butyrate, on human liver cancer cell lines. In this study, invasive ability of human liver cancer cell lines through the matrix-coated membrane was examined and inhibitory effect of ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-04-01 00:00:00