Abstract:
:A series of different human MDR (multidrug-resistant) cell lines including a HeLa-MDR1 transfectant which exhibit high overexpression of the MDR1/P-glycoprotein gene, but no enhanced expression of the MRP (multidrug resistance associated protein) gene, showed different ratios of relative resistances to the taxanes taxol and taxotere. Using these cell lines the chemosensitizing efficacies of several structurally different chemosensitizers, i.e. the dihydropyridine dexniguldipine-HC1 (B8509-035), its main pyridine metabolite M1 (B8909-008), the cyclic peptide cyclosporin A, or the phenylalkylamine dexverapamil-HCl, were examined applying a 72 h tetrazolium based colorimetric MTT-assay, or a 96 h sulforhodamine B assay. Remarkably, we observed in some instances that the modulating efficacy of a particular chemosensitizer was strongly dependent on the cell line used for experimentation. Thus, dexniguldipine-HCl efficiently modulated taxane resistances of the ovarian carcinoma MDR cell line 2780AD in the submicromolar concentration range, whereas cyclosporin A and the other chemosensitizers were rather ineffective. Dexniguldipine-HCl or cyclosporin A, however, both showed a similarly strong modulating activity on the HeLa-MDR1 transfectant in clear contrast to the effects observed using the pyridine B8909-008, or dexverapamil-HCl, respectively, at the same final concentrations. Our results point to additional, as yet unidentified factors beyond the expression levels of P-glycoprotein which could contribute to the susceptibility of MDR cells to a combined treatment using taxanes and different chemosensitizing compounds. This result appears to be important considering the clinical application of chemosensitizers for combination therapy of tumors of different origin.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Ise W,Heuser M,Sanders K,Beck J,Gekeler Vdoi
10.3892/ijo.8.5.951subject
Has Abstractpub_date
1996-05-01 00:00:00pages
951-6issue
5eissn
1019-6439issn
1791-2423journal_volume
8pub_type
杂志文章abstract::In an attempt to isolate potential molecular targets for diagnosis, treatment and/or prevention of colorectal cancer (CRC), we have been analyzing expression profiles of clinical samples from CRC patients using genome-wide cDNA microarray. Among the genes up-regulated frequently in colorectal tumors, we here focused o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-08-01 00:00:00
abstract::Postoperative adjuvant chemotherapy reportedly improves advanced colorectal cancer patients' survival, however, it is necessary to assess what regimens are useful. Doxifluridine (5'-DFUR) is an intermediate of capecitabine approved in Europe and USA to treat metastatic colorectal cancer. 5'-DFUR is metabolized to 5-fl...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
doi:
更新日期:2003-07-01 00:00:00
abstract::We studied the expresssion of estrogen (ER), progesterone (PgR), and androgen receptors (AR) in hepatocellular carcinoma (HCC) and found expression in (7/36), 3.2% (1/31), 16.7% (4/24) of cases, respectively. The expression of ER did not correlate with histological grade, tumor size, or stage of disease. On the other ...
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journal_title:International journal of oncology
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abstract::Cholecystokinin (CCK) and gastrin stimulate growth of pancreatic cancer. Although down-regulation of gastrin inhibits growth of pancreatic cancer, the contribution of endogenous CCK to tumor growth is unknown. The purpose of this study was to evaluate the role of endogenous CCK on autocrine growth of pancreatic cancer...
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pub_type: 杂志文章
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更新日期:2020-03-01 00:00:00
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journal_title:International journal of oncology
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更新日期:2002-07-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-01-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:2008-08-01 00:00:00
abstract::Carcinoma‑associated fibroblasts (CAFs) secrete factors that increase the expression and/or activities of proteins in breast cancer cells and induce resistance to anti‑estrogens, such as fulvestrant. A major factor is interleukin‑6 (IL‑6). This study demonstrated that, across estrogen receptor (ER)α‑positive and ‑nega...
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pub_type: 杂志文章
doi:10.3892/ijo.2019.4918
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4207
更新日期:2018-02-01 00:00:00
abstract::The purpose of the study was to determine whether the organ environment can influence the response of colon cancer cells to chemotherapy. The highly metastatic human colon cancer cell line KM12L4, previously selected for production of liver metastases in nude mice, was injected into the cecal wall and into the spleen ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.3.413
更新日期:1993-09-01 00:00:00
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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doi:10.3892/ijo.2017.3983
更新日期:2017-06-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
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pub_type: 杂志文章
doi:10.3892/ijo.1.3.265
更新日期:1992-08-01 00:00:00
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journal_title:International journal of oncology
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journal_title:International journal of oncology
pub_type: 杂志文章,评审
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journal_title:International journal of oncology
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更新日期:1996-09-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00
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journal_title:International journal of oncology
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pub_type: 杂志文章
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pub_type: 杂志文章
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