Abstract:
:Mesothelioma is an aggressive tumor which is mainly derived from the pleura of lung. In the present study, we evaluated the anticancer effect of suberoylanilide hydroxamic acid (SAHA), a histone deacetylase (HDAC) inhibitor on human mesothelioma cells in relation to the levels of HDAC1, reactive oxygen species (ROS) and thioredoxin (Trx). While 1 µM SAHA inhibited cell growth in Phi and ROB cells at 24 h, it did not affect the growth in ADA and Mill cells. Notably, the level of HDAC1 was relatively overexpressed among Phi, REN and ROB cells. SAHA induced necrosis and apoptosis, which was accompanied by the cleavages of PARP and caspase-3 in Phi cells. This agent also increased the loss of mitochondrial membrane potential (MMP, ΔΨm) in Phi cells. All the tested caspase inhibitors attenuated apoptosis in SAHA-treated Phi cells whereas HDAC1 siRNA enhanced the apoptotic cell death. SAHA increased intracellular ROS levels including O2•- in Phi cells. N-acetyl cysteine (NAC) and vitamin C (Vit.C) significantly reduced the growth inhibition and death of Phi cells caused by SAHA. This drug decreased the mRNA and protein levels of Trx1 in Phi and ROB cells. Furthermore, Trx1 siRNA increased cell death and O2•- level in SAHA-treated Phi cells. In conclusion, SAHA selectively inhibited the growth of Phi and ROB mesothelioma cells, which showed the higher basal level of HDAC1. SAHA-induced Phi cell death was related to oxidative stress and Trx1 levels.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
You BR,Park WHdoi
10.3892/ijo.2016.3402subject
Has Abstractpub_date
2016-05-01 00:00:00pages
2197-204issue
5eissn
1019-6439issn
1791-2423journal_volume
48pub_type
杂志文章abstract::Human marrow-derived mesenchymal stem cells (MSC), which have the potential to differentiate into mesenchymal tissues, such as bone, cartilage, adipose and bone marrow stroma, were transduced with a retroviral vector carrying the simian virus 40 large T antigen, hygromycin-resistant gene and herpes simplex virus thymi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-10-01 00:00:00
abstract::A series of different human MDR (multidrug-resistant) cell lines including a HeLa-MDR1 transfectant which exhibit high overexpression of the MDR1/P-glycoprotein gene, but no enhanced expression of the MRP (multidrug resistance associated protein) gene, showed different ratios of relative resistances to the taxanes tax...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.5.951
更新日期:1996-05-01 00:00:00
abstract::Transforming growth factor-β receptor II (TGFBR2) is implicated in various types of cancer. Most molecules involved in the TGF-β pathway can be degraded by one or more microRNAs (miRNAs). In the present study, we show that miRNA plays an important role in downregulating TGFBR2 expression in Ewing's sarcoma (ES) cells....
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4155
更新日期:2017-12-01 00:00:00
abstract::Expression and functions of interleukin (IL)-8, a pro-inflammatory cytokine with angiogenesis action, was examined in 23 surgically resected hepatocellular carcinoma (HCC) specimens and 7 HCC cell lines. In all HCC tissues, IL-8 expression was confirmed with reverse-transcription polymerase chain reaction method and e...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.2.257
更新日期:2001-02-01 00:00:00
abstract::Colorectal cancer (CRC) treatment primarily relies on chemotherapy along with surgery, radiotherapy and, more recently, targeted therapy at the late stages. However, chemotherapeutic drugs have high cytotoxicity, and the similarity between the effects of these drugs on cancerous and healthy cells limits their wider us...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4536
更新日期:2018-11-01 00:00:00
abstract::Renal cell carcinoma (RCC) is resistant to chemo-therapy partly due to the overexpression of the P-glycoprotein. Several tumor suppressor genes have been reported to be silenced by hypermethylation of the promoter region in RCC. We recently reported that the in vitro cytotoxicity of vinblastine (VBL) was enhanced by p...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.999
更新日期:2011-06-01 00:00:00
abstract::Metastatic disease commonly occurs in the spine and incidence is likely to increase secondary to improved survival rates in many cancer patients. Despite published research on instability in patients with metastatic disease of the thoracolumbar spine, controversy exists regarding risk factors for instability and indic...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2011-01-01 00:00:00
abstract::The inhibitor of oxidative phosphorylation, 2,4-dinitrophenol (DNP), abrogates etoposide cytotoxicity in murine leukemia L1210 cells without affecting the quantity of drug-induced DNA lesions. Cell cycle arrest and events associated with cell death were followed in etoposide treated L1210 cells under conditions in whi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.2.305
更新日期:1996-02-01 00:00:00
abstract::A murine B16 melanoma parental line (B16) and two clones either pigmented (B16P) or non-pigmented (B16NP) were cocultured as aggregates with mouse 3T3 fibroblasts. In vivo, these mixed aggregates developed more aggressive tumors in the mouse than corresponding pure aggregates. In vitro, the three pure cell lines secre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.5.1125
更新日期:1994-11-01 00:00:00
abstract::Chronic administration of 17 beta-estradiol to male Syrian hamsters for at least six months induces estrogen-dependent kidney tumors, which express high levels of c-fos mRNA compared to surrounding renal or control tissues. We have investigated the cellular localization of c-Fos oncoprotein in these tissues and studie...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.3.527
更新日期:1995-09-01 00:00:00
abstract::The regions upstream from the human N-myc gene were examined for both transcription and replication. The plasmids containing several N-myc regions linked to the bacterial chloramphenicol acetyltransferase (CAT) gene were transfected to human neuroblastoma IMR32 cells. The results indicated that the region spanning fro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.3.603
更新日期:1994-09-01 00:00:00
abstract::We have previously demonstrated the multipotent nature of human umbilical cord blood stem cells (hUCB). In this study, we have attempted to show the use of hUCB in glioma therapy. We used hUCB enriched in CD44 and CD133 cells for our studies and observed that glioma cells co-cultured with hUCB undergo apoptosis. To pr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000599
更新日期:2010-05-01 00:00:00
abstract::Cancer antigens RAK-p120, p42, and p25, which exhibit biological, immunological and molecular similarity to the proteins expressed by Human Immunodeficiency Virus 1 (HIV-1), were found in 47 of 47 tested cases of serous adenocarcinoma of the ovary, and 45 of 45 tested cases of squamous carcinoma of the cervix. Normal ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.9.4.693
更新日期:1996-10-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one the most common malignancies and has poor prognosis in patients. The aim of the present study is to explore the clinical significance of the main genes involved in the Janus kinase (JAK)‑signal transducer and activator of transcription (STAT) pathway in HCC. GSE14520, a training c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4862
更新日期:2019-10-01 00:00:00
abstract::Endometrial cancer (EC) is one of the most common malignancies of the female reproductive system, and metastasis is a major cause of mortality. In this study, we aimed to explore the role of CP‑31398 in the migration, invasion and apoptosis of EC cells by its regulation of the expression of the murine double minute 2 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4681
更新日期:2019-03-01 00:00:00
abstract::Solid tumors can generate a plethora of neurogenesis-related molecules that enhance their growth and metastasis. Among them, we have identified axonal guidance molecule Semaphorin 7A (SEMA7A) in breast cancer. The goal of this study was to determine the therapeutic effect of suppressing SEMA7A levels in the 4T1 murine...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4144
更新日期:2017-11-01 00:00:00
abstract::Multiple myeloma (MM) patients initially respond to conventional therapy, however, many develop resistance and have recurrences. We have reported in other tumors that the transcription factor Yin Yang 1 (YY1) is a resistant factor and, thus, we hypothesized that YY1 may be over-expressed in MM. Significantly, higher e...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2511
更新日期:2014-09-01 00:00:00
abstract::We included in a case-control study on brain tumours and mobile and cordless telephones 1,617 patients aged 20-80 years of both sexes diagnosed during January 1, 1997 to June 30, 2000. They were alive at the study time and had histopathology verified brain tumour. One matched control to each case was selected from the...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-02-01 00:00:00
abstract::Macrophages are a major component of the leukocyte infiltrate of tumors and play a pivotal role in the progression of hepatocellular carcinoma (HCC). However, the molecular mechanisms by which macrophages promote HCC invasion are poorly understood. The present study was undertaken to investigate the relationship betwe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2761
更新日期:2015-02-01 00:00:00
abstract::Malignant astrocytomas are highly invasive, vascular neoplasms that comprise the majority of nervous system tumors in humans. A strong association has previously been made between malignancy in human astrocytic tumors and increased expression of certain fibroblast growth factor (FGF) family members. MG-160 is an intri...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-05-01 00:00:00
abstract::A431 resistant variants to epoxomicin (EXM) were established, showing 4.0-6.7 times more resistance to EXM than parental A431P. Both variants demonstrated increased expression of the beta-subunit molecules of 26S proteasome with approximately 2.5 times increased activity. In variant cells, cyclin B and P34cdc2 were ov...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-02-01 00:00:00
abstract::The prognosis of non-small cell lung cancer (NSCLC) patients who failed two or more treatment regimens remains very poor. We conducted a phase II study to explore the feasibility of personalized peptide vaccination (PPV), in which peptides are selected and administered based on the pre-existing host immunity before va...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2699
更新日期:2015-01-01 00:00:00
abstract::Cervical cancer is a potentially preventable disease; however, it is the third most commonly diagnosed cancer and the fourth leading cause of cancer deaths in women worldwide. Cervical cancer is thought to develop through a multistep process involving virus, tumor suppressor genes, proto-oncogenes and immunological fa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2873
更新日期:2015-04-01 00:00:00
abstract::Macrophages activated by lipopolysaccharide or interferon-gamma have been shown to be cytotoxic to tumor cells by releasing nitric oxide. Here, we report that unstimulated rat alveolar macrophages cultured with certain tumor cells produce nitric oxide and are cytotoxic to these tumor cells. Alveolar macrophages were t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.6.1053
更新日期:1993-06-01 00:00:00
abstract::Metal-containing compounds have been extensively studied for many years as potent proteasome inhibitors. The 20S proteasome, the main component of the ubiquitin proteasome pathway, is one of the excellent targets in anticancer drug development. We recently reported that several copper complexes were able to inhibit ca...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3542
更新日期:2016-08-01 00:00:00
abstract::The promising benefits of cancer gene therapy have been limited by the inability to deliver therapeutic genes homogeneously throughout the tumor mass and to control gene expression within the tumor cells. Transcriptional targeting, the use of DNA regulatory promoter sequences to localize transgene expression, has been...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2004-03-01 00:00:00
abstract::The aberrant expression of p53 and RB proteins was investigated by immunohistochemistry in 73 patients with esophageal SCC, who underwent esophageal resection. Fresh tumor tissues from 21 patients were also tested for p53 mutations in exons 4 through 9 by direct sequencing. Positive nuclear staining with p53 antibody ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.5.1109
更新日期:1997-11-01 00:00:00
abstract::Hsp90α is a molecular chaperone protein involved in the structural maturation of oncogenic signaling proteins. Hsp90 was recently identified as an anticancer target; various studies are ongoing to find ways for managing cancer through Hsp90α. However, this approach is limited by reported side-effects. Hypoxia is a hal...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1450
更新日期:2012-07-01 00:00:00
abstract::Lumican is a member of the small leucine-rich proteoglycan (SLRP) family and participates in the maintenance of tissue structures and tumor growth. Neuroendocrine cell tumors (NETs) including carcinoid tumors and NE cell carcinomas (NECs) possess numerous neuroendocrine (NE) granules, and differences between these tum...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-04-01 00:00:00
abstract::In the multistep progressive pathogenesis of human breast cancer, comedo ductal carcinoma in situ (DCIS) represents a preinvasive precursor lesion for therapy resistant invasive cancer. Human tissue derived cell culture models exhibiting molecular similarities to clinical DCIS facilitate an important preclinical mecha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-10-01 00:00:00