Abstract:
:Due to the central role in predicting response to herceptin and possibly also other anticancer drugs, accurate and reproducible detection of the HER2 status is important. Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) are the most commonly used methods for HER2 analysis. It is a disadvantage of FISH that a fraction of cases remain not interpretable probably due to suboptimal tissue handling before analysis. To investigate a possible influence of tissue damage on the results of HER2 IHC we compared the HER2 IHC results obtained in tumors with and without interpretable FISH in a breast cancer tissue microarray. The HER2 IHC results differed greatly between 1551 tumors with interpretable HER2 FISH signals and 405 breast cancers showing no FISH signals. FISH informative tumors had an IHC score of 3+ in 12.6%, 2+ in 3% and 1+ in 9.2% of cases. FISH non-informative tumors showed significantly lower IHC scores (p < 0.0001). They were IHC 3+ in 3.9%, 2+ in 3.7% and 1+ in 4.4% of cases. Overall, the data show that not only FISH but also IHC results are dependent on good tissue quality for successful analysis. Poor tissue quality can be easily identified in FISH analyses because of a lack of hybridization signals. Inappropriate tissue handling is more dangerous in IHC because an artificial lack of staining can be regarded as 'negative' result.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Tapia C,Schraml P,Simon R,Al-Kuraya KS,Maurer R,Mirlacher M,Novotny H,Spichtin H,Mihatsch MJ,Sauter Gkeywords:
subject
Has Abstractpub_date
2004-12-01 00:00:00pages
1551-7issue
6eissn
1019-6439issn
1791-2423journal_volume
25pub_type
临床试验,杂志文章abstract::Although most hepatocellular carcinoma (HCC) is related to viral infection, there is a substantial population of HCC patients (5-20%) who are negative for both markers of hepatitis B virus and hepatitis C virus infection [non-B, non-C (NBNC) hepatitis] in Japan and the incidence of NBNC-HCC has recently tended to incr...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2013.2061
更新日期:2013-11-01 00:00:00
abstract::Previous studies have revealed that protein kinase C (PKC) is responsible for malignant progression. In the present study, we investigated the potent inhibitory effects of an antiestrogen, toremifene, on PKC-mediated cellular adhesion. A phorbol ester, phorbol 12-myristate 13-acetate (PMA), significantly enhanced alph...
journal_title:International journal of oncology
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doi:10.3892/ijo.16.6.1159
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pub_type: 杂志文章
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::RhKGF is developed for prevention and treatment of chemotherapy- and radiation-induced epithelial damage in cancer patients. Little information exists on growth modulation of malignant cells by KGF. We have tested the anchorage-independent clonal growth of 35 human tumor and 22 lymphoma and leukemia cell lines in semi...
journal_title:International journal of oncology
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doi:
更新日期:2004-10-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1994-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:1995-01-01 00:00:00
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abstract::We have previously investigated the possibility of using hypericin as a diagnostic tool for the fluorescence detection of flat bladder carcinoma. In these clinical studies, it was shown that following intravesical application in humans, hypericin becomes selectively localized in transitional papillary carcinoma and ca...
journal_title:International journal of oncology
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