Abstract：
:As epidemiological data suggest ultraviolet (UV) radiation to be the major environmental factor for the development of melanoma, we screened this malignancy for UV-specific C right curved arrow T and CC right curved arrow TT DNA mutations as described in squamous and basal cell carcinomas of the skin. Mitochondrial (mt) DNA was addressed which is known to be highly more susceptible for mutations than nuclear DNA. In the mt genome we chose part of the non-coding displacement-loop (D-loop) containing two hypermutable (C)n tracts especially prone for C right curved arrow T and CC right curved arrow TT changes. A total of 69 melanoma resection specimens was investigated by polymerase chain reaction (PCR) and single-strand conformation polymorphism (SSCP) electrophoresis, followed by DNA cloning and sequencing. We detected alterations of the mt D-loop fragment in 4/34 (12%) melanoma primary tumours and in 7/35 (20%) cutaneous and subcutaneous metastases which was no statistically higher proportion upon Fisher's exact test (p=0.17). Among these 11 positive cases, 9 exhibited alterations in the (C)n microsatellite sequences indicating microsatellite instability (MSI) of mtDNA (C)n tracts. Besides 7 insertions and 2 deletions of nucleotides, 11 mutations occurred including only 4 UV-specific C right curved arrow T mutations in a nodular melanoma of the skin and in two subcutaneous metastases. CC right curved arrow TT mutations were not detected in any tissue sample. As (C)n tract alterations occurred in 2/9 primary melanomas with subsequent metastasis, whereas all 20 non-metastasizing cutaneous melanomas were not affected, these somatic changes may reflect genomic imbalance during tumour progression and may be useful for the assessment of patients' prognosis.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Deichmann M,Kahle B,Benner A,Thome M,Helmke B,Näher Hkeywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
137-41issue
1eissn
1019-6439issn
1791-2423journal_volume
24pub_type
杂志文章abstract：:Following the publication of this article, an interested reader drew to our attention that Fig. 1C contained an important flaw. The Figure shows a western blot for LCN2, DR4, DR5, and actin, and it was noted that the identical bands shown for actin were also featured in a paper by the same authors published in 2017 [L...
journal_title：International journal of oncology
pub_type： 杂志文章,已发布勘误
doi：10.3892/ijo.2019.4748
更新日期：2019-05-01 00:00:00
abstract：:The suppressors of cytokine signaling (SOCS) family inhibits not only Janus kinase (JAK)/signal transducers and activators of transcription (STAT) but also focal adhesion kinase (FAK) signaling pathways, and has tumor suppressor activity. Aberrant methylation in the promoter region of the SOCS3 gene frequently occurs ...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2007-03-01 00:00:00
abstract：:Malignant peripheral nerve sheath tumors (MPNSTs) are aggressive soft tissue tumors arising sporadically although more frequently in patients with Neurofibromatosis type 1. Prognosis remains dismal as chemo- and radiotherapy have not been shown to be successful. The heparin-binding growth factor, Midkine (MK), is impl...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2005-11-01 00:00:00
abstract：:Neuroblastoma is a paediatric solid tumor characterized by recurrent genomic abnormalities of prognostic importance. One of the most commonly observed abnormalities is deletion of the short arm of chromosome 1 and reduced expression of cancer related genes in this chromosomal arm. The long isoform of the retinoblastom...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo_00000784
更新日期：2010-11-01 00:00:00
abstract：:The presence of cancer stem cells in both solid and hematopoietic malignancies, has been recently linked to their pathogenesis. Sarcomas are rare, and diversely characterized by degrees of mesenchymal differentiation. The aim of the current study was to demonstrate whether the human sarcoma cell lines, osteosarcoma MG...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2009-05-01 00:00:00
abstract：:Prognosis of patients with undifferentiated gastric cancer is generally poor. The expression of various microRNAs (miRNAs) has not been comprehensively investigated in undifferentiated gastric cancer. Total RNA was extracted from the specimens of 42 undifferentiated gastric cancer tissues and paired normal gastric tis...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2009-02-01 00:00:00
abstract：:Colorectal cancer (CRC) treatment primarily relies on chemotherapy along with surgery, radiotherapy and, more recently, targeted therapy at the late stages. However, chemotherapeutic drugs have high cytotoxicity, and the similarity between the effects of these drugs on cancerous and healthy cells limits their wider us...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2018.4536
更新日期：2018-11-01 00:00:00
abstract：:We have previously demonstrated the multipotent nature of human umbilical cord blood stem cells (hUCB). In this study, we have attempted to show the use of hUCB in glioma therapy. We used hUCB enriched in CD44 and CD133 cells for our studies and observed that glioma cells co-cultured with hUCB undergo apoptosis. To pr...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo_00000599
更新日期：2010-05-01 00:00:00
abstract：:Glutathione S-transferase (GST) enzymes catalyse the detoxification of by-products of reactive oxygen species and are thus important in cellular defence mechanisms. The GSTs are polymorphic with allelic variants encoding isoforms with functional differences. GST polymorphism has been associated with susceptibility and...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2006-01-01 00:00:00
abstract：:Baicalein is a flavonoid derived originally from the root of Scutellaria baicalensis Georgi, which has been used in Oriental medicines for treating various diseases. Although this compound has been reported to have anticancer activities in several human cancer cell lines, the therapeutic effects of baicalein on human ...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2016.3606
更新日期：2016-09-01 00:00:00
abstract：:The present in vitro study on three human renal cell carcinoma (RCC) cell lines (A-498, ACHN, SN12C) evaluated the efficacy of 2',2'-difluorodeoxycytidine (dFdC, gemcitabine), vinblastine (VBL), rhu-interferon-alpha (IFN-alpha) and rhu-interferon-gamma (IFN-gamma) alone or in combinations. The cytotoxicity was measure...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.12.6.1361
更新日期：1998-06-01 00:00:00
abstract：:Cytogenetic abnormalities are the hallmarks of most cancers and cutaneous melanomas are no exception. The purpose of this study was to determine whether nonrandom chromosomal abnormalities are associated with development and spontaneous regression of congenital cutaneous melanoma in Sinclair miniature swine. Careful a...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.11.1.53
更新日期：1997-07-01 00:00:00
abstract：:The in situ formation of cytotoxic metabolites by an enzyme-catalyzed reaction is a recent approach in cancer therapy. The present results show that multidrug-resistant human colon adenocarcinoma cells (LoVo) are significantly more sensitive than corresponding wild-type cells to hydrogen peroxide and aldehydes, the pr...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2006-10-01 00:00:00
abstract：:Metastasis is an important prognosis factor in lung cancer, therefore, it is imperative to identify target molecules and elucidate molecular mechanism of metastasis for developing new therapeutics and diagnosis methods. We searched for metastasis-related proteins by utilizing a novel antibody proteome technology devel...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2015.3000
更新日期：2015-07-01 00:00:00
abstract：:Mesenchymal stem cells (MSCs) can be used as a delivery vehicle for gene therapy against brain tumors, because these cells have a migratory capacity toward glioma cells. Soluble factors including chemokines or growth factors expressed and released by glioma cells mediate the tropism of MSCs for gliomas. Among them, st...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2011-01-01 00:00:00
abstract：:Thirty-four children with newly diagnosed acute nonlymphoblastic leukemia were analysed for the expression of P-glycoprotein (P-170), glutathione S-transferase-pi, (GST-pi) topoisomerase II (Topo II) and the proliferation antigen Ki-67 by the streptavidin-biotin-peroxidase method. Overexpression of P-170 was present i...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.5.4.841
更新日期：1994-10-01 00:00:00
abstract：:Whilst the role of microRNA‑143 (miR‑143) in myelodysplastic syndrome (MDS) remains unclear, abnormally expressed microRNA‑143 has been detected in many types of cancer tissues. In this study, we describe a cohort study for the verification of miR‑143 expression, as well as the investigation of the molecular mechanism...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2018.4534
更新日期：2018-11-01 00:00:00
abstract：:Side population (SP) cells are isolated from various tissues and cell lines based on the exclusion of DNA-binding dye Hoechst 33,342 and exhibit potent stem cell characteristics. There have been few previous reports of SP cells in head and neck cancer cell lines. Thus, we isolated SP cells from oral squamous cell carc...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2009-08-01 00:00:00
abstract：:Lymph node metastasis of tumors is a crucial early step in the metastatic process. Tumor lymphangiogenesis plays an important role in promoting tumor metastasis to regional lymph nodes. Norcantharidin (NCTD) has been reported to possess potent anti-angiogenesis and antitumor propert...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2012.1603
更新日期：2012-11-01 00:00:00
abstract：:Cervical cancer constitutes the second most common cancer in women. It is evident from earlier studies that epidermal growth factor (EGF) is a mitogen, in that it mimics the function of estrogen by mediating cross-talk with other oncoproteins. Although epidermal growth factor recept...
journal_title：International journal of oncology
pub_type： 杂志文章,收录出版
doi：10.3892/ijo.2011.1211
更新日期：2012-01-01 00:00:00
abstract：:The poor prognosis of Ewing's sarcoma (EWS), together with its high lethal recurrence rate and the side‑effects of current treatments, call for novel targeted therapies with greater curative effectiveness and substantially reduced side‑effects. The oncogenic chimeric protein EWS/FLI1 is the key malignancy driver in mo...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2013.2016
更新日期：2013-09-01 00:00:00
abstract：:This study was designed to identify epigenetically regulated genes and to clarify the contribution of epige-netic alteration to acquired resistance to epidermal growth factor receptor‑tyrosine kinase inhibitors (EGFR‑TKIs). We established a gefitinib‑resistant lung cancer cell line, PC9, which was originally gefitinib...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2014.2733
更新日期：2015-01-01 00:00:00
abstract：:Mechanisms leading to inhibitory effects of an anti-GD2 ganglioside (GD2) 14G2a mouse monoclonal antibody (mAb) and PI3K/Akt/mTOR pathway inhibitors on human neuroblastoma cell survival were studied in vitro. We have recently shown on IMR-32, CHP‑134, and LA-N-1 neuroblastoma cells that targeting GD2 with the mAb decr...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2015.3070
更新日期：2015-09-01 00:00:00
abstract：:Nestin is an intermediate filament protein and a stem cell marker expressed in several tumours. There is growing evidence of an association between the expression level of nestin and the pathogenesis of triple-negative breast cancer (TNBC). Nestin is also expressed in newly forming tumour vessels and is a valuable mar...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2017.4223
更新日期：2018-02-01 00:00:00
abstract：:Using two malignant rat glioma cell lines, we tested to what extent peripheral immunization could affect tumor growth in the brain of syngeneic rats. Peripheral subcutaneous (s.c.) immunization was performed with autologous Newcastle-disease-virus (NDV)-infected or non-infected live tumor cells. Thus immunized rats or...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.6.4.759
更新日期：1995-04-01 00:00:00
abstract：:MUC16 (CA125) is a transmembrane mucin that contributes to the progression of epithelial ovarian cancer (EOC). Expression of MUC16 is not detectable in the epithelial surface of normal ovaries. MUC16 expression is, however, common in serous EOC as well as in metastatic and recurrent tumors. Despite these observations,...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2014.2707
更新日期：2015-01-01 00:00:00
abstract：:The p53-induced wig-1 gene encodes an unusual nuclear zinc finger protein with high sequence similarity between human, rat and mouse. Wig-1 belongs to a group of proteins with widely spaced zinc fingers that bind double stranded (ds) RNA. We show here that wig-1 is present in Gallus gallus (chicken), Silurana tropical...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2004-06-01 00:00:00
abstract：:Activation of intestinal human pregnane X receptor (PXR) has recently been proposed as a promising strategy for the chemoprevention of inflammation-induced colon cancer. The present study was aimed at evaluating the effect of rifaximin, a non-absorbable antibiotic, in inhibiting angiogenesis in a model of human colore...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：10.3892/ijo.2016.3550
更新日期：2016-08-01 00:00:00
abstract：:Malignant astrocytomas are highly invasive, vascular neoplasms that comprise the majority of nervous system tumors in humans. A strong association has previously been made between malignancy in human astrocytic tumors and increased expression of certain fibroblast growth factor (FGF) family members. MG-160 is an intri...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2003-05-01 00:00:00
abstract：:Rhabdomyosarcoma (RMS) is a malignant tumour of skeletal muscle origin which includes two major histological subtypes: alveolar rhabdomyosarcoma (ARMS), the more aggressive, and embryonal rhabdomyosarcoma (ERMS). In order to establish whether the higher metastatic potential of ARMS cells may depend on differential exp...
journal_title：International journal of oncology
pub_type： 杂志文章
doi：
更新日期：2005-09-01 00:00:00
