Abstract:
:The presence of cancer stem cells in both solid and hematopoietic malignancies, has been recently linked to their pathogenesis. Sarcomas are rare, and diversely characterized by degrees of mesenchymal differentiation. The aim of the current study was to demonstrate whether the human sarcoma cell lines, osteosarcoma MG63, Ewing's sarcoma HTB166, fibrosarcoma HT1080, possess the stem-like properties which may contribute to the drug-resistance. All cell lines possessed an ability to form spherical, clonal expanding colonies (sarcospheres) in anchorage-independent, serum-starved conditions. Sarcospheres showed the stem-like properties with the ability of self-renewal, and increased expression of the stem cell-related genes such as Nanog, OCT3/4 SOX2 and DNA repair enzyme genes, MLH1 and MSH2. Sarcospheres showed strong resistance to doxorubicin and cisplatin, and caffeine, a DNA repair inhibitor, enhanced the efficacy of those drugs, suggesting that the drug resistance in sarcosphere cells was partly related to the efficient DNA repair ability. These results indicate that human sarcoma cell lines contain stem-like cell populations with strong drug resistance, and DNA repair inhibitor could enhance the efficacy of chemo-drugs against sarcomas.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Fujii H,Honoki K,Tsujiuchi T,Kido A,Yoshitani K,Takakura Ysubject
Has Abstractpub_date
2009-05-01 00:00:00pages
1381-6issue
5eissn
1019-6439issn
1791-2423journal_volume
34pub_type
杂志文章abstract::A rat mammary tumor cell line maintained in low serum (1%) medium exhibited a slower growth rate, altered cell cycle distribution, decreased DNA synthesis, and increased immunoprecipitable p53, when compared with culture in 10% serum. Addition of linoleic acid (LA; 18:2, n-6) to low serum medium partially restored nor...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.1.81
更新日期:1993-07-01 00:00:00
abstract::MCA serum levels were determined in 196 patients with benign breast disease (BD) and in 371 patients with breast cancer. MCA values were abnormal in 30.6% of the patients with BD. In breast cancer patients, significant increases were only found in metastatic disease (89.1%). MCA levels were not correlated with tumor s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::It has previously been suggested that some tumours may elaborate substances which can suppress the functional activity of lymphokine activated killer (LAK) cells. We have investigated the effects of secreted products from the colorectal cancer cell line LoVo on the pattern of expression of T lymphocyte subsets and nat...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.3.4.763
更新日期:1993-10-01 00:00:00
abstract::Transcriptional silencing of the DNA repair protein, O6-methylguanine-DNA methyltransferase (MGMT), occurs only in malignant or transformed cell lines, and such MGMT-deficient cells are hypersensitive to chemotherapeutic alkylating agents such as 1, 3-bis (2-chloroethyl)-1-nitrosourea (BCNU) and temozolomide. Previous...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.18.6.1187
更新日期:2001-06-01 00:00:00
abstract::Induction of the B7 family molecules by 12-O-tetradecanoyl phorbol 13-acetate (TPA) has been reported, however, the mechanism by which TPA up-regulates these molecules remains poorly understood. In this study, the expression of B7-DC, -H1, -H2, and -H3 in response to TPA was markedly induced in K562 cells. TPA also in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::B7-H4 is a transmembrane protein which is a member of the B7 superfamily. It is overexpressed in various types of cancer, including gastric cancer. However, the effects of B7-H4 on the tumorigenicity of gastric cancer and the underlying mechanisms have not yet been fully explored. Thus, the aim of this study was to ex...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4274
更新日期:2018-04-01 00:00:00
abstract::Renal cell carcinoma (RCC) is the most common solid neoplasm of adult kidney, and the major treatment for metastatic RCC (mRCC) is molecular targeted therapy. Sorafenib, as a multi-targeted tyrosine kinase inhibitor (TKI), has significantly improved clinical outcomes of mRCC patients. However, complete or long-term re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3356
更新日期:2016-04-01 00:00:00
abstract::Meningiomas are the most commonly occurring intracranial tumors and account for approximately 15-20% of central nervous system tumors. Patients whose tumors recur after surgery and radiation therapy have limited therapeutic options. It has also been reported recently that radiation triggers DNA repair, cell survival a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.934
更新日期:2011-04-01 00:00:00
abstract::Dexamethasone is a synthetic glucocorticoid frequently used to suppress side-effects of anticancer chemotherapy. In the present study, we showed that dexamethasone treatment leads to concentration-dependent downregulation of cancer-associated marker, carbonic anhydrase IX (CA IX), at the level of promoter activity, mR...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3621
更新日期:2016-10-01 00:00:00
abstract::Reports of the metabolomic characteristics of esophageal cancer are limited. In the present study, we thus conducted metabolome analysis of paired tumor tissues (Ts) and non-tumor esophageal tissues (NTs) using capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS). The Ts and surrounding NTs were surgi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4340
更新日期:2018-06-01 00:00:00
abstract::A promising family of anticancer agents, the camptothecins, is noted for their ability to induce apoptosis specifically in malignant cells. However, a major obstacle for successful cancer treatment by these and other chemotherapeutic agents is the intrinsic or acquired resistance to drug treatment. Resistance to 9NC6,...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::Solid tumors can generate a plethora of neurogenesis-related molecules that enhance their growth and metastasis. Among them, we have identified axonal guidance molecule Semaphorin 7A (SEMA7A) in breast cancer. The goal of this study was to determine the therapeutic effect of suppressing SEMA7A levels in the 4T1 murine...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4144
更新日期:2017-11-01 00:00:00
abstract::Serial analysis of gene expression (SAGE) is a sequence-based tool that enables quantitative study of comprehensive gene expression profiles. In this study, we generated a SAGE library of hepatitis B virus (HBV)-associated small hepatocellular carcinoma (HCC) and compared it with libraries of normal liver and other or...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000321
更新日期:2009-07-01 00:00:00
abstract::Following the publication of this article, the authors noted that there was an error in Fig. 4. The lines representing the two groups were indicated incorrectly in all graphs in Fig. 4. A corrected version of Fig. 4 is presented below. Furthermore, we regret any inconvenience this error may have caused. [the original ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3796
更新日期:2017-02-01 00:00:00
abstract::Combination therapy is a promising and prevalent strategy for osteosarcoma treatment. Curcumin (CUR), as a chemosensitizer, improves the antitumor effect of first‑line chemotherapy drugs. However, due to its poor solubility and instability in physiological conditions, the bioavailability of CUR is limited. In order to...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5067
更新日期:2020-08-01 00:00:00
abstract::Cholangiocarcinoma is the most common biliary duct malignancy and the second most common primary liver cancer, accounting for 10‑20% of hepatic malignancies. With high mortality and poor prognosis, the 5‑year survival rate of cholangiocarcinoma is only 10%. A previous study demonstrated a significant association betwe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5165
更新日期:2020-12-30 00:00:00
abstract::Nitric oxide (NO) shows tumoricidal activity. We had previously reported that NO downregulates the phosphatidylinositol-3-kinase/Akt pathway, but upregulates the MEK/ERK pathway downstream of growth factor signaling. We hypothesized that NO donor and MEK inhibitor in combination syn...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1243
更新日期:2012-03-01 00:00:00
abstract::Cholecystokinin (CCK) and gastrin stimulate growth of pancreatic cancer. Although down-regulation of gastrin inhibits growth of pancreatic cancer, the contribution of endogenous CCK to tumor growth is unknown. The purpose of this study was to evaluate the role of endogenous CCK on autocrine growth of pancreatic cancer...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2010.886
更新日期:2011-03-01 00:00:00
abstract::To clarify the effect of L-histidinol on hyperthermic killing of cells, HeLa and mouse sarcoma-180 (S-180) cells were exposed to heat in vitro in the presence of L-histidinol and the clonogenic surviving fraction of the cells was examined. After pretreating the cells with L-histidinol for 4 h, exposure of the cells to...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1993-11-01 00:00:00
abstract::Although it has been shown that the cross-talk between osteoblasts and tumor cells stimulates proliferation and invasion of prostate carcinoma (PCa) cells, the molecular mechanisms underlying this event are largely unknown. In this study, we demonstrated that the PCa cells, PC3, derived from bone metastasis, undergo c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::Chemotherapy is an important treatment option for gastric cancer (GC); however, chemotherapy usually fails due to drug resistance, particularly multidrug resistance (MDR). In our previous studies, microRNA (miR)‑874 was demonstrated to serve an important role in tumour growth, apoptosis and angiogenesis. In the presen...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4593
更新日期:2018-12-01 00:00:00
abstract::Experimental evidence indicates that prostate apoptosis response-4 (Par-4, also known as PAWR) is a key regulator of cancer cell survival and may be a target for cancer-selective targeted therapeutics. Par-4 was first identified in prostate cancer cells undergoing apoptosis. Both intracellular and extracellular Par-4 ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1983
更新日期:2013-08-01 00:00:00
abstract::Specific RE protein species modulated by autocrine TGF alpha are unknown. HCT116 cells transfected with TGF alpha antisense expression vector were compared with control HCT116 NEO for mitogenesis and modulation of high MW (130, 120 kD) and low MW (116, 110, 105 kD) RE species. Nutrients alone (N) induced maximal mitog...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.6.1025
更新日期:1996-06-01 00:00:00
abstract::Neuroblastoma, originated from neural crest cells, is the most common extracranial solid tumor in childhood. In the present study, we evaluated in vitro the oncolytic effect of live-attenuated poliovirus on human neuroblastoma cell lines, and in vivo its therapeutic efficacy in human neuroblastoma-bearing athymic mice...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Whether elderly patients with metastatic esophageal, gastroesophageal, and gastric cancer do as well with chemotherapy as their younger counterparts was investigated in this pooled analysis. In total, 367 patients from 8 consecutive, first-line trials were included: i) etoposide + cisplatin; ii) 5-fluorourucil + leuco...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000535
更新日期:2010-03-01 00:00:00
abstract::Mutational inactivation of the cyclin-dependent kinase inhibitors (CDKIs) (p16CDKN2a) tumor suppressor gene has been found in a variety of human tumor types. To investigate the involvement of CDKI abnormality in clear cell chondrosarcoma, alterations of CDKIs were examined in clear cell chondrosarcoma tissues using a ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.19.4.749
更新日期:2001-10-01 00:00:00
abstract::Knowledge of intrinsic tumor heterogeneity is vital for understanding of tumor progression mechanisms as well as for providing efficient treatments. In situ proteomic profiling of tumors is a powerful technology with potential to enhance our understanding of tumor biology, but sources of variability due to patient and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::Survivin is a member of the inhibitor of apoptosis family, which is known to inhibit mitochondrial apoptosis. Survivin is highly expressed in cancers and plays an important role in cancer cell survival, and increased survivin expression is an unfavorable prognostic marker in cancer patients. YM155, a novel small-molec...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3077
更新日期:2015-09-01 00:00:00
abstract::Colorectal cancer (CRC) is one of the most common cancers; however, the development of drugs to treat the condition has reached a plateau. Bortezomib (PS-341, Velcade®) is a proteasome inhibitor approved for the treatment of hematological malignancies, including multiple myeloma. A few trials of bortezomib, alone or i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1448
更新日期:2012-07-01 00:00:00
abstract::Conjugated linoleic acid (CLA) refers to a group of naturally occurring positional and geometrical conjugated dienoic isomers of linoleic acid (C18:2), of which the cis-9,trans-11 (c9,t11) and trans-10,cis-12 (t10,c12) isomers predominate. Accumulating evidence has demonstrated that CLA isomers are capable of inhibiti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00