Abstract:
:Combination therapy is a promising and prevalent strategy for osteosarcoma treatment. Curcumin (CUR), as a chemosensitizer, improves the antitumor effect of first‑line chemotherapy drugs. However, due to its poor solubility and instability in physiological conditions, the bioavailability of CUR is limited. In order to improve the physicochemical properties of natural CUR, β‑cyclodextrin was adopted to generate a β‑cyclodextrin curcumin (CD‑CUR) inclusion complex. A thermosensitive hydrogel, poly(D,L‑lactide‑co‑glycolide)-poly(ethylene‑glycol)‑poly(D,L‑lactide‑co‑glycolide), was selected and synthesized to co‑deliver doxorubicin (DOX) and CD‑CUR to tumor sites. The dual‑drug delivery system (gel+DOX+CD‑CUR) was prepared by mixing drugs with hydrogels and had a perfect sol‑gel phase transition temperature (18.3˚C for 20% concentration). Both DOX and CUR were released from hydrogels in a sustained manner in PBS (pH 7.4) medium. The combination therapy based on DOX+CD‑CUR exhibited higher antitumor activity than monotherapies in vitro. Combined CD‑CUR therapy significantly downregulated Bcl‑2 expression and upregulated caspase‑3 expression, suggesting that DOX combined with CD‑CUR treatment has a higher apoptosis‑inducing efficiency. The antitumor efficiency of the gel+DOX+CD‑CUR strategy was evaluated in K‑7 tumor‑bearing mice, and this localized combination therapy demonstrated a higher antitumor efficiency compared with free DOX+CD‑CUR or single‑drug strategies. There were no significant differences in body weight and histological changes of major organs in each group. Therefore, the present combination treatment based on hydrogel may be a feasible approach to co‑deliver DOX and CD‑CUR to osteosarcoma tumor sites in clinical practice.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Yang Z,Liu J,Lu Ydoi
10.3892/ijo.2020.5067subject
Has Abstractpub_date
2020-08-01 00:00:00pages
433-444issue
2eissn
1019-6439issn
1791-2423journal_volume
57pub_type
杂志文章abstract::Cholangiocarcinoma is the most common biliary duct malignancy and the second most common primary liver cancer, accounting for 10‑20% of hepatic malignancies. With high mortality and poor prognosis, the 5‑year survival rate of cholangiocarcinoma is only 10%. A previous study demonstrated a significant association betwe...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5165
更新日期:2020-12-30 00:00:00
abstract::Fatty acid synthase (FASN) is a key enzyme that plays a critical role in numerous metabolic functions by catalyzing the synthesis for long-chain fatty acids. FASN is highly expressed in various human cancers. This preferential expression makes FASN an attractive target for anticance...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1401
更新日期:2012-06-01 00:00:00
abstract::Angiogenesis is increased in various human cancers, including head and neck squamous cell carcinoma (HNSCC), and correlates with tumor progression and metastasis. Vascular endothelial growth factor (VEGF) has been shown to be a key regulator of angiogenesis. We determined whether VEGF antisense oligonucleotide treatme...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::To overcome the poor tumor transduction efficiency of adenovirus serotype 5 (Ad5) observed in several types of cancer, the fiber region of Ad5, apart from its tail, was replaced by adenovirus serotype 35 (Ad35). The chimeric Ad5/F35 adenoviral vector did not exhibit any significant enhancement of transduction efficien...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1812
更新日期:2013-04-01 00:00:00
abstract::Our transcriptome analysis revealed in bladder cancer (BCa) tissues a significant induction of lysosomal-associated multispanning membrane protein 5 (LAPTM5), a lysosomal membrane protein preferentially expressing in immune cells and hematopoietic cells. Transportation of LAPTM5 from Golgi to lysosome could be inhibit...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3788
更新日期:2017-01-01 00:00:00
abstract::We showed in our previous study that cancer upregulated gene (CUG) 2, a novel oncogene, confers resistance to infection of oncolytic vesicular stomatitis virus (VSV) by activating Stat1-mediated signal transduction. Since many studies have reported that autophagy is involved in virus replication, we investigated wheth...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2264
更新日期:2014-04-01 00:00:00
abstract::Cell lines established from the TH-MYCN transgenic murine model of neuroblastoma are a valuable preclinical, immunocompetent, syngeneic model of neuroblastoma, for which knowledge of their p53 pathway status is important. In this study, the Trp53 status and functional response to Nutlin-3 and ionising radiation (IR) w...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4261
更新日期:2018-03-01 00:00:00
abstract::The Raf/MEK/ERK signaling cascade has been extensively studied for its roles in growth and differentiation of a variety of cell types. Confliciting evidence exists regarding the function of classical MAPK signaling with regards to the development of chemotherapeutic drug resistance; some reports describe an pro-surviv...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-06-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is cytotoxic to the majority of cancer cells while sparing most normal cells. However, different prostate carcinoma cell lines respond with different sensitivities to TRAIL, urging us to disclose the mechanisms that determine TRAIL sensitivity in prostate...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::The Tap1 and Tap2 genes encoding for a heterodimeric peptide transporter play a key role in antigen processing and presentation. The TAP complex mediates the transport of peptides generated by the IFN-gamma-inducible proteasome subunits LMP2, 7 and 10 from the cytosol into the endoplasmic reticulum (ER), where they bi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::Human breast cancer is a major cause of global morbidity and mortality in women and it is a process that involves numerous molecular and cellular alterations attributed to environmental substances and agents such as hormones. Bisphosphonates, such as pamidronate, are potent antiresorptive drugs used to the treatment o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2955
更新日期:2015-01-01 00:00:00
abstract::We previously showed that the B cell leukemia cell line NALM-6 had the highest susceptibility among a number of leukemia cell lines to spiruchostatin B (SP-B), a potent histone deacetylase (HDAC) inhibitor. We also showed that SP-B-induced cytotoxicity depended on induction of apopt...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1507
更新日期:2012-09-01 00:00:00
abstract::It has been demonstrated that triptolide inhibits the growth of several types of cancer cells in vitro and prevents tumor growth in vivo by inducing apoptosis and autophagy. Here we showed that Tripchlorolide (T4) significantly suppressed the proliferation of A549 cells in a dose- a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1278
更新日期:2012-04-01 00:00:00
abstract::Observational, clinical and experimental studies have suggested that dietary supplementation with selenium can inhibit the development of colon cancer. Since toxicity and chemopreventive efficacy of selenium compounds depend to a large extent, on the form of selenium the development of efficacious organoselenium compo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.1.29
更新日期:1998-07-01 00:00:00
abstract::It has been reported that XAF1 expression in gastric cancer is negatively correlated with p53. Our purpose was to clarify the regulatory mechanism of p53 on XAF1 expression. The effects of overexpressed wild-type and mutant p53 on XAF1 expression were evaluated. Binding capacity of core XAF1 promoter sequence to the r...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000584
更新日期:2010-04-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is difficult to diagnose early, resulting in only 30% resection rate. HCC is a relatively chemo-resistant tumor, molecular targeted therapy can only benefit approximately 30% patients with liver cancer. Bufalin (Bu) is one of the topoisomerase II inhibitors, many studies have recently fo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3667
更新日期:2016-10-01 00:00:00
abstract::New aromatase inhibitors are an exciting treatment option for postmenopausal women with hormone sensitive breast cancer. They have been shown to reduce tumors in a significant number of patients, and exhibit definite antitumor activity at a relatively low daily dose, and are highly potent, highly selective, and well-t...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.17.5.1037
更新日期:2000-11-01 00:00:00
abstract::Cervical cancer is a potentially preventable disease; however, it is the third most commonly diagnosed cancer and the fourth leading cause of cancer deaths in women worldwide. Cervical cancer is thought to develop through a multistep process involving virus, tumor suppressor genes, proto-oncogenes and immunological fa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2873
更新日期:2015-04-01 00:00:00
abstract::A precise diagnosis of peritoneal dissemination is necessary to determine the appropriate treatment strategy for colorectal cancer. However, small peritoneal dissemination is difficult to diagnose. 5-aminolevulinic acid (5-ALA) is an intermediate substrate of heme metabolism. The administration of 5-ALA to cancer pati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2417
更新日期:2014-07-01 00:00:00
abstract::Myofibroblasts in the stroma of pancreatic cancers promote tumor proliferation, invasion and metastasis by increasing extracellular matrix and secretion of several growth factors. In contrast, the role of myofibroblasts at peritoneally disseminated sites of pancreatic cancer has not yet been determined. This study was...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2391
更新日期:2014-07-01 00:00:00
abstract::The prognosis of non-small cell lung cancer (NSCLC) patients who failed two or more treatment regimens remains very poor. We conducted a phase II study to explore the feasibility of personalized peptide vaccination (PPV), in which peptides are selected and administered based on the pre-existing host immunity before va...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2699
更新日期:2015-01-01 00:00:00
abstract::Erlotinib, an epidermal growth factor receptor tyrosine kinase inhibitor (EGFR‑TKI), is widely applied as a first‑line treatment for non‑small cell lung cancer (NSCLC) and greatly improves the clinical outcomes of patients. However, acquired resistance to EGFR‑TKIs remains a major clinical challenge. Here, we identifi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5086
更新日期:2020-09-01 00:00:00
abstract::Malignant gliomas are treated by combining surgery and radiation with chemotherapy. Cure is rare and utilizing information arising from our improved understanding of brain tumor biology may be of value. Interferon alpha (IFNalpha) treatment as restorative immunotherapy has been utilized in malignant gliomas in the pas...
journal_title:International journal of oncology
pub_type: 临床试验,杂志文章
doi:
更新日期:2004-08-01 00:00:00
abstract::Protein disulfide‑isomerase A3 (PDIA3) is a chaperone protein that modulates folding of newly synthesized glycoproteins and responds to endoplasmic reticulum (ER) stress. Previous studies reported that increased expression of PDIA3 in hepatocellular carcinoma (HCC) is a marker for poor prognosis. However, the mechanis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4710
更新日期:2019-04-01 00:00:00
abstract::We studied the mechanism by which the para and meta positional isomers of nitric oxide-donating aspirin (NO-ASA) inhibit human colon cancer cell growth. These compounds are promising chemopreventive agents and represent a broader class of novel drugs. The two isomers differ drastically in their 24-h IC50s for cell gro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000397
更新日期:2009-10-01 00:00:00
abstract::Liposarcoma (LPS) is one of the most frequently reported type of soft‑tissue sarcoma (STS). Well‑differentiated (WD) LPS and dedifferentiated (DD) LPS are the two most common subtypes. Chemotherapy has been considered to be ineffective in LPS, and novel treatment agents are thus necessary. In this study, we reanalyzed...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4861
更新日期:2019-10-01 00:00:00
abstract::The regions upstream from the human N-myc gene were examined for both transcription and replication. The plasmids containing several N-myc regions linked to the bacterial chloramphenicol acetyltransferase (CAT) gene were transfected to human neuroblastoma IMR32 cells. The results indicated that the region spanning fro...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.3.603
更新日期:1994-09-01 00:00:00
abstract::There is emerging evidence for the prognostic role of various microRNA (miRNA) molecules in colon cancer. The aim of this study was therefore to compare the miRNA profiles in the primary tumor of patients with recurrent and non-recurrent colon cancer. The study population included 110 patients, 51 (46%) with stage I a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1403
更新日期:2012-06-01 00:00:00
abstract::CXC chemokines have a major influence on the angiogenesis, growth and metastatic potential of pancreatic ductal adenocarcinoma. CXCL16 is a unique transmembrane CXC chemokine, which is shed by members of the disintegrins and metalloproteases (ADAMs), in particular by ADAM10 and ADAM17. In our study, we evaluated expre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-08-01 00:00:00
abstract::The Her-2 receptor coded for by the proto-oncogenic erbB-2 gene is a clinically validated target for treatment of a significant genetic subclass of breast cancers, and Her-2 is also overexpressed or mutated in a range of other cancers. In an approach to exploit antisense mediated splicing interference as a means of ma...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00