Abstract:
:Myofibroblasts in the stroma of pancreatic cancers promote tumor proliferation, invasion and metastasis by increasing extracellular matrix and secretion of several growth factors. In contrast, the role of myofibroblasts at peritoneally disseminated sites of pancreatic cancer has not yet been determined. This study was designed to assess the role of myofibroblasts at peritoneally disseminated sites of pancreatic cancer. Three primary cultures of human peritoneal myofibroblasts (hPMFs) were established from disseminated sites of pancreatic cancer and their interactions with the SUIT-2 and CAPAN-1 human pancreatic cancer cell lines were analyzed in vitro. Using a model in BALB/c nu/nu mice, we compared the dissemination ability of intraperitoneally implanted pancreatic cancer cells, with and without hPMFs, and examined the presence of green fluorescent protein (GFP)-labeled hPMFs at peritoneally disseminated sites in mice. hPMFs significantly promoted the migration and invasion of pancreatic cancer cells (P<0.05), while the cancer cells significantly promoted the migration and invasion of hPMFs (P<0.05). In vivo, the number of peritoneally disseminated nodules, more than 3 mm in size, was significantly greater in mice implanted with cancer cells plus hPMFs compared to mice implanted with cancer cells alone, with GFP-labeled hPMFs surviving in the peritoneal cavity of the former. hPMFs promote the peritoneal dissemination of pancreatic cancer. The cancer-stromal cell interaction in the peritoneal cavity may be a new therapeutic target to prevent the dissemination of pancreatic cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Akagawa S,Ohuchida K,Torata N,Hattori M,Eguchi D,Fujiwara K,Kozono S,Cui L,Ikenaga N,Ohtsuka T,Aishima S,Mizumoto K,Oda Y,Tanaka Mdoi
10.3892/ijo.2014.2391subject
Has Abstractpub_date
2014-07-01 00:00:00pages
113-20issue
1eissn
1019-6439issn
1791-2423journal_volume
45pub_type
杂志文章abstract::The aim of the present review was to summarize the current knowledge of the involvement of nestin in breast cancer (BC) pathogenesis. Nestin is a member of the class VI family of intermediate filament proteins, originally identified as a marker of neural stem cells and subsequently demonstrated to be expressed in BC a...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2018.4441
更新日期:2018-08-01 00:00:00
abstract::Serial analysis of gene expression (SAGE) is a sequence-based tool that enables quantitative study of comprehensive gene expression profiles. In this study, we generated a SAGE library of hepatitis B virus (HBV)-associated small hepatocellular carcinoma (HCC) and compared it with libraries of normal liver and other or...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000321
更新日期:2009-07-01 00:00:00
abstract::Macrophages activated by lipopolysaccharide or interferon-gamma have been shown to be cytotoxic to tumor cells by releasing nitric oxide. Here, we report that unstimulated rat alveolar macrophages cultured with certain tumor cells produce nitric oxide and are cytotoxic to these tumor cells. Alveolar macrophages were t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.6.1053
更新日期:1993-06-01 00:00:00
abstract::Erlotinib, an epidermal growth factor receptor tyrosine kinase inhibitor (EGFR‑TKI), is widely applied as a first‑line treatment for non‑small cell lung cancer (NSCLC) and greatly improves the clinical outcomes of patients. However, acquired resistance to EGFR‑TKIs remains a major clinical challenge. Here, we identifi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5086
更新日期:2020-09-01 00:00:00
abstract::Glutathione S-transferase (GST) enzymes catalyse the detoxification of by-products of reactive oxygen species and are thus important in cellular defence mechanisms. The GSTs are polymorphic with allelic variants encoding isoforms with functional differences. GST polymorphism has been associated with susceptibility and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-01-01 00:00:00
abstract::VSELs are a population of rare Oct-4+CXCR4+ CD133+lin-CD45- cells that are deposited during early embryogenesis in developing organs/tissues as a reserve population of pluripotent stem cells for regeneration. We reported recently that they are mobilized into peripheral blood during acute myocardial infarction (AMI) in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000671
更新日期:2010-08-01 00:00:00
abstract::The MUC1 heterodimeric protein is aberrantly overexpressed in diverse human carcinomas and contributes to the malignant phenotype. The MUC1-C transmembrane subunit contains a CQC motif in the cytoplasmic domain that has been implicated in the formation of dimers and in its oncogenic function. The present study demonst...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1308
更新日期:2012-05-01 00:00:00
abstract::Cervical cancer is a potentially preventable disease; however, it is the third most commonly diagnosed cancer and the fourth leading cause of cancer deaths in women worldwide. Cervical cancer is thought to develop through a multistep process involving virus, tumor suppressor genes, proto-oncogenes and immunological fa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2873
更新日期:2015-04-01 00:00:00
abstract::Previous studies from this laboratory have shown that bioflavonoids including luteolin and quercetin possess antiestrogenic activity in the female reproductive tract and appear to elicit this activity by interaction with type II [H-3]estradiol binding sites in the cell nucleus. Studies by a number of laboratories incl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.6.1311
更新日期:1997-12-01 00:00:00
abstract::Cell synchrony was induced in AGF cells by blocking of the cell cycle at GI-S boundary with high concentrations (2 mM) of thymidine for 11 h. Prolonged arrest of cells in GI-S (15 h-20 h) induced progressive and time dependent apoptosis. Early morphological changes in cellular and nuclear morphology (blebbing) were mo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::RhKGF is developed for prevention and treatment of chemotherapy- and radiation-induced epithelial damage in cancer patients. Little information exists on growth modulation of malignant cells by KGF. We have tested the anchorage-independent clonal growth of 35 human tumor and 22 lymphoma and leukemia cell lines in semi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::SAC3 domain containing 1 (SAC3D1) has been reported to be involved in numerous types of cancer. However, the role of SAC3D1 in GC has not yet been elucidated. In the present study, the mRNA expression level of SAC3D1 between GC and normal tissues were assessed with a continuous variable meta‑analysis based on multiple...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5048
更新日期:2020-07-01 00:00:00
abstract::The Tap1 and Tap2 genes encoding for a heterodimeric peptide transporter play a key role in antigen processing and presentation. The TAP complex mediates the transport of peptides generated by the IFN-gamma-inducible proteasome subunits LMP2, 7 and 10 from the cytosol into the endoplasmic reticulum (ER), where they bi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-02-01 00:00:00
abstract::Vanadate has been reported to be involved in the causation of cancer. In this study, we found that both AP-1 and NFkappaB activities were increased after treatment with sodium vanadate in JB6 cells. Maximum induction of AP-1 and NFkappaB appeared at 48 to 72 h. Phosphorylations of Erks and p38 kinases were markedly in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.4.711
更新日期:1998-10-01 00:00:00
abstract::The aim of this study was to examine the level and basic characteristics of cell‑derived microparticles (MPs) in the cyst fluids of odontogenic keratocysts (OKCs). For this purpose, MPs from the cyst fluids (CFMPs) of OKCs were purified by a classic differential centrifugation method and characterized by a transmissio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4361
更新日期:2018-06-01 00:00:00
abstract::We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.3.433
更新日期:2000-09-01 00:00:00
abstract::The objective of the present study was to establish the individual and combined chemopreventive potential of a widely used non-steroidal anti-inflammatory drug, ibuprofen (IBU), encapsulated in solid lipid nanoparticles (SLNs) for the chemoprevention of pancreatic cancer. The IBU SLNs were optimized using various lipi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2879
更新日期:2015-04-01 00:00:00
abstract::Despite attempts to use multiple drug combinations that include gemcitabine (GEM), there is very little evidence that these combination regimens are superior to the single use of this agent. We therefore investigated the suppressive effect of the combination of systemically administered GEM and locally administered in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-07-01 00:00:00
abstract::DNA replication is a vital process in cell division where anomalies can lead to tumorigenesis. Minichromosome maintenance complex component 10 (MCM10) plays a crucial role in this process. However, the role of MCM10 in lung cancer pathogenesis remains to be elucidated. In current study, using the publicly available lu...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4899
更新日期:2019-12-01 00:00:00
abstract::Peroxisome proliferator-activated receptor gamma (PPARγ) agonists have been demonstrated to exert an inhibitory effect on cell growth in several tumor models, including clear cell renal cell carcinoma (CCRCC). PPARγ has therefore been proposed to be a potential therapeutic target. Thus, the PPARγ gene must be expresse...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2010.891
更新日期:2011-03-01 00:00:00
abstract::We recently demonstrated lymphoma development in transgenic mice deficient in retinoic acid receptor alpha (RARalpha). High incidence of lymphoma development in this transgenic mouse model system was similar to lymphoma development in p53 knockout mice. In an effort to understand the molecular basis of lymphomagenesis...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.3.561
更新日期:2000-03-01 00:00:00
abstract::We demonstrated previously that rat ascites hepatoma MM1 cells require both lysophosphatidic acid (LPA) and fibronectin (FN) for phagokinetic motility and transcellular migration and that these events are regulated through the RhoA-ROCK pathway and tyrosine phosphorylation of proteins including focal adhesion kinase (...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::A major limitation of cancer chemotherapy is development of resistance. In this study, we analyzed KB-3-1 cells and adriamycin-selected multidrug resistant sublines KB-A1 and KB-A10 cells for mechanisms of resistance. KB-A10 cells are 10-fold more resistant than KB-A1 cells but have lower P-glycoprotein. Of the known ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.5.1025
更新日期:1997-11-01 00:00:00
abstract::Glioblastoma is the most malignant type of primary brain tumor that has been shown to contain a small population of cancer stem cells. Recent studies have suggested that cancer stem cells cause tumor recurrence based on their resistance to radiotherapy and chemotherapy. Although the highly invasive nature of glioblast...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000764
更新日期:2010-11-01 00:00:00
abstract::Sialyl Le(a) antigen (CA19-9), a member of a family of high molecular weight glycoproteins, was originally described as a gastrointestinal- and pancreatic-specific tumor marker. Recent studies have demonstrated that sialyl Le(a) is a ligand for E-selectin and may play an important role in tumor metastasis. However, ex...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2000-10-01 00:00:00
abstract::The objective of these studies was to examine the murine pharmacokinetics, pharmacodynamics and metabolism of (3-(1H-indol-2-yl)phenyl)(1H-indol-2-yl)methanone (Indole 15), a novel tubulin inhibitor for the treatment of cancer. We developed HPLC and LC/MS/MS assays to quantitate Indole 15 and characterize its metaboli...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1442
更新日期:2012-07-01 00:00:00
abstract::Peripheral blood mononulear cells (PBMC) derived from 10 asymptomatic human T lymphotropic virus type I (HTLV-I) carriers were cultured for a short term (10-14 days) in the absence of exogenous antigens. In 5 carriers, when compared with 5 HTLV-I non-carriers an apparent increase in the proportion of CD8+ DR+ cells wa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.5.2.159
更新日期:1994-08-01 00:00:00
abstract::Extracellular matrix breakdown as well as increased expression in cancer cells and tumor microvascular endothelial cells make matrix metalloproteinase 2 (MMP2) an attractive target for cancer treatment. By taking advantage of MMP2's properties, an MMP2 cleavable melittin/avidin conjugate was designed. Melittin alone i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::A p53 functional analysis system, which can identify the types of abnormality of p53, such as loss of function, dominant negative function, or gain of oncogenic function, is now required. In this study, we examined the functional diversity of several mutations of p53 derived from human head and neck cancer cells. The ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-05-01 00:00:00
abstract::Despite the introduction of 13-cis-retinoic acid (13-cis-RA) into the current chemotherapy, more than half of high-risk neuroblastoma patients have experienced tumor relapses driven by chemoresistant cancer stem cells (CSCs) that can be isolated by their ability to grow as spheres. Although aldehyde dehydrogenase (ALD...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2801
更新日期:2015-03-01 00:00:00