Abstract:
:Previous studies from this laboratory have shown that bioflavonoids including luteolin and quercetin possess antiestrogenic activity in the female reproductive tract and appear to elicit this activity by interaction with type II [H-3]estradiol binding sites in the cell nucleus. Studies by a number of laboratories including our own have shown that type II sites are present in the rodent prostate gland and therefore, we suspected that administration of bioflavonoids such as luteolin may antagonize prostate growth through type LT site binding interactions as well. The studies presented in this report demonstrate that the mouse prostatic tissue does contain nuclear type II sites and that oral administration of luteolin for 14 days results in a significant (p<0.01) reduction in prostatic weight in intact male mice without significant effects on the seminal vesicular, testis or body weights of these animals. These results suggest that luteolin is a prostate specific antagonist under these experimental conditions. In vitro studies with LNCaP and PC-3 human prostate cancer cell lines demonstrated that luteolin treatment resulted in a dose dependent inhibition of prostate cancer cell proliferation which was maximum 4-8 days following treatment. Whole cell binding studies demonstrated that both LNCaP and PC-3 cells contained very high concentrations of type II [H-3]estradiol binding sites (>200,000 sites/cell) relative to levels previously reported for other tissues and cells and luteolin was capable of interacting with these sites in prostate cancer cells. In fact, there was a direct correlation between the type II site occupancy by luteolin and the inhibition of LNCaP or PC-3 prostatic cancer cell proliferation by this bioflavonoid. Flow cytometric analysis revealed that luteolin treatment caused an accumulation of LNCaP cells in G(2)/M (p<0.01) and reduced the fractions of LNCaP cells in S-phase undergoing apoptosis (p<0.01). Similarly, luteolin treatment also arrested PC-3 cells in G(2)/M (p<0.01) and reduced the proportion of cells in G(0)/G(1) (p<0.05). This being the case, it is not surprising that this bioflavonoid also blocked the growth of subcutaneous PC-3 cell xenografts in athymic nude mice. These data demonstrate that naturally occurring type TI site antagonists such as luteolin are capable of inhibiting normal and malignant prostatic cell growth and proliferation in vitro and in viva and may possess prophylactic as well as therapeutic activities against prostatic proliferative diseases including benign prostatic hyperplasia (BPH) and prostate cancer.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Markaverich B,Alejandro Mdoi
10.3892/ijo.11.6.1311subject
Has Abstractpub_date
1997-12-01 00:00:00pages
1311-9issue
6eissn
1019-6439issn
1791-2423journal_volume
11pub_type
杂志文章abstract::Melanoma, the most aggressive human skin tumor, has a very short survival time, and there are currently no effective treatments. Alterations in cell metabolism, such as enhanced aerobic glycolysis, have been identified as hallmarks of cancer cells. In the present study, bioinformatics studies using online databases re...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.4964
更新日期:2020-03-01 00:00:00
abstract::By setting the cut-off line at the level as used in a negative control study without primary antibody in the same sample, the Ki-67 labeling rate was calculated by the Ki67-DNA dual fluorescence analysis. The mean Ki-67 labeling rate of 28 advanced gastric cancer was 45.1% (13.9-76.3%). The Ki-67 labeling rates were s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.1.3.265
更新日期:1992-08-01 00:00:00
abstract::Cytokines of the TGF beta family are thought to be involved in cellular growth control and are therefore likely candidates to regulate homeostasis of the prostate. We have analyzed immunohistochemically the expression of TGF-beta 3 in normal prostate (NP), benign prostate hyperplasia (BPH) and prostate cancer (PCa). I...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.6.1185
更新日期:1997-12-01 00:00:00
abstract::Oncogenic, moderately immunogenic MK16/1/IIIABC (MK16) cells were previously established by co-transfection of HPV 16 E6/E7 and activated H-ras oncogene DNA into C57BL/6 kidney cells. Subcutaneous transplantation of the MK16 cells produced progressively growing neoplasms which metastasized spontaneously to lungs. In t...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.23.1.243
更新日期:2003-07-01 00:00:00
abstract::Transforming growth factor beta1 (TGF-beta1) has been shown to exhibit anti-proliferative activity for mammary gland epithelial cells and for human breast cancer cells. Insulin-like growth factor I (IGF-I), in contrast, is a well-characterized mitogenic and anti-apoptotic factor involved in mammary gland physiology. I...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.2.277
更新日期:2000-02-01 00:00:00
abstract::We have previously reported that Insulin-like growth factor (IGF-I) is a cellular growth factor of human transformed T-lymphocytes. To evaluate the role of IGF-I for nontransformed T-cells we investigated synthesis of IGF-I and the influence of an IGF-I antiserum, UBK487, during proliferation process. IGF-I is synthes...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2.6.1003
更新日期:1993-06-01 00:00:00
abstract::TGF-beta1 stimulates proliferation of WI38 human embryo fibroblasts but inhibits that of their SV40-transformed counterparts, VA13 cells. Protein expression levels of cyclins A, D1, E and that of cdk2 and cdk4 were not affected by TGF-beta1 in either of these cells. However, TGF-beta1-treatment increases cdk2 kinase a...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.5.905
更新日期:1999-05-01 00:00:00
abstract::It has been reported that suppression of selenium‑binding protein 1 (SBP1) occurs in many human malignancies which is considered to play an important role in cancer development and progression. Despite its importance in cancer, the function and factors that regulate its expression are not known. Using cell proliferati...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1850
更新日期:2013-05-01 00:00:00
abstract::Prostate cancer, the third most common cancer in men worldwide, varies substantially according to geographic region and race/ethnicity. Obesity and associated endocrine variation are foremost among the risk factors that may underlie these regional and ethnic differences. The association between obesity and prostate ca...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:
更新日期:2006-03-01 00:00:00
abstract::Induction of the B7 family molecules by 12-O-tetradecanoyl phorbol 13-acetate (TPA) has been reported, however, the mechanism by which TPA up-regulates these molecules remains poorly understood. In this study, the expression of B7-DC, -H1, -H2, and -H3 in response to TPA was markedly induced in K562 cells. TPA also in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-12-01 00:00:00
abstract::MCA serum levels were determined in 196 patients with benign breast disease (BD) and in 371 patients with breast cancer. MCA values were abnormal in 30.6% of the patients with BD. In breast cancer patients, significant increases were only found in metastatic disease (89.1%). MCA levels were not correlated with tumor s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1993-02-01 00:00:00
abstract::Solid tumors can generate a plethora of neurogenesis-related molecules that enhance their growth and metastasis. Among them, we have identified axonal guidance molecule Semaphorin 7A (SEMA7A) in breast cancer. The goal of this study was to determine the therapeutic effect of suppressing SEMA7A levels in the 4T1 murine...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4144
更新日期:2017-11-01 00:00:00
abstract::The MyD118/Gadd45/CR6 gene family (also termed Gadd45beta/alpha/gamma) has been identified as genes which are rapidly induced by genotoxic agents, during terminal differentiation, as well as by apoptotic cytokines. In recent years, evidence has emerged that the proteins encoded by these genes play pivotal roles in neg...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-04-01 00:00:00
abstract::The aim of the present study was to assess whether (-)-epigallocatechin 3-gallate (EGCG) via epigenetic modifications, regulates Raf kinase inhibitor protein (RKIP) expression and invasive metastatic activity in AsPC-1 pancreatic adenocarcinoma cells. Basal levels of RKIP were examined in various human pancreatic canc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1686
更新日期:2013-01-01 00:00:00
abstract::Anisi stellati fructus (ASF), commonly known as star anise, has long been used as a traditional Chinese medicine to treat inflammation, nervousness, insomnia and pain. In recent studies, it has been demonstrated that ASF possesses anti-bacterial, anti-fungal and anti-oxidant activities, as well as exhibits inhibitory ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2606
更新日期:2014-11-01 00:00:00
abstract::Normal human diploid cells have a limited life span and undergo replicative senescence after various limited population doublings. Cells must pass the senescence barrier to become immortal. The exact mechanisms of immortalization are not clear, although inactivation of the RB pathway, and/or the p53 pathway and activa...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::Epidemiologic studies on the protective role of omega-3 fatty acids (n:3) on breast cancer prevention remain inconclusive but studies in preclinical models provide more positive outcome. However, the mechanisms accounting for the protective effect of n:3 are not defined. In the present study, conducted in the N-methyl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1133
更新日期:2011-11-01 00:00:00
abstract::Five retinoblastoma tumours from patients treated in the King Khaled Eye Specialist Hospital in Riyadh were analysed for mutations in the RB1 gene using single strand conformation polymorphism analysis and mutations were found in all of them. In one tumour the mutation in exon 11 was homozygous demonstrating the role ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.3.687
更新日期:1995-03-01 00:00:00
abstract::Observational, clinical and experimental studies have suggested that dietary supplementation with selenium can inhibit the development of colon cancer. Since toxicity and chemopreventive efficacy of selenium compounds depend to a large extent, on the form of selenium the development of efficacious organoselenium compo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.13.1.29
更新日期:1998-07-01 00:00:00
abstract::Hsp90α is a molecular chaperone protein involved in the structural maturation of oncogenic signaling proteins. Hsp90 was recently identified as an anticancer target; various studies are ongoing to find ways for managing cancer through Hsp90α. However, this approach is limited by reported side-effects. Hypoxia is a hal...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1450
更新日期:2012-07-01 00:00:00
abstract::Upregulation of nuclear factor-κB (NF-κB) in colorectal carcinoma (CRC) accelerates tumor growth, whereas, irinotecan (CPT-11)-induced NF-κB activation reduces chemosensitivity and weakens the anti-colorectal cancer function itself, while proteasome inhibitors can inhibit NF-κB and improve the effect of chemotherapy. ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2513
更新日期:2014-09-01 00:00:00
abstract::The insulin-like growth factors (IGFs) are a family of mitogenic proteins involved in the regulation of cell growth and differentiation. The presence and role of the IGF system in oral mucosal epithelium is not clear but could influence our understanding of the pathogenesis of oral cancer. We characterised the express...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-10-01 00:00:00
abstract::The tumour suppressor gene hypermethylated in cancer 1 (HIC1) is a transcriptional repressor, which functionally cooperates with p53. Loss of HIC1 function is associated with the development of various tumor entities. The aim of this study was to elucidate the relevance of CpG islan...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1367
更新日期:2012-05-01 00:00:00
abstract::MicroRNA‑301a (miRNA/miR‑301a) and nuclear factor (NF)‑κB signaling play important roles in tumor invasion, migration and progression. However, the role of miRNA‑301a‑3p in human gastric cancer (GC), and specifically in the activation of NF‑κB signaling, remains unclear. The aim of the present study was to investigate...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2020.5072
更新日期:2020-08-01 00:00:00
abstract::Malignant pleural mesothelioma (MPM) is a rare and aggressive form of cancer commonly associated with asbestos exposure that stems from the thoracic mesothelium with high mortality rate. Currently, treatment options for MPM are limited, and new molecular targets for treatments are urgently needed. Using quantitative r...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3566
更新日期:2016-08-01 00:00:00
abstract::The prognosis for patients with head and neck tumors (HNSCC) is poor, due among other things to the high-risk factor for locoregional recurrence and/or second primary tumors. Extensive studies on chemoprevention of oral pre-cancers to stop carcinogenesis and to prevent recurrence and/or second primary tumors have fail...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1139
更新日期:2011-11-01 00:00:00
abstract::Wilms' tumor is one of the most common malignant tumors of the abdomen in children. However, there is currently no recognized specific biomarker for the clinical diagnosis and prognosis of this tumor. Lipid metabolism is involved in membrane synthesis and oxidation in tumor cells. This process plays an important role ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4948
更新日期:2020-02-01 00:00:00
abstract::Human pancreatic ductal adenocarcinomas overexpress the epidermal growth factor (EGF) receptor. Betacellulin is a mitogenic polypeptide that binds and activates this receptor. To determine whether betacellulin has a role in human pancreatic cancer, we studied its expression in cultured human pancreatic cancer cell lin...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.7.4.825
更新日期:1995-10-01 00:00:00
abstract::Dose is a critical determinant of the efficacy of chemotherapy. The dose response curve for virtually all anticancer agents is steep in both in vitro and in vivo experimental studies. However the application of the steep dose-response curve in the clinic has been troublesome for many years because of dose limiting bon...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-04-01 00:00:00
abstract::Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is promising for cancer treatment owing to its selective cytotoxicity against malignant cells. However, some cancer cell types, including malignant melanoma cells, are resistant to TRAIL-induced apoptosis. Therefore, dr...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1483
更新日期:2012-08-01 00:00:00