Prophylactic, therapeutic and anti-metastatic effects of BMDC and DC lines in mice carrying HPV 16-associated tumours.


:Oncogenic, moderately immunogenic MK16/1/IIIABC (MK16) cells were previously established by co-transfection of HPV 16 E6/E7 and activated H-ras oncogene DNA into C57BL/6 kidney cells. Subcutaneous transplantation of the MK16 cells produced progressively growing neoplasms which metastasized spontaneously to lungs. In this communication we report that prophylactic administration of bone marrow-derived dendritic cells (BMDC) as well as dendritic cell (DC) lines DC2.4 and JAWS II at the site of subsequent MK16 tumour transplants inhibited tumour growth and reduced the number of lung metastases. Similarly, in therapeutic experiments, administration of BMDC and DC lines at the site of the growing MK16 tumours or at the site of MK16 tumour residua after surgery inhibited tumour growth. Both BMDC-based vaccines and vaccines based on DC lines had also an antimetastatic effect. These results indicate that the DC line-based vaccines, which represent a standard, well-characterized and more homogeneous material, technically easier to prepare than the fresh BMDC-based vaccines, can be utilized for therapy of surgical minimal residual disease in HPV 16-associated neoplasms and are prospective for relevant clinical trials.


Int J Oncol


Mendoza L,Bubeník J,Símová J,Jandlová T,Vonka V,Mikysková R





Has Abstract


2003-07-01 00:00:00












  • Stronger growth-inhibitory effect of interferon (IFN)-beta compared to IFN-alpha is mediated by IFN signaling pathway in hepatocellular carcinoma cells.

    abstract::Interferon (IFN) is a promising drug for prevention and treatment of hepatocellular carcinoma (HCC) in combination with chemotherapeutic agents. We previously reported that the spectra of antiproliferative activity and synergistic effect of IFN-beta when combined with anticancer drugs are more potent than those of IFN...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Damdinsuren B,Nagano H,Wada H,Kondo M,Ota H,Nakamura M,Noda T,Natsag J,Yamamoto H,Doki Y,Umeshita K,Dono K,Nakamori S,Sakon M,Monden M

    更新日期:2007-01-01 00:00:00

  • ERK-modulated intrinsic signaling and G(2)/M phase arrest contribute to the induction of apoptotic death by allyl isothiocyanate in MDA-MB-468 human breast adenocarcinoma cells.

    abstract::Allyl isothiocyanate (AITC), a member of the isothiocyanate (ITC) family found in a constituent of cruciferous vegetables, possesses anticancer activity and induces apoptosis in various types of human cancer cell lines. However, no available information showed antitumor effects in human breast adenocarcinoma cells. Th...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Tsai SC,Huang WW,Huang WC,Lu CC,Chiang JH,Peng SF,Chung JG,Lin YH,Hsu YM,Amagaya S,Yang JS

    更新日期:2012-12-01 00:00:00

  • HIF1-positive and HIF1-negative glioblastoma cells compete in vitro but cooperate in tumor growth in vivo.

    abstract::Glioblastoma multiforme (GBM) is characterized by extensive angiogenesis that is mostly orchestrated by the hypoxia inducible factor HIF-1. Deregulation of HIF-1 is believed to contribute to cancer initiation and progression. However, instances have been described in which loss of HIF-1 leads to more aggressive tumors...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Fiorenzo P,Mongiardi MP,Dimitri D,Cozzolino M,Ferri A,Montano N,Trevisi G,Maira G,Battistini L,Falchetti ML,Levi A,Pallini R

    更新日期:2010-04-01 00:00:00

  • RNA interference-mediated targeting of urokinase plasminogen activator receptor and matrix metalloproteinase-9 gene expression in the IOMM-lee malignant meningioma cell line inhibits tumor growth, tumor cell invasion and angiogenesis.

    abstract::Meningiomas are the most commonly occurring tumors of the central nervous system including the brain and spinal cord. Malignant meningiomas are highly aggressive and frequently recur after surgical resection of the tumor. Our previous studies have reported that urokinase plasminogen activator receptor (uPAR) and matri...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Tummalapalli P,Gondi CS,Dinh DH,Gujrati M,Rao JS

    更新日期:2007-07-01 00:00:00

  • Nuclear-DNA content and p53 immunostaining in oral squamous-cell carcinoma - an analysis of a consecutive 10-year material.

    abstract::In a retrospective analysis of 91 consecutive oral carcinomas we reviewed all patient records and weighed clinicopathological parameters against the results of image DNA cytometry and p53 immunhistochemical staining of the initial diagnostic biopsies. Eighty-seven percent of the biopsies were either aneuploid or nondi...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Hogmo A,Munckwikland E,Kuylenstierna R,Lindholm J,Auer G

    更新日期:1994-10-01 00:00:00

  • Involvement of Bcl-2 family members, phosphatidylinositol 3'-kinase/AKT and mitochondrial p53 in curcumin (diferulolylmethane)-induced apoptosis in prostate cancer.

    abstract::Curcumin (diferulolylmethane), an active ingredient derived from the rhizome of the plant Curcuma longa, has anticancer activity in vitro and in vivo. Although curcumin possesses chemopreventive properties against several types of cancer, the molecular mechanisms by which it inhibits cell growth and induces apoptosis ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Shankar S,Srivastava RK

    更新日期:2007-04-01 00:00:00

  • Long‑term exposure to gefitinib induces acquired resistance through DNA methylation changes in the EGFR‑mutant PC9 lung cancer cell line.

    abstract::This study was designed to identify epigenetically regulated genes and to clarify the contribution of epige-netic alteration to acquired resistance to epidermal growth factor receptor‑tyrosine kinase inhibitors (EGFR‑TKIs). We established a gefitinib‑resistant lung cancer cell line, PC9, which was originally gefitinib...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Terai H,Soejima K,Yasuda H,Sato T,Naoki K,Ikemura S,Arai D,Ohgino K,Ishioka K,Hamamoto J,Kanai Y,Betsuyaku T

    更新日期:2015-01-01 00:00:00

  • Identification of proteins associated to multi-drug resistance in LoVo human colon cancer cells.

    abstract::Multi-drug resistance (MDR) limits the effectiveness of chemotherapy. P-glycoprotein encoded by the MDR1 gene, is known to be implicated in MDR phenotype, but other factors could be determinant in MDR. The aim of this study was to investigate new molecular factors potentially associated with the MDR phenotype using a ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Visentin M,Simula MP,Sartor F,Petrucco A,De Re V,Toffoli G

    更新日期:2009-05-01 00:00:00

  • Soluble low-molecular-mass tumor-associated proteins promote the suppression of mammary tumors by cyclophosphamide.

    abstract::This study examined whether the soluble tumor-associated-antigens (TAA), of 66 kDa and 51 kDa, could promote suppression by anticancer drugs of chemically-induced mammary tumorigenesis. Dimethylbenzanthracene (DMBA, 10 mg/rat, twice) was used to induce mammary tumors. Then, for nine more weeks, the preparation of TAA ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ben-Hur H,Kossoy G,Zusman I

    更新日期:2001-08-01 00:00:00

  • HIF-1alpha attenuates tumor growth in spite of augmented vascularization in an A549 adenocarcinoma mouse model.

    abstract::The hypoxia-inducible transcription factor, HIF-1, adapts cells to low oxygen tension. In addition to the activation of angiogenesis by induction of VEGF, HIF-1 may trigger hypoxia-induced growth arrest and apoptosis. The aim of this study was to analyze the overall effect of HIF-1 on tumor growth in a mouse model, em...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Savai R,Schermuly RT,Voswinckel R,Renigunta A,Reichmann B,Eul B,Grimminger F,Seeger W,Rose F,Hänze J

    更新日期:2005-08-01 00:00:00

  • Isoform switch of CD44 induces different chemotactic and tumorigenic ability in gallbladder cancer.

    abstract::Gallbladder cancer (GBC) is one of the most unfavorable prognostic tumor, and immediate growth and distant metastasis are important factors associated with the poor prognosis of patients with this disease. Standard and variant isoforms of CD44 are associated with tumor growth, metastasis, and epithelial-mesenchymal tr...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Miwa T,Nagata T,Kojima H,Sekine S,Okumura T

    更新日期:2017-09-01 00:00:00

  • Arsenic trioxide induces apoptosis through a reactive oxygen species-dependent pathway and loss of mitochondrial membrane potential in HeLa cells.

    abstract::Arsenic trioxide (As2O3) can induce clinical remission in patients with acute promyelocytic leukemia (APL) through induction of apoptosis. To investigate the potential therapeutic usage of As2O3 in cervical cancer and its possible mechanisms, human cervical cancer cell line HeLa was employed. The cells underwent apopt...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Woo SH,Park IC,Park MJ,Lee HC,Lee SJ,Chun YJ,Lee SH,Hong SI,Rhee CH

    更新日期:2002-07-01 00:00:00

  • Tumor hypoxia: Impact on gene amplification in glioblastoma.

    abstract::Gene amplification is frequently found in human glioblastoma but the mechanisms driving amplifications remain to be elucidated. Hypoxia as hallmark of glioblastoma is known to be involved in the induction of fragile sites that are central to gene amplification. We analyzed the potential of hypoxia (pO2 0%) and mini hy...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Fischer U,Radermacher J,Mayer J,Mehraein Y,Meese E

    更新日期:2008-09-01 00:00:00

  • Reversal of drug resistance using hammerhead ribozymes against multidrug resistance-associated protein and multidrug resistance 1 gene.

    abstract::We examined the effects of suppressing multidrug resistance-associated protein (MRP) and multidrug resistance 1 (MDR1) gene expression in HCT-8DDP human colon cancer cell lines, which showed both cisplatin and multidrug resistance. Hammerhead ribozymes, designed to cleave MRP mRNA (anti-MRP Rz) and MDR1 mRNA (anti-MDR...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Nagata J,Kijima H,Hatanaka H,Asai S,Miyachi H,Abe Y,Yamazaki H,Nakamura M,Watanabe N,Mine T,Kondo T,Scanlon KJ,Ueyama Y

    更新日期:2002-11-01 00:00:00

  • Prognostic-significance of p53 mutations in small non-cleaved cell lymphomas.

    abstract::We have assessed the prognostic significance of mutations in the p53 tumor suppressor gene in patients with small non-cleaved cell lymphomas. In this retrospective pilot study we have, been able to evaluate the response to therapy of 21 previously untreated patients. Seven of these patients (33%) had tumors which cont...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Gutierrez M,Bhatia K,Diez B,Muriel F,Epelman S,Deandreas M,Huppi K,Magrath I

    更新日期:1994-03-01 00:00:00

  • Characterization of cells resistant to the potent histone deacetylase inhibitor spiruchostatin B (SP-B) and effect of overexpressed p21waf1/cip1 on the SP-B resistance or susceptibility of human leukemia cells.

    abstract::We previously showed that the B cell leukemia cell line NALM-6 had the highest susceptibility among a number of leukemia cell lines to spiruchostatin B (SP-B), a potent histone deacetylase (HDAC) inhibitor. We also showed that SP-B-induced cytotoxicity depended on induction of apopt...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kanno S,Maeda N,Tomizawa A,Yomogida S,Katoh T,Ishikawa M

    更新日期:2012-09-01 00:00:00

  • GAS64, the first amplified and putative non-translated gene.

    abstract::Gene amplification occurs frequently in human glioblastomas. We report the amplification and expression analysis of glioma amplified sequence 64 (GAS64) which was recently identified by microdissection mediated cDNA capture from the amplicon at 12q13-15. Herein we analyzed primary tumor tissues for amplification frequ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Fischer U,Schütz N,Hemmer D,Meese E

    更新日期:2002-01-01 00:00:00

  • Genetic alterations in metastatic renal cell carcinoma detected by comparative genomic hybridization: correlation with clinical and histological data.

    abstract::In order to optimize the management of patients with renal cell carcinoma (RCC) it is important to define the genetic risk for metastatic disease. In this study we performed comparative genomic hybridization (CGH) on metastatic tumors aiming at the identification of genetic alterations associated with metastatic disea...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Junker K,Moravek P,Podhola M,Weirich G,Hindermann W,Janitzky V,Schubert J

    更新日期:2000-11-01 00:00:00

  • CA IX is upregulated in CoCl2-induced hypoxia and associated with cell invasive potential and a poor prognosis of breast cancer.

    abstract::Hypoxia, a common phenomenon during the development of malignant solid tumors including breast cancer, serves to propagate a cascade of molecular pathways triggered by hypoxia-inducible factor-1α (HIF-1α). Carbonic anhydrase IX (CA IX), one of the target genes of HIF-1α, has been reported to be involved in progression...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Chu CY,Jin YT,Zhang W,Yu J,Yang HP,Wang HY,Zhang ZJ,Liu XP,Zou Q

    更新日期:2016-01-01 00:00:00

  • Activation of RB tumor suppressor protein and growth suppression of small cell lung carcinoma cells by reintroduction of p16INK4A gene.

    abstract::The p16INK4A tumor suppressor gene is frequently inactivated in non-small cell lung carcinoma (NSCLC) and is less frequently inactivated in small cell lung carcinoma (SCLC) by mutation, deletion or DNA methylation. There are several reports that the reintroduction of the p16INK4A gene into p16(-) NSCLC cells results i...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Sumitomo K,Shimizu E,Shinohara A,Yokota J,Sone S

    更新日期:1999-06-01 00:00:00

  • The novel synthesized 2-(3-(methylamino)phenyl)-6-(pyrrolidin-1-yl)quinolin-4-one (Smh-3) compound induces G2/M phase arrest and mitochondrial-dependent apoptotic cell death through inhibition of CDK1 and AKT activity in HL-60 human leukemia cells.

    abstract::2-Phenyl-4-quinolone series compounds have exhibited growth inhibitory influence on several human cancer cell lines. In this study, we investigated the effects of 2-(3-(methylamino)phenyl)-6-(pyrrolidin-1-yl)quinolin-4-one (Smh-3) on viability, cell cycle and apoptotic cell death which occurred in different leukemia c...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Huang SM,Yang JS,Tsai SC,Chen MH,Hsu MH,Lin HY,Chou LC,Chinag JH,Lee KH,Huang LJ,Kuo SC

    更新日期:2011-05-01 00:00:00

  • Gene amplification at chromosome 1pter-p33 including the genes PAX7 and ENO1 in squamous cell lung carcinoma.

    abstract::Gene amplification is a frequent event in lung cancer, specifically in squamous cell lung carcinoma. Recently, we reported amplifications on chromosomal bands 3q26.1-q26.3 with the genes BCHE and SLC2A2 amplified in 40% of squamous cell lung carcinomas. Here, we identified an amplified domain within chromosomal bands ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Rácz A,Brass N,Höfer M,Sybrecht GW,Remberger K,Meese EU

    更新日期:2000-07-01 00:00:00

  • Licochalcone A induces apoptosis in malignant pleural mesothelioma through downregulation of Sp1 and subsequent activation of mitochondria-related apoptotic pathway.

    abstract::Licochalcone A (LCA) is a natural product derived from the roots of Glycyrrhiza inflata exhibiting a wide range of bioactivities such as antitumor, anti-oxidant and anti-bacterial effects. Malignant pleural mesothelioma (MPM) is an extremely aggressive type of cancer with a poor prognosis because of its rapid progress...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Kim KH,Yoon G,Cho JJ,Cho JH,Cho YS,Chae JI,Shim JH

    更新日期:2015-03-01 00:00:00

  • Expression of the SRY-related HMG box protein SOX2 in human gastric carcinoma.

    abstract::SOX2, a SRY-related HMG box protein, is thought to be an important transcription factor during organogenesis, including the stomach although the expression and function are unclear. We investigated SOX2 protein expression to clarify its roles in differentiation and carcinogenesis of the stomach. Using polyclonal SOX2 ...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Li XL,Eishi Y,Bai YQ,Sakai H,Akiyama Y,Tani M,Takizawa T,Koike M,Yuasa Y

    更新日期:2004-02-01 00:00:00

  • Downregulation of β3 integrin by miR-30a-5p modulates cell adhesion and invasion by interrupting Erk/Ets‑1 network in triple-negative breast cancer.

    abstract::Integrins are adhesion receptors involved in bidirectional signaling and are crucial for various cellular responses during normal homeostasis and pathological conditions, such as cancer progression and metastasis. In the present study, we demonstrated that blockage of β3 integrin-mediated cell- extracellular matrix in...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Li W,Liu C,Zhao C,Zhai L,Lv S

    更新日期:2016-03-01 00:00:00

  • Hypericin: a promising fluorescence marker for differentiating between glioblastoma and neurons in vitro.

    abstract::The naturally occurring photosensitizer, hypericin, with its high quantum yield of singlet oxygen photogeneration was studied for its ability to differentiate between glioblastoma cells and fetal rat neurons using fluorescence microscopy. Eight human glioma cell lines and twelve primary human glioma cell cultures were...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Ritz R,Müller M,Weller M,Dietz K,Kuci S,Roser F,Tatagiba M

    更新日期:2005-12-01 00:00:00

  • Sentinel node localisation: A new prospective in the treatment of nodal melanoma metastases.

    abstract::Sentinel node (SN) mapping and biopsy is a procedure that accurately stages the regional lymph node (LN) basin. Defined patterns of lymphatic drainage allow intraoperative determination of the first (sentinel) lymph node in the regional basin, and the absence of metastatic disease in the SN accurately reflects the abs...

    journal_title:International journal of oncology

    pub_type: 杂志文章,评审


    authors: Gennari R,Stoldt HS,Bartolomei M,Zurrida S,Testori A,Mazzarol G,Paganelli G,Veronesi U

    更新日期:1999-07-01 00:00:00

  • Regulatory T cells actively infiltrate metastatic brain tumors.

    abstract::Regulatory T cells (CD4+CD25+FoxP3+, Treg) have been shown to play a major role in suppression of the immune response to malignant gliomas. In this study, we investigated the kinetics of Treg infiltration in metastatic brain tumor models, including melanoma, breast and colon cancers. Our data indicate that both CD4+ a...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Sugihara AQ,Rolle CE,Lesniak MS

    更新日期:2009-06-01 00:00:00

  • PPARγ is functionally expressed in clear cell renal cell carcinoma.

    abstract::Peroxisome proliferator-activated receptor gamma (PPARγ) agonists have been demonstrated to exert an inhibitory effect on cell growth in several tumor models, including clear cell renal cell carcinoma (CCRCC). PPARγ has therefore been proposed to be a potential therapeutic target. Thus, the PPARγ gene must be expresse...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Collet N,Théoleyre S,Rageul J,Mottier S,Jouan F,Rioux-Leclercq N,Fergelot P,Patard JJ,Masson D,Denis MG

    更新日期:2011-03-01 00:00:00

  • Histone deacetylase 3 promotes pancreatic cancer cell proliferation, invasion and increases drug-resistance through histone modification of P27, P53 and Bax.

    abstract::Pancreatic cancer is one of the most aggressive solid malignancies with a dismal survival rate. Recent studies have shown that high expression levels of histone deacetylase 3 (HDAC3) correlate with malignant phenotype. However, the expression patterns and biological role of HDAC3 in pancreatic cancer remain unclear. I...

    journal_title:International journal of oncology

    pub_type: 杂志文章


    authors: Jiao F,Hu H,Yuan C,Jin Z,Guo Z,Wang L,Wang L

    更新日期:2014-10-01 00:00:00