Abstract:
:One of the early events of apoptosis is proteolysis. The enzyme(s) involved in this event appear to be the serine protease(s), inasmuch as the reversible or irreversible inhibitors of serine proteases prevent DNA degradation and abrogate other signs of apoptosis in several cell systems (Gorczyca et al, Int J Oncol 1: 639-648, 1992). In the present studies, using multiparameter flow cytometry, we attempted to characterize the rate of disappearance of the nuclear and nucleolar proteins [Proliferating Cells Nuclear Antigen (PCNA), Ki-67, p120 and another nucleolar protein] detected by mononuclear antibodies, during apoptosis of HL-60 cells. Apoptosis was induced by the topoisomerase I inhibitor camptothecin and by the intercalating and nonintercalating topoisomerase inhibitors m-AMSA and teniposide, respectively, and was specific to cells in S phase. The loss of the protein reactive with Ki-67 antibody was the most rapid: two hours after administration of the drugs few S phase cells that expressed this protein remained in the cultures. Nearly all cells which were more advanced in apoptosis, and, due to extensive DNA degradation, had an already diminished DNA content, were negative with respect to expression of Ki-67. The nucleolar proteins, especially the one detected by the antibody distributed by Chemicon, appeared to be more stable compared to Ki-67. The most stable was PCNA: expression of this protein was high even after 6 h of treatment with each of the drugs, even in cells which had reduced DNA content. The data indicate that the proteolytic step is not entirely random and may involve different proteases having different substrate preferences or specific targeting of individual proteins for the degradation. Because spontaneous apoptosis appears to be common in tumors, the phenomenon of rapid degradation of some of the proliferation-associated antigens during apoptosis should be taken into account in the analysis of expression of these proteins in tumors (e.g. for estimation of the tumor growth fraction).
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Gorczyca W,Ardelt B,Darzynkiewicz Zdoi
10.3892/ijo.3.4.627subject
Has Abstractpub_date
1993-10-01 00:00:00pages
627-34issue
4eissn
1019-6439issn
1791-2423journal_volume
3pub_type
杂志文章abstract::The aminoacyl-tRNA synthetase interacting multi-functional protein 1 (AIMP1) participates in a variety of cellular processes, including translation, cell proliferation, inflammation and wound healing. Previously, we showed that the N-terminal peptide of AIMP1 (6-46 aa) induced ERK phosphorylation. Liver fibrosis is ch...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3303
更新日期:2016-02-01 00:00:00
abstract::The potential of arsenic trioxide (As2O3) as a novel therapy against ovarian cancer has been progressively recognized. Its prospective usefulness for treatment of this malignancy either alone or in combination with other chemotherapeutic agents has been increasingly explored. In this study, we attempted to enhance the...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.986
更新日期:2011-06-01 00:00:00
abstract::We assessed Stanniocalcin-1 (STC-1) mRNA expression in human normal tissues, various types of human cancer cell lines, and cancer tissues obtained during surgery. Using a reverse transcriptase-polymerase chain reaction (RT-PCR) assay developed to detect STC-1 mRNA, the transcripts were detected in 20 out of 21 cancer ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.4.799
更新日期:2000-04-01 00:00:00
abstract::The aim of the present review was to summarize the current knowledge of the involvement of nestin in breast cancer (BC) pathogenesis. Nestin is a member of the class VI family of intermediate filament proteins, originally identified as a marker of neural stem cells and subsequently demonstrated to be expressed in BC a...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2018.4441
更新日期:2018-08-01 00:00:00
abstract::The presence of arachidonate 12-lipoxygenase (12-LOX) potentiates prostate cancer (PCa) progression and therefore may be a good therapeutic target and/or a potential diagnostic predictor for PCa. In this study, we examined the expression of 12-LOX in PCa stem cells (PCa SCs) to test if it can serve as a unique marker ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.901
更新日期:2011-04-01 00:00:00
abstract::Telomerase activation in differentiated cells is associated with cell immortalization. Detection of telomerase expression in cancer provides a potential biomarker to be evaluated in the diagnosis and prognosis of cancer patients. Analysis of telomerase activity in biopsy specimens may serve as a useful marker for the ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.2.381
更新日期:2000-08-01 00:00:00
abstract::Vascular endothelial growth factor (VEGF), a secreted endothelial-specific growth factor, and microvessel density was examined in 204 primary non-small cell lung carcinomas. The expression of VEGF was evaluated by immunohistochemical staining using an anti-VEGF antibody. Positive staining was obtained in 125 out of 20...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.5.1059
更新日期:1995-05-01 00:00:00
abstract::Five retinoblastoma tumours from patients treated in the King Khaled Eye Specialist Hospital in Riyadh were analysed for mutations in the RB1 gene using single strand conformation polymorphism analysis and mutations were found in all of them. In one tumour the mutation in exon 11 was homozygous demonstrating the role ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.3.687
更新日期:1995-03-01 00:00:00
abstract::TP53 gene, encoding tumor protein P53, is frequently inactivated in various types of human cancer, including colorectal, lung, and gastric cancer. Cancer cells with TP53 mutation acquire malignant potentials, such as selective growth advantage, genomic instability, resistance to apoptosis, and promotion of angiogenesi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-07-01 00:00:00
abstract::We established a new lymphoma cell line, designated CTB-1, from pericardial effusion of a patient with diffuse large B-cell lymphoma. This cell line showing vigorous growth ability has undergone 260 passages over a period of 34 months in suspension culture, and is heterotransplantable to nude mice. The cultured cells ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.6.1103
更新日期:1997-06-01 00:00:00
abstract::The overall goal of the present study was to evaluate the chemotherapeutic and cancer‑protective properties of D‑erythro‑sphingosine (sphingosine) and C2‑ceramide using a human breast epithelial cell (HBEC) culture system, which represents multiple‑stages of breast carcinogenesis. The HBEC model includes Type I HBECs ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4641
更新日期:2019-02-01 00:00:00
abstract::Benzyl isothiocyanate (BITC) is known for its pharmacological properties against malignant neoplasm, including bladder cancer (BC). The current study investigated microRNAs (miRNA or miR) expression profiles with an emphasis on the role of miR‑99a‑5p in BITC‑treated BC cells. A quantitative polymerase chain reaction (...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2019.4763
更新日期:2019-06-01 00:00:00
abstract::TRAIL (tumor necrosis factor-related apoptosis-inducing ligand) preferentially induces apoptosis of cancer cells without toxicity in normal cells. TRAIL plays an important role in host immune surveillance against tumor metastasis. Cathepsin B (CB) is a mediator of apoptosis whose activity is regulated by its inhibitor...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-01-01 00:00:00
abstract::The active metabolite of vitamin D, 1alpha,25-dihydroxyvitamin D3, can induce differentiation in breast cancer cells; however, it is hypercalcemic in vivo. Therefore, development of non-calcemic analogs of vitamin D has received considerable attention. Recently, we synthesized an analog of vitamin D [1alpha(OH)D5] tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.1.65
更新日期:2000-01-01 00:00:00
abstract::Induction chemotherapy provides an excellent model for evaluation of potential predictive factors. We studied expression of SBR grade, estrogen (ER) and progesterone (PR) receptors, HER2, Ki67 and P53 on core biopsies before and after chemotherapy in a series of 115 patients, who received anthracycline-based induction...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::The tumor suppressor gene product p53 has been detected in a high percentage of esophageal squamous cell carcinoma. To evaluate the role of this protein in carcinogenesis, we examined the p53 overexpression both in esophageal dysplasia and in esophageal squamous cell carcinoma in the same patients. Using anti-p53 anti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.5.1061
更新日期:1994-05-01 00:00:00
abstract::High levels of mitotic progression-associated PLK1 and stress-associated HSF1 have been observed in various human cancers. In the present study, we investigated the effects of PLK1 and HSF1 knockdown on the proliferation of oral cancer cells using small interfering RNA. In human oral squamous cell carcinoma (SCC) tiss...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000564
更新日期:2010-04-01 00:00:00
abstract::Pulmonary adenocarcinoma (PAC) is the leading type of lung cancer and is highly resistant to conventional cancer therapy. A better understanding of the regulatory mechanisms which control the growth of this deadly malignancy are urgently needed to develop more effective cancer intervention strategies. Recent studies h...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-09-01 00:00:00
abstract::Regulation of cytoplasmic p21CIP/WAF1 (p21) is of great clinical significance in molecular oncology due to its identification as an antiapoptotic factor, a poor survival predictor as well as a drug-resistance inducer. A retrospective study of the immunohistochemical (IHC) profiles of 128 human primary breast cancers s...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-11-01 00:00:00
abstract::Antisense oligonucleotides have been investigated as anticancer agents administered alone or in combination with conventional chemotherapeutics. In the present study, we demonstrated synergistic effects between anti-MDM2 antisense oligonucleotides and the clinically used anticancer agent irinotecan, using nude mouse m...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-04-01 00:00:00
abstract::Shc protein is known to be related to cell proliferation and carcinogenesis. However, the involvement of Shc in hepatocellular carcinoma (HCC) remains unknown. In the present study, we report that p46 Shc is probably expressed in the nuclei of hepatocytes and/or cancer cells during the development of HCC in Long-Evans...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-10-01 00:00:00
abstract::Recent studies have reported that chemically synthesized small activating RNA (saRNA) targeting the promoter regions of a gene can activate its expression in different cell lines. This technique can be a powerful therapeutic method for diseases caused by complete inactivation or reduced expression of specific genes. E...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3643
更新日期:2016-10-01 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) is a low-grade lymphoid malignancy incurable with conventional modalities of chemotherapy. We aimed to examine the proapoptotic effects of a novel proteasome inhibitor, N,N'-di-(m-methylphenyi)-3,6- dimethyl-1,4-dihydro-1,2,4,5-tetrazine-1,4-dicarboamide (ZGDH...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1467
更新日期:2012-08-01 00:00:00
abstract::Human chorionic gonadotropin (hCG) inhibits the growth of human breast epithelial cells MCF-7. Differential display was utilized for analyzing RNA of hCG-treated MCF-7 cells in order to determine whether this effect was mediated by specific gene activation or repression. Three cDNA clones, 19, 29 and 44, were differen...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract::Abnormal accumulation and activation of the recepteur d'origine nantais (RON) has been implicated in epithelial tumor carcinogenesis. In the present study, we examined the effect of epigallocatechin-3-gallate (EGCG), the major green tea catechin, on the induction of RON and tumor growth in human gastric cancer. EGCG i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2013.1775
更新日期:2013-03-01 00:00:00
abstract::The hypoxia-inducible transcription factor, HIF-1, adapts cells to low oxygen tension. In addition to the activation of angiogenesis by induction of VEGF, HIF-1 may trigger hypoxia-induced growth arrest and apoptosis. The aim of this study was to analyze the overall effect of HIF-1 on tumor growth in a mouse model, em...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-08-01 00:00:00
abstract::Gene amplification is a frequent event in lung cancer, specifically in squamous cell lung carcinoma. Recently, we reported amplifications on chromosomal bands 3q26.1-q26.3 with the genes BCHE and SLC2A2 amplified in 40% of squamous cell lung carcinomas. Here, we identified an amplified domain within chromosomal bands ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.1.67
更新日期:2000-07-01 00:00:00
abstract::Gicerin, an immunoglobulin superfamily protein, is expressed abundantly in the embryonic tissues and plays an important role in development through its cell adhesive activity. Interestingly, re-expression of gicerin is found in a variety of tumors. In the present study, the possible role of gicerin in the progression ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-12-01 00:00:00
abstract::Acquirement of multi-drug resistance by tumor cells represents a major obstacle in the management of prostate cancer. Such resistance was demonstrated in the androgen-independent DU-145 cells in response to paclitaxel and the mechanisms by which these cell develops resistance was not understood. The objective of this ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::Solid tumors can generate a plethora of neurogenesis-related molecules that enhance their growth and metastasis. Among them, we have identified axonal guidance molecule Semaphorin 7A (SEMA7A) in breast cancer. The goal of this study was to determine the therapeutic effect of suppressing SEMA7A levels in the 4T1 murine...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.4144
更新日期:2017-11-01 00:00:00