Abstract:
:Gene amplification is a frequent event in lung cancer, specifically in squamous cell lung carcinoma. Recently, we reported amplifications on chromosomal bands 3q26.1-q26.3 with the genes BCHE and SLC2A2 amplified in 40% of squamous cell lung carcinomas. Here, we identified an amplified domain within chromosomal bands 1pter-p33 in squamous cell lung carcinoma using reverse chromosome painting. A panel of nine genes which have previously been assigned to region 1pter-p33 was tested for amplification using comparative PCR. The ENO1 gene that encodes enolase and the PAX7 gene that encodes a transcription factor were most frequently amplified. Specifically, the gene ENO1 was amplified in six and the gene PAX7 in five out of 37 cases which included both biopsies and paraffin-embedded tissues of squamous cell lung carcinomas. In total, we identified amplifications of at least one gene at bands 1pter-p33 in 10 out of 37 tumors (27%). Together, our data indicate that a novel and frequent amplification unit is present in squamous cell lung carcinoma with the center of the amplified domain in the vicinity of the genes PAX7 and ENO1.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Rácz A,Brass N,Höfer M,Sybrecht GW,Remberger K,Meese EUdoi
10.3892/ijo.17.1.67keywords:
subject
Has Abstractpub_date
2000-07-01 00:00:00pages
67-73issue
1eissn
1019-6439issn
1791-2423journal_volume
17pub_type
杂志文章abstract::Retroviral infection of hTERT, the catalytic component of telomerase, into BJ fibroblasts (population doubling 28) resulted in reconstitution of telomerase activity, telomere maintenance, and extension of in vitro lifespan. The hTERT-infected cells also exhibited increased growth rate and colony forming efficiency rel...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::Extracellular matrix breakdown as well as increased expression in cancer cells and tumor microvascular endothelial cells make matrix metalloproteinase 2 (MMP2) an attractive target for cancer treatment. By taking advantage of MMP2's properties, an MMP2 cleavable melittin/avidin conjugate was designed. Melittin alone i...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-01-01 00:00:00
abstract::The active metabolite of vitamin D, 1alpha,25-dihydroxyvitamin D3, can induce differentiation in breast cancer cells; however, it is hypercalcemic in vivo. Therefore, development of non-calcemic analogs of vitamin D has received considerable attention. Recently, we synthesized an analog of vitamin D [1alpha(OH)D5] tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.16.1.65
更新日期:2000-01-01 00:00:00
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journal_title:International journal of oncology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.3892/ijo.16.5.1021
更新日期:2000-05-01 00:00:00
abstract::Cytogenetic analyses of peripheral blood cells of breast cancer patients unveiled in one case a grossly enlarged G-band in one copy of chromosome 16 (16qh+), which scored also strongly positive in C-banding. Fluorescence in situ hybridization with both a total chromosome 16 library and with probe pHuR195 detecting loc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.2.347
更新日期:1994-02-01 00:00:00
abstract::The α6 integrin subunit (α6) has been implicated in cancer cell migration and in the progression of several malignancies, but its role in tumor angiogenesis is unclear. In mice, anti-α6 blocking antibodies reduce tumor angiogenesis, whereas Tie1-dependent α6 gene deletion enhances neovessel formation in melanoma and l...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2631
更新日期:2014-11-01 00:00:00
abstract::Surfactant proteins play important roles in lung surfactant function and innate immunity. The DNA methylation state of 11 CpG sites of surfactant protein (SP)-A1, -B, -C, and -D was determined using universal bead arrays. A total of 90 cancerous and non-cancerous tissues from 23 patients with adenocarcinoma and 22 wit...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-07-01 00:00:00
abstract::Lumican is a member of a small leucine-rich proteoglycan family and is highly expressed in several types of cancer cells and/or stromal tissue. Lumican expression in the cytoplasm in advanced colorectal cancer correlates with poor patient prognosis. The expression of lumican in stromal tissues is associated with a hig...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-12-01 00:00:00
abstract::The cytosine analogue 5'-azacytidine (5'-aza) induces DNA hypomethylation by inhibiting DNA methyltransferase. In clinical trials, 5'-aza is widely used in epigenetic anticancer treatments. Accumulated evidence shows that cyclooxygenase-2 (COX-2) is overexpressed in various cancers, indicating that it may play a criti...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3110
更新日期:2015-10-01 00:00:00
abstract::Sonic hedgehog (SHH), Indian hedgehog (IHH) and Desert hedgehog (DHH) are Hedgehog family ligands, which play key roles in embryogenesis and carcinogenesis, especially in pancreatic cancer and gastric cancer. Drosophila Costal-2 (Cos2) is a Hedgehog signaling regulator, interacting with Smoothened (Smo), Cubitus inter...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::Estrogens act through interactions with estrogen receptors (ERs) to play diverse roles in various pathophysiological conditions. A number of synthetic selective estrogen receptor modulators (SERMs), such as tamoxifen and raloxifene, have been developed and used to treat ER-related diseases, including breast cancer and...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3002
更新日期:2015-07-01 00:00:00
abstract::Cell synchrony was induced in AGF cells by blocking of the cell cycle at GI-S boundary with high concentrations (2 mM) of thymidine for 11 h. Prolonged arrest of cells in GI-S (15 h-20 h) induced progressive and time dependent apoptosis. Early morphological changes in cellular and nuclear morphology (blebbing) were mo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1993-09-01 00:00:00
abstract::Pemetrexed (PEM) is a novel, multitargeted, antifolate, antineoplastic agent for the treatment of non-small cell lung cancer and malignant pleural mesothelioma. Additional effects of nitric oxide (NO) donors on the chemosensitivity of cancers have been reported. However, the effects of an NO donor on PEM-induced cytot...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1461
更新日期:2012-07-01 00:00:00
abstract::Neuroblastoma is the second most common extracranial malignant solid tumor that occurs in childhood, and metastasis is one of the major causes of death in neuroblastoma patients. The epithelial-mesenchymal transition (EMT) is an important mechanism for both the initiation of tumor invasion and subsequent metastasis. T...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3954
更新日期:2017-05-01 00:00:00
abstract::We previously reported that podophyllotoxin acetate (PA) radiosensitizes NCI-H460 cells. Here, we confirmed that PA treatment also induces cell death among two other non-small cell lung cancer (NSCLC) cell lines: NCI-H1299 and A549 cells (IC50 values = 7.6 and 16.1 nM, respectively). Our experiments further showed tha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.3123
更新日期:2015-10-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::DNA double-strand breaks (DSBs) are potentially mutagenic/carcinogenic lesions. Induction of DSBs triggers phosphorylation of histone H2AX on Ser-139. Phosphorylated H2AX (gammaH2AX) can be detected immunocytochemically, and the intensity of gammaH2AX immunofluorescence (IF), reflecting the number of gammaH2AX-IF foci...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-06-01 00:00:00
abstract::The enzyme poly(ADP-ribose)polymerase (PARP) has been implicated in several cellular processes by the demonstration of ADP-ribosylation of proteins which play a role in cellular repair of DNA damage, response to ionizing radiation and apoptosis. In addition, ADP-ribosylation has been shown to play a role in cell cycle...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.8.4.803
更新日期:1996-04-01 00:00:00
abstract::With a view to identifying markers reflecting not the evidence of, but the potential for, neoplastic progression of the breast we have compared the normal invasive mammary epithelial cell growth seen at puberty with invasive and non-invasive carcinogenesis using the mouse as a model. We have analyzed cell proliferatio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Orlistat is an orally administered anti-obesity drug that has shown significant antitumor activity in a variety of tumor cells. To identify the proteins involved in its antitumor activity, we employed a proteomic approach to reveal protein expression changes in the human ovarian can...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2012.1465
更新日期:2012-08-01 00:00:00
abstract::Human Frizzled-7 (FZD7) and human FzE3, showing 98.8% nucleotide identity, encode almost identical WNT receptors with nine amino-acid substitutions. FzE3 is claimed to be expressed specifically in esophageal cancer. We determined the structure of the FZD7 gene and the FZD7 cDNA expressed in esophageal cancer. The FZD7...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2001-07-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-05-01 00:00:00
abstract::We have reported that ONO-4007, a novel synthetic lipid A derivative with low toxic activities, produced large amount of tumor necrosis factor (TNF)-alpha selectively in the tumor tissues and brought about complete cures in about 60% of rats bearing TNF-alpha sensitive KDH-8 cells, which also secreted a large amount o...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00
abstract::We have developed a new type of drug delivery system (DDS) comprising a complex of porous hydroxyapatite (HAP) with the anticancer drug doxorubicin hydrochloride (DOX) and the glutathione inhibitor buthionine sulfoximine (BSO) (DOX and BSO-HAP complex). We then studied the antitumour effect of DOX and BSO-HAP combined...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.5.961
更新日期:1997-05-01 00:00:00
abstract::Salinomycin (SAL) is a polyether ionophore antibiotic that has recently been shown to regulate a variety of cellular responses in various human cancer cells. However, the effects of SAL on metastatic capacity of HT1080 human fibrosarcoma cells have not been elucidated. We investigated the effect of SAL on migration an...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3448
更新日期:2016-06-01 00:00:00
abstract::Alternative polyadenylation and microRNA regulation are both mechanisms of post-transcriptional regulation of gene expression. Alternative polyadenylation often results in mRNA isoforms with the same coding sequence but different lengths of 3' UTRs, while microRNAs regulate gene expression by binding to specific mRNA ...
journal_title:International journal of oncology
pub_type: 杂志文章,评审
doi:10.3892/ijo.2013.2047
更新日期:2013-10-01 00:00:00
abstract::Although it is well established that ras genes contribute to tumourigenesis either through the accumulation of mutations or by aberrant expression in a wide range of human cancers, little is known regarding their involvement in human nasal polyps (NPs). In the present study, the occurrence of mutations in codons 11 an...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2007-11-01 00:00:00
abstract::Telomerase activity and its subunits (hTERC, hTERT mRNA) were evaluated in ovarian tumours in relation to the expression of p53, c-Myc and estrogen receptor (ER). Furthermore, relations between telomerase activity, hTERC and hTERT with known clinicopathologic prognostic factors and survival in patients with malignant ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.23.5.1451
更新日期:2003-11-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-12-01 00:00:00
abstract::Multiple drug resistance is reported to be a major obstacle in treatment of osteosarcoma (OS). Research has demonstrated that small subsets of cells called cancer stem cells (CSCs) are responsible for multiple drug resistance. CSCs are potential targets for reversing chemoresistance. In the present study, we compared ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3950
更新日期:2017-05-01 00:00:00