Abstract:
:Retroviral infection of hTERT, the catalytic component of telomerase, into BJ fibroblasts (population doubling 28) resulted in reconstitution of telomerase activity, telomere maintenance, and extension of in vitro lifespan. The hTERT-infected cells also exhibited increased growth rate and colony forming efficiency relative to controls, while remaining contact-inhibited and maintaining a p53-mediated damage response following gamma-irradiation. All single cell-derived BJ-hTERT clones grew faster than the hTERT mass cultures and maintained telomeres; however, neither telomerase activity levels nor mean telomere length correlated with the growth rate. Introduction of hTERT rescued aged BJ fibroblasts from senescence via a telomere-dependent mechanism and provided renewed proliferative potential. Collectively, our data indicate that both early and late in the cellular lifespan of human cells, ectopic expression of telomerase using a retroviral system provides a growth advantage while maintaining normal cellular characteristics.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Forsythe HL,Elmore LW,Jensen KO,Landon MR,Holt SEkeywords:
subject
Has Abstractpub_date
2002-06-01 00:00:00pages
1137-43issue
6eissn
1019-6439issn
1791-2423journal_volume
20pub_type
杂志文章abstract::Thirty-one patients with metastatic breast cancer not responding or progressing after initial response to adriamycin + cyclophosphamide (AC) treatment entered a phase 11 study with oral lonidamine in association to AC. Objective clinical responses were observed in 10 patients (32%) and consisted of 1 complete + 9 part...
journal_title:International journal of oncology
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doi:10.3892/ijo.3.2.213
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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journal_title:International journal of oncology
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pub_type: 杂志文章
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journal_title:International journal of oncology
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更新日期:2002-11-01 00:00:00
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journal_title:International journal of oncology
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更新日期:2008-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2010-10-01 00:00:00
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journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2015.2885
更新日期:2015-04-01 00:00:00
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pub_type: 杂志文章
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journal_title:International journal of oncology
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更新日期:2004-12-01 00:00:00
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journal_title:International journal of oncology
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