Abstract:
:The presence of arachidonate 12-lipoxygenase (12-LOX) potentiates prostate cancer (PCa) progression and therefore may be a good therapeutic target and/or a potential diagnostic predictor for PCa. In this study, we examined the expression of 12-LOX in PCa stem cells (PCa SCs) to test if it can serve as a unique marker and therapeutic target for PCa SCs. To this end, we isolated the cancer stem cell-like side population (SP) cells from the human PCa cell line DU-145 by a flow cytometry-based SP technique. The isolated DU-145 SP cells comprised a small population of the DU-145 cells. The SP cells had an up-regulation of ATP-binding cassette sub-family G member 2 (ABCG2) which enables these cells to efflux vital dyes and chemotherapeutic drugs. Furthermore, we detected a strong up-regulation of 12-LOX in these DU-145 SP cells compared to the parental DU-145 cells by RT-PCR and Western blot approaches. We also detected 12-LOX overexpression in PCa SCs in human PCa tissue samples by paraffin-section based immunofluorescent 4-channel confocal microscopy. However, no 12-LOX was detected in normal prostate epithelial SCs in normal prostate tissue samples. These multiple lines of evidence support the possibility that 12-LOX may serve as a unique marker and therapeutic target for PCa SCs.
journal_name
Int J Oncoljournal_title
International journal of oncologyauthors
Yin B,Yang Y,Zhao Z,Zeng Y,Mooney SM,Li M,Xu X,Song Y,Wu B,Yang Zdoi
10.3892/ijo.2011.901subject
Has Abstractpub_date
2011-04-01 00:00:00pages
1041-6issue
4eissn
1019-6439issn
1791-2423journal_volume
38pub_type
杂志文章abstract::Metastatic colorectal cancer is the second most common cause of cancer mortality. The liver is a common site of metastasis and only a minority of patients with liver metastases are candidates for potentially curative surgical resection. The treatment of patients with unresectable liver tumors is a major clinical probl...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2005-07-01 00:00:00
abstract::Several lines of direct evidence show that gliomas express high levels of vascular endothelial growth factor (VEGF). VEGF can promote the growth of gliomas through angiogenesis. It is believed that gliomas originate in the brain tumor stem cells (BTSCs). However, the direct effect of VEGF on the biological behavior of...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2331
更新日期:2014-05-01 00:00:00
abstract::Using two malignant rat glioma cell lines, we tested to what extent peripheral immunization could affect tumor growth in the brain of syngeneic rats. Peripheral subcutaneous (s.c.) immunization was performed with autologous Newcastle-disease-virus (NDV)-infected or non-infected live tumor cells. Thus immunized rats or...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.6.4.759
更新日期:1995-04-01 00:00:00
abstract::Telomerase activity and its subunits (hTERC, hTERT mRNA) were evaluated in ovarian tumours in relation to the expression of p53, c-Myc and estrogen receptor (ER). Furthermore, relations between telomerase activity, hTERC and hTERT with known clinicopathologic prognostic factors and survival in patients with malignant ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.23.5.1451
更新日期:2003-11-01 00:00:00
abstract::Although it has been shown that the cross-talk between osteoblasts and tumor cells stimulates proliferation and invasion of prostate carcinoma (PCa) cells, the molecular mechanisms underlying this event are largely unknown. In this study, we demonstrated that the PCa cells, PC3, derived from bone metastasis, undergo c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2006-04-01 00:00:00
abstract::It has been reported that XAF1 expression in gastric cancer is negatively correlated with p53. Our purpose was to clarify the regulatory mechanism of p53 on XAF1 expression. The effects of overexpressed wild-type and mutant p53 on XAF1 expression were evaluated. Binding capacity of core XAF1 promoter sequence to the r...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000584
更新日期:2010-04-01 00:00:00
abstract::Thymidylate synthase (TS) is known to have polymorphisms in the 5' and 3' untranslated region (UTR). These polymorphisms have been reported to be associated with high TS expression and chemoresistance to 5-FU. The aim of this study was to examine the prognostic roles of the 5'-UTR and 3'-UTR TS polymorphisms in esopha...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Lack of life-prolonging therapies has provided much of the impetus for seeking complementary and alternative management/treatment options by prostate cancer (CaP) patients. Among these, the use of dietary supplements and botanical products has been showing a sustained increase in recent years, owing in part to some en...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-12-01 00:00:00
abstract::Adrenomedullin (AM) is a multifunctional 52-amino acid peptide. AM has several effects and acts as a growth factor in several types of cancer cells. Our previous study revealed that an AM antagonist (AMA) suppressed the growth of pancreatic tumors in mice, although its mechanism was not elucidated. In this study, we c...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo_00000622
更新日期:2010-06-01 00:00:00
abstract::The MUC1 heterodimeric protein is aberrantly overexpressed in diverse human carcinomas and contributes to the malignant phenotype. The MUC1-C transmembrane subunit contains a CQC motif in the cytoplasmic domain that has been implicated in the formation of dimers and in its oncogenic function. The present study demonst...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1308
更新日期:2012-05-01 00:00:00
abstract::Steroid receptor analysis is the only widely accepted prognostic/predictive marker in breast cancer (BC) treatment. In the present study we evaluated the prognostic role of ER/PgR with p53 and Bcl2, in a series of 277 BC (153 pN1/2, 122 pNO, 2 pNx) with a long-term follow-up (67 months for DFS, 75 months for OS). Our ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.15.6.1137
更新日期:1999-12-01 00:00:00
abstract::Hepatitis B X-interacting protein (HBXIP) is a novel oncoprotein. We have previously reported that HBXIP promotes the proliferation and migration of breast cancer cells. S-phase kinase-associated protein 2 (Skp2) is another oncoprotein which is important for migration. In this study, we investigated whether Skp2 is in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2411
更新日期:2014-07-01 00:00:00
abstract::The aim was to compare the efficacy of ondansetron and a combination of ondansetron plus dexamethasone in the prophylaxis of cisplatin-induced delayed emesis over three consecutive courses of chemotherapy. Cancer patients scheduled to receive for the first time cisplatin (>50 mg/m(2)) in combination with other cytotox...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.10.2.395
更新日期:1997-02-01 00:00:00
abstract::Cancer stem cells (CSC) exhibit therapy resistance and drive self-renewal of the tumour, making cancer stem cells an important target for therapy. The PI3K signalling pathway has been the focus of considerable research effort, including in glioblastoma (GBM), a cancer that is notoriously resistant to conventional ther...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3510
更新日期:2016-07-01 00:00:00
abstract::Chlorophyllin (CHL) is the sodium-copper salts of chlorophyll derivatives. In this study, the CHL was derived from a traditional Chinese medicine, the Bombyx mori excreta. The CHL, at concentrations 25-400 microg/ml, reduced the proliferation of HL-60, K-562, S-180, and MCF-7 cells by 8.2-95.7% after 72 h of incubatio...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2003-09-01 00:00:00
abstract::Many genes that are frequently mutated in human cancer are known to be involved in the control of normal cellular proliferation. One of the genes involved in DNA mismatch repair is hMLH1, defective mutations of which are found in some familial and various sporadic cancers. Although the DNA mismatch repair activity of ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.12.3.609
更新日期:1998-03-01 00:00:00
abstract::Protein kinase C (PKC) designates a family of kinases that regulate many essential functions including cell growth and differentiation. The tight regulation of PKC activity is crucial for maintaining normal cellular proliferation and excessive activity leads to abnormal or uncontrolled cell growth. Recent reports indi...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.14.2.327
更新日期:1999-02-01 00:00:00
abstract::Placenta-specific protein 1 (PLAC1) expression is co-opted in numerous human cancers. As a consequence of PLAC1 expression, tumor cells exhibit enhanced proliferation and invasiveness. This characteristic is associated with increased aggressiveness and worse patient outcomes. Recently, the presence of the tumor suppre...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3931
更新日期:2017-05-01 00:00:00
abstract::An overview of the current mechanisms identified as associated with the expression of multidrug resistance is provided. The clinical relevance of multidrug resistance associated with the overexpression of the three major drug resistance-associated proteins namely, P-glycoprotein, MRP and/or LRP, is discussed. Alternat...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::The Her-2 receptor coded for by the proto-oncogenic erbB-2 gene is a clinically validated target for treatment of a significant genetic subclass of breast cancers, and Her-2 is also overexpressed or mutated in a range of other cancers. In an approach to exploit antisense mediated splicing interference as a means of ma...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2010-01-01 00:00:00
abstract::Multiple drug resistance is reported to be a major obstacle in treatment of osteosarcoma (OS). Research has demonstrated that small subsets of cells called cancer stem cells (CSCs) are responsible for multiple drug resistance. CSCs are potential targets for reversing chemoresistance. In the present study, we compared ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2017.3950
更新日期:2017-05-01 00:00:00
abstract::The aberrant expression of p53 and RB proteins was investigated by immunohistochemistry in 73 patients with esophageal SCC, who underwent esophageal resection. Fresh tumor tissues from 21 patients were also tested for p53 mutations in exons 4 through 9 by direct sequencing. Positive nuclear staining with p53 antibody ...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.11.5.1109
更新日期:1997-11-01 00:00:00
abstract::5-Fluorouracil (5-FU), a fluoropyrimidine analogue, is one of the most commonly used anticancer drugs for the treatment of gastrointestinal malignancies. Some studies reported that the cytotoxicity of fluoropyrimidines is mediated, in large part, by inhibition of the thymidylate synthase (TS), an essential DNA synthet...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2004-01-01 00:00:00
abstract::Investigators have shown that green tea may decrease the risk of cancer. It is widely accepted that the main active component of green tea is EGCG (epigallocatechin-3-gallate). In our previous study, we examined the effect of green tea on breast cancer growth and endothelial cells both in in vitro assays and in animal...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:
更新日期:2002-09-01 00:00:00
abstract::Cytogenetic analyses of peripheral blood cells of breast cancer patients unveiled in one case a grossly enlarged G-band in one copy of chromosome 16 (16qh+), which scored also strongly positive in C-banding. Fluorescence in situ hybridization with both a total chromosome 16 library and with probe pHuR195 detecting loc...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.4.2.347
更新日期:1994-02-01 00:00:00
abstract::Polo-like kinase 1 (PLK1), a serine/threonine kinase and an oncogene, is crucial in regulating cell cycle progression. PLK1 also has been demonstrated as a potential target of osteosarcoma (OS) by using short hairpin RNA libraries in lentiviral vectors for screening of protein kinase. In preclinical studies, GSK461364...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2016.3352
更新日期:2016-03-01 00:00:00
abstract::The prognosis for patients with head and neck tumors (HNSCC) is poor, due among other things to the high-risk factor for locoregional recurrence and/or second primary tumors. Extensive studies on chemoprevention of oral pre-cancers to stop carcinogenesis and to prevent recurrence and/or second primary tumors have fail...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2011.1139
更新日期:2011-11-01 00:00:00
abstract::Colorectal cancer (CRC) treatment primarily relies on chemotherapy along with surgery, radiotherapy and, more recently, targeted therapy at the late stages. However, chemotherapeutic drugs have high cytotoxicity, and the similarity between the effects of these drugs on cancerous and healthy cells limits their wider us...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2018.4536
更新日期:2018-11-01 00:00:00
abstract::Nasopharyngeal carcinoma (NPC) is a major cause of cancer deaths. Concurrent administration of radiation and chemotherapy is the treatment of choice for advanced NPC. Previously, we showed that apogossypolone (ApoG2) induced apoptosis by blocking the binding of Bcl-2 to Bax, arresting the cell cycle in the S phase, in...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.2014.2497
更新日期:2014-09-01 00:00:00
abstract::We describe and discuss the findings by fluorescent in situ hybridization (FISH) for detection of non-random chromosomal gains, in a group of unusual fibrous lesions in children. Nuclear disaggregation was used to prepare slides from eight cases which were hybridized using alpha-satellite enumeration probes for chromo...
journal_title:International journal of oncology
pub_type: 杂志文章
doi:10.3892/ijo.17.3.433
更新日期:2000-09-01 00:00:00