Low incidence of genetic alterations of the p16CDKN2a in clear cell chondrosarcoma.

Abstract:

:Mutational inactivation of the cyclin-dependent kinase inhibitors (CDKIs) (p16CDKN2a) tumor suppressor gene has been found in a variety of human tumor types. To investigate the involvement of CDKI abnormality in clear cell chondrosarcoma, alterations of CDKIs were examined in clear cell chondrosarcoma tissues using a quantitative DNA/PCR, PCR-SSCP. Two of 38 specimens (5.2%) we analyzed showed abnormally low levels of p16CDKN2a amplification, suggesting that the allelic deletion of the gene might be low frequent event in progression of this tumor. For detection of subtle sequence alterations such as point mutations, we performed SSCP analysis of the entire coding region of the p16CDKN2a gene. No altered SSCP patterns were found in 38 clear cell chondrosarcoma specimens. This study reflects the very low incidence of genetic alterations of the p16CDKN2a gene in clear cell chondrosarcoma. Therefore, we conclude that the alteration of the p16CDKN2a gene is not involved significantly in the development of clear cell chondrosarcoma.

journal_name

Int J Oncol

authors

Park YK,Cho CH,Chi SG,Han CS,Ushigome S,Unni KK

doi

10.3892/ijo.19.4.749

keywords:

subject

Has Abstract

pub_date

2001-10-01 00:00:00

pages

749-53

issue

4

eissn

1019-6439

issn

1791-2423

journal_volume

19

pub_type

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