Synthesis of an arrayed sgRNA library targeting the human genome.

abstract：:The sustainable use of common pool resources has become a significant global challenge. It is now widely accepted that specific mechanisms such as community-based management strategies, institutional responses such as resource privatization, information availability and emergent social norms can be used to constrain i...

journal_title：Scientific reports

authors： Osten FBV,Kirley M,Miller T

更新日期：2017-05-23 00:00:00

abstract：:Jasmonates (JAs) are plant-specific key signaling molecules that respond to various stimuli and are involved in the synthesis of secondary metabolites. However, little is known about the JA signal pathway, especially in economically significant medicinal plants. To determine the functions of novel genes that participa...

journal_title：Scientific reports

authors： Ge Q,Zhang Y,Hua WP,Wu YC,Jin XX,Song SH,Wang ZZ

更新日期：2015-09-21 00:00:00

abstract：:Given the prevalence of poor adherence to therapy and the biases of self-reporting across healthcare, we hypothesized that an engaging, personalized therapy may improve adherence and treatment outcomes in the home. We tested this hypothesis in the initial indication of amblyopia, a neurodevelopmental disorder for whic...

journal_title：Scientific reports

authors： Xiao S,Gaier ED,Mazow ML,Stout AU,Travers DA,Angjeli E,Wu HC,Binenbaum G,Hunter DG

更新日期：2020-05-20 00:00:00

abstract：:Membrane fouling, i.e. accumulation of unwanted material on the surface of the membrane is a significant problem in filtration processes since it commonly degrades membrane performance and increases operating costs. Therefore, the advantages of early stage monitoring and control of fouling are widely recognized. In th...

journal_title：Scientific reports

authors： Virtanen T,Reinikainen SP,Lahti J,Mänttäri M,Kallioinen M

更新日期：2018-07-23 00:00:00

abstract：:Zircon of crustal origin found in mantle-derived rocks is of great interest because of the information it may provide about crust recycling and mantle dynamics. Consideration of this requires understanding of how mantle temperatures, notably higher than zircon crystallization temperatures, affected the recycled zircon...

journal_title：Scientific reports

authors： Bea F,Montero P,Palma JFM

更新日期：2018-08-27 00:00:00

abstract：:The physiological, biochemical and functional differences between oxidative and glycolytic muscles play important roles in human metabolic health and in animal meat quality. To explore these differences, we determined the genome-wide landscape of DNA methylomes and their relationship with the mRNA and miRNA transcript...

journal_title：Scientific reports

authors： Shen L,Du J,Xia Y,Tan Z,Fu Y,Yang Q,Li X,Tang G,Jiang Y,Wang J,Li M,Zhang S,Zhu L

更新日期：2016-08-26 00:00:00

abstract：:The cumulative effects of hepatic injury due to hepatitis B virus (HBV) infections and aflatoxin-B1 (AFB1) exposure are the major risk factors of HCC. Understanding early metabolic changes involving these risk factors in an animal model closely resembling human hepatocellular carcinoma (HCC) is critical for biomarker ...

journal_title：Scientific reports

authors： Yaligar J,Teoh WW,Othman R,Verma SK,Phang BH,Lee SS,Wang WW,Toh HC,Gopalan V,Sabapathy K,Velan SS

更新日期：2016-02-02 00:00:00

abstract：:Regular drusen, an accumulation of material below the retinal pigment epithelium (RPE), have long been established as a hallmark early feature of nonneovascular age-related macular degeneration (AMD). Advances in imaging have expanded the phenotype of AMD to include another extracellular deposit, reticular pseudodruse...

journal_title：Scientific reports

authors： Mishra Z,Ganegoda A,Selicha J,Wang Z,Sadda SR,Hu Z

更新日期：2020-06-12 00:00:00

abstract：:Reduced near-infrared reflectance observed in September 1973 in Skylab images of the western flank of Mt. Etna has been interpreted as an eruption precursor of the January 1974 eruption. Until now, it has been unclear when this signal started, whether it was sustained and which process(es) could have caused it. By ana...

journal_title：Scientific reports

authors： Seiler R,Houlié N,Cherubini P

更新日期：2017-03-07 00:00:00

abstract：:Epigenetics alternation of non-genetic variation and genome-wide association study proven allelic variants may associate with insulin secretion in type 2 diabetes (T2D) development. We analyzed promoter DNA methylation array to evaluate the associated with increased susceptibility to T2D (30 cases, 10 controls) and fo...

journal_title：Scientific reports

authors： Chen YT,Lin WD,Liao WL,Tsai YC,Liao JW,Tsai FJ

更新日期：2020-09-30 00:00:00

abstract：:Controlled ovarian stimulation by exogenous gonadotrophins is a key procedure during the in vitro fertilization cycle to obtain a sufficient number of oocytes in humans. Previous studies demonstrated that repeated superovulation had deleterious effects on the ovaries. However, whether repeated superovulation adversely...

journal_title：Scientific reports

authors： Xie JK,Wang Q,Zhang TT,Yin S,Zhang CL,Ge ZJ

更新日期：2016-10-04 00:00:00

abstract：:Low-losses and directionality effects exhibited by High Refractive Index Dielectric particles make them attractive for applications where radiation direction control is relevant. For instance, isolated metallo-dielectric core-shell particles or aggregates (dimers) of High Refractive Index Dielectric particles have bee...

journal_title：Scientific reports

authors： Barreda ÁI,Saleh H,Litman A,González F,Geffrin JM,Moreno F

更新日期：2018-05-22 00:00:00

abstract：:Flat optics, which could planarize and miniaturize the traditional optical elements, possesses the features of extremely low profile and high integration for advanced manipulation of light. Here we proposed and experimentally demonstrated a planar metalens to realize an ultra-long focal length of ~240λ with a large de...

journal_title：Scientific reports

authors： Qin F,Huang K,Wu J,Jiao J,Luo X,Qiu C,Hong M

更新日期：2015-05-06 00:00:00

abstract：:We introduce a method to study the spatial profiles of standing spin waves in ferromagnetic microstructures. The method relies on Nuclear Resonant Scattering of 57Fe using a microfocused beam of synchrotron radiation, the transverse coherence length of which is smaller than the length scale of lateral variations in th...

journal_title：Scientific reports

authors： Gollwitzer J,Bocklage L,Schlage K,Herlitschke M,Wille HC,Leupold O,Adolff CF,Meier G,Röhlsberger R

更新日期：2018-07-26 00:00:00

abstract：:Positron emission tomography (PET) imaging can assist in the early-phase diagnostic and therapeutic evaluation of tumors. Here, we report the radiosynthesis, small animal PET imaging, and biological evaluation of a L-type amino acid transporter 1 (LAT1)-specific PET probe, 18F-FIMP. This probe demonstrates increased t...

journal_title：Scientific reports

authors： Nozaki S,Nakatani Y,Mawatari A,Shibata N,Hume WE,Hayashinaka E,Wada Y,Doi H,Watanabe Y

更新日期：2019-10-31 00:00:00

abstract：:Imaging the fields of magnetic materials provides crucial insight into the physical and chemical processes surrounding magnetism, and has been a key ingredient in the spectacular development of magnetic data storage. Existing approaches using the magneto-optic Kerr effect, x-ray and electron microscopy have limitation...

journal_title：Scientific reports

authors： Simpson DA,Tetienne JP,McCoey JM,Ganesan K,Hall LT,Petrou S,Scholten RE,Hollenberg LC

更新日期：2016-03-14 00:00:00

abstract：:The success of mesenchymal stem cell transplantation is highly dependent on their survival and controlled fate regulation. This study demonstrates that dual-delivery of connective tissue growth factor (CTGF) and fibroblast growth factor 2 (FGF-2) from a core-shell fiber of Silk Fibroin/poly(L-lactic acid-co-ε-caprolac...

journal_title：Scientific reports

authors： Xu R,Zhao H,Muhammad H,Dong M,Besenbacher F,Chen M

更新日期：2017-08-17 00:00:00

abstract：:Gliomas are highly invasive brain tumours characterised by poor prognosis and limited response to therapy. There is an ongoing debate on the therapeutic potential of vaso-modulatory interventions against glioma invasion. Prominent vasculature-targeting therapies involve tumour blood vessel deterioration and normalisat...

journal_title：Scientific reports

authors： Alfonso JC,Köhn-Luque A,Stylianopoulos T,Feuerhake F,Deutsch A,Hatzikirou H

更新日期：2016-11-23 00:00:00

abstract：:We compared the effects of 12-week programs of resistance training (RT), high-intensity interval aerobic training (HIAT), and moderate-intensity continuous aerobic training (MICT). The primary goal was to evaluate the therapeutic effects of the exercise modalities for the management of nonalcoholic fatty liver disease...

journal_title：Scientific reports

authors： Oh S,So R,Shida T,Matsuo T,Kim B,Akiyama K,Isobe T,Okamoto Y,Tanaka K,Shoda J

更新日期：2017-02-22 00:00:00

abstract：:We report on a wavelet based space-scale decomposition method for analyzing the response of living muscle precursor cells (C2C12 myoblasts and myotubes) upon sharp indentation with an AFM cantilever and quantifying their aptitude to sustain such a local shear strain. Beyond global mechanical parameters which are curre...

journal_title：Scientific reports

authors： Streppa L,Ratti F,Goillot E,Devin A,Schaeffer L,Arneodo A,Argoul F

更新日期：2018-06-05 00:00:00

abstract：:We investigated the metabolic outcome of different coping strategies in 129S6/SvEvTac (129Sv) and C57BL/6Ntac (Bl6) strains. Two different batches of male 129Sv and Bl6 mice were used. One batch was not subjected to any behavioral manipulations (home cage control; HCC), whereas the other batch was treated with saline ...

journal_title：Scientific reports

authors： Narvik J,Vanaveski T,Innos J,Philips MA,Ottas A,Haring L,Zilmer M,Vasar E

更新日期：2018-02-21 00:00:00

abstract：:Desmoid tumors (aggressive fibromatosis) are locally invasive soft tissue tumors that lack the ability to metastasize. There are no directed therapies or standard treatment plan, and chemotherapeutics, radiation, and surgery often have temporary effects. The majority of desmoid tumors are related to T41A and S45F muta...

journal_title：Scientific reports

authors： Mercier KA,Al-Jazrawe M,Poon R,Acuff Z,Alman B

更新日期：2018-01-12 00:00:00

abstract：:Obesity and inactivity are major risk factors of feline diabetes mellitus (FDM) and human type II diabetes mellitus (T2DM). In recent years, changes in the gut microbiota have been suggested as a contributing factor to T2DM. Whether the gut microbiota (GM) composition plays a role in FDM remains unknown. The aim of th...

journal_title：Scientific reports

authors： Kieler IN,Osto M,Hugentobler L,Puetz L,Gilbert MTP,Hansen T,Pedersen O,Reusch CE,Zini E,Lutz TA,Bjørnvad CR

更新日期：2019-03-18 00:00:00

abstract：:This work demonstrates the dynamic through-silicon-via (TSV) filling process through staged electrodeposition experiments at different current densities. Different morphologies corresponding to TSV filling results can be obtained by controlling the applied current density. Specifically, a low current density (4 mA/cm2...

journal_title：Scientific reports

authors： Wang F,Zhao Z,Nie N,Wang F,Zhu W

更新日期：2017-04-19 00:00:00

abstract：:Keratin 15 (K15), a type I keratin, which pairs with K5 in epidermis, has been used extensively as a biomarker for stem cells. Two commercial antibodies, LHK15, a mouse monoclonal and EPR1614Y, a rabbit monoclonal, have been widely employed to study K15 expression. Here we report differential reactivity of these antib...

journal_title：Scientific reports

authors： Aldehlawi H,Niemiec KA,Avisetti DR,Lalli A,Teh MT,Waseem A

更新日期：2019-02-13 00:00:00

abstract：:Although technology has triumphed in facilitating routine genome sequencing, new challenges have been created for the data-analyst. Genome-scale surveys of human variation generate volumes of data that far exceed capabilities for laboratory characterization. By incorporating functional annotations as predictors, stati...

journal_title：Scientific reports

authors： Gagliano SA,Ravji R,Barnes MR,Weale ME,Knight J

更新日期：2015-08-24 00:00:00

abstract：:Synthetic fungicides are pesticides widely used in agriculture to control phytopathogenic fungi. The systemicity, persistency and intense application of some of these fungicides, such as boscalid, leads to long periods of exposure for honeybees via contaminated water, pollen and nectar. We exposed adult honeybees in t...

journal_title：Scientific reports

authors： Simon-Delso N,San Martin G,Bruneau E,Hautier L

更新日期：2018-05-08 00:00:00

abstract：:Caffeine is a globally consumed psychostimulant but can be fatal to cells at overdose exposures. Although caspase-dependent apoptosis plays a role in caffeine-induced cell death, the responsible intracellular signalling cascade remains incompletely understood. The cellular slime mould, Dictyostelium discoideum, does n...

journal_title：Scientific reports

authors： Kuwayama H

更新日期：2012-01-01 00:00:00

abstract：:Fat deposition of beef cattle varies between breeds. However, the regulation mechanism is still not elucidated completely at molecular level. In the present study, we comparatively analyzed transcriptome of subcutaneous adipose tissue between Wagyu and Holstein cattle with a significant difference in fat deposition to...

journal_title：Scientific reports

authors： Huang W,Guo Y,Du W,Zhang X,Li A,Miao X

更新日期：2017-07-13 00:00:00

abstract：:The study of factors influencing animal intake can provide a better understanding of the dynamics of the pasture ecosystem and serve as a basis for managing livestock in a more efficient way. We measured different sward surface heights of tall fescue in the process of short-term intake rate of sheep. There was a signi...

journal_title：Scientific reports

authors： Szymczak LS,de Moraes A,Sulc RM,Monteiro ALG,Lang CR,Moraes RF,da Silva DFF,Bremm C,de Faccio Carvalho PC

更新日期：2020-07-16 00:00:00