Abstract：
:Jasmonates (JAs) are plant-specific key signaling molecules that respond to various stimuli and are involved in the synthesis of secondary metabolites. However, little is known about the JA signal pathway, especially in economically significant medicinal plants. To determine the functions of novel genes that participate in the JA-mediated accumulation of secondary metabolites, we examined the metabolomic and transcriptomic signatures from Salvia miltiorrhiza. For the metabolome, 35 representative metabolites showing significant changes in rates of accumulation were extracted and identified. We also screened out 2131 differentially expressed unigenes, of which 30 were involeved in the phenolic secondary metabolic pathway, while 25 were in the JA biosynthesis and signal pathways. Among several MeJA-induced novel genes, SmJAZ8 was selected for detailed functional analysis. Transgenic plants over-expressing SmJAZ8 exhibited a JA-insensitive phenotype, suggesting that the gene is a transcriptional regulator in the JA signal pathway of S. miltiorrhiza. Furthermore, this transgenic tool revealed that JAZ genes have novel function in the constitutive accumulation of secondary metabolites. Based on these findings, we propose that the combined strategy of transcriptomic and metabolomic analyses is valuable for efficient discovery of novel genes in plants.
journal_name
Sci Repjournal_title
Scientific reportsauthors
Ge Q,Zhang Y,Hua WP,Wu YC,Jin XX,Song SH,Wang ZZdoi
10.1038/srep14048subject
Has Abstractpub_date
2015-09-21 00:00:00pages
14048issn
2045-2322pii
srep14048journal_volume
5pub_type
杂志文章abstract：:Little is known regarding the interplays between the mechanical and molecular bases for vein graft restenosis. We elucidated the stenosis initiation using a high-frequency ultrasonic (HFU) echogenicity platform and estimated the endothelium yield stress from von-Mises stress computation to predict the damage locations...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep22147
更新日期：2016-02-26 00:00:00
abstract：:The development of metal-based agents has had a tremendous role in the present progress in cancer chemotherapy. One well-known example of metal-based agents is Schiff based metal complexes, which hold great promise for cancer therapy. Based on the potential of Schiff based complexes for the induction of apoptosis, thi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep09097
更新日期：2015-03-13 00:00:00
abstract：:Damnacanthal, an anthraquinone present in noni plants, targets several tyrosine kinases and has antitumoral effects. This study aims at getting additional insight on the potential of damnacanthal as a natural antitumor compound. The direct effect of damnacanthal on c-Met was tested by in vitro activity assays. Additio...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep08021
更新日期：2015-01-26 00:00:00
abstract：:Experiments on the Ultimatum Game (UG) repeatedly show that people's behaviour is far from rational. In UG experiments, a subject proposes how to divide a pot and the other can accept or reject the proposal, in which case both lose everything. While rational people would offer and accept the minimum possible amount, i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-19503-x
更新日期：2018-01-19 00:00:00
abstract：:Patients with type 1 diabetes have lower bone mineral density and higher risk of fractures. The role of osteoblasts in diabetes-related osteoporosis is well acknowledged whereas the role of osteoclasts (OCLs) is still unclear. We hypothesize that OCLs participate in pathological bone remodeling. We conducted studies i...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30639
更新日期：2016-07-29 00:00:00
abstract：:The success of filamentous fungi in colonizing most natural environments can be largely attributed to their ability to form an expanding interconnected network, the mycelium, or thallus, constituted by a collection of hyphal apexes in motion producing hyphae and subject to branching and fusion. In this work, we charac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-57808-y
更新日期：2020-02-21 00:00:00
abstract：:Non-homologous end joining (NHEJ) is a major DNA double-strand break (DSB) repair mechanism. We characterized here a series of plasmid-based DSB templates that were repaired in Xenopus egg extracts via the canonical, Ku-dependent NHEJ pathway. We showed that the template with compatible ends was efficiently repaired w...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep27797
更新日期：2016-06-21 00:00:00
abstract：:The Brazilian Urban Mobility Policy integrates urban transport with traffic planning, establishing appropriate public policies that indicate the need for a safe and accessible public transport system. The major challenge is the inclusion of the elderly and people with disabilities. In this paper, we quantify the impac...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-80906-w
更新日期：2021-01-12 00:00:00
abstract：:The protein encoded by the TERMINAL FLOWER1 (TFL1) gene maintains indeterminacy in inflorescence meristem to repress flowering, and has undergone multiple duplications. However, basal angiosperms have one copy of a TFL1-like gene, which clusters with eudicot TFL1/CEN paralogs. Functional conservation has been reported...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13645-0
更新日期：2017-11-01 00:00:00
abstract：:On-surface reactions based on Ullmann coupling are known to proceed on coinage-metal substrates (e.g. Au, Ag, Cu), with the chemistry of the surface strongly influencing the reaction progression. In addition, the topography of the surface may be expected to affect the local adsorption geometry of the reactants as well...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-13315-1
更新日期：2017-11-06 00:00:00
abstract：:The Vacuole Membrane Protein 1 -VMP1- is a pancreatitis-associated transmembrane protein whose expression triggers autophagy in several human diseases. In the current study, we unveil the mechanism through which this protein induces autophagosome formation in mammalian cells. We show that VMP1 autophagy-related functi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep01055
更新日期：2013-01-01 00:00:00
abstract：:Osteosarcoma pulmonary metastases are typically treated with resection and/or chemotherapy. We hypothesize that stereotactic radiosurgery (SRS) can be an alternative to surgery that can achieve high rates of local control with limited toxicity. From January 2005 to December 2013, 73 patients who developed pulmonary me...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-14521-7
更新日期：2017-12-12 00:00:00
abstract：:It is generally assumed that an investment into cognitive abilities and their associated cost is particularly beneficial for long-lived species, as a prolonged lifespan allows to recoup the initial investment. However, ephemeral organisms possess astonishing cognitive abilities too. Invertebrates, for example, are cap...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51652-5
更新日期：2019-10-23 00:00:00
abstract：:Perinatal depression involves interplay between individual chronic and acute disease burdens, biological and psychosocial environmental and behavioural factors. Here we explored the predictive potential of specific psycho-socio-demographic characteristics for antenatal and postpartum depression symptoms and contributi...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-30874-z
更新日期：2018-08-24 00:00:00
abstract：:The use of cardiac troponins (cTn) is the gold standard for diagnosing myocardial infarction. Independent of myocardial infarction (MI), however, sex, age and kidney function affect cTn levels. Here we developed a method to adjust cTnI levels for age, sex, and renal function, maintaining a unified cut-off value such a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-26120-1
更新日期：2018-05-24 00:00:00
abstract：:Lipid mediators play pivotal roles in colorectal cancer and colitis, but only a limited member of the phospholipase A2 (PLA2) subtypes, which lie upstream of various lipid mediators, have been implicated in the positive or negative regulation of these diseases. Clinical and biochemical evidence suggests that secreted ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-12434-z
更新日期：2017-09-25 00:00:00
abstract：:The overuse of antibiotics results in the development of antibiotic resistance and limits the useful life of these drugs in fighting bacteria, including Aeromonas hydrophila, a well-known opportunistic pathogen that causes serious infections in fish and other animals. In this study, we investigated the adaptive resist...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39413
更新日期：2016-12-19 00:00:00
abstract：:Limited continuous replenishment of the mineralization medium is a restriction for in-situ solution-based remineralization of hypomineralized body tissues. Here, we report a process that generated amine-functionalized mesoporous silica nanoparticles for sustained release of biomimetic analog-stabilized amorphous calci...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep11199
更新日期：2015-06-08 00:00:00
abstract：:Quantum key distribution involving decoy-states is a significant application of quantum information. By using three-intensity decoy-states of single-photon-added coherent sources, we propose a practically realizable scheme on quantum key distribution which approaches very closely the ideal asymptotic case of an infini...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep15130
更新日期：2015-10-14 00:00:00
abstract：:Recent studies have established that single nucleotide polymorphisms are sufficient to build accurate predictive models of gene expression. Gamazon, et al., found that gene expression values predicted from cis neighborhood SNPs show statistical association with disease status. In this work, we remove the cis neighborh...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep39360
更新日期：2016-12-20 00:00:00
abstract：:Axillary lymph node status is an important prognostic factor for breast cancer patients and sentinel lymph node biopsy (SLNB) is a less invasive surgical proxy. We examined if consecutively derived molecular subtypes from primary breast cancers provide additional predictive value for SLNB status. 1556 patients with a ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-021-81538-4
更新日期：2021-01-26 00:00:00
abstract：:Accurate species identification is fundamental when recording ecological data. However, the ability to correctly identify organisms visually is rarely questioned. We investigated how experts and non-experts compared in the identification of bumblebees, a group of insects of considerable conservation concern. Experts a...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33634
更新日期：2016-09-20 00:00:00
abstract：:Bursaphelenchus xylophilus is a quarantined migratory endoparasite known to cause severe economic losses in pine forest ecosystems. The study presents the nematicidal effects of halogenated indoles on B. xylophilus and their action mechanisms. 5-Iodoindole and abamectin (positive control) at low concentration (10 µg/m...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-07074-2
更新日期：2017-07-28 00:00:00
abstract：:The molecular bases of the Frank-Starling law of the heart and of its cellular counterpart, the length dependent activation (LDA), are largely unknown. However, the recent discovery of the thick filament activation, a second pathway beside the well-known calcium mediated thin filament activation, is promising for eluc...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-018-36706-4
更新日期：2019-02-04 00:00:00
abstract：:Successful tissue repair requires the activities of myeloid cells such as monocytes and macrophages that guide the progression of inflammation and healing outcome. Immunoregenerative materials leverage the function of endogenous immune cells to orchestrate complex mechanisms of repair; however, a deeper understanding ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-017-00477-1
更新日期：2017-03-27 00:00:00
abstract：:Thyroid hormone (TH) has long been believed to play a minor role in male reproduction. However, evidences from experimental model of thyrotoxicosis or hypothyroidism suggests its role in spermatogenesis. Cellular action of TH requires membrane transport via specific transporters such as monocarboxylate transporter 8 (...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-020-67594-2
更新日期：2020-07-07 00:00:00
abstract：:Without any root contact with the soil, epiphytic bryophytes must experience and explore poor, patchy, and heterogeneous habitats; while, the nitrogen (N) uptake and use strategies of these organisms remain uncharacterized, which obscures their roles in the N cycle. To investigate the N sources, N preferences, and res...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep30408
更新日期：2016-07-27 00:00:00
abstract：:Pancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive and lethal malignancies. Long non-coding RNAs (lncRNAs) are a novel class of non-protein-coding transcripts that have been implicated in cancer biogenesis and prognosis. By repurposing microarray probes, we herein analysed the lncRNA expression...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/srep33535
更新日期：2016-09-15 00:00:00
abstract：:The distinguishing of the multiphoton quantum interference effect from the classical one forms one of the most important issues in modern quantum mechanics and experimental quantum optics. For a long time, the two-photon interference (TPI) of correlated photons has been recognized as a pure quantum effect that cannot ...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-54504-4
更新日期：2019-12-05 00:00:00
abstract：:We study the capillary retraction of a Newtonian semi-infinite liquid filament through analytical methods. We derive a long-time asymptotic-state expansion for the filament profile using a one-dimensional free-surface slender cylindrical flow model based on the three-dimensional axisymmetric Navier-Stokes equations. T...
journal_title：Scientific reports
pub_type： 杂志文章
doi：10.1038/s41598-019-51824-3
更新日期：2019-10-29 00:00:00
