Abstract:
:The breeding systems of many organisms are cryptic and difficult to investigate with observational data, yet they have profound effects on a species' ecology, evolution, and genome organization. Genomic approaches offer a novel, indirect way to investigate breeding systems, specifically by studying the transmission of genetic information from parents to offspring. Here we exemplify this method through an assessment of self-fertilization vs. automictic parthenogenesis in Daphnia magna. Self-fertilization reduces heterozygosity by 50% compared to the parents, but under automixis, whereby two haploid products from a single meiosis fuse, the expected heterozygosity reduction depends on whether the two meiotic products are separated during meiosis I or II (i.e., central vs. terminal fusion). Reviewing the existing literature and incorporating recombination interference, we derive an interchromosomal and an intrachromosomal prediction of how to distinguish various forms of automixis from self-fertilization using offspring heterozygosity data. We then test these predictions using RAD-sequencing data on presumed automictic diapause offspring of so-called nonmale producing strains and compare them with "self-fertilized" offspring produced by within-clone mating. The results unequivocally show that these offspring were produced by automixis, mostly, but not exclusively, through terminal fusion. However, the results also show that this conclusion was only possible owing to genome-wide heterozygosity data, with phenotypic data as well as data from microsatellite markers yielding inconclusive or even misleading results. Our study thus demonstrates how to use the power of genomic approaches for elucidating breeding systems, and it provides the first demonstration of automictic parthenogenesis in Daphnia.
journal_name
Geneticsjournal_title
Geneticsauthors
Svendsen N,Reisser CM,Dukić M,Thuillier V,Ségard A,Liautard-Haag C,Fasel D,Hürlimann E,Lenormand T,Galimov Y,Haag CRdoi
10.1534/genetics.115.179879subject
Has Abstractpub_date
2015-11-01 00:00:00pages
1143-55issue
3eissn
0016-6731issn
1943-2631pii
genetics.115.179879journal_volume
201pub_type
杂志文章相关文献
GENETICS文献大全abstract::All living organisms require nutrient minerals for growth and have developed mechanisms to acquire, utilize, and store nutrient minerals effectively. In the aqueous cellular environment, these elements exist as charged ions that, together with protons and hydroxide ions, facilitate biochemical reactions and establish ...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.112.147207
更新日期:2013-03-01 00:00:00
abstract::In both pedigree linkage studies and in population-based association studies there has been much interest in the use of modern dense genetic marker data to infer segments of gene identity by descent (ibd) among individuals not known to be related, to increase power and resolution in localizing genes affecting complex ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.137570
更新日期:2012-04-01 00:00:00
abstract::Genetic analysis of 131 independent transpositions of the tetracycline-resistance element Tn10 from a single site in phage P22 into the histidine operon of Salmonella typhimurium reveals that Tn10 insertions are not randomly distributed along this chromosomal target. The insertions occur in 22 different "clusters"; in...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1979-08-01 00:00:00
abstract::Ribosome biogenesis requires at least 18 putative ATP-dependent RNA helicases in Saccharomyces cerevisiae. To explore the functional environment of one of these putative RNA helicases, Dbp6p, we have performed a synthetic lethal screen with dbp6 alleles. We have previously characterized the nonessential Rsa1p, whose n...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.4.1687
更新日期:2004-04-01 00:00:00
abstract::C4 and CYP21 are two adjacent, but functionally unrelated genes residing in the middle of the mammalian major histocompatibility complex (Mhc). The C4 gene codes for the fourth component of the complement cascade, whereas the CYP21 gene specifies an enzyme (cytochrome P450c21) of the glucocorticoid and mineralocortico...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-05-01 00:00:00
abstract::Secondary trisomics and telotrisomics representing the 12 chromosomes of rice were isolated from the progenies of primary trisomics. A large population of each primary trisomic was grown. Plants showing variation in gross morphology compared to the primary trisomics and disomic sibs were selected and analyzed cytologi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::We performed a comparative study of four subfamilies of olfactory receptor genes first identified in the dog to assess changes in the gene family during mammalian evolution, and to begin linking the dog genetic map to that of humans. The human subfamilies were localized to chromosomes 7, 11, and 19. The two subfamilie...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Currently used tests of Hardy-Weinberg proportions do not take into account the nature of the alternative hypothesis, which is generally a heterozygote deficiency. Different exact tests, appropriate for small sample size and large number of alleles, are proposed in this perspective, and their properties are evaluated ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-08-01 00:00:00
abstract::Mutation frequency and specificity were determined as a function of age in nuclear DNA from liver, bladder, and brain of Big Blue lacI transgenic mice aged 1.5-25 months. Mutations accumulated with age in liver and accumulated more rapidly in bladder. In the brain a small initial increase in mutation frequency was obs...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-03-01 00:00:00
abstract::Mutations identified in zebrafish genetic screens allow the dissection of a wide array of problems in vertebrate biology. Most screens have examined mutations induced by treatment of spermatogonial (premeiotic) cells with the chemical mutagen N-ethyl-N-nitrosourea (ENU). Treatment of postmeiotic gametes with ENU induc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-05-01 00:00:00
abstract::Immunosenescence, the age-related decline in immune system function, is a general hallmark of aging. While much is known about the cellular and physiological changes that accompany immunosenescence, we know little about the genetic influences on this phenomenon. In this study we combined age-specific measurements of b...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.140640
更新日期:2012-07-01 00:00:00
abstract::During meiosis homologous chromosomes replicate once, pair, experience recombination, and undergo two rounds of segregation to produce haploid meiotic products. The rec8(+), rec10(+), and rec11(+) genes of the fission yeast Schizosaccharomyces pombe exhibit similar specificities for meiotic recombination and rec8(+) i...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-09-01 00:00:00
abstract::Two large, stable populations (Texas and Japan) of Drosophila melanogaster were surveyed at 21 allozyme loci on the second and third chromosomes and for chromosomal gene arrangements on those two chromosomes. Over 220 independent gametes were sampled from each population. The types and frequencies of the surveyed gene...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-11-01 00:00:00
abstract::Mutation of a CCG sequence in the 5'-untranslated region of the mitochondrially encoded cytochrome b mRNA in Saccharomyces cerevisiae results in destabilization of the message and respiratory deficiency of the mutant strain. This phenotype mimics that of a mutation in the nuclear CBP1 gene. Here it is shown that overe...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.036467
更新日期:2005-11-01 00:00:00
abstract::Biological robustness, the ability of an organism to maintain a steady-state output as genetic or environmental inputs change, is critical for proper development. MicroRNAs have been implicated in biological robustness mechanisms through their post-transcriptional regulation of genes and gene networks. Previous resear...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.179184
更新日期:2015-08-01 00:00:00
abstract::USING A NEWLY DEVELOPED PLATING SYSTEM, WE HAVE MEASURED CELL SURVIVAL AND THE FREQUENCIES OF VARIATION IN AN INHERITED TRAIT AFTER TREATMENT OF SOYBEAN CELL SUSPENSIONS WITH DIFFERENT MUTAGENS: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-Methyl-N'-nitro-N-nitroso-guanidine (MNNG), hycanthone (1-{[2...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-09-01 00:00:00
abstract::Three sex-transforming genes (ix, tra-D, and dsx) in D. melanogaster were examined with respect to possible interactions with the NSR strain SR-spirochetes. The SR-spirochetes exerted a lethal effect on XY but not on XX individuals, regardless of whether they were phenotypically males, intersexes, or females. These re...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-01-01 00:00:00
abstract::The relationship between multidimensional form of the adult mouse mandible and body size is examined from an ontogenetic perspective. The origin and ontogeny of phenotypic correlations are described in terms of genetic and environmental covariance patterns between adult skeletal morphology and growth in body weight. D...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-11-01 00:00:00
abstract::The genetic basis of stochastic variation within a defined environment, and the consequences of such micro-environmental variance for fitness are poorly understood . Using a multigenerational breeding design in Drosophila serrata, we demonstrated that the micro-environmental variance in a set of morphological wing tra...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.199075
更新日期:2017-08-01 00:00:00
abstract::The eight enzymes of the tricarboxylic acid (TCA) cycle are encoded by at least 15 different nuclear genes in Saccharomyces cerevisiae. We have constructed a set of yeast strains defective in these genes as part of a comprehensive analysis of the interactions among the TCA cycle proteins. The 15 major TCA cycle genes ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Early embryogenesis in Drosophila melanogaster is controlled by maternal gene products, which are deposited in the egg during oogenesis. It is not well understood how maternal gene expression is controlled during germline development. pipsqueak (psq) is a complex locus that encodes several nuclear protein variants con...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.121624
更新日期:2011-02-01 00:00:00
abstract::Competition among domesticated plants or animals can have a dramatic negative impact on yield of a stand or farm. The usual quantitative genetic model ignores these competitive interactions and could result in seriously incorrect breeding decisions and acerbate animal well-being. A general solution to this problem is ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.035956
更新日期:2005-07-01 00:00:00
abstract::Genetic divergence in the form of the mandible is examined in ten inbred strains of mice. Several univariate and multivariate genetic distance estimates are given for the morphological data and these estimates are compared to measures of genealogical and molecular divergence. Highly significant divergence occurs among...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-09-01 00:00:00
abstract::RNAs are localized by microtubule-based pathways to both the anterior and posterior poles of the developing Drosophila oocyte. We describe a new gene, wispy, required for localization of mRNAs to both poles of the egg. Embryos from wispy mothers arrest development after abnormal oocyte meiosis and failure of pronuclei...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-04-01 00:00:00
abstract::Sixteen inbred or partially inbred strains of rabbits were investigated for electrophoretic and quantitative variations of alpha-1-antitrypsin (A-1-AT). We found interindividual differences in the electrophoretic A-1-AT patterns as well as quantitative differences in the concentrations of A-1-AT and the serum trypsin-...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::A genetic strategy identified Salmonella typhimurium strains carrying large (> 40 kb) Tn 10-catalyzed inversions; the inverted segments were characterized by XbaI digestion and pulsed field gel electrophoresis. Two size classes of large inversions were found. More than half of the inversions extended 40-80 kb either c...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-03-01 00:00:00
abstract::A number of fundamental processes comprise the cell division cycle, including spindle formation, chromosome segregation, and cytokinesis. Our current understanding of these processes has benefited from the isolation and analysis of mutants, with the meiotic divisions in the male germline of Drosophila being particular...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::Diversity in insect pigmentation, encompassing a wide range of colors and spatial patterns, is among the most noticeable features distinguishing species, individuals, and body regions within individuals. In holometabolous species, a significant portion of such diversity can be attributed to the melanin synthesis genes...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.186684
更新日期:2016-05-01 00:00:00
abstract::Sponsored by the National Science Foundation and the U.S. Department of Agriculture, a wheat genome sequencing workshop was held November 10-11, 2003, in Washington, DC. It brought together 63 scientists of diverse research interests and institutions, including 45 from the United States and 18 from a dozen foreign cou...
journal_title:Genetics
pub_type:
doi:10.1534/genetics.104.034769
更新日期:2004-10-01 00:00:00
abstract::Epistasis contributes significantly to intrapopulation variation in floral morphology, development time, and male fitness components of Mimulus guttatus. This is demonstrated with a replicated line-cross experiment involving slightly over 7000 plants. The line-cross methodology is based on estimates for means. It thus...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.041525
更新日期:2005-12-01 00:00:00