Abstract:
:Mutation frequency and specificity were determined as a function of age in nuclear DNA from liver, bladder, and brain of Big Blue lacI transgenic mice aged 1.5-25 months. Mutations accumulated with age in liver and accumulated more rapidly in bladder. In the brain a small initial increase in mutation frequency was observed in young animals; however, no further increase was observed in adult mice. To investigate the origin of mutations, the mutational spectra for each tissue and age were determined. DNA sequence analysis of mutant lacI transgenes revealed no significant changes in mutational specificity in any tissue at any age. The spectra of mutations found in aging animals were identical to those in younger animals, suggesting that they originated from a common set of DNA lesions manifested during DNA replication. The data also indicated that there were no significant age-related mutational changes due to oxidative damage, or errors resulting from either changes in the fidelity of DNA polymerase or the efficiency of DNA repair. Hence, no evidence was found to support hypotheses that predict that oxidative damage or accumulation of errors in nuclear DNA contributes significantly to the aging process, at least in these three somatic tissues.
journal_name
Geneticsjournal_title
Geneticsauthors
Stuart GR,Oda Y,de Boer JG,Glickman BWkeywords:
subject
Has Abstractpub_date
2000-03-01 00:00:00pages
1291-300issue
3eissn
0016-6731issn
1943-2631journal_volume
154pub_type
杂志文章相关文献
GENETICS文献大全abstract::Cell surface immunoglobulin superfamily (IgSF) proteins play important roles in the development and function of the nervous system . Here we define the role of a Caenorhabditis elegans IgSF protein, RIG-3, in the function of the AVA command interneuron. This study reveals that RIG-3 regulates the abundance of the glut...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302872
更新日期:2020-01-01 00:00:00
abstract::The molecular basis of the maternally inherited, heteroplasmic NCS2 mutant of maize was investigated. Analysis of the NCS2 mtDNA showed that it closely resembles the progenitor cmsT mitochondrial genome, except that the mutant genome contains a fused nad4-nad7 gene and is deleted for the small fourth exon of nad4. The...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1994-11-01 00:00:00
abstract::Trans-species polymorphism, meaning the presence of alleles in different species that are more similar to each other than they are to alleles in the same species, has been found at loci associated with vegetative incompatibility in filamentous fungi. If individuals differ at one or more of these loci (termed het for h...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-06-01 00:00:00
abstract::The evolution of domesticated maize from its wild ancestor teosinte is a dramatic example of the effect of human selection on agricultural crops. Maize has one dominant axis of growth, whereas teosinte is highly branched. The axillary branches in maize are short and feminized whereas the axillary branches of teosinte ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-12-01 00:00:00
abstract::Salmonella typhimurium can normally use glycine as a serine source to support the growth of serine auxotrophs. This reaction was presumed to occur by the reversible activity of the enzyme, serine transhydroxymethylase (E. C. 2. 1. 2. 1; L-serine: tetrahydrofolic-5, 10 transhydroxymethylase), which is responsible for g...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-06-01 00:00:00
abstract::While bacteria divide clonally, horizontal gene transfer followed by homologous recombination is now recognized as an important contributor to their evolution. However, the details of how the competition between clonality and recombination shapes genome diversity remains poorly understood. Using a computational model,...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300061
更新日期:2017-09-01 00:00:00
abstract::Extensive length polymorphism and heteroplasmy (multiple forms within an individual) of the D-loop region are observed in mitochondrial DNA of the white sturgeon (Acipenser transmontanus). The nucleotide sequence of this region, for both a short and a long form, shows that the differences are due to variable numbers o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-01-01 00:00:00
abstract::Recombination and chromosome synapsis bring homologous chromosomes together, creating chiasmata that ensure accurate disjunction during reductional division. SPO13 is a key gene required for meiosis I (MI) reductional segregation, but dispensable for recombination, in Saccharomyces cerevisiae. Absence of SPO13 leads t...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Orthologous positions of 55 genes associated with height in four human populations were located on the bovine genome. Single nucleotide polymorphisms close to eight of these genes were significantly associated with stature in cattle (Bos taurus and Bos indicus). This suggests that these genes may contribute to control...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.123943
更新日期:2011-03-01 00:00:00
abstract::One of the most important parameters in population genetics is theta = 4Ne mu where Ne is the effective population size and mu is the rate of mutation per gene per generation. We study two related problems, using the maximum likelihood method and the theory of coalescence. One problem is the potential improvement of a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-08-01 00:00:00
abstract::Trichome development in Arabidopsis thaliana is a well-characterized model for the study of plant cell differentiation. Two genes that play an essential role in the initiation of trichome development are GL1 and TTG. Mutations in either gene prevent the initiation of most trichomes. The GL1 gene encodes a myb-related ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1999-04-01 00:00:00
abstract::A coalescent theory for a sample of DNA sequences from a partially selfing diploid population and an algorithm for simulating such samples are developed in this article. Approximate formulas are given for the expectation and the variance of the number of segregating sites in a sample of k sequences from n individuals....
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-08-01 00:00:00
abstract::Neurospora crassa has been a model organism for the study of circadian clocks for the past four decades. Among natural accessions of Neurospora crassa, there is significant variation in clock phenotypes. In an attempt to investigate natural allelic variants contributing to quantitative variation, we used a quantitativ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.077958
更新日期:2007-12-01 00:00:00
abstract::Infantile neuronal ceroid lipofuscinosis (INCL) is a pediatric neurodegenerative disease caused by mutations in the human CLN1 gene. CLN1 encodes palmitoyl-protein thioesterase 1 (PPT1), suggesting an important role for the regulation of palmitoylation in normal neuronal function. To further elucidate Ppt1 function, w...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.067983
更新日期:2007-05-01 00:00:00
abstract::Thirty-four independent nonviable c-locus mutations (types cal, albino lethal and cas, albino subvital), derived from radiation experiments, were tested for involvement of nearby markers tp, Mod-2, sh-1, and Hbb: 10, 22, and 2 involved, respectively, none of these markers, Mod-2 alone, and Mod-2 plus sh-1. When classi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-03-01 00:00:00
abstract::In this paper we study the process of allele frequency change in finite populations with overlapping generations with the purpose of evaluating the possibility of estimating the effective size from observations of temporal frequency shifts of selectively neutral alleles. Focusing on allele frequency changes between su...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-02-01 00:00:00
abstract::The lin-31 gene is required for the proper specification of vulval cell fates in the nematode Caenorhabditis elegans and encodes a member of the winged-helix family of transcription factors. Members of this important family have been identified in many organisms and are known to bind specific DNA targets involved in a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-12-01 00:00:00
abstract::The rdgB mutants depend on recombinational repair of double-strand breaks. To assess other consequences of rdgB inactivation in Escherichia coli, we isolated RdgB-dependent mutants. All transposon inserts making cells dependent on RdgB inactivate genes of double-strand break repair, indicating that chromosomal fragmen...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.051144
更新日期:2006-02-01 00:00:00
abstract::Gene silencing mediated by dsRNA (RNAi) can persist for multiple generations in Caenorhabditis elegans (termed RNAi inheritance). Here we describe the results of a forward genetic screen in C. elegans that has identified six factors required for RNAi inheritance: GLH-1/VASA, PUP-1/CDE-1, MORC-1, SET-32, and two novel ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198812
更新日期:2017-07-01 00:00:00
abstract::Two alternative hypotheses attribute different benefits to codon-anticodon adaptation. The first assumes that protein production is rate limited by both initiation and elongation and that codon-anticodon adaptation would result in higher elongation efficiency and more efficient and accurate protein production, especia...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.172106
更新日期:2015-02-01 00:00:00
abstract::This paper presents the results of a study of linkage disequilibrium between five polymorphic enzyme genes located on chromosome 3 of D. melanogaster. Three sets of chromosomes were examined: two represented samples from successive years of the same natural population, and one came from a large laboratory population. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-02-01 00:00:00
abstract::The no-on-transient-A (nonA) gene of Drosophila melanogaster influences vision, courtship song, and viability. The nonA-encoded polypeptide is inferred to bind single-stranded nucleic acids. Although sequence-analysis of NONA implies that it belongs to a special interspecific family of this protein type, it does conta...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00
abstract::A two-locus model is used to analyze the effectiveness of a mixture of insecticides in delaying resistance, compared to the use of the insecticides singly. The effects of factors such as recombination, effective dominance, initial value of allele frequencies and initial value of linkage disequilibrium are considered. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-04-01 00:00:00
abstract::We show that major chromosomal rearrangements can occur upon T-DNA transformation of Arabidopsis thaliana. In the ACL4 line, two T-DNA insertion loci were found; one is a tandem T-DNA insert in a head-to-head orientation, and the other is a truncated insert with only the left part of the T-region. The four flanking DN...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::Estimating the fixation probability of a beneficial mutation has a rich history in theoretical population genetics. Typically, to attain mathematical tractability, we assume that generation times are fixed, while the number of offspring per individual is stochastic. However, fixation probabilities are extremely sensit...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.072009
更新日期:2007-07-01 00:00:00
abstract::The extent to which epistasis contributes to adaptation, population differentiation, and speciation is a long-standing and important problem in evolutionary genetics. Using recombinant inbred (RI) lines of Arabidopsis thaliana grown under natural field conditions, we have examined the genetic architecture of fitness-c...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.046078
更新日期:2005-12-01 00:00:00
abstract::We explore the effects of demography and linkage on a maximum-likelihood (ML) method for estimating selection and mutation parameters in a reversible mutation model. This method assumes free recombination between sites and a randomly mating population of constant size and uses information from both polymorphic and mon...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.122150
更新日期:2010-12-01 00:00:00
abstract::Mutations in the genealogy of the sequences in a random sample from a population can be classified as external and internal. External mutations are mutations that occurred in the external branches and internal mutations are mutations that occurred in the internal branches of the genealogy. Under the assumption of sele...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::In order to define more precisely the most proximal portion of chromosome 3R in Drosophila melanogaster, several new chromosome aberrations involving this region have been recovered and analyzed. These new arrangements were recovered as induced reversions of two dominant mutations, ANTPNs and dsxD, located in the regi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::Chromatin insulators separate active from repressed chromatin domains. In yeast the RNA pol III transcription machinery bound to tRNA genes function with histone acetylases and chromatin remodelers to restrict the spread of heterochromatin. Our results collectively demonstrate that binding of TFIIIC is necessary for i...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.106203
更新日期:2009-09-01 00:00:00