Abstract:
:USING A NEWLY DEVELOPED PLATING SYSTEM, WE HAVE MEASURED CELL SURVIVAL AND THE FREQUENCIES OF VARIATION IN AN INHERITED TRAIT AFTER TREATMENT OF SOYBEAN CELL SUSPENSIONS WITH DIFFERENT MUTAGENS: ethyl methanesulfonate (EMS), methyl methanesulfonate (MMS), N-Methyl-N'-nitro-N-nitroso-guanidine (MNNG), hycanthone (1-{[2-(diethylamino) ethyl] amino}-4-(hydroxymethyl)-9H-thioxanthen-9-one and ultraviolet light (UV).-The heritable variation selected for displays a phenotype of rapid growth on maltose as carbon source. The marker is stable in the absence of maltose, and prolonged growth of variant cells on sucrose has not shown reversions to slow growth. Doubling time in suspension cultures is decreased from 100 hr to ca. 30 hr by the mutation. Both wild-type and variant cells grow on sucrose with a 24-hr doubling time. Thus, lethality after mutagen treatment can be estimated rapidly by growth on sucrose, whereas variants are scored on maltose medium. The spontaneous frequency of variants was 1.2 x 10(-7); induced frequencies ranged from a low of 3.6 x 10(-5) for EMS to a high of 10(-3) for hycanthone. The high frequency of variants induced by hycanthone, a frame-shift mutagen, and the observation that UV induces variants in haploid cells with much higher frequency than in diploid cells suggests a recessive mutation.
journal_name
Geneticsjournal_title
Geneticsauthors
Weber G,Lark KGsubject
Has Abstractpub_date
1980-09-01 00:00:00pages
213-22issue
1eissn
0016-6731issn
1943-2631journal_volume
96pub_type
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