Abstract:
:Mutations identified in zebrafish genetic screens allow the dissection of a wide array of problems in vertebrate biology. Most screens have examined mutations induced by treatment of spermatogonial (premeiotic) cells with the chemical mutagen N-ethyl-N-nitrosourea (ENU). Treatment of postmeiotic gametes with ENU induces specific-locus mutations at a higher rate than premeiotic regimens, suggesting that postmeiotic mutagenesis protocols could be useful in some screening strategies. Whereas there is extensive evidence that ENU induces point mutations in premeiotic cells, the range of mutations induced in postmeiotic zebrafish germ cells has been less thoroughly characterized. Here we report the identification and analysis of five mutations induced by postmeiotic ENU treatment. One mutation, snh(st1), is a translocation involving linkage group (LG) 11 and LG 14. The other four mutations, oep(st2), kny(st3), Df(LG 13)(st4), and cyc(st5), are deletions, ranging in size from less than 3 cM to greater than 20 cM. These results show that germ cell stage is an important determinant of the type of mutations induced. The induction of chromosomal rearrangements may account for the elevated frequency of specific-locus mutations observed after treatment of postmeiotic gametes with ENU.
journal_name
Geneticsjournal_title
Geneticsauthors
Imai Y,Feldman B,Schier AF,Talbot WSkeywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
261-72issue
1eissn
0016-6731issn
1943-2631journal_volume
155pub_type
杂志文章相关文献
GENETICS文献大全abstract::Additive genetic variance in natural populations is commonly estimated using mixed models, in which the covariance of the genetic effects is modeled by a genetic similarity matrix derived from a dense set of markers. An important but usually implicit assumption is that the presence of any nonadditive genetic effect in...
journal_title:Genetics
pub_type: 信件
doi:10.1534/genetics.115.177212
更新日期:2016-01-01 00:00:00
abstract::A lethal locus (l(2)br7;35B6-10), near Adh on chromosome arm 2L of D. melanogaster, is identified with Plunkett's dominant suppressor of Hairless (H). Of eight new alleles, seven act as dominant suppressors of H, the eighth is a dominant enhancer of H. One of the suppressor alleles is both a leaky lethal and a weak s...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-07-01 00:00:00
abstract::The Duchenne muscular dystrophy (Dmd) locus lies in a region of the X chromosome that experiences a high rate of recombination and is thus expected to be relatively unaffected by the effects of selection on nearby genes. To provide a picture of nucleotide variability at a high-recombination locus in humans, we sequenc...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-08-01 00:00:00
abstract::Double-strand DNA breaks can be repaired by any of several alternative mechanisms that differ greatly in the nature of the final repaired products. We used a reporter construct, designated "Repair reporter 3" (Rr3), to measure the relative usage of these pathways in Drosophila germ cells. The method works by creating ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.050138
更新日期:2006-02-01 00:00:00
abstract::Malaria control projects based on the introduction and spread of transgenes into mosquito populations depend on the extent of isolation between those populations. On the basis of the distribution of paracentric inversions, Anopheles gambiae has been subdivided into five subspecific chromosomal forms. Estimating gene f...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026534
更新日期:2005-01-01 00:00:00
abstract::The organization of the mitochondrial genome in plants is not well understood. In maize mitochondrial DNA (mtDNA) several subgenomic circular molecules as well as an abundant fraction of linear molecules have been seen by electron microscopy. It has been hypothesized that the circular molecules are the genetic entitie...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::Using conditional probabilities and moment-generating matrices, I derived approximate algebraic equations that give expectations of gene frequency, population mean, gene frequency variance within lines, or heterozygosity, and gene frequency variance between lines, or drift, for repeated cycles of recurrent selection i...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1980-07-01 00:00:00
abstract::The standard slipped-strand mispairing (SSM) model for the formation of variable number tandem repeats (VNTRs) proposes that a few tandem repeats, produced by chance mutations, provide the "raw material" for VNTR expansion. However, this model is unlikely to explain the formation of VNTRs with long motifs (e.g., minis...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-07-01 00:00:00
abstract::This study concerns the problem of odor receptor gene choice in the fruit fly Drosophila melanogaster. From a family of 60 Odor receptor genes, only one or a small number are selected for expression by each olfactory receptor neuron. Little is known about how an olfactory receptor neuron selects a receptor, or how the...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.117622
更新日期:2010-09-01 00:00:00
abstract::As one of the world's most important food crops, the potato (Solanum tuberosum L.) has spurred innovation in autotetraploid genetics, including in the use of SNP arrays to determine allele dosage at thousands of markers. By combining genotype and pedigree information with phenotype data for economically important trai...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.300685
更新日期:2018-05-01 00:00:00
abstract::Molecular heterogeneity in human breast cancer has challenged diagnosis, prognosis, and clinical treatment. It is well known that molecular subtypes of breast tumors are associated with significant differences in prognosis and survival. Assuming that the differences are attributed to subtype-specific pathways, we then...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.180125
更新日期:2015-12-01 00:00:00
abstract::Epistasis contributes significantly to intrapopulation variation in floral morphology, development time, and male fitness components of Mimulus guttatus. This is demonstrated with a replicated line-cross experiment involving slightly over 7000 plants. The line-cross methodology is based on estimates for means. It thus...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.041525
更新日期:2005-12-01 00:00:00
abstract::Population structure leads to systematic patterns in measures of mean relatedness between individuals in large genomic data sets, which are often discovered and visualized using dimension reduction techniques such as principal component analysis (PCA). Mean relatedness is an average of the relationships across locus-s...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301747
更新日期:2019-01-01 00:00:00
abstract::Pseudo-homothallism is a reproductive strategy elected by some fungi producing heterokaryotic sexual spores containing genetically different but sexually compatible nuclei. This lifestyle appears as a compromise between true homothallism (self-fertility with predominant inbreeding) and complete heterothallism (with ex...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.113.159988
更新日期:2014-05-01 00:00:00
abstract::In the absence of a vg+ gene, extensive cell death occurs in third instar imaginal discs, which results in a complete loss of adult wing margin structures. Essentially all molecularly characterized vg alleles are associated with deletions or insertions of DNA into the vg locus. These alterations reduce or eliminate a ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1990-08-01 00:00:00
abstract::An SSR-based linkage map was constructed in Brassica rapa. It includes 113 SSR, 87 RFLP, and 62 RAPD markers. It consists of 10 linkage groups with a total distance of 1005.5 cM and an average distance of 3.7 cM. SSRs are distributed throughout the linkage groups at an average of 8.7 cM. Synteny between B. rapa and a ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.038968
更新日期:2006-05-01 00:00:00
abstract::Two alternate transcripts of the single copy Alcohol dehydrogenase (Adh) gene accumulate with developmental specificity in all of 12 species of Hawaiian picture-winged Drosophila which have been examined. Relative to the paradigm species D. affinidisjuncta, the Adh transcript normally restricted to larvae is found to ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-10-01 00:00:00
abstract::In vivo haploid induction (HI) triggered by pollination with special intraspecific genotypes, called inducers, is unique to Zea maysL. within the plant kingdom and has revolutionized maize breeding during the last decade. However, the molecular mechanisms underlying HI in maize are still unclear. To investigate the ge...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.184234
更新日期:2016-04-01 00:00:00
abstract::Codon usage bias in Drosophila melanogaster genes has been attributed to negative selection of those codons whose cellular tRNA abundance restricts rates of mRNA translation. Previous studies, which involved limited numbers of genes, can now be compared against analyses of the entire gene complements of 12 Drosophila ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.070466
更新日期:2007-11-01 00:00:00
abstract::Primary cilia have essential roles in transducing signals in eukaryotes. At their core is the ciliary axoneme, a microtubule-based structure that defines cilium morphology and provides a substrate for intraflagellar transport. However, the extent to which axonemal microtubules are specialized for sensory cilium functi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.110.116996
更新日期:2010-07-01 00:00:00
abstract::There are two naturally occurring functional alleles of the recombination hotspot cog, which is located 3.5 kb from the his-3 locus of Neurospora crassa. The presence of the cog+ allele in a cross significantly increases recombination in the his-3 region compared to a cross homozygous for the cog allele. Data obtained...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.025080
更新日期:2004-07-01 00:00:00
abstract::Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.026617
更新日期:2004-09-01 00:00:00
abstract::The small ovary gene (sov) is required for the development of the Drosophila ovary. Six EMS-induced recessive alleles have been identified. Hypomorphic alleles are female sterile and have no effect on male fertility, whereas more severe mutations result in lethality. The female-sterile alleles produce a range of mutan...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-03-01 00:00:00
abstract::We describe here a new variant of transposon Tn10 especially adapted for transposon analysis of cloned yeast genes; it can equally well be used for analysis of prokaryotic genes. We have applied this element to analysis of the LEU2, RAD50, and CDC48 genes of Saccharomyces cerevisiae. This transposon, nicknamed mini-Tn...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1987-06-01 00:00:00
abstract::We propose a simple algorithm for estimating the number of nucleotide differences between a pair of RNA or DNA sequences through comparison of their RNAse A mismatch cleavage patterns. In the RNAse A mismatch cleavage technique two or more sample sequences are hybridized to the same RNA probe, the hybrids are partiall...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-12-01 00:00:00
abstract::The identification of a second structural gene mutation at the feline arylsulfatase B locus (MPS VIb) provided the opportunity to investigate the expression of allelism by characterization of the residual enzymatic activity in feline mucopolysaccharidosis VI, an animal analogue of human Maroteaux-Lamy syndrome. Mating...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1985-08-01 00:00:00
abstract::137Cs-gamma irradiation of bacteriophage T4 induces large deletions plus a variety of types of point mutations. All mutations arise with single-hit kinetics, and all by a misrepair process. The estimated point mutation rate is 1.5 X 10(-9) per locus per rad. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-04-01 00:00:00
abstract::Restorer-of-fertility (Rf) alleles for S-type cytoplasmic male sterility (CMS-S) are prevalent in Mexican races of maize and teosinte. Forty-five Rf alleles from 26 races of maize and 6 Rf alleles from different accessions of teosinte were found to be homozygous viable, consistent with the hypothesis that they are nat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.2.959
更新日期:2004-02-01 00:00:00
abstract::In a subdivided population, the interaction between natural selection and stochastic change in allele frequency is affected by the occurrence of local extinction and subsequent recolonization. The relative importance of selection can be diminished by this additional source of stochastic change in allele frequency. Res...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.2.1105
更新日期:2004-02-01 00:00:00
abstract::An extending axon growth cone is subjected to attractant and repellent cues. It is not clear how these growth cones discriminate the two opposing forces and select their projection paths. Here, we report that in the Drosophila nerve cord the growth cones of longitudinal tracts are subjected to attraction by the Netrin...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.036863
更新日期:2005-05-01 00:00:00