Abstract:
:Cyclin E together with its kinase partner Cdk2 is a critical regulator of entry into S phase. To identify novel genes that regulate the G1- to S-phase transition within a whole animal we made use of a hypomorphic cyclin E mutation, DmcycEJP, which results in a rough eye phenotype. We screened the X and third chromosome deficiencies, tested candidate genes, and carried out a genetic screen of 55,000 EMS or X-ray-mutagenized flies for second or third chromosome mutations that dominantly modified the DmcycEJP rough eye phenotype. We have focused on the DmcycEJP suppressors, S(DmcycEJP), to identify novel negative regulators of S-phase entry. There are 18 suppressor gene groups with more than one allele and several genes that are represented by only a single allele. All S(DmcycEJP) tested suppress the DmcycEJP rough eye phenotype by increasing the number of S phases in the postmorphogenetic furrow S-phase band. By testing candidates we have identified several modifier genes from the mutagenic screen as well as from the deficiency screen. DmcycEJP suppressor genes fall into the classes of: (1) chromatin remodeling or transcription factors; (2) signaling pathways; and (3) cytoskeletal, (4) cell adhesion, and (5) cytoarchitectural tumor suppressors. The cytoarchitectural tumor suppressors include scribble, lethal-2-giant-larvae (lgl), and discs-large (dlg), loss of function of which leads to neoplastic tumors and disruption of apical-basal cell polarity. We further explored the genetic interactions of scribble with S(DmcycEJP) genes and show that hypomorphic scribble mutants exhibit genetic interactions with lgl, scab (alphaPS3-integrin--cell adhesion), phyllopod (signaling), dEB1 (microtubule-binding protein--cytoskeletal), and moira (chromatin remodeling). These interactions of the cytoarchitectural suppressor gene, scribble, with cell adhesion, signaling, cytoskeletal, and chromatin remodeling genes, suggest that these genes may act in a common pathway to negatively regulate cyclin E or S-phase entry.
journal_name
Geneticsjournal_title
Geneticsauthors
Brumby A,Secombe J,Horsfield J,Coombe M,Amin N,Coates D,Saint R,Richardson Hdoi
10.1534/genetics.104.026617keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
227-51issue
1eissn
0016-6731issn
1943-2631pii
168/1/227journal_volume
168pub_type
杂志文章相关文献
GENETICS文献大全abstract::Recent investigations of evolution in heterogeneous environments have begun to accommodate genetic and environmental complexity typical of natural populations. Theoretical studies demonstrate that evolution of polygenic characters depends heavily on the genetic interdependence of the expression of traits in the differ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::Meiosis was analyzed cytogenetically in autotetraploids of Arabidopsis, including both established lines and newly generated autotetraploid plants. Fluorescent in situ hybridization with 5S and 45S rDNA probes was used to identify the different chromosomes at metaphase I of meiosis. Multivalents were observed frequent...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-11-01 00:00:00
abstract::Site-specific recombinases are widely used tools for analysis of genetics, development, and cell biology, and many schemes have been devised to alter gene expression by recombinase-mediated DNA rearrangements. Because the FRT and lox target sites for the commonly used FLP and Cre recombinases are asymmetrical, and mus...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303394
更新日期:2020-08-01 00:00:00
abstract::A mouse homolog of the Drosophila pair-rule gene Odd Oz (Odz4) maps to the critical region of the l7Rn3 locus on mouse chromosome 7. Here we show that Odz4 is an excellent candidate for this allelic series because (1) it spans the entire critical region, (2) the phenotypes correlate with embryonic expression, (3) the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.034967
更新日期:2005-01-01 00:00:00
abstract::Whole-mount fluorescence in situ hybridization (FISH) was applied to Arabidopsis thaliana seedlings to determine the three-dimensional (3D) interphase chromosome territory (CT) arrangement and heterochromatin location within the positional context of entire tissues or in particular cell types of morphologically well-p...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.073270
更新日期:2007-06-01 00:00:00
abstract::We investigated genetic polymorphism in the Caenorhabditis elegans srh and str chemoreceptor gene families, each of which consists of approximately 300 genes encoding seven-pass G-protein-coupled receptors. Almost one-third of the genes in each family are annotated as pseudogenes because of apparent functional defects...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.035329
更新日期:2005-04-01 00:00:00
abstract::Neurons and glia display remarkable morphological plasticity, and remodeling of glia may facilitate neuronal shape changes. The molecular basis and control of glial shape changes is not well understood. In response to environmental stress, the nematode Caenorhabditis elegans enters an alternative developmental state, ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.137786
更新日期:2012-04-01 00:00:00
abstract::The F(1) hybrids of Nicotiana suaveolens (subgenus Petunioides, 2n = 32) and N. glutinosa (subgenus Tabacum, 2n = 24), were examined during their development, from seedlings to mature plants. It was observed that in the hybrids, there was a progressive change of dominant N. glutinosa morphological characteristics towa...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1973-04-01 00:00:00
abstract::DNA extracts of several rosy-mutation-bearing strains were associated with large insertions and deletions in a defined region of the molecular map believed to include the rosy locus DNA. Large-scale, intragenic mapping experiments were carried out that localized these mutations within the boundaries of the previously ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-04-01 00:00:00
abstract::Hybridization between related species results in the formation of an allopolyploid with multiple subgenomes. These subgenomes will each contain complete, yet evolutionarily divergent, sets of genes. Like a diploid hybrid, allopolyploids will have two versions, or homeoalleles, for every gene. Partial functional redund...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301851
更新日期:2019-03-01 00:00:00
abstract::Recombinant inbred lines (RIL) derived from multiple inbred strains can serve as a powerful resource for the genetic dissection of complex traits. The use of such multiple-strain RIL requires a detailed knowledge of the haplotype structure in such lines. Broman (2005) derived the two- and three-point haplotype probabi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.064063
更新日期:2007-03-01 00:00:00
abstract::Hybrid incompatibility can result from gene misregulation produced by divergence in trans-acting regulatory factors and their cis-regulatory targets. However, change in trans-acting factors may be constrained by pleiotropy, which would in turn limit the evolution of incompatibility. We employed a mechanistically expli...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.171397
更新日期:2014-12-01 00:00:00
abstract::The yeast genetics community has embraced genomic biology, and there is a general understanding that obtaining a full encyclopedia of functions of the approximately 6000 genes is a worthwhile goal. The yeast literature comprises over 40,000 research papers, and the number of yeast researchers exceeds the number of gen...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.107.074468
更新日期:2007-05-01 00:00:00
abstract::Results of immunogenetic, immunochemical and physicochemical investigations on two serum allotypes of swine are reported. The allotypes, designated Lpr1 and Lpr2, have been identified by specific alloprecipitins in agar gel. Genetic studies indicate that the allotypes are specified by two codominant autosomal allelic ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::There are two naturally occurring functional alleles of the recombination hotspot cog, which is located 3.5 kb from the his-3 locus of Neurospora crassa. The presence of the cog+ allele in a cross significantly increases recombination in the his-3 region compared to a cross homozygous for the cog allele. Data obtained...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.025080
更新日期:2004-07-01 00:00:00
abstract::Mating behavior in adult male nematodes can be assayed by mating efficiency, i.e., the number of cross progeny sired by males under standard conditions. Mutant males from 220 strains, representing most of the known complementation groups of C. elegans, have been examined for mating efficiency and for anatomical abnorm...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-01-01 00:00:00
abstract::Levels of neutral variation are influenced by background selection and hitchhiking. The relative contribution of these evolutionary forces to the distribution of neutral variation is still the subject of ongoing debates. Using 133 microsatellites, we determined levels of variability on X chromosomes and autosomes in A...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-01-01 00:00:00
abstract::The agouti locus on mouse chromosome 2 encodes a secreted cysteine-rich protein of 131 amino acids that acts as a molecular switch to instruct the melanocyte to make either yellow pigment (phaeomelanin) or black pigment (eumelanin). Mutations that up-regulate agouti expression are dominant to those causing decreased e...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1995-05-01 00:00:00
abstract::Immunosenescence, the age-related decline in immune system function, is a general hallmark of aging. While much is known about the cellular and physiological changes that accompany immunosenescence, we know little about the genetic influences on this phenomenon. In this study we combined age-specific measurements of b...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.112.140640
更新日期:2012-07-01 00:00:00
abstract::Although neurons are highly polarized, how neuronal polarity is generated remains poorly understood. An evolutionarily conserved inositol-producing enzyme myo-inositol monophosphatase (IMPase) is essential for polarized localization of synaptic molecules in Caenorhabditis elegans and can be inhibited by lithium, a dru...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.137844
更新日期:2012-06-01 00:00:00
abstract::Whereas nonlinear relationships between genes are acknowledged, there exist only a few methods for estimating nonlinear gene coexpression networks or gene regulatory networks (GCNs/GRNs) with common deficiencies. These methods often consider only pairwise associations between genes, and are, therefore, poorly capable ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.120.303186
更新日期:2020-07-01 00:00:00
abstract::In eukaryotic cells, the organization of genomic DNA into chromatin regulates many biological processes, from the control of gene expression to the regulation of chromosome segregation. The proper maintenance of this structure upon cell division is therefore of prime importance during development for the maintenance o...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.190785
更新日期:2017-01-01 00:00:00
abstract::Drosophila melanogaster heterochromatin protein 2 (HP2) interacts with heterochromatin protein 1 (HP1). In polytene chromosomes, HP2 and HP1 colocalize at the chromocenter, telomeres, and the small fourth chromosome. We show here that HP2 is present in the arms as well as the centromeric regions of mitotic chromosomes...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.057604
更新日期:2006-11-01 00:00:00
abstract::Amoebae of the Colonia (CL) strain, which normally produce plasmodia in clones, are shown to cross with mt3 and mt4 amoebae under appropriate conditions. Strains carrying mt3 and mt4 in a CL genetic background were constructed and used in the genetic analysis of mutants isolated in CL. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-12-01 00:00:00
abstract::Log-linear analysis of contingency tables is applied to trihybrid backcross data to estimate linkage and viability. Whereas nonadditive viability differences perturb recombination estimates in the classical analysis, this statistical procedure yields maximum likelihood crossover frequency estimates in the presence of ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1981-09-01 00:00:00
abstract::Heparan sulfates (HS) are linear polysaccharides with complex modification patterns, which are covalently bound via conserved attachment sites to core proteins to form heparan sulfate proteoglycans (HSPGs). HSPGs regulate many aspects of the development and function of the nervous system, including cell migration, mor...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.198739
更新日期:2017-08-01 00:00:00
abstract::Argonaute2 (Ago2) is a rapidly evolving nuclease in the Drosophila melanogaster RNA interference (RNAi) pathway that targets viruses and transposable elements in somatic tissues. Here we reconstruct the history of Ago2 duplications across the D. obscura group and use patterns of gene expression to infer new functional...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.116.192336
更新日期:2016-10-01 00:00:00
abstract::The additive genomic variance in linear models with random marker effects can be defined as a random variable that is in accordance with classical quantitative genetics theory. Common approaches to estimate the genomic variance in random-effects linear models based on genomic marker data can be regarded as estimating ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302324
更新日期:2019-10-01 00:00:00
abstract::The effect of full-sib inbreeding on competitive male-mating ability (CImale symbol) in Drosophila melanogaster was investigated in two experiments. In the first, five inbred lines (with reserves) were assessed up to 18 generations. Linear inbreeding depression, of 5.9% per 10% increase in homozygosity, was observed. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1984-04-01 00:00:00
abstract::In Drosophila there exist several examples of gene expression that can be modified by an interaction between alleles; this effect is known as transvection. The inference that alleles interact comes from the observations that homologous chromosomes pair in mitotically dividing cells, and that chromosome rearrangements ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1996-05-01 00:00:00