Abstract:
:Heparan sulfates (HS) are linear polysaccharides with complex modification patterns, which are covalently bound via conserved attachment sites to core proteins to form heparan sulfate proteoglycans (HSPGs). HSPGs regulate many aspects of the development and function of the nervous system, including cell migration, morphology, and network connectivity. HSPGs function as cofactors for multiple signaling pathways, including the Wnt-signaling molecules and their Frizzled receptors. To investigate the functional interactions among the HSPG and Wnt networks, we conducted genetic analyses of each, and also between these networks using five cellular migrations in the nematode Caenorhabditis elegans We find that HSPG core proteins act genetically in a combinatorial fashion dependent on the cellular contexts. Double mutant analyses reveal distinct redundancies among HSPGs for different migration events, and different cellular migrations require distinct heparan sulfate modification patterns. Our studies reveal that the transmembrane HSPG SDN-1/Syndecan functions within the migrating cell to promote cellular migrations, while the GPI-linked LON-2/Glypican functions cell nonautonomously to establish the final cellular position. Genetic analyses with the Wnt-signaling system show that (1) a given HSPG can act with different Wnts and Frizzled receptors, and that (2) a given Wnt/Frizzled pair acts with different HSPGs in a context-dependent manner. Lastly, we find that distinct HSPG and Wnt/Frizzled combinations serve separate functions to promote cellular migration and establish position of specific neurons. Our studies suggest that HSPGs use structurally diverse glycans in coordination with Wnt-signaling pathways to control multiple cellular behaviors, including cellular and axonal migrations and, cellular positioning.
journal_name
Geneticsjournal_title
Geneticsauthors
Saied-Santiago K,Townley RA,Attonito JD,da Cunha DS,Díaz-Balzac CA,Tecle E,Bülow HEdoi
10.1534/genetics.116.198739subject
Has Abstractpub_date
2017-08-01 00:00:00pages
1951-1967issue
4eissn
0016-6731issn
1943-2631pii
genetics.116.198739journal_volume
206pub_type
杂志文章相关文献
GENETICS文献大全abstract::The conserved histone variant H2A.Z fulfills many functions by being an integral part of the nucleosomes placed at specific regions of the genome. Telomeres cap natural ends of chromosomes to prevent their recognition as double-strand breaks. At yeast telomeres, H2A.Z prevents the spreading of silent chromatin into pr...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.095547
更新日期:2008-12-01 00:00:00
abstract::Thyroid hormone has pleiotropic effects on cochlear development, and genomic variation influences the severity of associated hearing deficits. DW/J-Pou1f1dw/dw mutant mice lack pituitary thyrotropin, which causes severe thyroid hormone deficiency and profound hearing impairment. To assess the genetic complexity of pro...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.130633
更新日期:2011-10-01 00:00:00
abstract::The linkage disequilibrium pattern analysis is a method that allows one to detect present and past events of selection. We applied it to our data on the inversions of Drosophila mediopunctata and to published data of Drosophila subobscura, which are systems probably under complex modes of selection. The method gave me...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-11-01 00:00:00
abstract::Florida teosinte from Honduras and Guanajuato teosinte have most of their knobs internally located and a common inversion in the short arm of chromosome 8. Teosintes from northern Guatemala, Huixta and Monajil, have all their knobs terminally located and do not have any inversions. Therefore, Florida teosinte from Hon...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1976-08-01 00:00:00
abstract::We develop the theory for computing the joint frequency spectra of alleles in two closely related species. We allow for arbitrary population growth in both species after they had a common ancestor. We focus on the case in which a single chromosome is sequenced from one of the species. We use classical diffusion theory...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.070730
更新日期:2007-09-01 00:00:00
abstract::This paper describes a perturbation experiment on the frequency of the F and S Alcohol dehydrogenase (Adh) alleles of D. melanogaster. Fifty-four iso-female lines set up from three wild populations and with initial F frequencies of either 0.25, 0.50 or 0.75 were maintained on standard laboratory food medium at 22 degr...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1982-01-01 00:00:00
abstract::We have analyzed the requirements for the multi sex combs (mxc) gene during development to gain further insight into the mechanisms and developmental processes that depend on the important trans-regulators forming the Polycomb group (PcG) in Drosophila melanogaster. mxc is allelic with the tumor suppressor locus letha...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-08-01 00:00:00
abstract::Restriction fragment length polymorphisms in mitochondrial DNA (mtDNA) were used to reconstruct evolutionary relationships of conspecific populations in four species of freshwater fish-Amia calva, Lepomis punctatus, L. gulosus, and L. microlophus. A suite of 14-17 endonucleases was employed to assay mtDNAs from 305 sp...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1986-08-01 00:00:00
abstract::Detecting and quantifying the differences in individual genomes (i.e., genotyping), plays a fundamental role in most modern bioinformatics pipelines. Many scientists now use reduced representation next-generation sequencing (NGS) approaches for genotyping. Genotyping diploid individuals using NGS is a well-studied fie...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301468
更新日期:2018-11-01 00:00:00
abstract::Codon usage bias in Drosophila melanogaster genes has been attributed to negative selection of those codons whose cellular tRNA abundance restricts rates of mRNA translation. Previous studies, which involved limited numbers of genes, can now be compared against analyses of the entire gene complements of 12 Drosophila ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.107.070466
更新日期:2007-11-01 00:00:00
abstract::A natural population of Culex tritaeniorhynchus was sampled to test the efficacy of a proposed method to detect and measure concealed variability. Thirty-nine lethals and ten recessive visible mutations were uncovered out of a total of 125 chromosomes tested. ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-06-01 00:00:00
abstract::Mutations in the genealogy of the sequences in a random sample from a population can be classified as external and internal. External mutations are mutations that occurred in the external branches and internal mutations are mutations that occurred in the internal branches of the genealogy. Under the assumption of sele...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-03-01 00:00:00
abstract::Establishing durable disease resistance in agricultural crops, where much of the plant defense is provided through effector-R gene interactions, is complicated by the ability of pathogens to overcome R gene resistance by losing the corresponding effector gene. Many proposed methods to maintain disease resistance in th...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.2.693
更新日期:2004-02-01 00:00:00
abstract::To identify in vivo pathways that compensate for impaired proliferating cell nuclear antigen (PCNA or Pol30p in yeast) activity, we performed a synthetic lethal screen with the yeast pol30-104 mutation. We identified nine mutations that display synthetic lethality with pol30-104; three mutations affected the structura...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-02-01 00:00:00
abstract::Critically shortened telomeres can be subjected to DNA repair events that generate end-to-end chromosome fusions. The resulting dicentric chromosomes can enter breakage-fusion-bridge cycles, thereby impeding elucidation of the structures of the initial fusion events and a mechanistic understanding of their genesis. Cu...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.089920
更新日期:2008-10-01 00:00:00
abstract::Condensins are evolutionarily conserved protein complexes that are required for chromosome segregation during cell division and genome organization during interphase. In Caenorhabditis elegans, a specialized condensin, which forms the core of the dosage compensation complex (DCC), binds to and represses X chromosome t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.119.302254
更新日期:2019-07-01 00:00:00
abstract::To study the interdependence of Gcr1p and Rap1p, we prepared a series of synthetic regulatory sequences that contained various numbers and combinations of CT-boxes (Gcr1p-binding sites) and RPG-boxes (Rap1p-binding sites). The ability of the synthetic oligonucleotides to function as regulatory sequences was tested usi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1997-10-01 00:00:00
abstract::Following the advent of a gene targeting technique in Drosophila, different methods have been developed to modify the Drosophila genome. The initial demonstration of gene targeting in flies used an ends-in method, which generates a duplication of the target locus. The duplicated locus can then be efficiently reduced t...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.030882
更新日期:2004-11-01 00:00:00
abstract::Protein serine/threonine phosphatase type 1 (PP1) has been found in all eukaryotes examined to date and is involved in the regulation of many cellular functions, including glycogen metabolism, muscle contraction, and mitosis. In Drosophila, four genes code for the catalytic subunit of PP1 (PP1c), three of which belong...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.069914
更新日期:2007-05-01 00:00:00
abstract::The fixation probability is determined when population size and selection change over time and differs from Kimura's result, with long-term implications for a population. It is found that changes in population size are not equivalent to the corresponding changes in selection and can result in less drift than anticipat...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.129288
更新日期:2011-08-01 00:00:00
abstract::In order to define more precisely the most proximal portion of chromosome 3R in Drosophila melanogaster, several new chromosome aberrations involving this region have been recovered and analyzed. These new arrangements were recovered as induced reversions of two dominant mutations, ANTPNs and dsxD, located in the regi...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-08-01 00:00:00
abstract::In diploid Saccharomyces cerevisiae cells, bud-site selection is determined by two cortical landmarks, Bud8p and Bud9p, at the distal and proximal poles, respectively. Their localizations depend on the multigenerational proteins Rax1p/Rax2p. Many genes involved in bud-site selection were identified previously by genom...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.128231
更新日期:2011-08-01 00:00:00
abstract::Understanding the initiation of meiosis and the relationship of this event with other key cytogenetic processes are major goals in studying the genetic control of meiosis in higher plants. Our genetic and structural analysis of two mutant alleles of the ameiotic1 gene (am1 and am1-praI) suggest that this locus plays a...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1993-12-01 00:00:00
abstract::Demonstrating that naturally occurring enzyme polymorphisms significantly impact metabolic pathway flux is a fundamental step in examining the possible adaptive significance of such polymorphisms. In earlier studies of the glucose-6-phosphate dehydrogenase (G6PD) polymorphism in Drosophila melanogaster, we used two di...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-11-01 00:00:00
abstract::The availability of genomewide dense markers brings opportunities and challenges to breeding programs. An important question concerns the ways in which dense markers and pedigrees, together with phenotypic records, should be used to arrive at predictions of genetic values for complex traits. If a large number of marke...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.109.101501
更新日期:2009-05-01 00:00:00
abstract::Heritable silencing effects are gene suppression phenomena that can persist for generations after induction. In the majority of RNAi experiments conducted in Caenorhabditis elegans, the silencing response results in a hypomorphic phenotype where the effects recede after the F1 generation. F2 and subsequent generations...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.108.089433
更新日期:2008-11-01 00:00:00
abstract::Race-cultivar specialization during the interaction of the basidiomycete smut pathogen Ustilago hordei with its barley host was described in the 1940s. Subsequent genetic analyses revealed the presence of dominant avirulence genes in the pathogen that conform to the gene-for-gene theory. This pathosystem therefore pre...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.166.1.99
更新日期:2004-01-01 00:00:00
abstract::To investigate the genetic basis of maize adaptation to temperate climate, collections of 375 inbred lines and 275 landraces, representative of American and European diversity, were evaluated for flowering time under short- and long-day conditions. The inbred line collection was genotyped for 55 genomewide simple sequ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.105.048603
更新日期:2006-04-01 00:00:00
abstract::The organization of the mitochondrial genome in plants is not well understood. In maize mitochondrial DNA (mtDNA) several subgenomic circular molecules as well as an abundant fraction of linear molecules have been seen by electron microscopy. It has been hypothesized that the circular molecules are the genetic entitie...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1991-06-01 00:00:00
abstract::The net rate of mutation to deleterious but nonlethal alleles and the sizes of effects of these mutations are of great significance for many evolutionary questions. Here we describe three replicate experiments in which mutations have been accumulated on chromosome 3 of Drosophila melanogaster by means of single-male b...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.103.025262
更新日期:2004-06-01 00:00:00