Abstract：
:Critically shortened telomeres can be subjected to DNA repair events that generate end-to-end chromosome fusions. The resulting dicentric chromosomes can enter breakage-fusion-bridge cycles, thereby impeding elucidation of the structures of the initial fusion events and a mechanistic understanding of their genesis. Current models for the molecular basis of fusion of critically shortened, uncapped telomeres rely on PCR assays that typically capture fusion breakpoints created by direct ligation of chromosome ends. Here we use independent approaches that rely on distinctive features of Caenorhabditis elegans to study the frequency of direct end-to-end chromosome fusion in telomerase mutants: (1) holocentric chromosomes that allow for genetic isolation of stable end-to-end fusions and (2) unique subtelomeric sequences that allow for thorough PCR analysis of samples of genomic DNA harboring multiple end-to-end fusions. Surprisingly, only a minority of end-to-end fusion events resulted from direct end joining with no additional genome rearrangements. We also demonstrate that deficiency for the C. elegans Ku DNA repair heterodimer does not affect telomere length or cause synthetic effects in the absence of telomerase.
journal_name
Geneticsjournal_title
Geneticsauthors
Lowden MR,Meier B,Lee TW,Hall J,Ahmed Sdoi
10.1534/genetics.108.089920subject
Has Abstractpub_date
2008-10-01 00:00:00pages
741-54issue
2eissn
0016-6731issn
1943-2631pii
genetics.108.089920journal_volume
180pub_type
杂志文章相关文献
GENETICS文献大全abstract：:The idealized individual in many fungal species is a haploid self-sterile hermaphrodite that may be propagated by asexually produced spores or that may reproduce sexually. In field populations, polymorphism occurs for female-sterile/hermaphrodite status, and female-sterile mutants, which function only as males during ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-10-01 00:00:00
abstract：:The distribution of four X-linked mutants (G6PD, Deutan, Protan and Xg) among lowland and once highly malarial populations of Sardinia discloses a clear-cut example of linkage disequiligrium between two of them (G6PD and Protan). In the same populations the distribution of G6PD-deficiency versus colorblindness of the ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-05-01 00:00:00
abstract：:Ten mutations are described that transform genotypic hermaphrodites of the nematode Caenorhabditis elegans into phenotypic males. These fall into three autosomal complementation groups, termed tra-1, tra-2, and tra-3. Two alleles of tra-1 produce almost complete transformation, to a fertile male phenotype; such transf...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-06-01 00:00:00
abstract：:We have performed an F2 genetic screen to identify lethal mutations within the 63E-64A genomic region. We have isolated 122 mutations in 20 different complementation groups. Of these groups, 16 are represented by multiple alleles. We have also established that the Rop and Ras2 genes are located within the 63E-64A geno...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-04-01 00:00:00
abstract：:Plant breeders make selection decisions based on multiple traits, such as yield, plant height, flowering time, and disease resistance. A commonly used approach in multi-trait genomic selection is index selection, which assigns weights to different traits relative to their economic importance. However, classical index ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.120.303305
更新日期：2020-08-01 00:00:00
abstract：:The Saccharomyces cerevisiae GAL/MEL regulon genes are normally induced within minutes of galactose addition, but gal3 mutants exhibit a 3-5-day induction lag. We have discovered that this long-term adaptation (LTA) phenotype conferred by gal3 is complemented by multiple copies of the GAL1 gene. Based on this result a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-06-01 00:00:00
abstract：:Stress-induced mutagenesis is a collection of mechanisms observed in bacterial, yeast, and human cells in which adverse conditions provoke mutagenesis, often under the control of stress responses. Control of mutagenesis by stress responses may accelerate evolution specifically when cells are maladapted to their enviro...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.100735
更新日期：2009-05-01 00:00:00
abstract：:Regulatory networks provide control over complex cell behavior in all kingdoms of life. Here we describe a statistical model, based on representing proteins as collections of domains or motifs, which predicts unknown molecular interactions within these biological networks. Using known protein-protein interactions of S...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-11-01 00:00:00
abstract：:LARK is an essential Drosophila RNA-binding protein of the RNA recognition motif (RRM) class that functions during embryonic development and for the circadian regulation of adult eclosion. LARK protein contains three consensus RNA-binding domains: two RRM domains and a retroviral-type zinc finger (RTZF). To show that ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-09-01 00:00:00
abstract：:Data are presented for 2,393 progeny from a number of crosses related to a study in ecological genetics of the Burnsi and Kandiyohi polymorphisms in natural populations of the leopard frog, Rana pipiens. No significant differences in viability were found between wild-type homozygotes (+/+) and Burnsi heterozygotes (B/...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1972-01-01 00:00:00
abstract：:To identify the factors (selective or mutational) that affect the distribution of transposable elements (TEs) within a genome, it is necessary to compare the pattern of newly arising element insertions to the pattern of element insertions that have been fixed in a population. To do this, we analyzed the distribution o...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-11-01 00:00:00
abstract：:This study addresses three questions about the properties of recombination hotspots in Saccharomyces cerevisiae: How much DNA is required for double-strand-break (DSB) site recognition? Do naturally occurring DSB sites compete with each other in meiotic recombination? What role does the sequence located at the sites o...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-09-01 00:00:00
abstract：:Malaria control projects based on the introduction and spread of transgenes into mosquito populations depend on the extent of isolation between those populations. On the basis of the distribution of paracentric inversions, Anopheles gambiae has been subdivided into five subspecific chromosomal forms. Estimating gene f...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.026534
更新日期：2005-01-01 00:00:00
abstract：:No proper statistical test is available for the evaluation of deviation of a single homozygous genotype from Hardy-Weinberg equilibrium (HWE) proportion. We propose a 1-d.f. chi2-test. The power of the proposed test is favorable compared to existing HWE testing procedures. The applications of this test are discussed. ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.105.043190
更新日期：2005-07-01 00:00:00
abstract：:A method is proposed for estimating the intensity of overdominant selection scaled by the effective population size, S = 2Ns, from allele frequencies. The method is based on the assumption that, with strong overdominant selection, allele frequencies are nearly at their deterministic equilibrium values and that, to a f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2000-12-01 00:00:00
abstract：:DNA extracts of several rosy-mutation-bearing strains were associated with large insertions and deletions in a defined region of the molecular map believed to include the rosy locus DNA. Large-scale, intragenic mapping experiments were carried out that localized these mutations within the boundaries of the previously ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-04-01 00:00:00
abstract：:To identify in vivo pathways that compensate for impaired proliferating cell nuclear antigen (PCNA or Pol30p in yeast) activity, we performed a synthetic lethal screen with the yeast pol30-104 mutation. We identified nine mutations that display synthetic lethality with pol30-104; three mutations affected the structura...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-02-01 00:00:00
abstract：:An analysis of mortality is undertaken in two breeds of pigs: Danish Landrace and Yorkshire. Zero-inflated and standard versions of hierarchical Poisson, binomial, and negative binomial Bayesian models were fitted using Markov chain Monte Carlo (MCMC). The objectives of the study were to investigate whether there is s...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.110759
更新日期：2010-01-01 00:00:00
abstract：:The 73AD salivary chromosome region of Drosophila melanogaster was subjected to mutational analysis in order to (1) generate a collection of chromosome breakpoints that would allow a correlation between the genetic, cytological and molecular maps of the region and (2) define the number and gross organization of comple...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-08-01 00:00:00
abstract：:Active endogenous transposable elements, useful tools for gene isolation, have not been reported from any legume species. An active transposable element was suggested to reside in the W4 locus that governs flower color in soybean. Through biochemical and molecular analyses of several revertants of the w4-m allele, we ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.107904
更新日期：2010-01-01 00:00:00
abstract：:Mismatch repair of palindromic loops in the presence or absence of single-base mismatches was investigated in wild-type and mismatch-binding defective mutant Chinese hamster ovary cells. Recombination intermediates with a maximum heteroduplex DNA (hDNA) region of 697 bp contained a centrally located, phenotypically si...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-03-01 00:00:00
abstract：:As millions of single-nucleotide polymorphisms (SNPs) have been identified and high-throughput genotyping technologies have been rapidly developed, large-scale genomewide association studies are soon within reach. However, since a genomewide association study involves a large number of SNPs it is therefore nearly impo...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.106.060491
更新日期：2006-11-01 00:00:00
abstract：:The term "micro-evo-devo" refers to the combined study of the genetic and developmental bases of natural variation in populations and the evolutionary forces that have shaped this variation. It thus represents a synthesis of the fields of evolutionary developmental biology and population genetics. As has been pointed ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.113.156463
更新日期：2013-11-01 00:00:00
abstract：:The island model with stochastically variable migration rate and immigrant gene frequency is investigated. It is supposed that the migration rate and the immigrant gene frequency are independent of each other in each generation, and each of them is independently and identically distributed in every generation. The tre...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-01-01 00:00:00
abstract：:l(3)S12 is a vital locus whose function is required in embryos, early larvae, late pupae and oogenesis. We have identified a cold-sensitive allele, l(3)S12(3), and characterized conditional misexpression of the gene associated with this mutation as well as with several euchromatic insertions of l(3)S12+ transposons. S...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-05-01 00:00:00
abstract：:Adaptive dynamics formalism demonstrates that, in a constant environment, a continuous trait may first converge to a singular point followed by spontaneous transition from a unimodal trait distribution into a bimodal one, which is called "evolutionary branching." Most previous analyses of evolutionary branching have b...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.112.144980
更新日期：2013-01-01 00:00:00
abstract：:Though much has been learned about the process of ovarian follicle maturation through studies of oogenesis in both vertebrate and invertebrate systems, less is known about how follicles form initially. In Drosophila, two somatic follicle stem cells (FSCs) in each ovariole give rise to all polar cells, stalk cells, and...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.109538
更新日期：2010-02-01 00:00:00
abstract：:Diversity in insect pigmentation, encompassing a wide range of colors and spatial patterns, is among the most noticeable features distinguishing species, individuals, and body regions within individuals. In holometabolous species, a significant portion of such diversity can be attributed to the melanin synthesis genes...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.186684
更新日期：2016-05-01 00:00:00
abstract：:We have analyzed two variants of Drosophila melanogaster (RS and RE) which lead to the dual phenotype of elevated DDC activity and increased resistance to dietary alpha-methyldopa relative to Oregon-R controls. Both phenotypes show tight genetic linkage to the dopa decarboxylase, Ddc, and l(2)amd genes (i.e., less tha...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1986-02-01 00:00:00
abstract：:The rate of adaptive evolution of a population ultimately depends on the rate of incorporation of beneficial mutations. Even beneficial mutations may, however, be lost from a population since mutant individuals may, by chance, fail to reproduce. In this paper, we calculate the probability of fixation of beneficial mut...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1997-06-01 00:00:00
