Abstract：
:l(3)S12 is a vital locus whose function is required in embryos, early larvae, late pupae and oogenesis. We have identified a cold-sensitive allele, l(3)S12(3), and characterized conditional misexpression of the gene associated with this mutation as well as with several euchromatic insertions of l(3)S12+ transposons. Surviving cold-sensitive mutants as well as underexpression variants generated by P element transformation display a phenotypic syndrome that can include delayed development, abnormal bristle morphology, and female sterility. Using these phenotypes, defects in putative "early" and "late" l(3)S12 expression can be identified. The sensitivity of certain l(3)S12+ insertions to site-specific euchromatic position effect appears to be due to separation of the gene from an endogenous enhancer element during cloning. This enhancerless construct can be used to identify and perhaps to select "permissive" euchromatic sites, presumably adjacent to enhancer elements, which in some cases permit elevated production not only of the l(3)S12 message, but also of a P element-l(3)S12 fusion transcript. Certain of these permissive sites appear to control stage-specific expression, and we propose that this system may be used to identify, clone, and characterize such loci. Heterochromatic position effect on this locus has been demonstrated. Available evidence suggests that the l(3)S12 gene may be involved in protein synthesis, perhaps encoding a ribosomal protein.
journal_name
Geneticsjournal_title
Geneticsauthors
Dutton FL Jr,Chovnick Akeywords:
subject
Has Abstractpub_date
1991-05-01 00:00:00pages
103-18issue
1eissn
0016-6731issn
1943-2631journal_volume
128pub_type
杂志文章相关文献
GENETICS文献大全abstract：:With the rise of both the number and the complexity of traits of interest, control of the false discovery rate (FDR) in genetic association studies has become an increasingly appealing and accepted target for multiple comparison adjustment. While a number of robust FDR-controlling strategies exist, the nature of this ...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.193987
更新日期：2017-01-01 00:00:00
abstract：:Thirty-five nucleotide polymorphisms were found in a 21.5-kbp region including the Est6 locus among 42 isoallelic lines extracted from a single natural population of Drosophila melanogaster. The heterozygosity per nucleotide pair was estimated to be 0.010 overall, but was lower in sequences hybridizing to transcripts ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1990-12-01 00:00:00
abstract：:Genomic sequences provide powerful new tools in genetic analysis, making it possible to combine classical genetics with genomics to characterize the genes in a particular chromosome region. These approaches have been applied successfully to the euchromatin, but analysis of the heterochromatin has lagged somewhat behin...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.166.2.807
更新日期：2004-02-01 00:00:00
abstract：:We consider the effect on the distribution of pairwise differences between mitochondrial DNA sequences of the incorporation into the underlying population genetics model of two particular effects that seem realistic for human populations. The first is that the population size was roughly constant before growing to its...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1994-02-01 00:00:00
abstract：:Fluorescent in situ hybridization was used to examine the distribution of six abundant long terminal repeat (LTR) retroelements, Opie, Huck, Cinful-1, Prem-2/Ji, Grande, and Tekay/Prem-1 on maize pachytene chromosomes. Retroelement staining in euchromatin was remarkably uniform, even when we included the structurally ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2003-10-01 00:00:00
abstract：:Variable numbers of tandem repeats (VNTRs) are a class of highly informative and widely dispersed genetic markers. Despite their wide application in biological science, little is known about their mutational mechanisms or population dynamics. The objective of this work was to investigate four summary measures of VNTR ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1993-07-01 00:00:00
abstract：:Following the advent of a gene targeting technique in Drosophila, different methods have been developed to modify the Drosophila genome. The initial demonstration of gene targeting in flies used an ends-in method, which generates a duplication of the target locus. The duplicated locus can then be efficiently reduced t...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.104.030882
更新日期：2004-11-01 00:00:00
abstract：:Currently used tests of Hardy-Weinberg proportions do not take into account the nature of the alternative hypothesis, which is generally a heterozygote deficiency. Different exact tests, appropriate for small sample size and large number of alleles, are proposed in this perspective, and their properties are evaluated ...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1995-08-01 00:00:00
abstract：:Isogenic lines, in which chromosomes sampled from natural populations of C. melanogaster are substituted into a common genetic background, were used to detect and partially characterize autosomal factors that affect the activities of the two pentose phosphate pathway enzymes, glucose 6-phosphate dehydrogenase (G6PD) a...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1981-09-01 00:00:00
abstract：:A consensus map for loblolly pine (Pinus taeda L.) was constructed from the integration of linkage data from two unrelated three-generation outbred pedigrees. The progeny segregation data from restriction fragment length polymorphism, random amplified polymorphic DNA, and isozyme genetic markers from each pedigree wer...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-01-01 00:00:00
abstract：:The decrease of meiotic exchanges (crossing over and conversion) in two mutants of Sordaria macrospora correlated strongly with a reduction of chiasmata and of both types of "recombination nodules." Serial section reconstruction electron microscopy was used to compare the synapsis pattern of meiotic prophase I in wild...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1992-09-01 00:00:00
abstract：:Clustering individuals to subpopulations based on genetic data has become commonplace in many genetic studies. Inference about population structure is most often done by applying model-based approaches, aided by visualization using distance-based approaches such as multidimensional scaling. While existing distance-bas...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.115.182626
更新日期：2016-04-01 00:00:00
abstract：:Genetic and developmental analysis of an X-linked vital locus vnd was undertaken. Embryos hemizygous for the original allele vnd did not hatch and exhibited a disorganized ventral nervous system (VNS). The mutation maps in the region 1B6-7 to 1B9-10, a subregion of an area previously shown to be essential to normal ne...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1983-07-01 00:00:00
abstract：:Phosphatidylinositol catabolism in Saccharomyces cerevisiae cells cultured in media containing inositol results in the release of glycerophosphoinositol (GroPIns) into the medium. As the extracellular concentration of inositol decreases with growth, the released GroPIns is transported back into the cell. Exploiting th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1998-08-01 00:00:00
abstract：:Genomic prediction methods based on multiple markers have potential to include nonadditive effects in prediction and analysis of complex traits. However, most developments assume a Hardy-Weinberg equilibrium (HWE). Statistical approaches for genomic selection that account for dominance and epistasis in a general conte...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.116.199406
更新日期：2017-07-01 00:00:00
abstract：:To understand the role of Los1p in pre-tRNA splicing, we sought los1 multicopy suppressors. We found SOL1 that suppresses both point and null LOS1 mutations. Since, when fused to the Ga14p DNA-binding domain, Los1p activates transcription, we tested whether Los1p regulates SOL1. We found that las1 mutants have deplete...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1996-06-01 00:00:00
abstract：:Peroxisomes are organelles containing a diverse array of enzymes. In fungi they are important for carbon source utilization, pathogenesis, development, and secondary metabolism. We have studied Aspergillus nidulans peroxin (pex) mutants isolated by virtue of their inability to grow on butyrate or by the inactivation o...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.107.085795
更新日期：2008-03-01 00:00:00
abstract：:Levels of genetic variation and linkage disequilibrium (LD) are critical factors in association mapping methods as well as in identification of loci that have been targets of selection. Maize, an outcrosser, has a high level of sequence variation and a limited extent of LD. Sorghum, a closely related but largely self-...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.167.1.471
更新日期：2004-05-01 00:00:00
abstract：:Spt3 of Saccharomyces cerevisiae is required for the normal transcription of many genes in vivo. Past studies have shown that Spt3 is required for both mating and sporulation, two events that initiate when cells are at G(1)/START. We now show that Spt3 is needed for two other events that begin at G(1)/START, diploid f...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-06-01 00:00:00
abstract：:Aimed at investigating the recovery of a specific mutant allele of the mating type locus (MAT) by switching a defective MAT allele, these experiments provide information bearing on several models proposed for MAT interconversion in bakers yeast, Saccharomyces cerevisiae. Hybrids between heterothallic (ho) cells carryi...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1979-07-01 00:00:00
abstract：:The organization of the mitochondrial genome in plants is not well understood. In maize mitochondrial DNA (mtDNA) several subgenomic circular molecules as well as an abundant fraction of linear molecules have been seen by electron microscopy. It has been hypothesized that the circular molecules are the genetic entitie...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1991-06-01 00:00:00
abstract：:Over one-third of human genome sequence is a product of non-LTR retrotransposition. The retrotransposon that currently drives this process in humans is the highly abundant LINE-1 (L1) element. Despite the ubiquitous nature of L1's in mammals, we still lack a complete mechanistic understanding of the L1 replication cyc...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.108.096636
更新日期：2009-01-01 00:00:00
abstract：:Pairwise linkage disequilibrium values (D) were estimated for 14 allozyme loci in two natural populations of lodgepole pine (Pinus contorta ssp. latifolia). Maternal multilocus genotypes were inferred from samples of (haploid) megagametophytic seed-endosperms. Coupling/repulsion double heterozygotes were distinguished...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1987-02-01 00:00:00
abstract：:Genetically marked P elements inserted into the subtelomeric satellites of Drosophila show repression and variegation of the reporter gene. One such white+ reporter, inserted between the subtelomeric satellite and the terminal HeT-A array in the left arm of chromosome 2 (2L), is sensitive to its context; changes in th...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2001-07-01 00:00:00
abstract：:The term "micro-evo-devo" refers to the combined study of the genetic and developmental bases of natural variation in populations and the evolutionary forces that have shaped this variation. It thus represents a synthesis of the fields of evolutionary developmental biology and population genetics. As has been pointed ...
journal_title：Genetics
pub_type： 杂志文章,评审
doi：10.1534/genetics.113.156463
更新日期：2013-11-01 00:00:00
abstract：:Cryptococcus neoformans is a fungal pathogen that causes meningitis in immunocompromised hosts. The organism has a known sexual cycle, and strains of the MATalpha mating type are more virulent than isogenic MATa strains in mice, and they are more common in the environment and infected hosts. A C. neoformans homolog of...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1999-12-01 00:00:00
abstract：:We demonstrate that recent data from human males are consistent with constant interference levels among chromosomes under the two-pathway model, whereas inappropriately fitting shape parameters of Gamma distributions to immunofluorescent interfoci distances observed on finite chromosomes generates false interpretation...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.109.103853
更新日期：2009-09-01 00:00:00
abstract：:The dmd gene of bacteriophage T4 is required for regulation of mRNA stability in a stage-dependent manner during infection. When this gene is mutated, late genes are globally silenced because of rapid degradation of mRNAs. To investigate the mechanism of such mRNA degradation, we analyzed the late gene soc transcripts...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：2002-01-01 00:00:00
abstract：:Properties of identity relation between genes are discussed, and a derivation of recurrent equations of identity coefficients in a random mating, diploid dioecious population is presented. Computations are run by repeated matrix multiplication. Results show that for effective population size (Ne) larger than 16 and no...
journal_title：Genetics
pub_type： 杂志文章
doi：
更新日期：1977-07-01 00:00:00
abstract：:Many arthropod species are infected with maternally inherited endosymbionts that induce a shift in the sex ratio of their hosts by feminizing or killing males (cytoplasmic sex-ratio distorters, or SRDs). These endosymbionts can have profound impacts on evolutionary processes of their hosts. Here, I derive analytical e...
journal_title：Genetics
pub_type： 杂志文章
doi：10.1534/genetics.110.120014
更新日期：2010-09-01 00:00:00
