Abstract:
:Genomic sequences provide powerful new tools in genetic analysis, making it possible to combine classical genetics with genomics to characterize the genes in a particular chromosome region. These approaches have been applied successfully to the euchromatin, but analysis of the heterochromatin has lagged somewhat behind. We describe a combined genetic and bioinformatics approach to the base of the right arm of the Drosophila melanogaster second chromosome, at the boundary between pericentric heterochromatin and euchromatin. We used resources provided by the genome project to derive a physical map of the region, examine gene density, and estimate the number of potential genes. We also carried out a large-scale genetic screen for lethal mutations in the region. We identified new alleles of the known essential genes and also identified mutations in 21 novel loci. Fourteen complementation groups map proximal to the assembled sequence. We used PCR to map the endpoints of several deficiencies and used the same set of deficiencies to order the essential genes, correlating the genetic and physical map. This allowed us to assign two of the complementation groups to particular "computed/curated genes" (CGs), one of which is Nipped-A, which our evidence suggests encodes Drosophila Tra1/TRRAP.
journal_name
Geneticsjournal_title
Geneticsauthors
Myster SH,Wang F,Cavallo R,Christian W,Bhotika S,Anderson CT,Peifer Mdoi
10.1534/genetics.166.2.807keywords:
subject
Has Abstractpub_date
2004-02-01 00:00:00pages
807-22issue
2eissn
0016-6731issn
1943-2631pii
166/2/807journal_volume
166pub_type
杂志文章相关文献
GENETICS文献大全abstract::The change in frequencies of D. melanogaster and D. simulans in competition experiments was used to measure the effect of radiation on the fitness of a population. A dose of 250 or 500 rads given to the males of highly inbred lines of D. simulans at the beginning of competition and every three weeks thereafter increas...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-11-01 00:00:00
abstract::Seed dormancy and germination in higher plants are partially controlled by the plant hormones abscisic acid (ABA) and gibberellic acid (GA). ABA establishes dormancy during embryo maturation, whereas GA breaks dormancy and induces germination. Previous attempts to identify GA response genes were confounded because GA ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-06-01 00:00:00
abstract::The genetic etiology of many complex diseases is highly heterogeneous. A complex disease can be caused by multiple mutations within the same gene or mutations in multiple genes at various genomic loci. Although these disease-susceptibility mutations can be collectively common in the population, they are often individu...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.118.301266
更新日期:2018-10-01 00:00:00
abstract::In mismatch repair (MMR), members of the MLH gene family have been proposed to act as key molecular matchmakers to coordinate mismatch recognition with downstream repair functions that result in mispair excision. Two members of this gene family, MLH1 and MLH3, have also been implicated in meiotic crossing over. These ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2002-03-01 00:00:00
abstract::The SUF13 and SUF14 genes were identified among extragenic suppressors of +1 frameshift mutations. SUF13 is synonymous with MBF1, a single-copy nonessential gene coding for a POLII transcription factor. The suf13-1 mutation is a two-nucleotide deletion in the SUF13/MBF1 coding region. A suf13::TRP1 null mutant suppres...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2001-03-01 00:00:00
abstract::A spontaneous antimycin A-resistant mutant carrying approximately four extra copies of ADH2 on chromosome XII was isolated from yeast strain 315-1D which lacks a functional copy of ADH1 and thus is antimycin A-sensitive. The additional copies of the normally glucose-repressed ADH2 are expressed during growth on glucos...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1992-02-01 00:00:00
abstract::A reversion study of EMS- or ICR170-induced ascospore color mutants in Ascobolus immersus is reported. Twenty-three new intragenic suppressors were isolated within the b2 locus. These are localized within three distinct groups on a genetic fine-structure map containing 21 identifiable sites. The pattern of reversion i...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1978-11-01 00:00:00
abstract::Dopamine acts through two classes of G protein-coupled receptor (D1-like and D2-like) to modulate neuron activity in the brain. While subtypes of D1- and D2-like receptors are coexpressed in many neurons of the mammalian brain, it is unclear how signaling by these coexpressed receptors interacts to modulate the activi...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.128512
更新日期:2011-07-01 00:00:00
abstract::Genetical and biochemical studies have been performed with revertants induced in a polyaromatic mutant (No. 58) in the arom gene cluster of Neurospora crassa. In addition to complete and partial revertants able to grow on minimal at both 25 degrees and 35 degrees , temperature-sensitive revertants capable of growth on...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1974-04-01 00:00:00
abstract::We investigated genetic polymorphism in the Caenorhabditis elegans srh and str chemoreceptor gene families, each of which consists of approximately 300 genes encoding seven-pass G-protein-coupled receptors. Almost one-third of the genes in each family are annotated as pseudogenes because of apparent functional defects...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.104.035329
更新日期:2005-04-01 00:00:00
abstract::The nucleotide sequences encoding the mature portion of 31 ribulose 1.5-bisphosphate carboxylase small subunit (SSU) genes from 17 genera of plants, green algae and cyanobacteria were examined. Among the 465 pairwise sequence comparisons, SSU multigene family members within the same species were more similar to each o...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1989-12-01 00:00:00
abstract::We use three allopatric populations of the stalk-eyed fly Teleopsis dalmanni from Southeast Asia to test two predictions made by the sex chromosome drive hypothesis for Haldane's rule. The first is that modifiers that suppress or enhance drive should evolve rapidly and independently in isolated populations. The second...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.167536
更新日期:2014-11-01 00:00:00
abstract::Gene expression in Anopheles gambiae shows a deficiency of testis-expressed genes on the X chromosome associated with an excessive movement of retrogene duplication. We suggest that the degeneration of sex chromosomes in this monandrous species is likely the result of pressures from X inactivation, dosage compensation...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.133157
更新日期:2011-11-01 00:00:00
abstract::In multicellular organisms, genetic programs guide cells to adopt cell fates as tissues are formed during development, maintained in adults, and repaired after injury. Here we explore how a small molecule in the environment can switch a genetic program from one fate to another. Wild-type Caenorhabditis elegans XX adul...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.169409
更新日期:2014-10-01 00:00:00
abstract::For multiallelic loci, standard measures of linkage disequilibrium provide an incomplete description of the correlation of variation at two loci, especially when there are different numbers of alleles at the two loci. We have developed a complementary pair of conditional asymmetric linkage disequilibrium (ALD) measure...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.114.165266
更新日期:2014-09-01 00:00:00
abstract::Gene silencing by heterochromatin is a well-known phenomenon that, in Drosophila, is called position effect variegation (PEV). The long-held hypothesis that this gene silencing is associated with an altered chromatin structure received direct support only recently. Another gene-silencing phenomenon in Drosophila, alth...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1998-12-01 00:00:00
abstract::Transposons can impact the host genome by altering gene expression and participating in chromosome rearrangements. Therefore, organisms evolved different ways to minimize the level of transposition. In Saccharomyces cerevisiae and its close relative S. paradoxus, Ty1 copy number control (CNC) is mediated by the self-e...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300388
更新日期:2017-12-01 00:00:00
abstract::We describe a method for co-estimating r = C/mu (where C is the per-site recombination rate and mu is the per-site neutral mutation rate) and Theta = 4N(e)mu (where N(e) is the effective population size) from a population sample of molecular data. The technique is Metropolis-Hastings sampling: we explore a large numbe...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2000-11-01 00:00:00
abstract::Sphingolipid signaling plays an important role in the regulation of central cellular processes, including cell growth, survival, and differentiation. Many of the essential pathways responsible for sphingolipid biogenesis, and key cellular responses to changes in sphingolipid balance, are conserved between mammalian an...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.106.064527
更新日期:2007-01-01 00:00:00
abstract::Aggression is a complex social behavior that is widespread in nature. To date, only a limited number of genes that affect aggression have been identified, in large part because the complexity of the phenotype makes screening difficult and time-consuming regardless of the species that is studied. We discovered that agg...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.117.300292
更新日期:2018-01-01 00:00:00
abstract::Transposable elements (TEs) constitute a substantial fraction of the eukaryotic genome and, as a result, have a complex relationship with their host that is both adversarial and dependent. To minimize damage to cellular genes, TEs possess mechanisms that target integration to sequences of low importance. However, the ...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.115.181602
更新日期:2015-11-01 00:00:00
abstract::We describe allelic variation at 28 gene loci in natural populations of D. willistoni. Seventy samples were studied from localities extending from Mexico and Florida, through Central America, the West Indies, and tropical South America, down to South Brazil. At least several hundred, and often several thousand, genome...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1972-01-01 00:00:00
abstract::The mutation process at microsatellite loci typically occurs at high rates and with stepwise changes in allele sizes, features that may introduce bias when using classical measures of population differentiation based on allele identity (e.g., F(ST), Nei's Ds genetic distance). Allele size-based measures of differentia...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-04-01 00:00:00
abstract::Two-dimensional paper chromatography was performed on methanol extracts of leaves of hexaploid bread wheat, Triticum aestivum L. em. Thell. cultivar Chinese Spring, and of the available nullisomic-tetrasomic compensating lines, the tetrasomic lines and the ditelocentric lines. The chromatograms had 27 spots identified...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1983-02-01 00:00:00
abstract::Cell differentiation in yeast species is controlled by a reversible, programmed DNA-rearrangement process called mating-type switching. Switching is achieved by two functionally similar but structurally distinct processes in the budding yeast Saccharomyces cerevisiae and the fission yeast Schizosaccharomyces pombe In ...
journal_title:Genetics
pub_type: 杂志文章,评审
doi:10.1534/genetics.117.202036
更新日期:2017-05-01 00:00:00
abstract::A second chromosome line of Drosophila melanogaster (Symbol: T-007) has previously been shown to be responsible for the induction of male recombination. In the present investigation, the genetic elements responsible for this phenomenon have been partially identified and mapped. A major element (Symbol: Mr, for Male re...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1975-10-01 00:00:00
abstract::The distribution of four X-linked mutants (G6PD, Deutan, Protan and Xg) among lowland and once highly malarial populations of Sardinia discloses a clear-cut example of linkage disequiligrium between two of them (G6PD and Protan). In the same populations the distribution of G6PD-deficiency versus colorblindness of the ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1977-05-01 00:00:00
abstract::The distribution of fitness effects (DFE) of new mutations is of fundamental importance in evolutionary genetics. Recently, methods have been developed for inferring the DFE that use information from the allele frequency distributions of putatively neutral and selected nucleotide polymorphic variants in a population s...
journal_title:Genetics
pub_type: 杂志文章
doi:10.1534/genetics.111.131730
更新日期:2011-12-01 00:00:00
abstract::In Xiphophorus, the causative genetic information for melanoma formation has been assigned by classical genetics to chromosomal loci, which are located on the sex chromosomes. In our attempts to molecularly clone these melanoma-determining loci, named Tu, we have looked for restriction-fragment-length markers (RFLMs) ...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:1988-07-01 00:00:00
abstract::We propose a new method for calculating probabilities for pedigree genetic data that incorporates crossover interference using the chi-square models. Applications include relationship inference, genetic map construction, and linkage analysis. The method is based on importance sampling of unobserved inheritance pattern...
journal_title:Genetics
pub_type: 杂志文章
doi:
更新日期:2003-08-01 00:00:00